| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Gado de Leite. |
|
Data corrente: |
29/12/2019 |
|
Data da última atualização: |
06/02/2024 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
WELLER, M. M. D. C. A.; FONSECA, I.; SBARDELLA, A. P.; PINTO, I. S. B.; VICCINI, L. F.; BRANDAO, H. de M.; GERN, J. C.; CARVALHO, W. A.; GUIMARÃES, A. S.; BRITO, M. A. V. P.; MUNARI, D. P.; SILVA, M. V. G. B.; MARTINS, M. F. |
|
Afiliação: |
HUMBERTO DE MELLO BRANDAO, CNPGL; JULIANA CARINE GERN, CNPGL; WANESSA ARAUJO CARVALHO, CNPGL; ALESSANDRO DE SA GUIMARAES, CNPGL; MARCOS VINICIUS GUALBERTO B SILVA, CNPGL; MARTA FONSECA MARTINS, CNPGL. |
|
Título: |
Isolated perfused udder model for transcriptome analysis in response to Streptococcus agalactiae. |
|
Ano de publicação: |
2019 |
|
Fonte/Imprenta: |
Journal of Dairy Research, v. 86, n. 3, p. 307-314, 2019. |
|
DOI: |
https://doi.org/10.1017/s0022029919000451 |
|
Idioma: |
Inglês |
|
Conteúdo: |
This study aimed to evaluate the transcriptional changes occurring in isolated perfused mammary alveolar tissue in response to inoculation with S. agalactiae and to identify the most affected biological functions and pathways after 3 h. Four udders taken at slaughter from cows with healthy mammary gland were perfused ex situ with warmed and gassed Tyrode's solution. Mammary alveolar tissue samples were taken from the left fore and rear quarters (IQ-inoculated quarters) before inoculation (hour 0) and at 3 h post inoculation (hpi) and at the same times from control right fore and rear quarters (not inoculated: NIQ). A total of 1756 differentially expressed genes (DEGs) were identified between IQ and NIQ at 3 hpi using edgeR package. Within this set of DEGs, 952 were up regulated and mainly involved with innate immune response and inflammatory response, e.g., CD14, CCL5, TLR2, IL-8, SAA3, as well as in transcriptional regulation such as FOS, STAT3 and NFKBIA. Genes down-regulated (804) included those involved with lipid synthesis e.g., APOC2, SCD, FABP3 and FABP4. The most affected pathways were chemokine signaling, Wnt signaling and complement and coagulation cascades, which likely reflects the early stage response of mammary tissue to S. agalactiae infection. No significant gene expression changes were detected by RNA-Seq in the others contrasts. Real time-PCR confirmed the increase in mRNA abundance of immune-related genes: TLR2, TLR4, IL-1β, and IL-10 at 3 hpi between IQ and NIQ. The expression profiles of Casp1 and Bax for any contrasts were unaffected whereas Bcl2 was increased in IQ, which suggests no induction of apoptosis during the first hours after infection. Results provided novel information regarding the early functional pathways and gene network that orchestrate innate immune responses to S. agalactiae infection. This knowledge could contribute to new strategies to enhance resistance to this disease, such as genomic selection. MenosThis study aimed to evaluate the transcriptional changes occurring in isolated perfused mammary alveolar tissue in response to inoculation with S. agalactiae and to identify the most affected biological functions and pathways after 3 h. Four udders taken at slaughter from cows with healthy mammary gland were perfused ex situ with warmed and gassed Tyrode's solution. Mammary alveolar tissue samples were taken from the left fore and rear quarters (IQ-inoculated quarters) before inoculation (hour 0) and at 3 h post inoculation (hpi) and at the same times from control right fore and rear quarters (not inoculated: NIQ). A total of 1756 differentially expressed genes (DEGs) were identified between IQ and NIQ at 3 hpi using edgeR package. Within this set of DEGs, 952 were up regulated and mainly involved with innate immune response and inflammatory response, e.g., CD14, CCL5, TLR2, IL-8, SAA3, as well as in transcriptional regulation such as FOS, STAT3 and NFKBIA. Genes down-regulated (804) included those involved with lipid synthesis e.g., APOC2, SCD, FABP3 and FABP4. The most affected pathways were chemokine signaling, Wnt signaling and complement and coagulation cascades, which likely reflects the early stage response of mammary tissue to S. agalactiae infection. No significant gene expression changes were detected by RNA-Seq in the others contrasts. Real time-PCR confirmed the increase in mRNA abundance of immune-related genes: TLR2, TLR4, IL-1β, and IL-10 at 3 hpi between IQ a... Mostrar Tudo |
|
Palavras-Chave: |
Mammary gland perfused; RNA-Seq. |
|
Thesaurus Nal: |
Dairy cattle; Immune response; Mastitis. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 02979naa a2200337 a 4500
001 2117822
005 2024-02-06
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1017/s0022029919000451$2DOI
100 1 $aWELLER, M. M. D. C. A.
245 $aIsolated perfused udder model for transcriptome analysis in response to Streptococcus agalactiae.$h[electronic resource]
260 $c2019
520 $aThis study aimed to evaluate the transcriptional changes occurring in isolated perfused mammary alveolar tissue in response to inoculation with S. agalactiae and to identify the most affected biological functions and pathways after 3 h. Four udders taken at slaughter from cows with healthy mammary gland were perfused ex situ with warmed and gassed Tyrode's solution. Mammary alveolar tissue samples were taken from the left fore and rear quarters (IQ-inoculated quarters) before inoculation (hour 0) and at 3 h post inoculation (hpi) and at the same times from control right fore and rear quarters (not inoculated: NIQ). A total of 1756 differentially expressed genes (DEGs) were identified between IQ and NIQ at 3 hpi using edgeR package. Within this set of DEGs, 952 were up regulated and mainly involved with innate immune response and inflammatory response, e.g., CD14, CCL5, TLR2, IL-8, SAA3, as well as in transcriptional regulation such as FOS, STAT3 and NFKBIA. Genes down-regulated (804) included those involved with lipid synthesis e.g., APOC2, SCD, FABP3 and FABP4. The most affected pathways were chemokine signaling, Wnt signaling and complement and coagulation cascades, which likely reflects the early stage response of mammary tissue to S. agalactiae infection. No significant gene expression changes were detected by RNA-Seq in the others contrasts. Real time-PCR confirmed the increase in mRNA abundance of immune-related genes: TLR2, TLR4, IL-1β, and IL-10 at 3 hpi between IQ and NIQ. The expression profiles of Casp1 and Bax for any contrasts were unaffected whereas Bcl2 was increased in IQ, which suggests no induction of apoptosis during the first hours after infection. Results provided novel information regarding the early functional pathways and gene network that orchestrate innate immune responses to S. agalactiae infection. This knowledge could contribute to new strategies to enhance resistance to this disease, such as genomic selection.
650 $aDairy cattle
650 $aImmune response
650 $aMastitis
653 $aMammary gland perfused
653 $aRNA-Seq
700 1 $aFONSECA, I.
700 1 $aSBARDELLA, A. P.
700 1 $aPINTO, I. S. B.
700 1 $aVICCINI, L. F.
700 1 $aBRANDAO, H. de M.
700 1 $aGERN, J. C.
700 1 $aCARVALHO, W. A.
700 1 $aGUIMARÃES, A. S.
700 1 $aBRITO, M. A. V. P.
700 1 $aMUNARI, D. P.
700 1 $aSILVA, M. V. G. B.
700 1 $aMARTINS, M. F.
773 $tJournal of Dairy Research$gv. 86, n. 3, p. 307-314, 2019.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 8 | |
| 6. |  | ROGGIA, S.; GUEDES, J. V. C.; KUSS-ROGGIA, R. C. R.; VASCONCELOS, G. J. N. de; FERREIRA, D. N. M.; DELALIBERA JUNIOR, I. Ácaros predadores e o fungo Neozygites floridana associados a tetraniquídeos em soja no Rio Grande do Sul. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 44, n. 1, p. 107-110, jan. 2009. Notas Científicas. Título em inglês: Predatory mites and the fungus Neozygites floridana associated with spider mites on soybean, in Rio Grande do Sul, Brazil.| Biblioteca(s): Embrapa Unidades Centrais. |
|    |
| 7. | | ROGGIA, S.; GUEDES, J. V. C.; KUSS-ROGGIA, R.C.R.; VASCONCELOS, G. J. N. de; FERREIRA, D. N. M.; DELALIBERA JUNIOR, I. Ácaros predadores e o fungo Neozygites floridana associados a tetraniquídeos em soja no Rio Grande do Sul. Pesquisa Agropecuária Brasileira, Brasília, v.44, n. 1, p. 107-110, jan. 2009.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 8. | | PENA, M. R.; SILVA, N. M. da; MORAIS, E. G. F. de; NORONHA, A. C. da S.; VASCONCELOS, G. J. N. de; RODRIGUES, J. C. V.; LEMOS, W. de P. Citros. In: SILVA, N. M. da; ADAIME, R.; ZUCCHI, R. A. (Ed.). Pragas agrícolas e florestais na Amazônia. Brasília, DF: Embrapa, 2016. Pt. 1, cap. 6, p. 107-135.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Roraima. |
|  |
| Registros recuperados : 8 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
