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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
30/08/2018 |
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Data da última atualização: |
30/08/2018 |
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Autoria: |
SANTOS, A. C.; LEITE, F. P. L.; VIANNA, A. M.; WEEGE, G. B.; FINGER, I. S.; MULLER, V.; CURCIO, B. R.; NOGUEIRA, C. E. W. |
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Afiliação: |
Alice C. Santos, Faculdade de Veterinária/Universidade Federal de Pelotas - UFPel; Fábio P. L. Leite, Centro de Desenvolvimento Tecnológico - UFPel; Ana M. Vianna, Centro de Desenvolvimento Tecnológico - UFPel; Guilherme B. Weege, Laboratório de Parasitologia Molecular e Imunologia - UFPel; Ilusca S. Finger, Centro de Desenvolvimento Tecnológico - UFPel; Vitória Müller, Faculdade de Veterinária/Universidade Federal de Pelotas - UFPel; Bruna R. Curcio, Faculdade de Veterinária/Universidade Federal de Pelotas - UFPel; Carlos E. W. Nogueira, Faculdade de Veterinária/Universidade Federal de Pelotas - UFPel. |
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Título: |
Dynamics of humoral immune response in pregnant mares and foals vaccinated with Theileria equi recombinant EMA-2. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 6, p. 1105-1109, junho 2018 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Dinâmica da resposta imune humoral em éguas gestantes e potros vacinados com EMA-2 recombinante de Theileria equi. |
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Conteúdo: |
Theileria equi is an infectious hemoprotozoan agent of equine piroplasmosis, a disease that has severe economic and sanitary impact internationally. In addition to its common clinical features, piroplasmosis can cause gestational losses and neonatal damage, which makes neonates susceptible to this disease. The aim of this study was to evaluate the dynamics of humoral immune response to recombinant EMA-2 of T. equi in pregnant mares and foals, as well as the transfer of vaccine antibodies through the colostrum ingested by sucking foals. For vaccine production, the EMA-2 expression gene was cloned and expressed in the yeast species, Pichia pastoris. Thirty-six horses were used, of which 18 were pregnant mares and 18 were foals. The mares were divided into control and vaccinated groups, and the vaccinated group received three doses of rEMA-2 every 21 days starting at 300 days of gestation. Foals from vaccinated and control groups were evaluated until the sixth month of life. The production of antibodies by foals on the rEMA-2 vaccination schedule was also evaluated from the second month of life. Foals in the vaccinated group had received three doses of the vaccine every 21 days. The method used to evaluate serum and colostrum samples was indirect ELISA, and plates were sensitized with the rEMA-2 protein. At the end of the vaccination schedule, vaccinated mares showed a 2.3-fold increase in antibody levels when compared to baseline values. The colostrum of vaccinated mares presented antibody levels of 1.0432±0.33. Foals delivered by vaccinated mares presented levels of antibodies greater than those of foals delivered by control mares after their first time sucking (at about twelve hours after birth). Foals vaccinated in the second month of life showed an 8.3-fold increase in antibody levels when compared to baseline values. The vaccination schedule with rEMA-2 was able to stimulate humoral immunity in pregnant mares. Vaccine immunoglobins were concentrated in the colostrum of vaccinated mares and foals delivered by these mares showed an increase in serum levels of vaccine antibodies after the first-time sucking. MenosTheileria equi is an infectious hemoprotozoan agent of equine piroplasmosis, a disease that has severe economic and sanitary impact internationally. In addition to its common clinical features, piroplasmosis can cause gestational losses and neonatal damage, which makes neonates susceptible to this disease. The aim of this study was to evaluate the dynamics of humoral immune response to recombinant EMA-2 of T. equi in pregnant mares and foals, as well as the transfer of vaccine antibodies through the colostrum ingested by sucking foals. For vaccine production, the EMA-2 expression gene was cloned and expressed in the yeast species, Pichia pastoris. Thirty-six horses were used, of which 18 were pregnant mares and 18 were foals. The mares were divided into control and vaccinated groups, and the vaccinated group received three doses of rEMA-2 every 21 days starting at 300 days of gestation. Foals from vaccinated and control groups were evaluated until the sixth month of life. The production of antibodies by foals on the rEMA-2 vaccination schedule was also evaluated from the second month of life. Foals in the vaccinated group had received three doses of the vaccine every 21 days. The method used to evaluate serum and colostrum samples was indirect ELISA, and plates were sensitized with the rEMA-2 protein. At the end of the vaccination schedule, vaccinated mares showed a 2.3-fold increase in antibody levels when compared to baseline values. The colostrum of vaccinated mares prese... Mostrar Tudo |
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Palavras-Chave: |
Hemoprotozoan; Hemoprotozoário; Imunidade humoral; Piroplasmose equina; REMA-2. |
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Thesagro: |
Eqüino. |
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Thesaurus Nal: |
Equine piroplasmosis; Horses; Humoral immunity; Parasitoses; Theileria equi. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/182208/1/Dynamics-of-humoral-immune-response-in-pregnant-mares.pdf
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Marc: |
LEADER 03294naa a2200349 a 4500
001 2094866
005 2018-08-30
008 2018 bl uuuu u00u1 u #d
100 1 $aSANTOS, A. C.
245 $aDynamics of humoral immune response in pregnant mares and foals vaccinated with Theileria equi recombinant EMA-2.$h[electronic resource]
260 $c2018
500 $aTítulo em português: Dinâmica da resposta imune humoral em éguas gestantes e potros vacinados com EMA-2 recombinante de Theileria equi.
520 $aTheileria equi is an infectious hemoprotozoan agent of equine piroplasmosis, a disease that has severe economic and sanitary impact internationally. In addition to its common clinical features, piroplasmosis can cause gestational losses and neonatal damage, which makes neonates susceptible to this disease. The aim of this study was to evaluate the dynamics of humoral immune response to recombinant EMA-2 of T. equi in pregnant mares and foals, as well as the transfer of vaccine antibodies through the colostrum ingested by sucking foals. For vaccine production, the EMA-2 expression gene was cloned and expressed in the yeast species, Pichia pastoris. Thirty-six horses were used, of which 18 were pregnant mares and 18 were foals. The mares were divided into control and vaccinated groups, and the vaccinated group received three doses of rEMA-2 every 21 days starting at 300 days of gestation. Foals from vaccinated and control groups were evaluated until the sixth month of life. The production of antibodies by foals on the rEMA-2 vaccination schedule was also evaluated from the second month of life. Foals in the vaccinated group had received three doses of the vaccine every 21 days. The method used to evaluate serum and colostrum samples was indirect ELISA, and plates were sensitized with the rEMA-2 protein. At the end of the vaccination schedule, vaccinated mares showed a 2.3-fold increase in antibody levels when compared to baseline values. The colostrum of vaccinated mares presented antibody levels of 1.0432±0.33. Foals delivered by vaccinated mares presented levels of antibodies greater than those of foals delivered by control mares after their first time sucking (at about twelve hours after birth). Foals vaccinated in the second month of life showed an 8.3-fold increase in antibody levels when compared to baseline values. The vaccination schedule with rEMA-2 was able to stimulate humoral immunity in pregnant mares. Vaccine immunoglobins were concentrated in the colostrum of vaccinated mares and foals delivered by these mares showed an increase in serum levels of vaccine antibodies after the first-time sucking.
650 $aEquine piroplasmosis
650 $aHorses
650 $aHumoral immunity
650 $aParasitoses
650 $aTheileria equi
650 $aEqüino
653 $aHemoprotozoan
653 $aHemoprotozoário
653 $aImunidade humoral
653 $aPiroplasmose equina
653 $aREMA-2
700 1 $aLEITE, F. P. L.
700 1 $aVIANNA, A. M.
700 1 $aWEEGE, G. B.
700 1 $aFINGER, I. S.
700 1 $aMULLER, V.
700 1 $aCURCIO, B. R.
700 1 $aNOGUEIRA, C. E. W.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 38, n. 6, p. 1105-1109, junho 2018
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo; Embrapa Pesca e Aquicultura. |
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Data corrente: |
19/12/2019 |
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Data da última atualização: |
07/04/2020 |
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Tipo da produção científica: |
Circular Técnica |
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Autoria: |
COSTA, R. V. da; CAMPOS, L. J. M.; ALMEIDA, R. E. M. de; PEREIRA, L. A. O. A.; COTA, L. V.; SILVA, D. D. da; BERNARDES, F. P.; AMORIM, F. R. de. |
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Afiliação: |
RODRIGO VERAS DA COSTA, CNPMS; LEONARDO JOSE MOTTA CAMPOS, CNPSO; RODRIGO ESTEVAM MUNHOZ DE ALMEIDA, CNPASA; Lucas André Oliveira Almeida Pereira, Estagiário da Embrapa Soja; LUCIANO VIANA COTA, CNPMS; DAGMA DIONISIA DA SILVA, CNPMS; Fernanda Pinheiro Bernardes, Estagiária da Embrapa Milho e Sorgo; Felipe Rosa de Amorim, Estagiário da Embrapa Milho e Sorgo. |
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Título: |
Comportamento de híbridos de milho na safrinha em Tocantins. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Sete Lagoas: Embrapa Milho e Sorgo, 2019. |
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Série: |
(Embrapa Milho e Sorgo. Circular Técnica, 258). |
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Idioma: |
Português |
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Conteúdo: |
As características dos híbridos, como o ciclo, o potencial e a estabilidade produtiva e a reação das pragas e doenças, devem ser consideradas em cada localidade. Além disso, em razão da grande quantidade de híbridos disponíveis, assim como a variabilidade de suas características agronômicas, é necessário um estudo de avaliação para identificar as cultivares mais bem adaptadas a cada condição de semeadura. De acordo com os resultados, nota-se que a época de semeadura é um dos principais fatores que afetam a produtividade do milho safrinha no Tocantins. Quanto mais tardia for realizada a semeadura, principalmente a partir de meados de fevereiro, maiores serão os riscos de restrições hídricas e, consequentemente, de obtenção de baixas produtividades. O atraso na época de semeadura reduziu a produtividade dos híbridos. |
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Palavras-Chave: |
Tocantins. |
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Thesagro: |
Hibrido; Milho; Safrinha; Variedade; Zea Mays. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/212211/1/CircTec-258.pdf
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Marc: |
LEADER 01690nam a2200277 a 4500
001 2117380
005 2020-04-07
008 2019 bl uuuu u0uu1 u #d
100 1 $aCOSTA, R. V. da
245 $aComportamento de híbridos de milho na safrinha em Tocantins.$h[electronic resource]
260 $aSete Lagoas: Embrapa Milho e Sorgo$c2019
490 $a(Embrapa Milho e Sorgo. Circular Técnica, 258).
520 $aAs características dos híbridos, como o ciclo, o potencial e a estabilidade produtiva e a reação das pragas e doenças, devem ser consideradas em cada localidade. Além disso, em razão da grande quantidade de híbridos disponíveis, assim como a variabilidade de suas características agronômicas, é necessário um estudo de avaliação para identificar as cultivares mais bem adaptadas a cada condição de semeadura. De acordo com os resultados, nota-se que a época de semeadura é um dos principais fatores que afetam a produtividade do milho safrinha no Tocantins. Quanto mais tardia for realizada a semeadura, principalmente a partir de meados de fevereiro, maiores serão os riscos de restrições hídricas e, consequentemente, de obtenção de baixas produtividades. O atraso na época de semeadura reduziu a produtividade dos híbridos.
650 $aHibrido
650 $aMilho
650 $aSafrinha
650 $aVariedade
650 $aZea Mays
653 $aTocantins
700 1 $aCAMPOS, L. J. M.
700 1 $aALMEIDA, R. E. M. de
700 1 $aPEREIRA, L. A. O. A.
700 1 $aCOTA, L. V.
700 1 $aSILVA, D. D. da
700 1 $aBERNARDES, F. P.
700 1 $aAMORIM, F. R. de
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Embrapa Milho e Sorgo (CNPMS) |
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