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 | Acesso ao texto completo restrito à biblioteca da Embrapa Solos. Para informações adicionais entre em contato com cnps.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Solos. |
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Data corrente: |
20/09/2021 |
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Data da última atualização: |
20/09/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, V. M. de; MANFIO, G. P.; COUTINHO, H. L. da C.; KEIJZER-WOLTERS, A. C.; ELSAS, J. D. van. |
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Afiliação: |
VALÉRIA MAIA DE OLIVEIRA, UNICAMP; GILSON PAULO MANFIO, UNICAMP; HEITOR LUIZ DA COSTA COUTINHO, CNPS; ANNEKE CHRISTINA KEIJZER-WOLTERS, Plant Research International; JAN DIRK VAN ELSAS, University of Groningen. |
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Título: |
A ribosomal RNA gene intergenic spacer based PCR and DGGE fingerprinting method for the analysis of specific rhizobial communities in soil. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
Journal of Microbiological Methods, v. 64, n. 3, p. 366-379, Mar. 2006. |
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DOI: |
https://doi.org/10.1016/j.mimet.2005.05.015 |
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Idioma: |
Inglês |
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Conteúdo: |
A direct molecular method for assessing the diversity of specific populations of rhizobia in soil, based on nested PCR amplification of 16S-23S ribosomal RNA gene (rDNA) intergenic spacer (IGS) sequences, was developed. Initial generic amplification of bacterial rDNA IGS sequences from soil DNA was followed by specific amplification of (1) sequences affiliated with Rhizobium leguminosarum "sensu lato" and (2) R. tropici. Using analysis of the amplified sequences in clone libraries obtained on the basis of soil DNA, this two-sided method was shown to be very specific for rhizobial subpopulations in soil. It was then further validated as a direct fingerprinting tool of the target rhizobia based on denaturing gradient gel electrophoresis (DGGE). The PCR-DGGE approach was applied to soils from fields in Brazil cultivated with common bean (Phaseolus vulgaris) under conventional or no-tillage practices. The community fingerprints obtained allowed the direct analysis of the respective rhizobial community structures in soil samples from the two contrasting agricultural practices. Data obtained with both primer sets revealed clustering of the community structures of the target rhizobial types along treatment. Moreover, the DGGE profiles obtained with the R. tropici primer set indicated that the abundance and diversity of these organisms were favoured under NT practices. These results suggest that the R. leguminosarum-as well as R. tropici-targeted IGS-based nested PCR and DGGE are useful tools for monitoring the effect of agricultural practices on these and related rhizobial subpopulations in soils. MenosA direct molecular method for assessing the diversity of specific populations of rhizobia in soil, based on nested PCR amplification of 16S-23S ribosomal RNA gene (rDNA) intergenic spacer (IGS) sequences, was developed. Initial generic amplification of bacterial rDNA IGS sequences from soil DNA was followed by specific amplification of (1) sequences affiliated with Rhizobium leguminosarum "sensu lato" and (2) R. tropici. Using analysis of the amplified sequences in clone libraries obtained on the basis of soil DNA, this two-sided method was shown to be very specific for rhizobial subpopulations in soil. It was then further validated as a direct fingerprinting tool of the target rhizobia based on denaturing gradient gel electrophoresis (DGGE). The PCR-DGGE approach was applied to soils from fields in Brazil cultivated with common bean (Phaseolus vulgaris) under conventional or no-tillage practices. The community fingerprints obtained allowed the direct analysis of the respective rhizobial community structures in soil samples from the two contrasting agricultural practices. Data obtained with both primer sets revealed clustering of the community structures of the target rhizobial types along treatment. Moreover, the DGGE profiles obtained with the R. tropici primer set indicated that the abundance and diversity of these organisms were favoured under NT practices. These results suggest that the R. leguminosarum-as well as R. tropici-targeted IGS-based nested PCR and DGGE are us... Mostrar Tudo |
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Palavras-Chave: |
Culture-independent analysis; DGGE; Diversity; RDNA spacer; Rhizobia. |
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Thesaurus Nal: |
Soil management. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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Marc: |
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Registro original: |
Embrapa Solos (CNPS) |
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| Registros recuperados : 11 | |
| 4. |  | BAZZI, L. C.; MARTINS, R. M.; GEBLER, L.; SOUZA, G. E.; SCHENATTO, K.; SOBJAK, R.; HACHISUCA, A. M. M. Yield mapping in fruit farming. In: INTERNATIONAL CONFERENCE ON PRECISION AGRICULTURE, 15., 2022, Minnesota, USA. Anais... Minnesota: ISPA, 26 a 29 june 2022.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 5. | | SILVA, L. M. A.; ALVES FILHO, E. G.; MARTINS, R. M.; OLIVEIRA, J. D. J.; VIDAL, C. S.; OLIVEIRA, L. A. de; BRITO, E. s. de. NMR-based metabolomic approach for evaluation of the harvesting time and cooking characteristics of different cassava genotypes. Foods, v. 11, n. 11, 1651, p. 1-12, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
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| 6. | | PARPINELLI, R. S.; GRAÇA, J. P. da; SILVA, L. M.; MARTINS, R. M.; COTRIM, G.; TOLEDO, V. D.; CAMPO, C. B.; ZOCOLO, G.; GAZZONI, D. L. Monitoring Nectar Sugar in Nectaries During the Day and Night Periods of Soybean Flowers. In: SETAC LATIN AMERICA BIENNIAL MEETING, 14., 2021. Latin America, diversity of knowledge for a sustainable future: abstract book. Pensacola: SETAC, 2021. Virtual. p. 8.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 7. | | NASCIMENTO, K. B.; SACRAMENTO, J. P.; MARTINS, R. M; FERREIRA, A. L.; LIMA, J. A. M.; MACHADO, F. S.; PEREIRA, L. G. R.; TOMICH, T. R. Efeito da contagem de células somáticas sobre a composição de leite de vacas Girolando In: SIMPÓSIO DE QUALIDADE DO LEITE, 3., 2016, Jaboticabal. Resumos. Jaboticabal: Universidade Estadual Paulista, 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 8. | | NASCIMENTO, K. B.; SACRAMENTO, J. P.; FERREIRA, A.; MACHADO, F. S.; PEREIRA, L. G. R.; CAMPOS, M. M.; TOMICH, T. R.; MARTINS. R. M. Efeito da ordem de lactação e do nível de alimentação sobre a produção e composição do leite em vacas Girolando. In: SIMPÓSIO DE QUALIDADE DO LEITE, 3., 2016, Jaboticabal. Resumos. Jaboticabal: Universidade Estadual Paulista, 2016. p. 63.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 9. | | NASCIMENTO, K.; SACRAMENTO, J. P.; FERREIRA, A. I.; MACHADO, F. S.; PEREIRA, L. G. R.; TOMICH, T. R.; CAMPOS, M. M.; MARTINS, R. M. Consumo de nutrientes e produção de sólidos de vacas Girolando no terço médio da lactação. In: SIMPÓSIO DE QUALIDADE DO LEITE, 3., 2016, Jaboticabal. Resumos. Jaboticabal: Universidade Estadual Paulista, 2016. p. 63.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 10. | | XAVIER, M. da C.; PEIXER, M. A. S.; OLIVEIRA, L. G. A.; MALARD, P. F.; MARTINS, R. M.; ANDRADE, R. V.; VIANA, J. H. M. Use of a sperm separation device as an alternative for sires with low outcomes using gradient selection for in vitro embryo production: preliminary results. Animal Reproduction, v. 21, n. 3, 2024. Edição dos resumos da 37ª Annual Meeting of the Brazilian Embryo Technology Society, 2024, Atibaia, SP.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 11. | | TEIXEIRA, M. A.; MARTINS, R. M. S.; VIEIRA, R. F.; VILDOSO, C. I. A.; ADAMI, A. A. V.; FERREIRA, A. C. In vitro identification and control of Pestalotiopsis longisetula fungus, pathogens strawberry crop. Revista Agrogeoambiental, Pouso Alegre, v. 7, n. 3, p. 61-67, set. 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
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| Registros recuperados : 11 | |
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