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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
13/08/2019 |
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Data da última atualização: |
20/10/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, H. A. P. da; CAETANO, V. S.; PESSOA, D. D. V.; PACHECO, R. S.; ARAUJO, J. L. S. de. |
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Afiliação: |
Helder Anderson Pinto da Silva, UFRJ; Vanessa Santana Caetano, UFRRJ; Daniella Duarte Villarinho Pessoa, UFRJ; UFRRJ; JEAN LUIZ SIMOES DE ARAUJO, CNPAB. |
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Título: |
Molecular and biochemical changes of aging-induced nodules senescence in common bean. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Symbiosis, v. 79, p. 33-48, 2019. |
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DOI: |
https://doi.org/10.1007/s13199-019-00618-2 |
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Idioma: |
Inglês |
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Conteúdo: |
In the common bean (Phaseolus vulgaris L.), premature nodule senescence affects biological nitrogen fixation (BNF), reducing crop yield. Here, we investigated the molecular and biochemical changes in common bean nodules undergoing aging senescence. The experiments were carried out with three common bean genotypes and two nodule groups: senescent and non-senescent nodules. The expression patterns of 12 genes were assessed using RT-qPCR. In addition, global activities of ascorbate peroxidase (APX) and catalase (CAT), lipid peroxidation and total sugar content of nodules were also determined. Our results show that PvLb gene expression was down-regulated and PvGS(n-1) and PvUriII, genes involved in N metabolism, also decreased in senescent nodules. Transcripts related to the ethylene, abscisic acid and cytokinin phytohormones were up-regulated upon nodule senescence. Interestingly, PvSnakin-2 (SNAKIN-like cysteine rich protein), a gene related to plant-pathogen interaction, was induced in senescent nodules. The biochemical assays showed that in nodules undergoing senescence, APX activity was altered in only one genotype but CAT activity decreased in all common bean genotypes. Lipid peroxidation increased but total sugar content was not altered in nodules upon senescence. All these findings suggest that a delayed plant-response to Rhizobium infection is activated in common bean nodules undergoing aging senescence and this mechanism seems to be regulated by phytohormones and cell signaling as well, whereas the antioxidant defenses mediated by CAT are repressed, suggesting oxidative damage in nodules upon senescence. Our results offer insights into understanding nodule senescence metabolism and provide potential senescence markers for common bean nodules. MenosIn the common bean (Phaseolus vulgaris L.), premature nodule senescence affects biological nitrogen fixation (BNF), reducing crop yield. Here, we investigated the molecular and biochemical changes in common bean nodules undergoing aging senescence. The experiments were carried out with three common bean genotypes and two nodule groups: senescent and non-senescent nodules. The expression patterns of 12 genes were assessed using RT-qPCR. In addition, global activities of ascorbate peroxidase (APX) and catalase (CAT), lipid peroxidation and total sugar content of nodules were also determined. Our results show that PvLb gene expression was down-regulated and PvGS(n-1) and PvUriII, genes involved in N metabolism, also decreased in senescent nodules. Transcripts related to the ethylene, abscisic acid and cytokinin phytohormones were up-regulated upon nodule senescence. Interestingly, PvSnakin-2 (SNAKIN-like cysteine rich protein), a gene related to plant-pathogen interaction, was induced in senescent nodules. The biochemical assays showed that in nodules undergoing senescence, APX activity was altered in only one genotype but CAT activity decreased in all common bean genotypes. Lipid peroxidation increased but total sugar content was not altered in nodules upon senescence. All these findings suggest that a delayed plant-response to Rhizobium infection is activated in common bean nodules undergoing aging senescence and this mechanism seems to be regulated by phytohormones and cell ... Mostrar Tudo |
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Palavras-Chave: |
Biological nitrogen fixation; Nodule senescence; Root nodule RT-qPCR. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02458naa a2200217 a 4500
001 2111331
005 2021-10-20
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s13199-019-00618-2$2DOI
100 1 $aSILVA, H. A. P. da
245 $aMolecular and biochemical changes of aging-induced nodules senescence in common bean.$h[electronic resource]
260 $c2019
520 $aIn the common bean (Phaseolus vulgaris L.), premature nodule senescence affects biological nitrogen fixation (BNF), reducing crop yield. Here, we investigated the molecular and biochemical changes in common bean nodules undergoing aging senescence. The experiments were carried out with three common bean genotypes and two nodule groups: senescent and non-senescent nodules. The expression patterns of 12 genes were assessed using RT-qPCR. In addition, global activities of ascorbate peroxidase (APX) and catalase (CAT), lipid peroxidation and total sugar content of nodules were also determined. Our results show that PvLb gene expression was down-regulated and PvGS(n-1) and PvUriII, genes involved in N metabolism, also decreased in senescent nodules. Transcripts related to the ethylene, abscisic acid and cytokinin phytohormones were up-regulated upon nodule senescence. Interestingly, PvSnakin-2 (SNAKIN-like cysteine rich protein), a gene related to plant-pathogen interaction, was induced in senescent nodules. The biochemical assays showed that in nodules undergoing senescence, APX activity was altered in only one genotype but CAT activity decreased in all common bean genotypes. Lipid peroxidation increased but total sugar content was not altered in nodules upon senescence. All these findings suggest that a delayed plant-response to Rhizobium infection is activated in common bean nodules undergoing aging senescence and this mechanism seems to be regulated by phytohormones and cell signaling as well, whereas the antioxidant defenses mediated by CAT are repressed, suggesting oxidative damage in nodules upon senescence. Our results offer insights into understanding nodule senescence metabolism and provide potential senescence markers for common bean nodules.
653 $aBiological nitrogen fixation
653 $aNodule senescence
653 $aRoot nodule RT-qPCR
700 1 $aCAETANO, V. S.
700 1 $aPESSOA, D. D. V.
700 1 $aPACHECO, R. S.
700 1 $aARAUJO, J. L. S. de
773 $tSymbiosis$gv. 79, p. 33-48, 2019.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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| Registros recuperados : 36 | |
| 4. |  | BARBOSA, W. C.; NAZARE, R. F. R. de; NAGATA, I. Estudos físicos e químicos dos frutos: bacuri (Platonia insignis), capuaçu (Theobroma grandiflorum) e muruci (Byrsonima crassifolia). In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 5., 1979, Pelotas, RS. Anais... Pelotas: SBF, 1979. v. 2, p. 797-808.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 6. | | BARBOSA, W. C.; NAZARE, R. F. R. de; HASHIMOTO, K. Estudo bromatológico e tecnológico da graviola e do taperebá. In: ENCONTRO DE PROFISSIONAIS DE QUÍMICA DA AMAZÔNIA, 1., 1980, Belém, PA; ENCONTRO DE PROFISSIONAIS DA QUÍMICA DA AMAZÔNIA, 2., 1981, São Luís. Anais. Belém, PA: Conselho Regional de Química da 6. Região, [1982?]. p. 307-318.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 11. | | NAZARÉ, R. F. R. de; MELO, C. F. M. de; BARBOSA, W. C. Potencial de frutas e outros produtos alimentícios de origem vegetal, na região Norte. In: SIMPÓSIO DO TRÓPICO ÚMIDO, 1., 1984, Belém, PA. Resumos. Belém, PA: EMBRAPA-CPATU, 1984. p. 260. (EMBRAPA-CPATU. Documentos, 31).| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 12. | | NAZARE, R. F. R. de; MELO, C. F. M. de; BARBOSA, W. C. Potencial de frutas e outros produtos alimentícios de origem vegetal, na região Norte. In: SIMPÓSIO DO TRÓPICO ÚMIDO, 1., 1984, Belém, PA. Anais. Belém, PA: EMBRAPA-CPATU, 1986. v. 4, p. 211-221. (EMBRAPA-CPATU. Documentos, 36). v.4 Culturas perenes.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 14. | | BARBOSA, W. C.; MELO, C. F. M. de; NAZARÉ, R. F. R. de. Produtos alimentícios de origem vegetal elaborados na EMBRAPA-CPATU. In: SIMPÓSIO DO TRÓPICO ÚMIDO, 1., 1984, Belém, PA. Resumos. Belém, PA: EMBRAPA-CPATU, 1984. p. 237. (EMBRAPA-CPATU. Documentos, 31).| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 15. | | BARBOSA, W. C.; MELO, C. F. M. de; NAZARÉ, R. F. R. de. Produtos alimentícios de origem vegetal elaborados na EMBRAPA-CPATU. In: SIMPÓSIO DO TRÓPICO ÚMIDO, 1., 1984, Belém, PA. Anais. Belém, PA: EMBRAPA-CPATU, 1986. v. 4, p. 65-79. (EMBRAPA-CPATU. Documentos, 36). v.4 Culturas perenes.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 17. | | CARDOSO, R. C. D.; CARVALHO, J. E. U. de; BARBOSA, W. C. Caracterização físico-química das frações polpa aderida às sementes e segmentos partenocárpicos do Bacuri (Platonia insignis Mart.). In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 17., 2002, Belém, PA. Anais. Belém: SBF, 2002. 4f. Publicado também: FRAZÃO, D. A. C.; HOMMA, A. K. O; VIÉGAS, I. de J. M. (Ed.). Contribuição ao desenvolvimento da fruticultura na Amazônia. Belém, PA: Embrapa Amazônia Oriental, 2006. p. 143-149.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 18. | | CARDOSO, R. C. D.; CARVALHO, J. E. U. de; BARBOSA, W. C. Caracterização física e físico-química da marirana (Couepia subcordata Benth.). In: CONGRESSO NACIONAL DE BOTÂNICA, 54.; REUNIÃO AMAZÔNICA DE BOTÂNICA, 3., 2003, Belém, PA. Botânica: desafios da botânica brasileira no novo milênio: inventário, sistematização, conservação e uso da diversidade vegetal: resumos. Belém, PA: Sociedade Botânica do Brasil: UFRA: Museu Paraense Emílio Goeldi: Embrapa Amazônia Oriental, 2003. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 20. | | FALESI, I. C.; KATO, O. R.; BELFORT, A. J. L.; BARBOSA, W. C. Influência da cor dos frutos do urucuzeiro nos teores de bixina nas sementes. In: REUNIÃO TÉCNICO-CIENTÍFICA SOBRE O MELHORAMENTO GENÉTICO DO URUCUZEIRO, 1., 1991, Belém, PA. Anais. Belém, PA: EMBRAPA-CPATU, 1992. p. 27-31. (EMBRAPA-CPATU. Documentos, 69).| Biblioteca(s): Embrapa Amazônia Oriental. |
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| Registros recuperados : 36 | |
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