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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
01/11/2019 |
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Data da última atualização: |
01/11/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
HAACH, V.; GAVA, D.; CANTAO, M. E.; FRANCO, A. C. |
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Afiliação: |
VANESSA HAACH, UFRGS; DANIELLE GAVA, CNPSA; MAURICIO EGIDIO CANTAO, CNPSA; ANA CLÁUDIA FRANCO, UFRGS. |
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Título: |
One-step multiplex RT-qPCR for the detection and subtyping of influenza A virus in swine in Brazil. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Journal of Virological Methods, v. 269, p. 43-48, 2019. |
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DOI: |
10.1016/j.jviromet.2019.04.005 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Pandemic H1N1, human-like H1N2 and H3N2 influenza A (IAV) viruses are co-circulating in swine herds in Brazil. The genetic analysis of the Brazilian IAVs has shown that they are genetically distinct from viruses found in swine in other countries; therefore, an update of the diagnostic assays for IAV detection and subtyping is needed. This study describes the development and validation of a TaqMan based ? one-step multiplex RT-qPCR to discriminate the hemagglutinin and neuraminidase genes of the three major IAV ubtypes circulating in pigs in Brazil. The RT-qPCR assays presented 100% (95.7?100, CI 95%) of diagnostic sensitivity in the analysis of 85 IAVs, previously characterized by sequencing. The limits of detection ranged from 5.09 × 101 to 5.09 × 103 viral RNA copies/μL. For the analytical specificity, 73 pig samples collected during 2017 and 2018 were analyzed, resulting in the identification of the subtype in 74.0% (62.9?82.7, CI 95%) of samples. From these, 46.3% were H3N2, 33.3% were H1N1, 11.1% were H1N2 and 3.7% were HxN1. Mixed viral infections (3.7%) and reassortant viruses (1.9%) were also detected by the test. This multiplex RT-qPCR assay provides a fast and specific diagnostic tool for identification of different subtypes and lineages of IAV in pigs, contributing to the monitoring of influenza in swine. |
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Palavras-Chave: |
Diagnóstico rápido; Multiplex RT-qPCR; Rapid diagnosis; RT-qPCR multiplex; Subtipagem; Subtyping; Vírus da gripe A. |
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Thesagro: |
Suíno. |
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Thesaurus Nal: |
Influenza A virus; Swine. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02191naa a2200289 a 4500
001 2113827
005 2019-11-01
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1016/j.jviromet.2019.04.005$2DOI
100 1 $aHAACH, V.
245 $aOne-step multiplex RT-qPCR for the detection and subtyping of influenza A virus in swine in Brazil.$h[electronic resource]
260 $c2019
520 $aAbstract: Pandemic H1N1, human-like H1N2 and H3N2 influenza A (IAV) viruses are co-circulating in swine herds in Brazil. The genetic analysis of the Brazilian IAVs has shown that they are genetically distinct from viruses found in swine in other countries; therefore, an update of the diagnostic assays for IAV detection and subtyping is needed. This study describes the development and validation of a TaqMan based ? one-step multiplex RT-qPCR to discriminate the hemagglutinin and neuraminidase genes of the three major IAV ubtypes circulating in pigs in Brazil. The RT-qPCR assays presented 100% (95.7?100, CI 95%) of diagnostic sensitivity in the analysis of 85 IAVs, previously characterized by sequencing. The limits of detection ranged from 5.09 × 101 to 5.09 × 103 viral RNA copies/μL. For the analytical specificity, 73 pig samples collected during 2017 and 2018 were analyzed, resulting in the identification of the subtype in 74.0% (62.9?82.7, CI 95%) of samples. From these, 46.3% were H3N2, 33.3% were H1N1, 11.1% were H1N2 and 3.7% were HxN1. Mixed viral infections (3.7%) and reassortant viruses (1.9%) were also detected by the test. This multiplex RT-qPCR assay provides a fast and specific diagnostic tool for identification of different subtypes and lineages of IAV in pigs, contributing to the monitoring of influenza in swine.
650 $aInfluenza A virus
650 $aSwine
650 $aSuíno
653 $aDiagnóstico rápido
653 $aMultiplex RT-qPCR
653 $aRapid diagnosis
653 $aRT-qPCR multiplex
653 $aSubtipagem
653 $aSubtyping
653 $aVírus da gripe A
700 1 $aGAVA, D.
700 1 $aCANTAO, M. E.
700 1 $aFRANCO, A. C.
773 $tJournal of Virological Methods$gv. 269, p. 43-48, 2019.
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