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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sul. Para informações adicionais entre em contato com cppsul.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sul. |
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Data corrente: |
18/12/2020 |
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Data da última atualização: |
22/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LIMA, G. T. de; RODRIGUES, T. S.; PORTILHO, A. I.; CORREA, V. A.; GASPAR, E. B.; DE GASPARI, E. |
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Afiliação: |
Gabriela Trzewikoswki de Lima, USP; Thais Sousa Rodrigues, Adolfo Lutz Institute; Amanda Izeli Portilho, Adolfo Lutz Institute; Victor Araujo Correa, Adolfo Lutz Institute; EMANUELLE BALDO GASPAR, CPPSUL; Elizabeth De Gaspari, Adolfo Lutz Institute. |
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Título: |
Immune responses of meningococcal B outer membrane vesicles in middle-aged mice. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Pathogens and Disease, v. 78, n. 5, ftaa028, July, 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
The elderly are more likely to die when infected with Neisseria meningitidis. Aging is associated with immune system dysfunctions that impair responses to vaccines and infections. Therefore, immunization of middle-aged individuals could be beneficial. This study aims to evaluate the immunogenicity of N. meningitidis B outer membrane vesicles (OMVs) complexed to two different adjuvants. Middle-aged BALB/c and A/Sn mice were immunized and subsequent immune response was assessed by ELISA, immunoblotting and ELISpot. IgG levels were similar between the animals immunized with OMVs complexed to adjuvants. A total of 235 days after the last immunization only A/Sn mice presented higher IgG levels than those observed in the baseline, especially the group immunized with OMVs and aluminum hydroxide. The predominant IgG subclasses were IgG2a and IgG2b. Immunization with the three-dose regimen generated IgG antibodies that recognized a variety of antigens present in the homologous and heterologous meningococcal OMVs evaluated. There was an increase in the frequency of antigen-specific IFN-γ secreting splenocytes, after in vitro stimulation, in mice immunized with OMVs and adjuvants compared to the control group, almost 1 year after the last immunization. Both adjuvants showed similar performance. Immunization of middle-aged mice has generated a robust immune response and it appears to be advantageous. |
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Thesagro: |
Bactéria; Imunidade; Meningite. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02034naa a2200217 a 4500
001 2128434
005 2020-12-22
008 2020 bl uuuu u00u1 u #d
100 1 $aLIMA, G. T. de
245 $aImmune responses of meningococcal B outer membrane vesicles in middle-aged mice.$h[electronic resource]
260 $c2020
520 $aThe elderly are more likely to die when infected with Neisseria meningitidis. Aging is associated with immune system dysfunctions that impair responses to vaccines and infections. Therefore, immunization of middle-aged individuals could be beneficial. This study aims to evaluate the immunogenicity of N. meningitidis B outer membrane vesicles (OMVs) complexed to two different adjuvants. Middle-aged BALB/c and A/Sn mice were immunized and subsequent immune response was assessed by ELISA, immunoblotting and ELISpot. IgG levels were similar between the animals immunized with OMVs complexed to adjuvants. A total of 235 days after the last immunization only A/Sn mice presented higher IgG levels than those observed in the baseline, especially the group immunized with OMVs and aluminum hydroxide. The predominant IgG subclasses were IgG2a and IgG2b. Immunization with the three-dose regimen generated IgG antibodies that recognized a variety of antigens present in the homologous and heterologous meningococcal OMVs evaluated. There was an increase in the frequency of antigen-specific IFN-γ secreting splenocytes, after in vitro stimulation, in mice immunized with OMVs and adjuvants compared to the control group, almost 1 year after the last immunization. Both adjuvants showed similar performance. Immunization of middle-aged mice has generated a robust immune response and it appears to be advantageous.
650 $aBactéria
650 $aImunidade
650 $aMeningite
700 1 $aRODRIGUES, T. S.
700 1 $aPORTILHO, A. I.
700 1 $aCORREA, V. A.
700 1 $aGASPAR, E. B.
700 1 $aDE GASPARI, E.
773 $tPathogens and Disease$gv. 78, n. 5, ftaa028, July, 2020.
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