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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
23/09/2019 |
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Data da última atualização: |
23/09/2019 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
GREGORINE, L. F.; MONCLARO, A. V.; SILVA, C. de O. G.; RODRIGUES, K. B.; PEIXOTO, J. S. G.; ABDELNUR, P. V.; FAVARO, L. C. de L. |
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Afiliação: |
Lucas F. Gregorine; Antonielle V. Monclaro; Caio de Oliveira Gorgulho Silva, Consultor da Embrapa Agroenergia; Kelly B. Rodrigues; Jéssica S. G. Peixoto, UnB; PATRICIA VERARDI ABDELNUR, CNPAE; LEIA CECILIA DE LIMA FAVARO, CNPAE. |
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Título: |
Heterologous expression of a new lytic polysaccharide monooxygenase from Hahella ganghwensis and their functional characterization. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
In: SIMPÓSIO NACIONAL DE BIOPROCESSOS, 22.; SIMPÓSIO DE HIDRÓLISE ENZIMÁTICA DE BIOMASSA, 13., 2019, Uberlandia, MG. [Anais ...]. São Paulo: Associação Brasileira de Engenharia Química, 2019. |
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Idioma: |
Inglês |
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Conteúdo: |
The powerful class of oxidative enzymes, lytic polysaccharide monooxygenases (LPMOs) - also named Auxiliary Activity (AA) - are able to oxidize recalcitrant polysaccharides on lignocellulosic biomass. In this work, we successfully expressed three catalytic domains from bacterial LPMOs in the yeast Komagataella phaffii: domain MdAA10.1-SD (from Moritella dasanensis), domain VmAA10.2-SD (from Verrucosispora maris), and domain HgAA10.1-SD (from Hahella ganghwensis). Heterologous expression was analyzed by SDS-PAGE, Western-Blot, and Dot-Blot, while functional activity of these proteins was investigated by a combination of mass spectrometric and chromatographic methods. The recombinant LPMO catalytic domain HgAA10.1-SD from H. ganghwensis, a C1-oxidizer, was able to promote an oxidative cleavage of phosphoric-acid swollen cellulose (PASC) substrate in the presence of ascorbic acid as an electron donor, showing its potential for cellulose depolymerization. |
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Palavras-Chave: |
Chromatographic; Spectrometric. |
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Categoria do assunto: |
-- |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/1112393/1/107193786998fieldsubmissionfulltextfile1.pdf
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Marc: |
LEADER 01787nam a2200205 a 4500
001 2112393
005 2019-09-23
008 2019 bl uuuu u00u1 u #d
100 1 $aGREGORINE, L. F.
245 $aHeterologous expression of a new lytic polysaccharide monooxygenase from Hahella ganghwensis and their functional characterization.$h[electronic resource]
260 $aIn: SIMPÓSIO NACIONAL DE BIOPROCESSOS, 22.; SIMPÓSIO DE HIDRÓLISE ENZIMÁTICA DE BIOMASSA, 13., 2019, Uberlandia, MG. [Anais ...]. São Paulo: Associação Brasileira de Engenharia Química$c2019
520 $aThe powerful class of oxidative enzymes, lytic polysaccharide monooxygenases (LPMOs) - also named Auxiliary Activity (AA) - are able to oxidize recalcitrant polysaccharides on lignocellulosic biomass. In this work, we successfully expressed three catalytic domains from bacterial LPMOs in the yeast Komagataella phaffii: domain MdAA10.1-SD (from Moritella dasanensis), domain VmAA10.2-SD (from Verrucosispora maris), and domain HgAA10.1-SD (from Hahella ganghwensis). Heterologous expression was analyzed by SDS-PAGE, Western-Blot, and Dot-Blot, while functional activity of these proteins was investigated by a combination of mass spectrometric and chromatographic methods. The recombinant LPMO catalytic domain HgAA10.1-SD from H. ganghwensis, a C1-oxidizer, was able to promote an oxidative cleavage of phosphoric-acid swollen cellulose (PASC) substrate in the presence of ascorbic acid as an electron donor, showing its potential for cellulose depolymerization.
653 $aChromatographic
653 $aSpectrometric
700 1 $aMONCLARO, A. V.
700 1 $aSILVA, C. de O. G.
700 1 $aRODRIGUES, K. B.
700 1 $aPEIXOTO, J. S. G.
700 1 $aABDELNUR, P. V.
700 1 $aFAVARO, L. C. de L.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
03/10/2016 |
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Data da última atualização: |
06/03/2017 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
MAGRO, M.; RUFATO, L.; SANDER, G. F.; SANDOS, A. M. dos; RUFATO, A. de R.; KRETZSCHMAR, A. A.; ROBINSON, T. L. |
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Afiliação: |
Maicon Magro; leo Rufato; Guilherme F. Sander; Aline M dos Santos; ANDREA DE ROSSI RUFATO, CNPUV; Aike A. Kretzschmar; Terence L. Robinson. |
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Título: |
Maturation behavior of Maxi Gala grafetd on two rootstocks by no Destructive Method. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
In: ORCHAD SYSTEMS SYMPOSIUM, 11., 2016. Integrating Canopy, Rootstookhand Environment Physiology in Orchard Systems: Anais... .Bologna, 28/08 a 2/09 de 2016. p. 107. |
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Idioma: |
Inglês |
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Conteúdo: |
The evaluation of the maturation in apple orchards is checked using destructive methods, sampling fruits and analyzing them in the laboratory, making the process slow and expensive. The use of not destructive method to determine fruit maturation in the orchard could accelerate delivery of results and help in determining harvest time, because non-destructive data would allow to verify the maturation on different blocks in the orchard. The aim of this work was to chart fruit maturation in 'Maxi Gala' grafted on two different rootstocks, using destructive and not destructive methods. The non-destructive method used was the portable DA-Meter. The trial was realized at Vacaria, southern Brazillocated 28,44 S and 50,85 W. The samples were harvested on two orchards during the seasons 2014/15 and 2015/16, during six weeks before harvest from January until the second week of February. The sampling was realized in five different points of the orchard, on rootstocks M.9 or Marubakaido with M.9 interstem. Ten-apple samples were collected weekly in each point in the orchard and then evaluated by destructive method (flesh firmness, starch degradation, total soluble solids and acidity) and the not destructive method (DA-Meter). For both seasons, the evolution of the fruit maturation of Maxi Gala showed a similar progression for both rootstocks. The non-destructive method correlated well with the traditional destructive methods, making it a tool for more practical and easy determination of the harvest date. MenosThe evaluation of the maturation in apple orchards is checked using destructive methods, sampling fruits and analyzing them in the laboratory, making the process slow and expensive. The use of not destructive method to determine fruit maturation in the orchard could accelerate delivery of results and help in determining harvest time, because non-destructive data would allow to verify the maturation on different blocks in the orchard. The aim of this work was to chart fruit maturation in 'Maxi Gala' grafted on two different rootstocks, using destructive and not destructive methods. The non-destructive method used was the portable DA-Meter. The trial was realized at Vacaria, southern Brazillocated 28,44 S and 50,85 W. The samples were harvested on two orchards during the seasons 2014/15 and 2015/16, during six weeks before harvest from January until the second week of February. The sampling was realized in five different points of the orchard, on rootstocks M.9 or Marubakaido with M.9 interstem. Ten-apple samples were collected weekly in each point in the orchard and then evaluated by destructive method (flesh firmness, starch degradation, total soluble solids and acidity) and the not destructive method (DA-Meter). For both seasons, the evolution of the fruit maturation of Maxi Gala showed a similar progression for both rootstocks. The non-destructive method correlated well with the traditional destructive methods, making it a tool for more practical and easy determination of ... Mostrar Tudo |
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Palavras-Chave: |
Apple orchards; DA-Meter; M 9; Macieira; Marubakaido; Pomares de maçã. |
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Thesagro: |
Maca. |
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Thesaurus NAL: |
fruit quality. |
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Categoria do assunto: |
A Sistemas de Cultivo |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/1054009/1/AndreaDeR.Rufato107.pdf
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Marc: |
LEADER 02399nam a2200277 a 4500
001 2054009
005 2017-03-06
008 2016 bl uuuu u00u1 u #d
100 1 $aMAGRO, M.
245 $aMaturation behavior of Maxi Gala grafetd on two rootstocks by no Destructive Method.$h[electronic resource]
260 $aIn: ORCHAD SYSTEMS SYMPOSIUM, 11., 2016. Integrating Canopy, Rootstookhand Environment Physiology in Orchard Systems: Anais... .Bologna, 28/08 a 2/09 de 2016. p. 107.$c2016
520 $aThe evaluation of the maturation in apple orchards is checked using destructive methods, sampling fruits and analyzing them in the laboratory, making the process slow and expensive. The use of not destructive method to determine fruit maturation in the orchard could accelerate delivery of results and help in determining harvest time, because non-destructive data would allow to verify the maturation on different blocks in the orchard. The aim of this work was to chart fruit maturation in 'Maxi Gala' grafted on two different rootstocks, using destructive and not destructive methods. The non-destructive method used was the portable DA-Meter. The trial was realized at Vacaria, southern Brazillocated 28,44 S and 50,85 W. The samples were harvested on two orchards during the seasons 2014/15 and 2015/16, during six weeks before harvest from January until the second week of February. The sampling was realized in five different points of the orchard, on rootstocks M.9 or Marubakaido with M.9 interstem. Ten-apple samples were collected weekly in each point in the orchard and then evaluated by destructive method (flesh firmness, starch degradation, total soluble solids and acidity) and the not destructive method (DA-Meter). For both seasons, the evolution of the fruit maturation of Maxi Gala showed a similar progression for both rootstocks. The non-destructive method correlated well with the traditional destructive methods, making it a tool for more practical and easy determination of the harvest date.
650 $afruit quality
650 $aMaca
653 $aApple orchards
653 $aDA-Meter
653 $aM 9
653 $aMacieira
653 $aMarubakaido
653 $aPomares de maçã
700 1 $aRUFATO, L.
700 1 $aSANDER, G. F.
700 1 $aSANDOS, A. M. dos
700 1 $aRUFATO, A. de R.
700 1 $aKRETZSCHMAR, A. A.
700 1 $aROBINSON, T. L.
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Embrapa Uva e Vinho (CNPUV) |
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