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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pantanal. Para informações adicionais entre em contato com cpap.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
05/01/2021 |
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Data da última atualização: |
25/02/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CURSINO, A. E.; LIMA, M. T.; NOGUEIRA, M. F.; AGUIAR, D. M. de; LUIZ, A. P. M. F.; ALVES, P. A.; ARAUJO JUNIOR, J. P.; KROON, E. G. |
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Afiliação: |
ANDREIA ELISA CURSINO, Universidade Federal de Minas Gerais; MAURÍCIO TEIXEIRA LIMA, Universidade Federal de Minas Gerais; MARCIA FURLAN NOGUEIRA T DE LIMA, CPAP; DANIEL MOURA DE AGUIAR, Universidade Federal de Mato Grosso; ANA PAULA MOREIRA FRANCO LUIZ, Viriontech do Brasil Indústria de Insumos e Serviços em Biotecnologia; PEDRO AUGUSTO ALVES, Instituto René Rachou, Fiocruz; JOÃO PESSOA ARAUJO JUNIOR, Universidade Estadual Paulista (Unesp); ERNA GEESSIEN KROON, Universidade Federal de Minas Gerais. |
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Título: |
Identification of large genetic variations in the equine infectious anemia virus tat-gag genomic region. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Transboundary and Emerging Diseases, p. 1-9, dez. 2020. |
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DOI: |
10.1111/tbed.13946 |
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Idioma: |
Inglês |
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Notas: |
Online First. |
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Conteúdo: |
Abstract: The aetiological agent of equine infectious anaemia (EIA) is the retrovirus equine infectious anemia virus (EIAV) that infects all members of the Equidae family. The EIA is widely disseminated in the Brazilian territory with a high seroprevalence in the Brazilian Pantanal and is mainly diagnosed using agar gel immunodiffusion (AGID). There are few complete EIAV genome sequences available in GenBank, which had an impact on molecular detection studies. In this study, we conducted molecular detection and sequencing of EIAV proviral DNA from Brazilian horses. We analysed the genomic region from exon 1 of tat to gag (tat-gag). Comparative serological tests, comprising AGID and two enzyme-linked immunosorbent assays (ELISAs), were also conducted. Of the 133 samples, 58 were positive in the tat-gag PCR, and 49 nucleotide sequences of 272 bp were obtained. Using this developed tat-gag PCR EIAV proviral DNA was detected in 7% of the AGID-negative samples and 26% of the AGID-negative samples were positive in at least one of the ELISA tests used. Using phylogenetic analysis, the Brazilian Pantanal EIAV sequences grouped in a different clade of EIAV sequences from other countries. Thus, the EIAV sequences can contribute to the knowledge of the tat-gag genomic region in the circulating viruses in the Brazilian Pantanal, in addition to providing new information about the genetic diversity. In addition, the serological results demonstrate the greater sensitivity of the ELISAs used in this study compared to AGID for EIA diagnosis. MenosAbstract: The aetiological agent of equine infectious anaemia (EIA) is the retrovirus equine infectious anemia virus (EIAV) that infects all members of the Equidae family. The EIA is widely disseminated in the Brazilian territory with a high seroprevalence in the Brazilian Pantanal and is mainly diagnosed using agar gel immunodiffusion (AGID). There are few complete EIAV genome sequences available in GenBank, which had an impact on molecular detection studies. In this study, we conducted molecular detection and sequencing of EIAV proviral DNA from Brazilian horses. We analysed the genomic region from exon 1 of tat to gag (tat-gag). Comparative serological tests, comprising AGID and two enzyme-linked immunosorbent assays (ELISAs), were also conducted. Of the 133 samples, 58 were positive in the tat-gag PCR, and 49 nucleotide sequences of 272 bp were obtained. Using this developed tat-gag PCR EIAV proviral DNA was detected in 7% of the AGID-negative samples and 26% of the AGID-negative samples were positive in at least one of the ELISA tests used. Using phylogenetic analysis, the Brazilian Pantanal EIAV sequences grouped in a different clade of EIAV sequences from other countries. Thus, the EIAV sequences can contribute to the knowledge of the tat-gag genomic region in the circulating viruses in the Brazilian Pantanal, in addition to providing new information about the genetic diversity. In addition, the serological results demonstrate the greater sensitivity of the ELISAs use... Mostrar Tudo |
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Thesagro: |
Anemia Infecciosa; Cavalo. |
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Thesaurus Nal: |
Equine infectious anemia virus; Horses. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02376naa a2200277 a 4500
001 2128970
005 2021-02-25
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1111/tbed.13946$2DOI
100 1 $aCURSINO, A. E.
245 $aIdentification of large genetic variations in the equine infectious anemia virus tat-gag genomic region.$h[electronic resource]
260 $c2020
500 $aOnline First.
520 $aAbstract: The aetiological agent of equine infectious anaemia (EIA) is the retrovirus equine infectious anemia virus (EIAV) that infects all members of the Equidae family. The EIA is widely disseminated in the Brazilian territory with a high seroprevalence in the Brazilian Pantanal and is mainly diagnosed using agar gel immunodiffusion (AGID). There are few complete EIAV genome sequences available in GenBank, which had an impact on molecular detection studies. In this study, we conducted molecular detection and sequencing of EIAV proviral DNA from Brazilian horses. We analysed the genomic region from exon 1 of tat to gag (tat-gag). Comparative serological tests, comprising AGID and two enzyme-linked immunosorbent assays (ELISAs), were also conducted. Of the 133 samples, 58 were positive in the tat-gag PCR, and 49 nucleotide sequences of 272 bp were obtained. Using this developed tat-gag PCR EIAV proviral DNA was detected in 7% of the AGID-negative samples and 26% of the AGID-negative samples were positive in at least one of the ELISA tests used. Using phylogenetic analysis, the Brazilian Pantanal EIAV sequences grouped in a different clade of EIAV sequences from other countries. Thus, the EIAV sequences can contribute to the knowledge of the tat-gag genomic region in the circulating viruses in the Brazilian Pantanal, in addition to providing new information about the genetic diversity. In addition, the serological results demonstrate the greater sensitivity of the ELISAs used in this study compared to AGID for EIA diagnosis.
650 $aEquine infectious anemia virus
650 $aHorses
650 $aAnemia Infecciosa
650 $aCavalo
700 1 $aLIMA, M. T.
700 1 $aNOGUEIRA, M. F.
700 1 $aAGUIAR, D. M. de
700 1 $aLUIZ, A. P. M. F.
700 1 $aALVES, P. A.
700 1 $aARAUJO JUNIOR, J. P.
700 1 $aKROON, E. G.
773 $tTransboundary and Emerging Diseases, p. 1-9, dez. 2020.
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Embrapa Pantanal (CPAP) |
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| 4. |  | CURSINO, A. E.; LIMA, M. T.; NOGUEIRA, M. F.; AGUIAR, D. M. de; LUIZ, A. P. M. F.; ALVES, P. A.; ARAUJO JUNIOR, J. P.; KROON, E. G. Identification of large genetic variations in the equine infectious anemia virus tat-gag genomic region. Transboundary and Emerging Diseases, p. 1-9, dez. 2020. Online First.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
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