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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
04/12/2018 |
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Data da última atualização: |
16/01/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PEREIRA, V. A.; FORTE, J. M.; ARRUDA-JÚNIOR, J. P. V.; DINIZ, F. M.; MAGGIONI, R.; SALMITO-VANDERLEY, C. S. B. |
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Afiliação: |
Vanessa Alves Pereira, Pós-graduação - Universidade Estadual do Ceará (UECE) - Fortaleza, CE, Brazil; Jamille Martins Forte, Instituto de Ciências do Mar (LABOMAR), Universidade Federal do Ceará (UFC) - Fortaleza, CE, Brazil; José Pedro Vieira Arruda-Júnior, Universidade Federal do Ceará (UFC) - Fortaleza, CE, Brazil; FABIO MENDONCA DINIZ, CNPC; Rodrigo Maggioni, Instituto de Ciências do Mar (LABOMAR), Universidade Federal do Ceará (UFC) - Fortaleza, CE, Brazil; C. S. B. Salmito-Vanderley, Pós-graduação - Universidade Estadual do Ceará (UECE) - Fortaleza, CE, Brazil. |
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Título: |
Identification and characterization of microsatellite loci in West Atlantic sea cucumber Holothuria grisea (Selenka 1867). |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Journal of Genetics, v. 97, n. 5, p. 1363-1369, Dec. 2018. |
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DOI: |
10.1007/s12041-018-1022-9 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The sea cucumber Holothuria grisea has become the subject of intense and unregulated fishing in northeastern Brazil due to their growing demand in Asian market. However, there is little knowledge about the dynamics and genetics of H. grisea wild populations on the South American coast. In this study, we present the first set of H. grisea microsatellite markers, identified and characterized using Illumina paired-end reads of whole genome shotgun sequencing. From 50 strictly selected candidates, eight novel microsatellite markers were successfully developed. We then genotyped 30 individuals to evaluate the degree of polymorphism and validate the markers. The number of alleles ranged from three to 14, while observed and expected heterozygotes ranged from 0.156 to 0.906 and from 0.283 to 0.774, respectively. After correcting for multiple tests, we found no evidence of linkage disequilibrium in all pairwise combinations between the loci. One locus (Hgr15607) revealed deviation from the Hardy?Weinberg equilibrium, as well as the presence of null alleles. However, we observed significant differences in frequency distribution between males and females at locus Hgr15607. We believe that the markers described here will be useful for conservation efforts and management of H. grisea fisheries and for prospective aquaculture of these organisms. |
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Palavras-Chave: |
Genetic diversity; Holothuria grisea; Microsatellite; Next-generation sequencing; Sea cucumber. |
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Thesaurus Nal: |
Animal genetics. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02213naa a2200265 a 4500
001 2100654
005 2019-01-16
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1007/s12041-018-1022-9$2DOI
100 1 $aPEREIRA, V. A.
245 $aIdentification and characterization of microsatellite loci in West Atlantic sea cucumber Holothuria grisea (Selenka 1867).$h[electronic resource]
260 $c2018
520 $aAbstract: The sea cucumber Holothuria grisea has become the subject of intense and unregulated fishing in northeastern Brazil due to their growing demand in Asian market. However, there is little knowledge about the dynamics and genetics of H. grisea wild populations on the South American coast. In this study, we present the first set of H. grisea microsatellite markers, identified and characterized using Illumina paired-end reads of whole genome shotgun sequencing. From 50 strictly selected candidates, eight novel microsatellite markers were successfully developed. We then genotyped 30 individuals to evaluate the degree of polymorphism and validate the markers. The number of alleles ranged from three to 14, while observed and expected heterozygotes ranged from 0.156 to 0.906 and from 0.283 to 0.774, respectively. After correcting for multiple tests, we found no evidence of linkage disequilibrium in all pairwise combinations between the loci. One locus (Hgr15607) revealed deviation from the Hardy?Weinberg equilibrium, as well as the presence of null alleles. However, we observed significant differences in frequency distribution between males and females at locus Hgr15607. We believe that the markers described here will be useful for conservation efforts and management of H. grisea fisheries and for prospective aquaculture of these organisms.
650 $aAnimal genetics
653 $aGenetic diversity
653 $aHolothuria grisea
653 $aMicrosatellite
653 $aNext-generation sequencing
653 $aSea cucumber
700 1 $aFORTE, J. M.
700 1 $aARRUDA-JÚNIOR, J. P. V.
700 1 $aDINIZ, F. M.
700 1 $aMAGGIONI, R.
700 1 $aSALMITO-VANDERLEY, C. S. B.
773 $tJournal of Genetics$gv. 97, n. 5, p. 1363-1369, Dec. 2018.
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