| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Meio Ambiente. |
|
Data corrente: |
04/11/2020 |
|
Data da última atualização: |
12/04/2021 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
MATIOLLI, C. C.; CHISTE, B. M.; TAKESHITA, N. A.; JONSSON, C. M.; FERRACINI, V. L.; HISANO, H. |
|
Afiliação: |
CRISTIANO CAMPOS MATIOLLI, CNPq; BRUNA MILKE CHISTE; NATALIA AKEMI TAKESHITA, Unifaj; CLAUDIO MARTIN JONSSON, CNPMA; VERA LUCIA FERRACINI, CNPMA; HAMILTON HISANO, CNPMA. |
|
Título: |
Acute toxicity and risk assessment of florfenicol for Nile tilapia larvae. |
|
Ano de publicação: |
2020 |
|
Fonte/Imprenta: |
Bulletin of Environmental Contamination and Toxicology, v. 105, n. 5, p. 721-727, 2020. |
|
ISSN: |
0007-4861 |
|
DOI: |
https://doi.org/10.1007/s00128-020-03013-6 |
|
Idioma: |
Português |
|
Conteúdo: |
Abstract: The aim of this study was to determine the median lethal concentration (LC50-96h), effective concentration (EC50-96h), risk assessment, and development of Nile tilapia Oreochromis niloticus larvae submitted to florfenicol (FF) exposure. Fish (n-147; 8.6 ± 0.6 mg; 7 fish/aquarium) were randomly distributed in 21 aquaria (1 L) and exposed to five concentrations of FF 58.73; 131.31; 198.96; 241.88 and 381.81 mg L-1 plus one control and a control with solvent, totalizing seven treatments and three replicates. The estimated median LC50-96h of FF for Nile tilapia larvae was 349.94 mg L-1. The EC50-96h of FF was 500 mg L-1 for weight reduction and was 1040 mg L-1 for length reduction. After the exposure period, final weight and length differed (p < 0.05) among treatments, showing the lowest biometric values with the highest concentrations of FF. The pH and dissolved oxygen were altered (p < 0.05) during the experimental period. The FF high doses used to determine the LC 50 after 96 h negatively affected the development of the larvae. On the other hand, through risk assessment analysis this antibiotic can be classified as low toxicity to Nile tilapia larvae and show low environmental risk. |
|
Thesagro: |
Antibiótico; Aquicultura; Impacto Ambiental; Piscicultura; Tilápia; Tilápia Nilótica. |
|
Thesaurus Nal: |
Antibiotics; Aquaculture; Ecotoxicology; Environmental impact; Tilapia (common name). |
|
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
|
Marc: |
LEADER 02224naa a2200337 a 4500
001 2126300
005 2021-04-12
008 2020 bl uuuu u00u1 u #d
022 $a0007-4861
024 7 $ahttps://doi.org/10.1007/s00128-020-03013-6$2DOI
100 1 $aMATIOLLI, C. C.
245 $aAcute toxicity and risk assessment of florfenicol for Nile tilapia larvae.$h[electronic resource]
260 $c2020
520 $aAbstract: The aim of this study was to determine the median lethal concentration (LC50-96h), effective concentration (EC50-96h), risk assessment, and development of Nile tilapia Oreochromis niloticus larvae submitted to florfenicol (FF) exposure. Fish (n-147; 8.6 ± 0.6 mg; 7 fish/aquarium) were randomly distributed in 21 aquaria (1 L) and exposed to five concentrations of FF 58.73; 131.31; 198.96; 241.88 and 381.81 mg L-1 plus one control and a control with solvent, totalizing seven treatments and three replicates. The estimated median LC50-96h of FF for Nile tilapia larvae was 349.94 mg L-1. The EC50-96h of FF was 500 mg L-1 for weight reduction and was 1040 mg L-1 for length reduction. After the exposure period, final weight and length differed (p < 0.05) among treatments, showing the lowest biometric values with the highest concentrations of FF. The pH and dissolved oxygen were altered (p < 0.05) during the experimental period. The FF high doses used to determine the LC 50 after 96 h negatively affected the development of the larvae. On the other hand, through risk assessment analysis this antibiotic can be classified as low toxicity to Nile tilapia larvae and show low environmental risk.
650 $aAntibiotics
650 $aAquaculture
650 $aEcotoxicology
650 $aEnvironmental impact
650 $aTilapia (common name)
650 $aAntibiótico
650 $aAquicultura
650 $aImpacto Ambiental
650 $aPiscicultura
650 $aTilápia
650 $aTilápia Nilótica
700 1 $aCHISTE, B. M.
700 1 $aTAKESHITA, N. A.
700 1 $aJONSSON, C. M.
700 1 $aFERRACINI, V. L.
700 1 $aHISANO, H.
773 $tBulletin of Environmental Contamination and Toxicology$gv. 105, n. 5, p. 721-727, 2020.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Meio Ambiente (CNPMA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Agroenergia. |
|
Data corrente: |
23/09/2019 |
|
Data da última atualização: |
23/09/2019 |
|
Tipo da produção científica: |
Artigo em Anais de Congresso |
|
Autoria: |
GREGORINE, L. F.; MONCLARO, A. V.; SILVA, C. de O. G.; RODRIGUES, K. B.; PEIXOTO, J. S. G.; ABDELNUR, P. V.; FAVARO, L. C. de L. |
|
Afiliação: |
Lucas F. Gregorine; Antonielle V. Monclaro; Caio de Oliveira Gorgulho Silva, Consultor da Embrapa Agroenergia; Kelly B. Rodrigues; Jéssica S. G. Peixoto, UnB; PATRICIA VERARDI ABDELNUR, CNPAE; LEIA CECILIA DE LIMA FAVARO, CNPAE. |
|
Título: |
Heterologous expression of a new lytic polysaccharide monooxygenase from Hahella ganghwensis and their functional characterization. |
|
Ano de publicação: |
2019 |
|
Fonte/Imprenta: |
In: SIMPÓSIO NACIONAL DE BIOPROCESSOS, 22.; SIMPÓSIO DE HIDRÓLISE ENZIMÁTICA DE BIOMASSA, 13., 2019, Uberlandia, MG. [Anais ...]. São Paulo: Associação Brasileira de Engenharia Química, 2019. |
|
Idioma: |
Inglês |
|
Conteúdo: |
The powerful class of oxidative enzymes, lytic polysaccharide monooxygenases (LPMOs) - also named Auxiliary Activity (AA) - are able to oxidize recalcitrant polysaccharides on lignocellulosic biomass. In this work, we successfully expressed three catalytic domains from bacterial LPMOs in the yeast Komagataella phaffii: domain MdAA10.1-SD (from Moritella dasanensis), domain VmAA10.2-SD (from Verrucosispora maris), and domain HgAA10.1-SD (from Hahella ganghwensis). Heterologous expression was analyzed by SDS-PAGE, Western-Blot, and Dot-Blot, while functional activity of these proteins was investigated by a combination of mass spectrometric and chromatographic methods. The recombinant LPMO catalytic domain HgAA10.1-SD from H. ganghwensis, a C1-oxidizer, was able to promote an oxidative cleavage of phosphoric-acid swollen cellulose (PASC) substrate in the presence of ascorbic acid as an electron donor, showing its potential for cellulose depolymerization. |
|
Palavras-Chave: |
Chromatographic; Spectrometric. |
|
Categoria do assunto: |
-- |
|
URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/1112393/1/107193786998fieldsubmissionfulltextfile1.pdf
|
|
Marc: |
LEADER 01787nam a2200205 a 4500
001 2112393
005 2019-09-23
008 2019 bl uuuu u00u1 u #d
100 1 $aGREGORINE, L. F.
245 $aHeterologous expression of a new lytic polysaccharide monooxygenase from Hahella ganghwensis and their functional characterization.$h[electronic resource]
260 $aIn: SIMPÓSIO NACIONAL DE BIOPROCESSOS, 22.; SIMPÓSIO DE HIDRÓLISE ENZIMÁTICA DE BIOMASSA, 13., 2019, Uberlandia, MG. [Anais ...]. São Paulo: Associação Brasileira de Engenharia Química$c2019
520 $aThe powerful class of oxidative enzymes, lytic polysaccharide monooxygenases (LPMOs) - also named Auxiliary Activity (AA) - are able to oxidize recalcitrant polysaccharides on lignocellulosic biomass. In this work, we successfully expressed three catalytic domains from bacterial LPMOs in the yeast Komagataella phaffii: domain MdAA10.1-SD (from Moritella dasanensis), domain VmAA10.2-SD (from Verrucosispora maris), and domain HgAA10.1-SD (from Hahella ganghwensis). Heterologous expression was analyzed by SDS-PAGE, Western-Blot, and Dot-Blot, while functional activity of these proteins was investigated by a combination of mass spectrometric and chromatographic methods. The recombinant LPMO catalytic domain HgAA10.1-SD from H. ganghwensis, a C1-oxidizer, was able to promote an oxidative cleavage of phosphoric-acid swollen cellulose (PASC) substrate in the presence of ascorbic acid as an electron donor, showing its potential for cellulose depolymerization.
653 $aChromatographic
653 $aSpectrometric
700 1 $aMONCLARO, A. V.
700 1 $aSILVA, C. de O. G.
700 1 $aRODRIGUES, K. B.
700 1 $aPEIXOTO, J. S. G.
700 1 $aABDELNUR, P. V.
700 1 $aFAVARO, L. C. de L.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Agroenergia (CNPAE) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
