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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
15/03/2019 |
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Data da última atualização: |
18/11/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
COSTA, P. P. K. G.; SALUM, T. F. C.; PACHECO, T. F.; BRAGA, S. C.; ALMEIDA, J. R. M. de; GONCALVES, S. B.; DAMASO, M. C. T.; RODRIGUES, C. M. |
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Afiliação: |
PATRICIA PINTO KALIL G COSTA, CNPAE; THAIS FABIANA CHAN SALUM, CNPAE; THALYTA FRAGA PACHECO, CNPAE; Samira C. Braga, UnB; JOAO RICARDO MOREIRA DE ALMEIDA, CNPAE; SILVIA BELEM GONCALVES, CNPAE; MONICA CARAMEZ TRICHES DAMASO, CNPAE; CLENILSON MARTINS RODRIGUES, CNPAE. |
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Título: |
Development and validation of HILIC-UHPLC-ELSD methods fordetermination of sugar alcohols stereoisomers and its application forbioconversion processes of crude glycerin. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Journal of Chromatography A, v. 1589, p. 56-64, 2019. |
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Idioma: |
Inglês |
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Conteúdo: |
The recent increase in the production of crude glycerin through the manufacture of biodiesel has imputeda commercial issue, the excess of this raw material in the market and its constant devaluation, whichresulted in the need for new technologies for its use. Crude glycerin can be used in biotechnologicalprocesses for the production of high value-added compounds. This study presents novel, simple and fastmethods based on ultra-high performance liquid chromatography (UHPLC) using evaporative light scat-tering detection (ELSD) for simultaneous analysis of ten sugar alcohols with a hydrophilic interactionchromatography (HILIC) column. The selected compounds and their possible stereoisomers have majorcommercial importance and they can be obtained by biotechnological routes. Under optimized condi-tions, threitol, erythritol, adonitol, xylitol, arabitol, iditol, sorbitol, mannitol, dulcitol and volemitol canbe analyzed simultaneously within 15.0 min. The use of different column temperatures was a key param-eter to reach the selectivity during the separation of some stereoisomers. Regression equations revealeda good linear relationship (R > 0.995) over the range from 50.0 to 800.0 ng. Limits of detection (LOD) andquantification (LOQ) ranged from 30.0 to 45.0 ng and 50.0?75.0 ng, respectively. The HILIC-UHPLC-ELSDmethods showed good precision with low coefficient of variation (CV%) for the intra- and inter-assaysexperiments (≤ 5.1%) and high repeatability in terms of retention times for each analyte (≤ 0.5%). Theaccuracy was confirmed with an average recovery ranging from 92.3 to 107.3%. The developed methodsemploy an analytical technique more accessible and suitable for routine analyzes and have shown to besuitable for simultaneous analysis of sugar alcohols present in crude bioconverted glycerin samples usingdifferent classes of microorganisms. MenosThe recent increase in the production of crude glycerin through the manufacture of biodiesel has imputeda commercial issue, the excess of this raw material in the market and its constant devaluation, whichresulted in the need for new technologies for its use. Crude glycerin can be used in biotechnologicalprocesses for the production of high value-added compounds. This study presents novel, simple and fastmethods based on ultra-high performance liquid chromatography (UHPLC) using evaporative light scat-tering detection (ELSD) for simultaneous analysis of ten sugar alcohols with a hydrophilic interactionchromatography (HILIC) column. The selected compounds and their possible stereoisomers have majorcommercial importance and they can be obtained by biotechnological routes. Under optimized condi-tions, threitol, erythritol, adonitol, xylitol, arabitol, iditol, sorbitol, mannitol, dulcitol and volemitol canbe analyzed simultaneously within 15.0 min. The use of different column temperatures was a key param-eter to reach the selectivity during the separation of some stereoisomers. Regression equations revealeda good linear relationship (R > 0.995) over the range from 50.0 to 800.0 ng. Limits of detection (LOD) andquantification (LOQ) ranged from 30.0 to 45.0 ng and 50.0?75.0 ng, respectively. The HILIC-UHPLC-ELSDmethods showed good precision with low coefficient of variation (CV%) for the intra- and inter-assaysexperiments (≤ 5.1%) and high repeatability in terms of retention... Mostrar Tudo |
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Palavras-Chave: |
Crude glycerin. |
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Thesaurus Nal: |
Biotechnology; Sugar alcohols. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02678naa a2200241 a 4500
001 2107126
005 2019-11-18
008 2019 bl uuuu u00u1 u #d
100 1 $aCOSTA, P. P. K. G.
245 $aDevelopment and validation of HILIC-UHPLC-ELSD methods fordetermination of sugar alcohols stereoisomers and its application forbioconversion processes of crude glycerin.$h[electronic resource]
260 $c2019
520 $aThe recent increase in the production of crude glycerin through the manufacture of biodiesel has imputeda commercial issue, the excess of this raw material in the market and its constant devaluation, whichresulted in the need for new technologies for its use. Crude glycerin can be used in biotechnologicalprocesses for the production of high value-added compounds. This study presents novel, simple and fastmethods based on ultra-high performance liquid chromatography (UHPLC) using evaporative light scat-tering detection (ELSD) for simultaneous analysis of ten sugar alcohols with a hydrophilic interactionchromatography (HILIC) column. The selected compounds and their possible stereoisomers have majorcommercial importance and they can be obtained by biotechnological routes. Under optimized condi-tions, threitol, erythritol, adonitol, xylitol, arabitol, iditol, sorbitol, mannitol, dulcitol and volemitol canbe analyzed simultaneously within 15.0 min. The use of different column temperatures was a key param-eter to reach the selectivity during the separation of some stereoisomers. Regression equations revealeda good linear relationship (R > 0.995) over the range from 50.0 to 800.0 ng. Limits of detection (LOD) andquantification (LOQ) ranged from 30.0 to 45.0 ng and 50.0?75.0 ng, respectively. The HILIC-UHPLC-ELSDmethods showed good precision with low coefficient of variation (CV%) for the intra- and inter-assaysexperiments (≤ 5.1%) and high repeatability in terms of retention times for each analyte (≤ 0.5%). Theaccuracy was confirmed with an average recovery ranging from 92.3 to 107.3%. The developed methodsemploy an analytical technique more accessible and suitable for routine analyzes and have shown to besuitable for simultaneous analysis of sugar alcohols present in crude bioconverted glycerin samples usingdifferent classes of microorganisms.
650 $aBiotechnology
650 $aSugar alcohols
653 $aCrude glycerin
700 1 $aSALUM, T. F. C.
700 1 $aPACHECO, T. F.
700 1 $aBRAGA, S. C.
700 1 $aALMEIDA, J. R. M. de
700 1 $aGONCALVES, S. B.
700 1 $aDAMASO, M. C. T.
700 1 $aRODRIGUES, C. M.
773 $tJournal of Chromatography A$gv. 1589, p. 56-64, 2019.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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| Registros recuperados : 12 | |
| 1. |  | BERENGUER, E.; GARDNER, T.; FERREIRA, J. N.; ARAGÃO, L.; NALLY, R. M.; THOMSON, J.; VIEIRA, I.; BARLOW, J. Seeing the woods through the saplings: using wood density to assess post-disturbance recovery of human-modified tropical forests. In: ANNUAL MEETING OF THE ASSOCIATION FOR TROPICAL BIOLOGY AND CONSERVATION, 53., 2016, Montpellier. Tropical ecology and society: reconciling conservation and sustainable use of biodiversity: program & abstracts. [S.l.]: ATBC, 2016. p. 313.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 2. | | MOURA, N. G.; LEES, A. C.; ALEIXO, A.; BARLOW, J.; BERENGUER, E.; FERREIRA, J.; NALLY, R. M.; THOMSON, J. R.; GARDNER, T. A. Idiosyncratic responses of Amazonian birds to primary forest disturbance. Oecologia, v. 180, n. 3, p. 903-916, Mar. 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
|  |
| 3. | | BERENGUER, E.; GARDNER, T. A.; FERREIRA, J. N.; ARAGÃO, L. E. O. C.; NALLY, R. M.; THOMSON, J. R.; VIEIRA, I. C. G.; BARLOW, J. Seeing the woods through the saplings: Using wood density to assess the recovery of human-modified Amazonian forests. Journal of Ecology, v. 106, n. 6, p. 2190-2203, Nov. 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 4. | | LEAL, C. G.; POMPEU, P. S.; GARDNER, T. A.; LEITÃO, R. P.; HUGHES, R. M.; KAUFMANN, P. R.; ZUANON, J.; PAULA, F. R. de; FERRAZ, S. F. B.; THOMSON, J. R.; NALLY, R. M.; FERREIRA, J.; BARLOW, J. Multi-scale assessment of human-induced changes to Amazonian instream habitats. Landscape Ecology, v. 31, n. 8, p. 1725-1745, Oct. 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 5. | | SOLAR, R. R. de C.; BARLOW, J.; FERREIRA, J.; BERENGUER, E.; LEES, A. C.; THOMSON, J. R.; LOUZADA, J.; MAUES, M.; MOURA, N. G.; OLIVEIRA, V. H. F.; CHAUL, J. C. M.; SCHOEREDER, J. H.; VIEIRA, I. C. G.; NALLY, R. M.; GARDNER, T. A. How pervasive is biotic homogenization in human-modified tropical forest landscapes? Ecology Letters, v. 18, n. 10, p. 1108-1118, Oct. 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 6. | | LENNOX, G. D.; GARDNER, T. A.; THOMSON, J. R.; FERREIRA, J. N.; BERENGUER, E.; LEES, A. C.; NALLY, R. M.; ARAGÃO, L. E. O. C.; FERRAZ, S. F. B.; LOUZADA, J.; MOURA, N. G.; OLIVEIRA, V. H. F.; PARDINI, R.; SOLAR, R. R. C.; MELLO, F. Z. V. de; VIEIRA, I. C. G.; BARLOW, J. Second rate or a second chance? Assessing biomass and biodiversity recovery in regenerating Amazonian forests. Global Change Biology, v. 24, n. 12, p. 5680-5694, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 7. | | LEAL, C.; LEITÃO, R.; CAMPOS, V.; OLIVEIRA-JUNIOR, J. M.; SILVA, K.; BRITO, J.; JUEN, L.; POMPEU, P.; BARLOW, J.; FERREIRA, J. N.; ZUANON, J.; HAMADA, N.; HUGHES, R.; KAUFMANN, P.; ROSSETTI, F.; FERRAZ, S.; THOMPSON, J.; NALLY, R. M.; GARDNER, T. Small rivers, big impacts: environmental disturbances to aquatic biodiversity in Eastern Amazon. In: ANNUAL MEETING OF THE ASSOCIATION FOR TROPICAL BIOLOGY AND CONSERVATION, 53., 2016, Montpellier. Tropical ecology and society: reconciling conservation and sustainable use of biodiversity: program & abstracts. [S.l.]: ATBC, 2016. p. 187.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 8. | | LEAL, C. G.; BARLOW, J.; GARDNER, T. A.; HUGHES, R. M.; LEITÃO, R. P.; NALLY, R. M.; KAUFMANN, P. R.; FERRAZ, S. F. B.; ZUANON, J.; PAULA, F. R. de; FERREIRA, J. N.; THOMSON, J. R.; LENNOX, G. D.; DARY, E. P.; RÖPKE, C. P.; POMPEU, P. S. Is environmental legislation conserving tropical stream faunas? A large-scale assessment of local, riparian and catchment-scale influences on Amazonian fish. Journal of Applied Ecology, v. 55, n. 3, p. 1312-1326, May 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 9. | | THOMPSON, J.; FERREIRA, J. N.; ARAGÃO, L.; BARLOW, J.; BERENGUER, E.; BRANCALLION, P.; FERRAZ, S.; FONSECA, T.; GARDNER, T.; GARRETT, R.; OLIVEIRA, V. H. F.; LEES, A.; LENNOX, G.; LOUZADA, J.; NALLY, R. M.; MOURA, N.; NUNES, S.; PARRY, L.; SOLAR, R.; VIEIRA, I. Prioritizing forest protection, reforestation, and avoided disturbance in the eastern Amazon. In: ANNUAL MEETING OF THE ASSOCIATION FOR TROPICAL BIOLOGY AND CONSERVATION, 53., 2016, Montpellier. Tropical ecology and society: reconciling conservation and sustainable use of biodiversity: program & abstracts. [S.l.]: ATBC, 2016. p. 186.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 10. | | BARLOW, J.; LENNOX, G. D.; FERREIRA, J.; BERENGUER, E.; LEES, A. C.; NALLY, R. M.; THOMSON, J. R.; FERRAZ, S. F. de B.; LOUZADA, J.; OLIVEIRA, V. H. F.; PARRY, L.; SOLAR, R. R. de C.; VIEIRA, I. C. G.; ARAGÃO, L. E. O. C.; BEGOTTI, R. A.; BRAGA, R. F.; CARDOSO, T. M.; OLIVEIRA JUNIOR, R. C. de; SOUZA JUNIOR, C. M.; MOURA, N. G.; NUNES, S. S.; SIQUEIRA, J. V.; PARDINI, R.; SILVEIRA, J. M.; VAZ-DE-MELLO, F. Z.; VEIGA, R. C. S.; VENTURIERI, A.; GARDNER, T. A. Anthropogenic disturbance in tropical forests can double biodiversity loss from deforestation. Nature, v. 535, n. 7610, p. 144-147, July 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 11. | | LEAL, C. G.; LENNOX, G. D.; FERRAZ, S. F. B.; FERREIRA, J. N.; GARDNER, T. A.; THOMSON, J. R.; BERENGUER, E.; LEES, A. C.; HUGHES, R. M.; NALLY, R. M.; ARAGÃO, L. E. O. C.; BRITO, J. G. de; CASTELLO, L.; GARRETT, R. D.; HAMADA, N.; JUEN, L.; LEITÃO, R. P.; LOUZADA, J.; MORELLO, T. F.; MOURA, N. G.; NESSIMIAN, J. L.; OLIVEIRA-JUNIOR, J. M. B.; OLIVEIRA, V. H. F.; OLIVEIRA, V. C. de; PARRY, L.; POMPEU, P. S.; SOLAR, R. R. C.; ZUANON, J.; BARLOW, J. Integrated terrestrial-freshwater planning doubles conservation of tropical aquatic species. Science, v. 370, n. 6512, p. 117-121, Oct. 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 12. | | GARDNER, T. A.; FERREIRA, J.; BARLOW, J.; LEES, A. C.; PARRY, L.; VIEIRA, I. C. G.; BERENGUER, E.; ABRAMOVAY, R.; ALEIXO, A.; ANDRETTI, C.; ARAGAO, L. E. O. C.; ARAUJO, I.; AVILA, W. S. de; BARDGETT, R. D.; BATISTELLA, M.; BEGOTTI, R. A.; BELDINI, T.; BLAS, D. E. de; BRAGA, R. F.; BRAGA, D. de L.; BRITO, J. G. de; CAMARGO, P. B. de; SANTOS, F. C. dos; OLIVEIRA, V. C. de; CORDEIRO, A. C. N.; CARDOSO, T. M.; CARVALHO, D. R. de; CASTELANI, S. A.; CHAUL, J. C. M.; CERRI, C. E.; COSTA, F. de A.; COSTA, C. D. F. da; COUDEL, E.; COUTINHO, A. C.; CUNHA, D.; D'ANTONA, A.; DEZINCOURT, J.; DIAS-SILVA, K.; DURIGAN, M.; ESQUERDO, J. C. D. M.; FERES, J.; FERRAZ, S. F. de B.; FERREIRA, A. E. de M.; FIORINI, A. C.; SILVA, L. V. F. da; FRAZAO, F. S.; GARRETT, R.; GOMES, A. dos S.; GONCALVES, K. da S.; GUERRERO, J. B.; HAMADA, N.; HUGHES, R. M.; IGLIORI, D. C.; JESUS, E. da C.; JUEN, L.; MIÉRCIO JUNIOR; OLIVEIRA JUNIOR, J. M. B. de; OLIVEIRA JUNIOR, R. C. de; SOUZA JUNIOR, C.; KAUFMANN, P.; KORASAKI, V.; LEAL, C. G.; LEITAO, R.; LIMA, N.; ALMEIDA, M. de F. L.; LOURIVAL, R.; LOUZADA, J.; NALLY, R. M.; MARCHAND, S.; MAUES, M. M.; MOREIRA, F. M. S.; MORSELLO, C.; MOURA, N.; NESSIMIAN, J.; NUNES, S.; OLIVEIRA, V. H. F.; PARDINI, R.; PEREIRA, H. C.; POMPEU, P. S.; RIBAS, C. R.; ROSSETTI, F.; SCHMIDT, F. A.; SILVA, R. da; MARTINS DA SILVA, R. C. V.; SILVA, T. F. M. R. da; SILVEIRA, J.; SIQUEIRA, J. V.; CARVALHO, T. S. de; SOLAR, R. R. C.; TANCREDI, N. S. H.; THOMSON, J. R.; TORRES, P. C.; VAZ-DE-MELLO, F. Z.; VEIGA, R. C. S.; VENTURIERI, A.; VIANA, C.; WEINHOLD, D.; ZANETTI, R.; ZUANON, J. A social and ecological assessment of tropical land uses at multiple scales: the Sustainable Amazon Network. Philosophical Transactions of The Royal Society, serie B: Biological Sciences, v. 368, n. 1619, p. 1-11, Apr. 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Amazônia Oriental; Embrapa Territorial. |
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| Registros recuperados : 12 | |
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