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Registro Completo |
Biblioteca(s): |
Embrapa Instrumentação. |
Data corrente: |
23/06/2021 |
Data da última atualização: |
10/06/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
BUFFO, M. M.; FERREIRA, A. L. Z.; ALMEIDA, R. M. R. G.; FARINAS, C. S.; BADINO, A. C.; XIMENES, E. A.; LADISCH, M. R. |
Afiliação: |
CRISTIANE SANCHEZ FARINAS, CNPDIA. |
Título: |
Cellulolytic enzymes production guided by morphology engineering. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Enzyme and Microbial Technology, v. 149, e109833, 2021. |
Páginas: |
1-8 |
ISSN: |
0141-0229 |
DOI: |
https://doi.org/10.1016/j.enzmictec.2021.109833 |
Idioma: |
Inglês |
Conteúdo: |
Endoglucanase and xylanase are critical enzymes for liquefaction and enzyme hydrolysis of high solids lignocellulosic biomass to facilitate its transport and production of desired derived products. Here is reported how combinations of different spore concentrations and pH influence microbial morphology, and how this may be used to direct expression and secretion of enzymes by Aspergillus niger. While xylanase production is not affected by A. niger morphology changes, endoglucanase production is enhanced under conditions of lower stress and by morphology that results in pellets. β-glucosidase production is enhanced under dispersed morphology, which results in up to fourfold increase of this enzyme production under the tested experimental conditions. A morphologic scale (Y) is proposed based on a form factor that considers the size and frequency of each morphology class, and that points to conditions that result in high selectivity for either endoglucanase or β-glucosidase production. An equation proposed to relate enzyme activity to morphology provides a useful tool for tuning enzyme production of A. niger, where morphology is a first indication of relative enzyme activities in a fermentation broth. |
Palavras-Chave: |
(hemi) Cellulases; Filamentous fungi; Morphology engineering. |
Categoria do assunto: |
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Marc: |
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2. |  | ALLQUERES, S.; MENESES, C.; ROTHBALLER, M.; MARTINS, O.; BALDANI, J. I.; SCHMID, M.; HARTMANN, A. The role of reactive oxygen species during gluconacetobacter diazotrophicus- rice interaction. INTERNATIONAL SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION WITH NON-LEGUMES, 13., 2012. Abstracts... Neuherberg: German Research Center for Environmental Health, 2012. 28Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Agrobiologia. |
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3. |  | HARTMANN, A.; JAMES, E. K.; BRUIJN, F. J. de; SCHWAB, S.; ROTHBALLER, M.; SCHMID, M. In situ localization and strain-specific quantification of Azospirillum and other dizotrophic plant plant growth promoting bacteria using antibodies and molecular probes. In: CASSÁN, F. D.; OKON, Y.; CREUS, C. M. (Ed.) Handbook for Azospirillum: techinical issues and protocols. Heidelberg: Springer, 2015 p. 45-64Tipo: Capítulo em Livro Técnico-Científico |
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4. |  | ALQUÉRES, S.; MENESES, C. H. G de; ROUWS, L. F. M.; ROTHBALLER, M.; BALDANI, J. I.; SCHMID, M.; HARTMANN, A. The bacterial superoxide dismutase and glutathione reductase are crucial for endophytic colonization of rice roots by Gluconacetobacter diazotrophicus PAL5. Molecular Plant-Microbe Interactions, v. 26, n. 8, p. 937-946, 2013.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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5. |  | HOFMANN, A.; RANKL, S.; HAN, S.; FISCHER, D.; SOARES, C. P.; VIDAL, M. S.; ROUWS, L. F. M.; BALDANI, J. I.; ROTHBALLER, M.; SCHRÖDER, P.; HARTMANN, A. Importance of quorum sensing signals in the communication of endophytic PGP-bacteria with their host plants. In: SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION, 16., LATINAMERICAN WORKSHOP OF PGPR, 4., RELARE, 19., 2018, Foz do Iguaçu. Book of abstract....Foz do Iguaçu: Emprapa, 2018. p. 39 Session 3, Short note III.IITipo: Resumo em Anais de Congresso |
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6. |  | FERREIRA, N. dos S.; SANTANNA, F. H.; REIS, V. M.; VOL´PIANO, C. G.; ROTHBALLER, M.; SCHWAB, S.; BAURA, V. A.; BALSANELLI, E.; PEDROSA, F. de OL; PASSAGLIA, L. M. P.; SOUZA, E. M. de; HARTMANN, A.; CASSAN, F.; ZILLI, J. E. Genome-based reclassification of Azospirillum brasilense Sp245 as the type strain of Azospirillum baldaniorum sp. nov. International Journal of Systematic and Evolutionary Microbiology, 15 Oct., 2020Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
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