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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
11/08/2021 |
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Data da última atualização: |
11/08/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GOGONE, I. C. V. P.; FERREIRA, G. H.; GAVA, D.; SCHAEFER, R.; PAULA-LOPES, F. F. de; ROCHA, R. de A.; BARROS, F. R. O. de. |
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Afiliação: |
IZABEL C. V. P. COGONE, UTFPR; GLAUCIA H. FERREIRA, UNIFESP/Diadema; DANIELLE GAVA, CNPSA; REJANE SCHAEFER, CNPSA; FABÍOLA F. DE PAULA-LOPES, UNIFESP/Diadema; RAQUEL DE A. ROCHA, UTFPR; FLAVIA REGINA OLIVEIRA DE BARROS, UTFPR. |
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Título: |
Applicability of Raman spectroscopy on porcine parvovirus and porcine circovirus type 2 detection. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, v. 249, n. 119336, 2021. |
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DOI: |
https://doi.org/10.1016/j.saa.2020.119336 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Porcine parvovirus (PPV) is one of the major infectious causes of reproductive failure of swine. This disease is characterized by embryonic and fetal infection and death, responsible for important economic losses. PPV is also implicated as a trigger in the development of post-weaning multisystemic wasting syndrome (PMWS) caused by Porcine circovirus type 2 (PCV2). Their detection is PCR-based, which is quite sensitive and specific, but laborious, costly and time-demanding. Therefore, this study aimed to assess Raman spectroscopy (RS) as a diagnostic tool for PPV and PCV2 due to its label-free properties and unique ability to search and identify molecular fingerprints. Briefly, swine testis (ST) cells were inoculated with PPV or PCV2 and in vitro cultured (37 C, 5% CO2) for four days. Fixed cells were then submitted to RS investigation using a 633 nm laser. A total of 225 spectra centered at 1300 cm1 was obtained for each sample (5 spectra/cell; 15 cells/replicate; 3 replicates) of PPV-, PCV2-infected and uninfected (control) ST cells. Clear statistical discrimination between samples from both virus-infected cells was achieved with a Principal Component ? Linear Discriminant Analysis (PCA-LDA) model, reaching sensitivity rates from 95.55% to 97.77%, respectively to PCV2- and PPV-infected cells. These results were then submitted to a Leave-One-Out (LOO) validation algorithm resulting in 99.97% of accuracy. Extensive band assignment was analyzed and compiled for better understanding of PPV and PCV2 virus-cell interaction, demonstrating that specific protein, lipids and DNA/RNA bands are the most important assignments related to discrimination of virus-infected from uninfected cells. In conclusion, these results represent promising bases for RS application on PCV2 and PPV detection for future diagnostic applications. MenosAbstract: Porcine parvovirus (PPV) is one of the major infectious causes of reproductive failure of swine. This disease is characterized by embryonic and fetal infection and death, responsible for important economic losses. PPV is also implicated as a trigger in the development of post-weaning multisystemic wasting syndrome (PMWS) caused by Porcine circovirus type 2 (PCV2). Their detection is PCR-based, which is quite sensitive and specific, but laborious, costly and time-demanding. Therefore, this study aimed to assess Raman spectroscopy (RS) as a diagnostic tool for PPV and PCV2 due to its label-free properties and unique ability to search and identify molecular fingerprints. Briefly, swine testis (ST) cells were inoculated with PPV or PCV2 and in vitro cultured (37 C, 5% CO2) for four days. Fixed cells were then submitted to RS investigation using a 633 nm laser. A total of 225 spectra centered at 1300 cm1 was obtained for each sample (5 spectra/cell; 15 cells/replicate; 3 replicates) of PPV-, PCV2-infected and uninfected (control) ST cells. Clear statistical discrimination between samples from both virus-infected cells was achieved with a Principal Component ? Linear Discriminant Analysis (PCA-LDA) model, reaching sensitivity rates from 95.55% to 97.77%, respectively to PCV2- and PPV-infected cells. These results were then submitted to a Leave-One-Out (LOO) validation algorithm resulting in 99.97% of accuracy. Extensive band assignment was analyzed and compiled for bette... Mostrar Tudo |
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Palavras-Chave: |
Molecular fingerprint; PCV2; PPV; Reproductive failure; Viral diagnostic. |
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Thesagro: |
Diagnostico; Parvovirose; Sanidade Animal; Suíno. |
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Thesaurus Nal: |
Animal diseases; Porcine circovirus-2; Raman spectroscopy; Reproductive success; Swine. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02976naa a2200373 a 4500
001 2133480
005 2021-08-11
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.saa.2020.119336$2DOI
100 1 $aGOGONE, I. C. V. P.
245 $aApplicability of Raman spectroscopy on porcine parvovirus and porcine circovirus type 2 detection.$h[electronic resource]
260 $c2021
520 $aAbstract: Porcine parvovirus (PPV) is one of the major infectious causes of reproductive failure of swine. This disease is characterized by embryonic and fetal infection and death, responsible for important economic losses. PPV is also implicated as a trigger in the development of post-weaning multisystemic wasting syndrome (PMWS) caused by Porcine circovirus type 2 (PCV2). Their detection is PCR-based, which is quite sensitive and specific, but laborious, costly and time-demanding. Therefore, this study aimed to assess Raman spectroscopy (RS) as a diagnostic tool for PPV and PCV2 due to its label-free properties and unique ability to search and identify molecular fingerprints. Briefly, swine testis (ST) cells were inoculated with PPV or PCV2 and in vitro cultured (37 C, 5% CO2) for four days. Fixed cells were then submitted to RS investigation using a 633 nm laser. A total of 225 spectra centered at 1300 cm1 was obtained for each sample (5 spectra/cell; 15 cells/replicate; 3 replicates) of PPV-, PCV2-infected and uninfected (control) ST cells. Clear statistical discrimination between samples from both virus-infected cells was achieved with a Principal Component ? Linear Discriminant Analysis (PCA-LDA) model, reaching sensitivity rates from 95.55% to 97.77%, respectively to PCV2- and PPV-infected cells. These results were then submitted to a Leave-One-Out (LOO) validation algorithm resulting in 99.97% of accuracy. Extensive band assignment was analyzed and compiled for better understanding of PPV and PCV2 virus-cell interaction, demonstrating that specific protein, lipids and DNA/RNA bands are the most important assignments related to discrimination of virus-infected from uninfected cells. In conclusion, these results represent promising bases for RS application on PCV2 and PPV detection for future diagnostic applications.
650 $aAnimal diseases
650 $aPorcine circovirus-2
650 $aRaman spectroscopy
650 $aReproductive success
650 $aSwine
650 $aDiagnostico
650 $aParvovirose
650 $aSanidade Animal
650 $aSuíno
653 $aMolecular fingerprint
653 $aPCV2
653 $aPPV
653 $aReproductive failure
653 $aViral diagnostic
700 1 $aFERREIRA, G. H.
700 1 $aGAVA, D.
700 1 $aSCHAEFER, R.
700 1 $aPAULA-LOPES, F. F. de
700 1 $aROCHA, R. de A.
700 1 $aBARROS, F. R. O. de
773 $tSpectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy$gv. 249, n. 119336, 2021.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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| Registros recuperados : 7 | |
| 1. |  | ABREU, C. S. de; CAMPOS, M. da S.; OLIVEIRA, R. S. de; MARINS, M. S.; GOMES, E. A.; STOIANOFF, M. A. de R.; OLIVEIRA-PAIVA, C. A. Efeito da adubação fosfatada sobre a população de micro-organismos solubilizadores e mineralizadores de fósforo isolados em rizosfera de milho. In: CONGRESSO NACIONAL DE MILHO E SORGO, 31., 2016, Bento Gonçalves. Milho e sorgo: inovações, mercados e segurança alimentar: anais. Sete Lagoas: Associação Brasileira de Milho e Sorgo, 2016.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 2. | | BONIEK, D.; MENDES, I. de C.; OLIVEIRA-PAIVA, C. A.; LANA, U. G. de P.; SANTOS, A. F. B. dos; STOIANOFF, M. A. de R. Ecology and identification of environmental fungi and metabolic processes involved in the biodeterioration of Brazilian soapstone historical monuments. Letters in Applied Microbiology, Oxford, v. 65, p. 431-438, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 3. | | CAMPOS, J. da S.; VITAL JÚNIOR, A. C.; BONIEK, D.; SILVA, D. L. da; LANA, U. G. de P.; FERNANDES, J. V.; STOIANOFF, M. A. de R.; ANDRADE, V. S. Identification and evaluation of thermotolerance of yeasts from milk in natura exposed to high temperature and slow and fast pasteurization. Brazilian Journal of Microbiology, v. 54, p. 1075-1082, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 4. | | ABREU, C. S. de; MARINS, M. S.; OLIVEIRA, R. S. de; CAMPOS, M. da S.; OLIVEIRA-PAIVA, C. A.; GOMES, E. A.; SANTOS, V. L. dos; MARRIEL, I. E.; STOIANOFF, M. A. de R. Atividade enzimática de fosfatases em solo cultivado com milho inoculado com micro-organismos solubilizadores de fosfato e adubado com fontes de fósforo de diferente solubilidade. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 32.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 16.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 14.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 11., 2016, Goiânia. Rumo aos novos desafios: [anais]. Viçosa, MG: Sociedade Brasileira de Ciência do Solo, 2016. p. 1025. FertBio 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 5. | | CONCEIÇÃO, R. R. P. da; QUEIROZ, V. A. V.; SIMEONE, M. L. F.; SILVA, D. D. da; CARMO, P. H. F. do; MENEZES, C. B. de; FIGUEIREDO, J. E. F.; STOIANOFF, M. A. de R. Does sorghum phenolic extract have antifungal effect? Brazilian Journal of Microbiology, v. 55, p. 1829-1839, 2024.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 6. | | ABREU, C. S. de; OLIVEIRA-PAIVA, C. A.; FIGUEIREDO, J. E. F.; TAVARES, A. N. G.; GOMES, E. A.; SANTOS, V. L. dos; MARRIEL, I. E.; STOIANOFF, M. A. de R. Seleção e caracterização de bactérias endofíticas de milho com potencial para biossolubilização de fosfato. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 32.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 16.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 14.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 11., 2016, Goiânia. Rumo aos novos desafios: [anais]. Viçosa, MG: Sociedade Brasileira de Ciência do Solo, 2016. p. 1004. FertBio 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 7. | | BONIEK, D.; OLIVEIRA, L. V. N. de; RIBEIRO, N. de Q.; OLIVEIRA-PAIVA, C. A.; LANA, U. G. de P.; SANTOS, A. F. B. dos; STOIANOFF, M. A. de R. Effect of environmental factors on rock-inhabiting fungal communities from brazilian soapstone samples. Journal of Environmental Science and Engineering B, v. 6, p. 55-71, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| Registros recuperados : 7 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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