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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
13/12/2018 |
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Data da última atualização: |
03/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RODRIGUES, J. P.; PETI, A. P. F.; FIGUEIRÓ, F. S.; ROCHA, I. S.; ACQUARO JUNIOR, V. R.; SILVA, T. G.; MELO, I. S. de; BEHLAU, F.; MORAES, L. A. B. |
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Afiliação: |
JULIA PEREIRA RODRIGUES, FFCLRP-USP; ANA PAULA FERRANTI PETI, FFCLRP-USP; FERNANDA SALES FIGUEIRO, FFCLRP-USP; IZADORA DE SOUZA ROCHA, FFCLRP-USP; VINICIUS RICARDO ACQUARO JUNIOR, FFCLRP-USP; TAMIRES GARCIA SILVA, Fundecitrus; ITAMAR SOARES DE MELO, CNPMA; FRANKLIN BEHLAU, Fundecitrus; LUIZ ALBERTO BERALDO MORAES, FFCLRP-USP. |
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Título: |
Bioguided isolation, characterization and media optimization for production of Lysolipins by actinomycete as antimicrobial compound against Xanthomonas citri subsp. citri. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Molecular Biology Reports, v. 45, n. 6, p. 2455?2467, 2018. |
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ISSN: |
1573-4978 |
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DOI: |
https://doi.org/10.1007/s11033-018-4411-5 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Citrus Canker disease is one of the most important disease in citrus production worldwide caused by gram-negative bacterial pathogen Xanthomonas citri subsp. citri, leading to great economic losses. Currently, a spray of copper-based bactericides is the primary measure for citrus canker management. However, these measures can lead to the contamination of soil by metal contamination, but also the development of copper-resistant Xanthomonas populations. Considering the need to discovery new alternatives to control the citrus canker disease, actinomycetes isolated from the Brazilian Caatinga biome and their crude extracts were tested against different strains of Xanthomonas citri subsp. citri. Streptomyces sp. Caat 1-54 crude extract showed the highest antibiotic activity against Xcc. The crude extract dereplication was performed by LC?MS/MS. Through bioassay-guided fractionation strategy, the antimicrobial activity was assigned to Lysolipins, showing a MIC around 0.4?0.8 µg/mL. Growth media optimization using statistical experimental design increased the Lysolipins production in three-fold production. The preventive and curative effects of the optimized crude extract obtained by experimental design of Caat-1-54 against citrus canker were evaluated in potted ?Pera? sweet orange nursery trees. Caat 1-54 extract was effective in preventing new infections by Xcc on leaves but was not able to reduce Xcc population in pre-established citrus canker lesions. Streptomyces sp. Caat 1-54 extract is a promising, environmentally-friendly source of antimicrobial compound to protect citrus trees against citrus canker. MenosAbstract: Citrus Canker disease is one of the most important disease in citrus production worldwide caused by gram-negative bacterial pathogen Xanthomonas citri subsp. citri, leading to great economic losses. Currently, a spray of copper-based bactericides is the primary measure for citrus canker management. However, these measures can lead to the contamination of soil by metal contamination, but also the development of copper-resistant Xanthomonas populations. Considering the need to discovery new alternatives to control the citrus canker disease, actinomycetes isolated from the Brazilian Caatinga biome and their crude extracts were tested against different strains of Xanthomonas citri subsp. citri. Streptomyces sp. Caat 1-54 crude extract showed the highest antibiotic activity against Xcc. The crude extract dereplication was performed by LC?MS/MS. Through bioassay-guided fractionation strategy, the antimicrobial activity was assigned to Lysolipins, showing a MIC around 0.4?0.8 µg/mL. Growth media optimization using statistical experimental design increased the Lysolipins production in three-fold production. The preventive and curative effects of the optimized crude extract obtained by experimental design of Caat-1-54 against citrus canker were evaluated in potted ?Pera? sweet orange nursery trees. Caat 1-54 extract was effective in preventing new infections by Xcc on leaves but was not able to reduce Xcc population in pre-established citrus canker lesions. Streptomyces sp.... Mostrar Tudo |
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Palavras-Chave: |
Antimicrobial agent; Citrus canker control. |
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Thesagro: |
Actinomiceto; Cancro cítrico; Controle biológico; Controle microbiano; Doença de planta. |
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Thesaurus Nal: |
Actinobacteria; Experimental design; Secondary metabolites; Xanthomonas citri subsp. citri. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02852naa a2200373 a 4500
001 2101438
005 2020-11-03
008 2018 bl uuuu u00u1 u #d
022 $a1573-4978
024 7 $ahttps://doi.org/10.1007/s11033-018-4411-5$2DOI
100 1 $aRODRIGUES, J. P.
245 $aBioguided isolation, characterization and media optimization for production of Lysolipins by actinomycete as antimicrobial compound against Xanthomonas citri subsp. citri.$h[electronic resource]
260 $c2018
520 $aAbstract: Citrus Canker disease is one of the most important disease in citrus production worldwide caused by gram-negative bacterial pathogen Xanthomonas citri subsp. citri, leading to great economic losses. Currently, a spray of copper-based bactericides is the primary measure for citrus canker management. However, these measures can lead to the contamination of soil by metal contamination, but also the development of copper-resistant Xanthomonas populations. Considering the need to discovery new alternatives to control the citrus canker disease, actinomycetes isolated from the Brazilian Caatinga biome and their crude extracts were tested against different strains of Xanthomonas citri subsp. citri. Streptomyces sp. Caat 1-54 crude extract showed the highest antibiotic activity against Xcc. The crude extract dereplication was performed by LC?MS/MS. Through bioassay-guided fractionation strategy, the antimicrobial activity was assigned to Lysolipins, showing a MIC around 0.4?0.8 µg/mL. Growth media optimization using statistical experimental design increased the Lysolipins production in three-fold production. The preventive and curative effects of the optimized crude extract obtained by experimental design of Caat-1-54 against citrus canker were evaluated in potted ?Pera? sweet orange nursery trees. Caat 1-54 extract was effective in preventing new infections by Xcc on leaves but was not able to reduce Xcc population in pre-established citrus canker lesions. Streptomyces sp. Caat 1-54 extract is a promising, environmentally-friendly source of antimicrobial compound to protect citrus trees against citrus canker.
650 $aActinobacteria
650 $aExperimental design
650 $aSecondary metabolites
650 $aXanthomonas citri subsp. citri
650 $aActinomiceto
650 $aCancro cítrico
650 $aControle biológico
650 $aControle microbiano
650 $aDoença de planta
653 $aAntimicrobial agent
653 $aCitrus canker control
700 1 $aPETI, A. P. F.
700 1 $aFIGUEIRÓ, F. S.
700 1 $aROCHA, I. S.
700 1 $aACQUARO JUNIOR, V. R.
700 1 $aSILVA, T. G.
700 1 $aMELO, I. S. de
700 1 $aBEHLAU, F.
700 1 $aMORAES, L. A. B.
773 $tMolecular Biology Reports$gv. 45, n. 6, p. 2455?2467, 2018.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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