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Registro Completo |
Biblioteca(s): |
Embrapa Suínos e Aves. |
Data corrente: |
04/11/2020 |
Data da última atualização: |
04/11/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ESPÍNDOLA, J. P.; BALBINOTT, N.; GRESSLER, L. T.; MACHADO, G.; KLEIN, C. S.; REBELATTO, R.; MARTÍN, C. B. G.; KREUTZ, L. C.; SCHRYVERS, A. B.; FRANDOLOSO, R. |
Afiliação: |
JÚLIA PIRES ESPÍNDOLA, UPF; NATÁLIA BALBINOTT, UPF; LETÍCIA TREVISAN GRESSLER, UPF; GUSTAVO MACHADO, North Carolina State University; CATIA SILENE KLEIN, CNPSA; RAQUEL REBELATTO, CNPSA; CÉSAR BERNARDO GUTIÉRREZ MARTÍN, University of León; LUIZ CARLOS KREUTZ, UPF; ANTHONY BERNARD SCHRYVERS, University of Calgary; RAFAEL FRANDOLOSO, UPF. |
Título: |
Molecular serotyping of clinical strains of Haemophilus (Glaesserella) parasuis brings new insights regarding Glassers disease outbreaks in Brazil. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
PeerJ, v. 7, n. e6817, 2019. |
DOI: |
http://doi.org/10.7717/peerj.6817 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Glässer's disease (GD) is an important infectious disease of swine caused by Haemophilus (Glaesserella) parasuis. Vaccination with inactivated whole cell vaccines is the major approach for prevention of H. parasuis infection worldwide, but the immunity induced is predominantly against the specific polysaccharide capsule. As a consequence, the available vaccines may not induce adequate protection against the field strains, when the capsules present in the vaccine strains are different from those in strains isolated from the farms. Therefore, it is crucial to map H. parasuis serovars associated with regional outbreaks so that appropriate bacterin vaccines can be developed and distributed for prevention of infection. In this study, 459 H. parasuis field strains isolated from different Glässer's disease outbreaks that occurred in 10 different Brazilian States were analyzed for serotype using PCR-based approaches. Surprisingly, non-typeable (NT) strains were the second most prevalent group of field strains and along with serovars 4, 5 and 1 comprised more than 70% of the isolates. A PCRbased approach designed to amplify the entire polysaccharide capsule locus revealed 9 different band patterns in the NT strains, and 75% of the NT strains belonged to three clusters, suggesting that a number of new serovars are responsible for a substantial proportion of disease. These results indicate that commercially available vaccines in Brazil do not cover the most prevalent H. parasuis serovars associated with GD. Disease mapping, Haemophilus parasuis, Typification, Serovars, Vaccines, Brazil Microbiology, Veterinary Medicine MenosAbstract: Glässer's disease (GD) is an important infectious disease of swine caused by Haemophilus (Glaesserella) parasuis. Vaccination with inactivated whole cell vaccines is the major approach for prevention of H. parasuis infection worldwide, but the immunity induced is predominantly against the specific polysaccharide capsule. As a consequence, the available vaccines may not induce adequate protection against the field strains, when the capsules present in the vaccine strains are different from those in strains isolated from the farms. Therefore, it is crucial to map H. parasuis serovars associated with regional outbreaks so that appropriate bacterin vaccines can be developed and distributed for prevention of infection. In this study, 459 H. parasuis field strains isolated from different Glässer's disease outbreaks that occurred in 10 different Brazilian States were analyzed for serotype using PCR-based approaches. Surprisingly, non-typeable (NT) strains were the second most prevalent group of field strains and along with serovars 4, 5 and 1 comprised more than 70% of the isolates. A PCRbased approach designed to amplify the entire polysaccharide capsule locus revealed 9 different band patterns in the NT strains, and 75% of the NT strains belonged to three clusters, suggesting that a number of new serovars are responsible for a substantial proportion of disease. These results indicate that commercially available vaccines in Brazil do not cover the most prevalent H. paras... Mostrar Tudo |
Palavras-Chave: |
Brasil; Disease mapping; Serovares; Serovars; Tipificação; Typification. |
Thesagro: |
Microbiologia; Vacina; Veterinária. |
Thesaurus Nal: |
Brazil; Haemophilus parasuis; Microbiology; Vaccines; Veterinary medicine. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02802naa a2200409 a 4500 001 2126299 005 2020-11-04 008 2019 bl uuuu u00u1 u #d 024 7 $ahttp://doi.org/10.7717/peerj.6817$2DOI 100 1 $aESPÍNDOLA, J. P. 245 $aMolecular serotyping of clinical strains of Haemophilus (Glaesserella) parasuis brings new insights regarding Glassers disease outbreaks in Brazil.$h[electronic resource] 260 $c2019 520 $aAbstract: Glässer's disease (GD) is an important infectious disease of swine caused by Haemophilus (Glaesserella) parasuis. Vaccination with inactivated whole cell vaccines is the major approach for prevention of H. parasuis infection worldwide, but the immunity induced is predominantly against the specific polysaccharide capsule. As a consequence, the available vaccines may not induce adequate protection against the field strains, when the capsules present in the vaccine strains are different from those in strains isolated from the farms. Therefore, it is crucial to map H. parasuis serovars associated with regional outbreaks so that appropriate bacterin vaccines can be developed and distributed for prevention of infection. In this study, 459 H. parasuis field strains isolated from different Glässer's disease outbreaks that occurred in 10 different Brazilian States were analyzed for serotype using PCR-based approaches. Surprisingly, non-typeable (NT) strains were the second most prevalent group of field strains and along with serovars 4, 5 and 1 comprised more than 70% of the isolates. A PCRbased approach designed to amplify the entire polysaccharide capsule locus revealed 9 different band patterns in the NT strains, and 75% of the NT strains belonged to three clusters, suggesting that a number of new serovars are responsible for a substantial proportion of disease. These results indicate that commercially available vaccines in Brazil do not cover the most prevalent H. parasuis serovars associated with GD. Disease mapping, Haemophilus parasuis, Typification, Serovars, Vaccines, Brazil Microbiology, Veterinary Medicine 650 $aBrazil 650 $aHaemophilus parasuis 650 $aMicrobiology 650 $aVaccines 650 $aVeterinary medicine 650 $aMicrobiologia 650 $aVacina 650 $aVeterinária 653 $aBrasil 653 $aDisease mapping 653 $aSerovares 653 $aSerovars 653 $aTipificação 653 $aTypification 700 1 $aBALBINOTT, N. 700 1 $aGRESSLER, L. T. 700 1 $aMACHADO, G. 700 1 $aKLEIN, C. S. 700 1 $aREBELATTO, R. 700 1 $aMARTÍN, C. B. G. 700 1 $aKREUTZ, L. C. 700 1 $aSCHRYVERS, A. B. 700 1 $aFRANDOLOSO, R. 773 $tPeerJ$gv. 7, n. e6817, 2019.
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1. | | ESPÍNDOLA, J. P.; BALBINOTT, N.; GRESSLER, L. T.; MACHADO, G.; KLEIN, C. S.; REBELATTO, R.; MARTÍN, C. B. G.; KREUTZ, L. C.; SCHRYVERS, A. B.; FRANDOLOSO, R. Molecular serotyping of clinical strains of Haemophilus (Glaesserella) parasuis brings new insights regarding Glassers disease outbreaks in Brazil. PeerJ, v. 7, n. e6817, 2019.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Suínos e Aves. |
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2. | | Di DOMENÍCO, J.; CANOVA, R.; SOVERAL, L. de F.; NIED, C. O.; COSTA, M. M.; FRANDOLOSO, R.; KREUTZ, L. C. Immunomodulatory effects of dietary B-glucan in silver catfish (Rhamdia quelen). Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 1, p. 73-78, janeiro. 2017.Biblioteca(s): Embrapa Unidades Centrais. |
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3. | | KREUTZ, L. C.; CANOVA, R.; NIED, C. O.; BORTOLUZZI, M.; FRANDOLOSO, R. Characterization of an IgM-like immunoglobulin from silver catfish (Rhamdia quelen) serum and its use for the production of polyclonal antibodies and development of immunoassays. Pesquisa Veterinária Brasileira, Brasília, DF. V. 36, N. 9, p. 819-825, set. 2016.Biblioteca(s): Embrapa Unidades Centrais. |
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Registros recuperados : 3 | |
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