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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
17/08/2021 |
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Data da última atualização: |
17/08/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LEAL, G. R.; GRACIOSA, M. A. G.; MONTEIRO, C. A. S.; PASOLINI, R.; CAMARGO, A. J. dos R.; OLIVEIRA, C. S.; VASCONCELOS, C. O. de P.; NOGUEIRA, L. A. G.; FERREIRA, A. M. R.; SERAPIAO, R. V. |
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Afiliação: |
GABRIELA RAMOS LEAL, Universidade Federal Fluminense; MARIA ALICE GUIMARAES GRACIOSA, Pesagro; CLARA ANA SANTOS MONTEIRO, Universidade Federal Fluminense; RENATA PASOLINI, Pesagro; AGOSTINHO JORGE DOS REIS CAMARGO, Pesagro; CLARA SLADE OLIVEIRA, CNPGL; CARLOS OTAVIO DE PAULA VASCONCELOS, Universidade Federal Fluminense; LUIZ ALTAMIRO GARCIA NOGUEIRA, Universidade Federal Fluminense; ANA MARIA REIS FERREIRA, Universidade Federal Fluminense; RAQUEL VARELLA SERAPIAO, Pesagro. |
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Título: |
The SPOM-adapted IVM system improves in vitro production of bovine embryos. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Theriogenology, v. 158, p. 277-282, 2020. |
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DOI: |
https://doi.org/10.1016/j.theriogenology.2020.09.024 |
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Idioma: |
Inglês |
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Conteúdo: |
This study aimed to test the effects of an IVM SPOM adaptation (SPOM-adapted IVM) on the production, total number of cells (TNC), apoptosis, and cryotolerance (post-warming survival and cytoskeleton actin integrity) of bovine IVP embryos. Two experiments were conducted with two experimental groups based on IVM treatment: A control group (TCM 199 without FCS) and an SPOM-adapted group (TCM 199 with forskolin and IBMX in pre-IVM and IVM with cilostamide). The first experiment evaluated embryo in vitro production, TNC, and apoptosis rate on D9 of development. In the second experiment, embryos were vitrified/warmed at D7 (control fresh and vitrified; SPOM-adapted fresh and vitrified) and assessed regarding post-warming survival rates and cytoskeleton actin integrity. Statistical analysis was performed using GraphPad INSTAT software at a significance level of 5%. An increase (p < 0.05) in blastocyst production was observed in the SPOM-adapted group comparing to the control group. There was no difference (p > 0.05) in the TNC or apoptosis rate between the groups. Regarding cryopreservation, no differences were found (p > 0.05) in actin integrity or post-warming survival rates between the vitrified groups. In both vitrified groups, we observed a significantly lower uninjured pattern of actin integrity compared to the fresh groups (p < 0.05). We conclude that the SPOM-adapted IVM system is beneficial for blastocyst production and does not affect the quality and cryotolerance of the produced embryos. MenosThis study aimed to test the effects of an IVM SPOM adaptation (SPOM-adapted IVM) on the production, total number of cells (TNC), apoptosis, and cryotolerance (post-warming survival and cytoskeleton actin integrity) of bovine IVP embryos. Two experiments were conducted with two experimental groups based on IVM treatment: A control group (TCM 199 without FCS) and an SPOM-adapted group (TCM 199 with forskolin and IBMX in pre-IVM and IVM with cilostamide). The first experiment evaluated embryo in vitro production, TNC, and apoptosis rate on D9 of development. In the second experiment, embryos were vitrified/warmed at D7 (control fresh and vitrified; SPOM-adapted fresh and vitrified) and assessed regarding post-warming survival rates and cytoskeleton actin integrity. Statistical analysis was performed using GraphPad INSTAT software at a significance level of 5%. An increase (p < 0.05) in blastocyst production was observed in the SPOM-adapted group comparing to the control group. There was no difference (p > 0.05) in the TNC or apoptosis rate between the groups. Regarding cryopreservation, no differences were found (p > 0.05) in actin integrity or post-warming survival rates between the vitrified groups. In both vitrified groups, we observed a significantly lower uninjured pattern of actin integrity compared to the fresh groups (p < 0.05). We conclude that the SPOM-adapted IVM system is beneficial for blastocyst production and does not affect the quality and cryotolerance of the ... Mostrar Tudo |
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Palavras-Chave: |
Cilostamida; Cilostamide. |
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Thesagro: |
Bovino; Criopreservação; Embrião Animal; Reprodução Animal. |
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Thesaurus Nal: |
Cryopreservation. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02485naa a2200325 a 4500
001 2133651
005 2021-08-17
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.theriogenology.2020.09.024$2DOI
100 1 $aLEAL, G. R.
245 $aThe SPOM-adapted IVM system improves in vitro production of bovine embryos.$h[electronic resource]
260 $c2020
520 $aThis study aimed to test the effects of an IVM SPOM adaptation (SPOM-adapted IVM) on the production, total number of cells (TNC), apoptosis, and cryotolerance (post-warming survival and cytoskeleton actin integrity) of bovine IVP embryos. Two experiments were conducted with two experimental groups based on IVM treatment: A control group (TCM 199 without FCS) and an SPOM-adapted group (TCM 199 with forskolin and IBMX in pre-IVM and IVM with cilostamide). The first experiment evaluated embryo in vitro production, TNC, and apoptosis rate on D9 of development. In the second experiment, embryos were vitrified/warmed at D7 (control fresh and vitrified; SPOM-adapted fresh and vitrified) and assessed regarding post-warming survival rates and cytoskeleton actin integrity. Statistical analysis was performed using GraphPad INSTAT software at a significance level of 5%. An increase (p < 0.05) in blastocyst production was observed in the SPOM-adapted group comparing to the control group. There was no difference (p > 0.05) in the TNC or apoptosis rate between the groups. Regarding cryopreservation, no differences were found (p > 0.05) in actin integrity or post-warming survival rates between the vitrified groups. In both vitrified groups, we observed a significantly lower uninjured pattern of actin integrity compared to the fresh groups (p < 0.05). We conclude that the SPOM-adapted IVM system is beneficial for blastocyst production and does not affect the quality and cryotolerance of the produced embryos.
650 $aCryopreservation
650 $aBovino
650 $aCriopreservação
650 $aEmbrião Animal
650 $aReprodução Animal
653 $aCilostamida
653 $aCilostamide
700 1 $aGRACIOSA, M. A. G.
700 1 $aMONTEIRO, C. A. S.
700 1 $aPASOLINI, R.
700 1 $aCAMARGO, A. J. dos R.
700 1 $aOLIVEIRA, C. S.
700 1 $aVASCONCELOS, C. O. de P.
700 1 $aNOGUEIRA, L. A. G.
700 1 $aFERREIRA, A. M. R.
700 1 $aSERAPIAO, R. V.
773 $tTheriogenology$gv. 158, p. 277-282, 2020.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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