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 | Acesso ao texto completo restrito à biblioteca da Embrapa Solos. Para informações adicionais entre em contato com cnps.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Solos. |
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Data corrente: |
29/01/2021 |
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Data da última atualização: |
26/10/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CARNEIRO, M. T.; PEREZ, D. V.; FEITOSA, R. C.; MACENA, L. G. P.; MIAGOSTOVICH, M. P.; WASSERMAN, J. C. |
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Afiliação: |
MARCOS T. CARNEIRO, UFF; DANIEL VIDAL PEREZ, CNPS; RENATO C. FEITOSA, FIOCRUZ; LORENA G. P. MACENA, FIOCRUZ; MARIZE P. MIAGOSTOVICH, FIOCRUZ; JÚLIO C. WASSERMAN, UFF. |
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Título: |
Escherichia coli capacity to repopulate microcosms under osmotic/U.V. synergic stress in tropical waters. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Current Microbiology, v. 78, n. 2, p. 756-764, Feb. 2021. |
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DOI: |
https://doi.org/10.1007/s00284-020-02319-2 |
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Idioma: |
Inglês |
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Conteúdo: |
In both Brazilian and European regulations, the impact assessment of sewage discharges into coastal waters is based on microbiological analyses of fecal indicators such as Escherichia coli, frequently used in prevision hydrodynamic models. However, the decay rates of E. coli vary depending on environmental conditions, and analysis may lead to inaccurate conclusions. This study aimed to analyze the decay of culturable and viable (but not culturable) E. coli in outdoor conditions, by creating microcosms inoculated with pre-treated sewage. The microcosms were filled with 9.88 L of filtered water (0.22 um membrane), 3.5% salt, 0.1-0.2% BHI, and 1% bacterial suspension obtained by reverse filtration. PMA-qPCR of E. coli uidA gene and Colilert measurements were applied to evaluate population counts after 2 h, 4 h, and 26 h. After nine hours of exposure to solar radiation, the viable cells decreased to 2.76% (interpolated value) of the initial population, and the cultivable fraction of the viable population accounted for 0.50%. In the dark period, the bacteria grew again, and viable cells reached 8.54%, while cultivable cells grew to 48.14% of initial population. This behavior is possibly due to the use of nutrients recycled from dead cells. Likewise, populations of E. coli in sewage outfalls remain viable in the sediments, where resuspension can renew blooming. |
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Palavras-Chave: |
Esgoto. |
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Thesagro: |
Decomposição; Escherichia Coli. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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Marc: |
LEADER 02101naa a2200229 a 4500
001 2129676
005 2021-10-26
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s00284-020-02319-2$2DOI
100 1 $aCARNEIRO, M. T.
245 $aEscherichia coli capacity to repopulate microcosms under osmotic/U.V. synergic stress in tropical waters.$h[electronic resource]
260 $c2021
520 $aIn both Brazilian and European regulations, the impact assessment of sewage discharges into coastal waters is based on microbiological analyses of fecal indicators such as Escherichia coli, frequently used in prevision hydrodynamic models. However, the decay rates of E. coli vary depending on environmental conditions, and analysis may lead to inaccurate conclusions. This study aimed to analyze the decay of culturable and viable (but not culturable) E. coli in outdoor conditions, by creating microcosms inoculated with pre-treated sewage. The microcosms were filled with 9.88 L of filtered water (0.22 um membrane), 3.5% salt, 0.1-0.2% BHI, and 1% bacterial suspension obtained by reverse filtration. PMA-qPCR of E. coli uidA gene and Colilert measurements were applied to evaluate population counts after 2 h, 4 h, and 26 h. After nine hours of exposure to solar radiation, the viable cells decreased to 2.76% (interpolated value) of the initial population, and the cultivable fraction of the viable population accounted for 0.50%. In the dark period, the bacteria grew again, and viable cells reached 8.54%, while cultivable cells grew to 48.14% of initial population. This behavior is possibly due to the use of nutrients recycled from dead cells. Likewise, populations of E. coli in sewage outfalls remain viable in the sediments, where resuspension can renew blooming.
650 $aDecomposição
650 $aEscherichia Coli
653 $aEsgoto
700 1 $aPEREZ, D. V.
700 1 $aFEITOSA, R. C.
700 1 $aMACENA, L. G. P.
700 1 $aMIAGOSTOVICH, M. P.
700 1 $aWASSERMAN, J. C.
773 $tCurrent Microbiology$gv. 78, n. 2, p. 756-764, Feb. 2021.
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