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 | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
14/06/2020 |
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Data da última atualização: |
16/06/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RIBEIRO, J. M.; TEIXEIRA, S. L.; SOUZA, J. C. de; RIBEIRO, B. L.; OLIVEIRA, A. B. N.; CARNEIRO NETO, T. F. de S.; RIOS, L. S.; VIEIRA, R. dos S. |
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Afiliação: |
JULIANA MARTINS RIBEIRO, CPATSA; Silvio Lopes Teixeira; Joselita Cardoso de Souza; Brenda Lima Ribeiro; Antônio Bruno Nunes Oliveira; Thiago Francisco de Souza Carneiro Neto; Lucas Silva Rios; Reivandson dos Santos Vieira. |
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Título: |
In vitro response of date palm (Phoenix dactylifera L.) inflorescence. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Australian Journal of Crop Science, v. 14, n. 5, p. 831-835, 2020. |
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DOI: |
10.21475/ajcs.20.14.05.p2317 |
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Idioma: |
Inglês |
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Conteúdo: |
One of the major problems related to the implementation of date palm crops in Brazil is propagation. Therefore, the aim of this study was to evaluate the possibility of using tissue culture technique for the in vitro propagation of this species. Hence, the effect of 2iP and 2,4-D on the in vitro response of date palm inflorescence tissues, related to floral bud swelling, callusing, and rhizogenesis, was evaluated. The absence of 2,4-D was more detrimental to the in vitro response of inflorescence bud explants than absence of 2iP. In treatments without addition of 2,4-D to the culture medium, explants did not have swelling, callus or root formation. The treatment containing 150 mg/L 2,4-D in the presence of 1.5 mg/L 2iP initiated explant swelling, and treatments with either 100 mg/L or 150 mg/L 2,4-D, combined with 3.0 mg/L 2iP, were also efficient in stimulating in vitro swelling of inflorescence buds. Rhizogenesis was induced at the highest concentrations of 2,4-D (100 and 150 mg/L), combined with 4.5 mg/L 2iP, and was visually more evident in the treatment containing 150 mg/L 2,4-D + 4.5 mg/L 2iP. These results suggest that even higher concentrations of these two reagents might be efficient in the micropropagation of new existing date palm genotypes in the Submedium São Francisco River Valley. |
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Palavras-Chave: |
Citocinina; Rizogênese. |
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Thesagro: |
Auxina; Cultura de Tecido; Micropropagação; Phoenix Dactylifera; Tâmara. |
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Thesaurus Nal: |
Auxins; Cytokinins; Micropropagation; Rhizogenesis. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02304naa a2200349 a 4500
001 2123273
005 2020-06-16
008 2020 bl uuuu u00u1 u #d
024 7 $a10.21475/ajcs.20.14.05.p2317$2DOI
100 1 $aRIBEIRO, J. M.
245 $aIn vitro response of date palm (Phoenix dactylifera L.) inflorescence.$h[electronic resource]
260 $c2020
520 $aOne of the major problems related to the implementation of date palm crops in Brazil is propagation. Therefore, the aim of this study was to evaluate the possibility of using tissue culture technique for the in vitro propagation of this species. Hence, the effect of 2iP and 2,4-D on the in vitro response of date palm inflorescence tissues, related to floral bud swelling, callusing, and rhizogenesis, was evaluated. The absence of 2,4-D was more detrimental to the in vitro response of inflorescence bud explants than absence of 2iP. In treatments without addition of 2,4-D to the culture medium, explants did not have swelling, callus or root formation. The treatment containing 150 mg/L 2,4-D in the presence of 1.5 mg/L 2iP initiated explant swelling, and treatments with either 100 mg/L or 150 mg/L 2,4-D, combined with 3.0 mg/L 2iP, were also efficient in stimulating in vitro swelling of inflorescence buds. Rhizogenesis was induced at the highest concentrations of 2,4-D (100 and 150 mg/L), combined with 4.5 mg/L 2iP, and was visually more evident in the treatment containing 150 mg/L 2,4-D + 4.5 mg/L 2iP. These results suggest that even higher concentrations of these two reagents might be efficient in the micropropagation of new existing date palm genotypes in the Submedium São Francisco River Valley.
650 $aAuxins
650 $aCytokinins
650 $aMicropropagation
650 $aRhizogenesis
650 $aAuxina
650 $aCultura de Tecido
650 $aMicropropagação
650 $aPhoenix Dactylifera
650 $aTâmara
653 $aCitocinina
653 $aRizogênese
700 1 $aTEIXEIRA, S. L.
700 1 $aSOUZA, J. C. de
700 1 $aRIBEIRO, B. L.
700 1 $aOLIVEIRA, A. B. N.
700 1 $aCARNEIRO NETO, T. F. de S.
700 1 $aRIOS, L. S.
700 1 $aVIEIRA, R. dos S.
773 $tAustralian Journal of Crop Science$gv. 14, n. 5, p. 831-835, 2020.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
04/02/2009 |
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Data da última atualização: |
22/07/2011 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Internacional - A |
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Autoria: |
ABREU, S. R. de O.; MOTA, R. A.; ROSINHA, G. M. S.; FORNER, O.; PINHEIRO JÚNIOR, J. W.; PEREIRA, R. R. B.; CASTRO, R. S. de; ELISEI, C.; SOARES, C. O.; ARAÚJO, F. R.; MADUREIRA, R. C. |
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Afiliação: |
Sílvio Romero de O. Abreu, CESMAC; Rinaldo A. Mota, UFRPB; Grácia Maria Soares Rosinha, CNPGC; Odinéia Forner, bolsista CNPGC; José W. Pinheiro Júnior, UFRPB; Renata Ribeiro Bastos Pereira, bolsista CNPGC; Roberto S. de Castro, UFRPR; Carina Elisei, Pesquisadora DCR do CNPq/FUNDECT no CNPGC; Cleber Oliveira Soares, CNPGC; Flábio Ribeiro Araújo, CNPGC; Renata Cunha Madureira, CNPGC. |
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Título: |
Comparação genotípica de isolados de Corynebacterium pseudotuberculosis de caprinos e ovinos do sertão de Pernambuco. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Seropédica, v. 28, n. 10, p. 481-487, out. 2008. |
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Idioma: |
Português |
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Conteúdo: |
Resumo: Objetivou-se com este estudo comparar genotipicamente 35 isolados de Corynebacterium pseudotuberculosis recuperados de conteúdo de abscessos de caprinos e ovinos com linfadenite caseosa, procedentes de cinco municípios localizados no Sertão de Pernambuco, Brasil. Utilizou-se a técnica de fingerprint RFLP-PCR com as enzimas de restrição Hpy-Ch4 e Msp1 aplicada ao gene rpoB e as enzimas Pst I e Msp I para o gene pld. Não houve diferença nos padrões de fragmentos de bandas entre os isolados, independente da espécie hospedeira ou da área geográfica estudada, definindo-se um padrão genotípico homogêneo de C. pseudotuberculosis responsável por abscessos superficiais na região.
[Genotypic comparison between Corynebacterium pseudotuberculosis samples obtained from sheep and goats with caseous lymphadenitis, raised in the semi-arid region of Pernambuco].
Abstract: The objective was to genotypically compare 35 samples of Corynebacterium pseudotuberculosis obtained from abscesses of sheep and goats diagnosed with caseous lymphadenitis originated from 5 different municipalities in the semi-arid region of Pernambuco, Brazil. The RFLP-PCR technique with Hpy-Ch4 and Msp I and Pst I Msp I restriction enzimes was used to fingerprint the genes rpoB and pld, respectively. The results demonstrate that there was no difference on the fragments banding pattern among samples, independently of the host species or geographic area studied, defining a homogeneous profile of C. pseudotuberculosis responsible for superficial abscesses for the region. MenosResumo: Objetivou-se com este estudo comparar genotipicamente 35 isolados de Corynebacterium pseudotuberculosis recuperados de conteúdo de abscessos de caprinos e ovinos com linfadenite caseosa, procedentes de cinco municípios localizados no Sertão de Pernambuco, Brasil. Utilizou-se a técnica de fingerprint RFLP-PCR com as enzimas de restrição Hpy-Ch4 e Msp1 aplicada ao gene rpoB e as enzimas Pst I e Msp I para o gene pld. Não houve diferença nos padrões de fragmentos de bandas entre os isolados, independente da espécie hospedeira ou da área geográfica estudada, definindo-se um padrão genotípico homogêneo de C. pseudotuberculosis responsável por abscessos superficiais na região.
[Genotypic comparison between Corynebacterium pseudotuberculosis samples obtained from sheep and goats with caseous lymphadenitis, raised in the semi-arid region of Pernambuco].
Abstract: The objective was to genotypically compare 35 samples of Corynebacterium pseudotuberculosis obtained from abscesses of sheep and goats diagnosed with caseous lymphadenitis originated from 5 different municipalities in the semi-arid region of Pernambuco, Brazil. The RFLP-PCR technique with Hpy-Ch4 and Msp I and Pst I Msp I restriction enzimes was used to fingerprint the genes rpoB and pld, respectively. The results demonstrate that there was no difference on the fragments banding pattern among samples, independently of the host species or geographic area studied, defining a homogeneous profile of C. pseudotuberculos... Mostrar Tudo |
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Palavras-Chave: |
Pernambuco; RFLP-PCR. |
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Thesagro: |
Caprino; Corynebacterium pseudotuberculosis; Epidemiologia; Linfadenite caseosa; Ovino; Pseudotuberculose; Sanidade animal. |
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Categoria do assunto: |
--
H Saúde e Patologia |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/326874/1/v28n10a07.pdf
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Marc: |
LEADER 02603naa a2200349 a 4500
001 1326874
005 2011-07-22
008 2008 bl uuuu u00u1 u #d
100 1 $aABREU, S. R. de O.
245 $aComparação genotípica de isolados de Corynebacterium pseudotuberculosis de caprinos e ovinos do sertão de Pernambuco.
260 $c2008
520 $aResumo: Objetivou-se com este estudo comparar genotipicamente 35 isolados de Corynebacterium pseudotuberculosis recuperados de conteúdo de abscessos de caprinos e ovinos com linfadenite caseosa, procedentes de cinco municípios localizados no Sertão de Pernambuco, Brasil. Utilizou-se a técnica de fingerprint RFLP-PCR com as enzimas de restrição Hpy-Ch4 e Msp1 aplicada ao gene rpoB e as enzimas Pst I e Msp I para o gene pld. Não houve diferença nos padrões de fragmentos de bandas entre os isolados, independente da espécie hospedeira ou da área geográfica estudada, definindo-se um padrão genotípico homogêneo de C. pseudotuberculosis responsável por abscessos superficiais na região. [Genotypic comparison between Corynebacterium pseudotuberculosis samples obtained from sheep and goats with caseous lymphadenitis, raised in the semi-arid region of Pernambuco]. Abstract: The objective was to genotypically compare 35 samples of Corynebacterium pseudotuberculosis obtained from abscesses of sheep and goats diagnosed with caseous lymphadenitis originated from 5 different municipalities in the semi-arid region of Pernambuco, Brazil. The RFLP-PCR technique with Hpy-Ch4 and Msp I and Pst I Msp I restriction enzimes was used to fingerprint the genes rpoB and pld, respectively. The results demonstrate that there was no difference on the fragments banding pattern among samples, independently of the host species or geographic area studied, defining a homogeneous profile of C. pseudotuberculosis responsible for superficial abscesses for the region.
650 $aCaprino
650 $aCorynebacterium pseudotuberculosis
650 $aEpidemiologia
650 $aLinfadenite caseosa
650 $aOvino
650 $aPseudotuberculose
650 $aSanidade animal
653 $aPernambuco
653 $aRFLP-PCR
700 1 $aMOTA, R. A.
700 1 $aROSINHA, G. M. S.
700 1 $aFORNER, O.
700 1 $aPINHEIRO JÚNIOR, J. W.
700 1 $aPEREIRA, R. R. B.
700 1 $aCASTRO, R. S. de
700 1 $aELISEI, C.
700 1 $aSOARES, C. O.
700 1 $aARAÚJO, F. R.
700 1 $aMADUREIRA, R. C.
773 $tPesquisa Veterinária Brasileira, Seropédica$gv. 28, n. 10, p. 481-487, out. 2008.
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