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Registro Completo |
Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
08/06/2020 |
Data da última atualização: |
12/06/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
CAMPESTRINI, L. H.; RASERA, G. B.; CAMARGO, A. C. de; FRANCHIN, M.; NANI, B. D.; ROSALEN, P. L.; CANNIATTI-BRAZACA, S. G.; BIASOTO, A. C. T.; SHAHIDIC, F.; ALENCAR, S. M. |
Afiliação: |
Luciano Henrique Campestrini; Gabriela Boscariol Rasera; Adriano Costa de Camargo; Marcelo Franchin; Bruno Dias Nani; Pedro Luiz Rosalen; Solange Guidolin Canniatti-Brazaca; ALINE TELLES BIASOTO MARQUES, CPATSA; Fereidoon Shahidic; Severino Matias Alencar. |
Título: |
Alkaline conditions better extract anti-inflammatory polysaccharides from winemaking by-products. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Food Research International, v. 131, may 2020. |
DOI: |
https://doi.org/10.1016/j.foodres.2019.108532 |
Idioma: |
Inglês |
Conteúdo: |
Winemaking generates large amounts of by-products, a well recognized source of phenolic compounds. However, less attention has been paid to the polysaccharide-rich fraction (PRF) and effects of fractionation techniques on its potential bioactivity. Therefore, PRFs from Syrah and Tempranillo winemaking by-products were extracted under aqueous (neutral pH conditions), acidic and alkaline conditions. PRFs were screened for their monosaccharide composition, uronic acid content, homogeneity and molecular weight. Anti-inflammatory activity of PRFs were evaluated on stimulated RAW 264.7 macrophages. PRF obtained in water and/or under acidic conditions showed heterogeneous profiles. As like as in the others, a heterogeneous and complex profile was detected in extracts procured under alkaline conditions. A high content of uronic acid was found in aqueous extracts, thus indicating the presence of pectin. Pectin and hemicellulose were present in PRFs procured under acidic conditions. Alkaline conditions rendered extracts containing a complex mixture of monosaccharides, mainly xylose. This latter PRF was the only one exhibiting anti-inflammatory potential (at 100 ?g/mL) by reducing the release of TNF-? and activation of NF-?B in LPS-activated RAW 264.7 macrophages, with no effect on cell viability. Regardless of the grape variety, PRFs obtained under alkaline conditions were the best option to obtain bioactive polysaccharides with potential application as a source of anti-inflammatory compounds. A complex mixture of polymers may be responsible for the anti-inflammatory effects. Finally, according to results procured by NMR, it is possible to suggest that bioactive fractions are composed of a chain of ?-L-Araf-(1 ? 3) linked, ?-D-Xylp- (1 ? 4), ?-D-Glcp-(1 ? 4) linked, ?-D-GalpA-(1 ? 4), ?-D-Gal-(1 ? 2) forming possible RG I and RG II and xylan chains. MenosWinemaking generates large amounts of by-products, a well recognized source of phenolic compounds. However, less attention has been paid to the polysaccharide-rich fraction (PRF) and effects of fractionation techniques on its potential bioactivity. Therefore, PRFs from Syrah and Tempranillo winemaking by-products were extracted under aqueous (neutral pH conditions), acidic and alkaline conditions. PRFs were screened for their monosaccharide composition, uronic acid content, homogeneity and molecular weight. Anti-inflammatory activity of PRFs were evaluated on stimulated RAW 264.7 macrophages. PRF obtained in water and/or under acidic conditions showed heterogeneous profiles. As like as in the others, a heterogeneous and complex profile was detected in extracts procured under alkaline conditions. A high content of uronic acid was found in aqueous extracts, thus indicating the presence of pectin. Pectin and hemicellulose were present in PRFs procured under acidic conditions. Alkaline conditions rendered extracts containing a complex mixture of monosaccharides, mainly xylose. This latter PRF was the only one exhibiting anti-inflammatory potential (at 100 ?g/mL) by reducing the release of TNF-? and activation of NF-?B in LPS-activated RAW 264.7 macrophages, with no effect on cell viability. Regardless of the grape variety, PRFs obtained under alkaline conditions were the best option to obtain bioactive polysaccharides with potential application as a source of anti-inflammatory c... Mostrar Tudo |
Palavras-Chave: |
Atividade anti-inflamatória; Extração sequencial; Polissacarídeos; Vinificação. |
Thesagro: |
Pectina; Subproduto; Uva. |
Thesaurus Nal: |
Grapes; Polysaccharides. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
Marc: |
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
Data corrente: |
24/02/2011 |
Data da última atualização: |
31/10/2019 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
GEBLER, L.; BERTOL, I.; RAMOS, R. R.; BARBOSA, F. T.; SCHRAMMEL, B. M.; LOUZADA, J. A. S. |
Afiliação: |
LUCIANO GEBLER, CNPUV; ILDEGARDIS BERTOL, UDESC; ROGER ROBERT RAMOS, UDESC; FABRÍCIO TONDELLO BARBOSA, UDESC; BRUNA MOREIRA SCHRAMMEL, UCS; JOSÉ ANTÔNIO SALDANHA LOUZADA, UFRGS/IPH. |
Título: |
Arraste superficial de fósforo reativo em pequenas bacias hidrográficas sob diferentes tipos de manejo agrícola e possível impacto nos recursos hídricos e no sistema de gestão: uma proposta preliminar. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
In: CONGRESO INTERAMERICANO DE INGENIERIA SANITARIA Y AMBIENTAL, 32.; FORO INTERAMERICANO SOBRE SERVICIOS DE AGUA Y SANEAMENTO, 2., 2010, Bávaro, República Dominicana. El cumplimento de las metas del milenio en la busqueda de la universalizacion de los servicios. [S.l.]: AIDIS, 2010. |
Páginas: |
p. 1-7. |
Descrição Física: |
1 CD-ROM. |
Idioma: |
Português |
Conteúdo: |
The behavior of dissolved reactive phosphorus in the soi! may depend on the different systems of soi! management, providing a timely management of impacts on the environment. The aim of this study was to assess lhe form of drag and reactive phosphorus in different types of ground cover offered and suggest alternatives to the traditional management in agricultural are as ofthe basins. The study was conducted in Lages, Se, between the November/2009 março/2010, a lnceptsoil under jive different types of crops randomly distributed, with two replications. There were three tests of simulated rain with 90 minutes in duration and intensity of 64 mm h-I, using rainfall simulator rotating boom type Swanson. The tests were carried out at 30, 69 and 105 days afier sowing. Losses of phosphorus in runoff varied with rainfall tests, but not with the types of farming system. The plots with soybean and corn were the bachelor results closer than expected, whi!e the plots of bare soi! and bean showed the highest difference of the expected behavior in relation to the behavior of reactive phosphorus in comparison to the total reactive phosphorus jiltrado. Com base this, it is suggested that management of small rural watersheds take into account the type of management that will be given to the ground in order to adapt where necessary physical structures to contain runof!. Likewise, the management should take into account the times that offer greater risk, which would be the time of planting and harvest, depending on the way it is made. KeyWords: Reactive Agricultural contaminants, agricultural environmental mamagement, risk indicator MenosThe behavior of dissolved reactive phosphorus in the soi! may depend on the different systems of soi! management, providing a timely management of impacts on the environment. The aim of this study was to assess lhe form of drag and reactive phosphorus in different types of ground cover offered and suggest alternatives to the traditional management in agricultural are as ofthe basins. The study was conducted in Lages, Se, between the November/2009 março/2010, a lnceptsoil under jive different types of crops randomly distributed, with two replications. There were three tests of simulated rain with 90 minutes in duration and intensity of 64 mm h-I, using rainfall simulator rotating boom type Swanson. The tests were carried out at 30, 69 and 105 days afier sowing. Losses of phosphorus in runoff varied with rainfall tests, but not with the types of farming system. The plots with soybean and corn were the bachelor results closer than expected, whi!e the plots of bare soi! and bean showed the highest difference of the expected behavior in relation to the behavior of reactive phosphorus in comparison to the total reactive phosphorus jiltrado. Com base this, it is suggested that management of small rural watersheds take into account the type of management that will be given to the ground in order to adapt where necessary physical structures to contain runof!. Likewise, the management should take into account the times that offer greater risk, which would be the time of planting and h... Mostrar Tudo |
Palavras-Chave: |
Gestão ambiental; Indicador de risco. |
Thesagro: |
Agricultura; Contaminação; Fósforo; Manejo; Meio ambiente; Recurso hídrico. |
Categoria do assunto: |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/878857/1/129692010.pdf
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Embrapa Uva e Vinho (CNPUV) |
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