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 | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
17/06/2021 |
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Data da última atualização: |
21/07/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BASSO, M. F.; COSTA, J. A.; RIBEIRO, T. P.; ARRAES, F. B. M.; LOURENCO, I. T.; MACEDO, A. F.; NEVES, M. R. das; NARDELI, S. M.; ARGE, L. W.; PEREZ, C. E. A.; SILVA, P. L. R.; MACEDO, L. L. P. de; LISEI-DE-SA, M. E.; AMORIM, R. M. S.; PINTO, E. R. de C.; SILVA, M. C. M. da; MORGANTE, C. V.; FLOH, E. I. S.; ALVES-FERREIRA, M.; SA, M. F. G. de. |
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Afiliação: |
MARCOS FERNANDO BASSO; JULIA ALMEIDA COSTA, UCB; THUANNE PIRES RIBEIRO, UNB; FABRICIO BARBOSA MONTEIRO ARRAES, UFRGS; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; AMANDA FERREIRA MACEDO, USP; MAYSA ROSA DAS NEVES; SARAH MUNIZ NARDELI, UFRJ; LUIS WILLIAN ARGE, UFRJ; CARLOS EDUARDO AUCIQUE PEREZ; PAOLO LUCAS RODRIGUES SILVA, UCB; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; MARIA EUGÊNIA LISEI-DE-SA, INCT PlantStress Biotech; REGINA MARIA SANTOS AMORIM; EDUARDO ROMANO DE CAMPOS PINTO, Cenargen; MARIA CRISTINA MATTAR DA SILVA, Cenargen; CAROLINA VIANNA MORGANTE, CPATSA; ENY IOCHEVET SEGAL FLOH, USP; MARCIO ALVES-FERREIRA, UFRJ; MARIA FATIMA GROSSI DE SA, Cenargen. |
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Título: |
Overexpression of the CaHB12 transcription factor in cotton (Gossypium hirsutum) improves drought tolerance. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Plant Physiology and Biochemistry, v. 165, p. 80-93, 2021. |
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DOI: |
https://doi.org/10.1016/j.plaphy.2021.05.009 |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Isabela Tristan Lourenço-Tessutti; Maria Cristina Mattar Silva; Maria Fatima Grossi-de-Sa. |
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Conteúdo: |
The Coffea arabica HB12 gene (CaHB12), which encodes a transcription factor belonging to the HD-Zip I sub-family, is upregulated under drought, and its constitutive overexpression (35S:CaHB12OX) improves the Arabi-dopsis thaliana tolerance to drought and salinity stresses. Herein, we generated transgenic cotton events constitutively overexpressing the CaHB12 gene, characterized these events based on their increased tolerance to water deficit, and exploited the gene expression level from the CaHB12 network. The segregating events Ev8.29.1, Ev8.90.1, and Ev23.36.1 showed higher photosynthetic yield and higher water use efficiency under severe water deficit and permanent wilting point conditions compared to wild-type plants. Under well-irrigated conditions, these three promising transformed events showed an equivalent level of Abscisic acid (ABA) and decreased Indole-3-acetic acid (IAA) accumulation, and a higher putrescine/(spermidine +spermine) ratio in leaf tissues was found in the progenies of at least two transgenic cotton events compared to non-transgenic plants. In addition, genes that are considered as modulated in the A. thaliana 35S:CaHB12OX line were also shown to be modulated in several transgenic cotton events maintained under field capacity conditions. The upregulation of GhPP2C and GhSnRK2 in transgenic cotton events maintained under permanent wilting point conditions suggested that CaHB12 might act enhancing the ABA-dependent pathway. All these data confirmed that CaHB12 overexpression improved the tolerance to water deficit, and the transcriptional modulation of genes related to the ABA signaling pathway or downstream genes might enhance the defense responses to drought. The observed decrease in IAA levels indicates that CaHB12 overexpression can prevent leaf abscission in plants under or after stress. Thus, our findings provide new insights on CaHB12 gene and identify several promising cotton events for conducting field trials on water deficit tolerance and agronomic performance. MenosThe Coffea arabica HB12 gene (CaHB12), which encodes a transcription factor belonging to the HD-Zip I sub-family, is upregulated under drought, and its constitutive overexpression (35S:CaHB12OX) improves the Arabi-dopsis thaliana tolerance to drought and salinity stresses. Herein, we generated transgenic cotton events constitutively overexpressing the CaHB12 gene, characterized these events based on their increased tolerance to water deficit, and exploited the gene expression level from the CaHB12 network. The segregating events Ev8.29.1, Ev8.90.1, and Ev23.36.1 showed higher photosynthetic yield and higher water use efficiency under severe water deficit and permanent wilting point conditions compared to wild-type plants. Under well-irrigated conditions, these three promising transformed events showed an equivalent level of Abscisic acid (ABA) and decreased Indole-3-acetic acid (IAA) accumulation, and a higher putrescine/(spermidine +spermine) ratio in leaf tissues was found in the progenies of at least two transgenic cotton events compared to non-transgenic plants. In addition, genes that are considered as modulated in the A. thaliana 35S:CaHB12OX line were also shown to be modulated in several transgenic cotton events maintained under field capacity conditions. The upregulation of GhPP2C and GhSnRK2 in transgenic cotton events maintained under permanent wilting point conditions suggested that CaHB12 might act enhancing the ABA-dependent pathway. All these data confirmed th... Mostrar Tudo |
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Palavras-Chave: |
ABA-Dependent pathway; Homeobox gene; Tolerancia a seca; Water deficit. |
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Thesagro: |
Algodão; Gossypium Hirsutum. |
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Thesaurus Nal: |
Cotton. |
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Categoria do assunto: |
--
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 03462naa a2200457 a 4500
001 2133063
005 2021-07-21
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.plaphy.2021.05.009$2DOI
100 1 $aBASSO, M. F.
245 $aOverexpression of the CaHB12 transcription factor in cotton (Gossypium hirsutum) improves drought tolerance.$h[electronic resource]
260 $c2021
500 $aNa publicação: Isabela Tristan Lourenço-Tessutti; Maria Cristina Mattar Silva; Maria Fatima Grossi-de-Sa.
520 $aThe Coffea arabica HB12 gene (CaHB12), which encodes a transcription factor belonging to the HD-Zip I sub-family, is upregulated under drought, and its constitutive overexpression (35S:CaHB12OX) improves the Arabi-dopsis thaliana tolerance to drought and salinity stresses. Herein, we generated transgenic cotton events constitutively overexpressing the CaHB12 gene, characterized these events based on their increased tolerance to water deficit, and exploited the gene expression level from the CaHB12 network. The segregating events Ev8.29.1, Ev8.90.1, and Ev23.36.1 showed higher photosynthetic yield and higher water use efficiency under severe water deficit and permanent wilting point conditions compared to wild-type plants. Under well-irrigated conditions, these three promising transformed events showed an equivalent level of Abscisic acid (ABA) and decreased Indole-3-acetic acid (IAA) accumulation, and a higher putrescine/(spermidine +spermine) ratio in leaf tissues was found in the progenies of at least two transgenic cotton events compared to non-transgenic plants. In addition, genes that are considered as modulated in the A. thaliana 35S:CaHB12OX line were also shown to be modulated in several transgenic cotton events maintained under field capacity conditions. The upregulation of GhPP2C and GhSnRK2 in transgenic cotton events maintained under permanent wilting point conditions suggested that CaHB12 might act enhancing the ABA-dependent pathway. All these data confirmed that CaHB12 overexpression improved the tolerance to water deficit, and the transcriptional modulation of genes related to the ABA signaling pathway or downstream genes might enhance the defense responses to drought. The observed decrease in IAA levels indicates that CaHB12 overexpression can prevent leaf abscission in plants under or after stress. Thus, our findings provide new insights on CaHB12 gene and identify several promising cotton events for conducting field trials on water deficit tolerance and agronomic performance.
650 $aCotton
650 $aAlgodão
650 $aGossypium Hirsutum
653 $aABA-Dependent pathway
653 $aHomeobox gene
653 $aTolerancia a seca
653 $aWater deficit
700 1 $aCOSTA, J. A.
700 1 $aRIBEIRO, T. P.
700 1 $aARRAES, F. B. M.
700 1 $aLOURENCO, I. T.
700 1 $aMACEDO, A. F.
700 1 $aNEVES, M. R. das
700 1 $aNARDELI, S. M.
700 1 $aARGE, L. W.
700 1 $aPEREZ, C. E. A.
700 1 $aSILVA, P. L. R.
700 1 $aMACEDO, L. L. P. de
700 1 $aLISEI-DE-SA, M. E.
700 1 $aAMORIM, R. M. S.
700 1 $aPINTO, E. R. de C.
700 1 $aSILVA, M. C. M. da
700 1 $aMORGANTE, C. V.
700 1 $aFLOH, E. I. S.
700 1 $aALVES-FERREIRA, M.
700 1 $aSA, M. F. G. de
773 $tPlant Physiology and Biochemistry$gv. 165, p. 80-93, 2021.
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