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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
05/12/2018 |
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Data da última atualização: |
09/01/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FIGUEIREDO, A.; AGNOLON, I. C.; LOPES, L. G.; GIGLIOTI, R.; CHAGAS, A. C. de S. |
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Afiliação: |
Amanda Figueiredo, UNESP; Isabela Cabeça Agnolon, UNICEP; Louyse Gabrielli Lopes, UNICEP; Rodrigo Giglioti, UNESP; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
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Título: |
Comparative study of hatching estimation methods of Rhipicephalus (Boophilus) microplus eggs. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Veterinary Parasitology, v.264, p.35-38, dec. 2018. |
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ISSN: |
1873-2550 |
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Idioma: |
Inglês |
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Conteúdo: |
Reproductive parameters of Rhipicephalus (Boophilus) microplus are often evaluated. They are good indicators of resistance to commercial acaricides and of plant extracts? efficacy. The objective of this study was to compare the techniques: visual estimation and quantification by sampling used in the Adult Immersion Test (AIT) to calculate the hatching rate of eggs. Engorged females collected from cattle were subjected to the AIT with plant extracts and kept in an incubator for oviposition. The egg hatching was evaluated in 210 syringes by visual estimation (%). Then, eggs and larvae were counted into samples of 100 individuals, in three repetitions by stereo microscope. Significant differences were found between the two tests (p?0.05). The egg hatching average of visual estimation was higher than the quantification by sampling, 56.8?48.0, respectively (correlation=0.85). We found that the visual assessment leads to a higher estimate of larvae in relation to eggs, because the infertile eggs can be concealed in the center of the syringe. In quantification by sampling, no statistical differences (p=0.99) were observed in the pairwise counts between the three samples (48.1 ± 26.6%, 47.8 ± 26.9%, 48.1 ± 26.5%) (correlation of repetitions=0.96). This suggests that counting one sample is sufficient and the result should not differ much, regardless of the evaluator. Regarding the cutoff point of tick resistance status (95%), both methods are reliable. This study contributes to improvement of the AIT and can stimulate researchers to choose more accurate techniques for the assessment of egg hatching. MenosReproductive parameters of Rhipicephalus (Boophilus) microplus are often evaluated. They are good indicators of resistance to commercial acaricides and of plant extracts? efficacy. The objective of this study was to compare the techniques: visual estimation and quantification by sampling used in the Adult Immersion Test (AIT) to calculate the hatching rate of eggs. Engorged females collected from cattle were subjected to the AIT with plant extracts and kept in an incubator for oviposition. The egg hatching was evaluated in 210 syringes by visual estimation (%). Then, eggs and larvae were counted into samples of 100 individuals, in three repetitions by stereo microscope. Significant differences were found between the two tests (p?0.05). The egg hatching average of visual estimation was higher than the quantification by sampling, 56.8?48.0, respectively (correlation=0.85). We found that the visual assessment leads to a higher estimate of larvae in relation to eggs, because the infertile eggs can be concealed in the center of the syringe. In quantification by sampling, no statistical differences (p=0.99) were observed in the pairwise counts between the three samples (48.1 ± 26.6%, 47.8 ± 26.9%, 48.1 ± 26.5%) (correlation of repetitions=0.96). This suggests that counting one sample is sufficient and the result should not differ much, regardless of the evaluator. Regarding the cutoff point of tick resistance status (95%), both methods are reliable. This study contributes to impro... Mostrar Tudo |
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Palavras-Chave: |
Adult immersion test (AIT); Cattle tick; Methods of larvae estimation; Quantification by sampling; Rhipicephalus (Boophilus) microplus; Visual estimation. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/187834/1/ComparativeStudy.pdf
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Marc: |
LEADER 02408naa a2200253 a 4500
001 2100813
005 2019-01-09
008 2018 bl uuuu u00u1 u #d
022 $a1873-2550
100 1 $aFIGUEIREDO, A.
245 $aComparative study of hatching estimation methods of Rhipicephalus (Boophilus) microplus eggs.$h[electronic resource]
260 $c2018
520 $aReproductive parameters of Rhipicephalus (Boophilus) microplus are often evaluated. They are good indicators of resistance to commercial acaricides and of plant extracts? efficacy. The objective of this study was to compare the techniques: visual estimation and quantification by sampling used in the Adult Immersion Test (AIT) to calculate the hatching rate of eggs. Engorged females collected from cattle were subjected to the AIT with plant extracts and kept in an incubator for oviposition. The egg hatching was evaluated in 210 syringes by visual estimation (%). Then, eggs and larvae were counted into samples of 100 individuals, in three repetitions by stereo microscope. Significant differences were found between the two tests (p?0.05). The egg hatching average of visual estimation was higher than the quantification by sampling, 56.8?48.0, respectively (correlation=0.85). We found that the visual assessment leads to a higher estimate of larvae in relation to eggs, because the infertile eggs can be concealed in the center of the syringe. In quantification by sampling, no statistical differences (p=0.99) were observed in the pairwise counts between the three samples (48.1 ± 26.6%, 47.8 ± 26.9%, 48.1 ± 26.5%) (correlation of repetitions=0.96). This suggests that counting one sample is sufficient and the result should not differ much, regardless of the evaluator. Regarding the cutoff point of tick resistance status (95%), both methods are reliable. This study contributes to improvement of the AIT and can stimulate researchers to choose more accurate techniques for the assessment of egg hatching.
653 $aAdult immersion test (AIT)
653 $aCattle tick
653 $aMethods of larvae estimation
653 $aQuantification by sampling
653 $aRhipicephalus (Boophilus) microplus
653 $aVisual estimation
700 1 $aAGNOLON, I. C.
700 1 $aLOPES, L. G.
700 1 $aGIGLIOTI, R.
700 1 $aCHAGAS, A. C. de S.
773 $tVeterinary Parasitology$gv.264, p.35-38, dec. 2018.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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| Registros recuperados : 440 | |
| 45. |  | POSTIGO, M. P.; CHAGAS, A. C. de S.; OLIVEIRA, M. C. de S.; COLNAGO, L. A. Estudos metabolômicos em bovinos empregando RMN de alta resolução e quimiometria. In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 2., 2010, São Carlos, SP. Anais... São Carlos: Embrapa Instrumentação Agropecuária: Embrapa Pecuária Sudeste, 2010. p. 78. (Embrapa Instrumentação Agropecuária. Documentos, 50).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação; Embrapa Pecuária Sudeste. |
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| 48. | | CAVALCANTE, A. A.; LOPES, L. G.; CHAGAS, A. C. de S.; OIANO NETO, J. Extratos vegetais concentrados de média polaridade com ação larvicida frente ao carrapato bovino Rhipicephalus (Boophilus) microplus. In: JORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 9., 2017, São Carlos, SP. Anais... São Carlos, SP: Embrapa Pecuária Sudeste; Embrapa Instrumentação, 2017. p. 11. (Embrapa Pecuária Sudeste. Documentos, 126). Editores técnicos: Alexandre Berndt, Ana Rita Araujo Nogueira, Bianca Baccili Zanotto Vigna, Juliana Gonçalves Costa, Lea Chapaval, Manuel Antonio Chagas Jacinto, Patricia Menezes Santos.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 52. | | SANTOS, L. A. L. dos; GAINZA, Y. A.; CHAGAS, A. C. de S.; MINHO, A. P. Efeito deletério de extratos de acácia sobre o desenvolvimento larvar de Haemonchus contortus. In: JORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 11., 2019, São Carlos, SP. Anais... São Carlos: Embrapa Pecuária Sudeste: Embrapa Instrumentação, 2019. p. 40 (Embrapa Pecuária Sudeste. Documentos, 134).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 53. | | ALEMÁN GAINZA, Y.; CHAPAVAL, L.; ESTELLA, B. M.; VACCARI, N. A.; CHAGAS, A. C. de S. Detection of bacterial contaminants by the larval development test. In: ENCONTRO CIENTÍFICO DE PRODUÇÃO ANIMAL SUSTENTÁVEL, 7., 2017, Campinas, SP . Anais... Nova Odessa, SP: Instituto de Zootecnia, 2017. Boletim de Indústria Animal, v. 74.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 54. | | ALEMÁN GAINZA, Y.; FANTATTO, R. R.; NICIURA, S. C. M.; CHAGAS, A. C. de S. Determination of parasite resistance status to anthelmintics through the larval development test. In: ENCONTRO CIENTÍFICO DE PRODUÇÃO ANIMAL SUSTENTÁVEL, 7., 2017, Campinas, SP . Anais... Nova Odessa, SP: Instituto de Zootecnia, 2017. Boletim de Indústria Animal, v. 74.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 57. | | CHAGAS, A. C. de S.; LEITE, R. C.; FURLONG, J.; PRATES, H. T.; PASSOS, W. M. Sensibilidade do carrapato Boophilus microplus a solventes. Ciência Rural, Santa Maria, v. 33, n. 1, p. 109-114, 2003.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| Registros recuperados : 440 | |
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