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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
30/08/2018 |
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Data da última atualização: |
30/08/2018 |
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Autoria: |
SANTOS, A. C.; LEITE, F. P. L.; VIANNA, A. M.; WEEGE, G. B.; FINGER, I. S.; MULLER, V.; CURCIO, B. R.; NOGUEIRA, C. E. W. |
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Afiliação: |
Alice C. Santos, Faculdade de Veterinária/Universidade Federal de Pelotas - UFPel; Fábio P. L. Leite, Centro de Desenvolvimento Tecnológico - UFPel; Ana M. Vianna, Centro de Desenvolvimento Tecnológico - UFPel; Guilherme B. Weege, Laboratório de Parasitologia Molecular e Imunologia - UFPel; Ilusca S. Finger, Centro de Desenvolvimento Tecnológico - UFPel; Vitória Müller, Faculdade de Veterinária/Universidade Federal de Pelotas - UFPel; Bruna R. Curcio, Faculdade de Veterinária/Universidade Federal de Pelotas - UFPel; Carlos E. W. Nogueira, Faculdade de Veterinária/Universidade Federal de Pelotas - UFPel. |
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Título: |
Dynamics of humoral immune response in pregnant mares and foals vaccinated with Theileria equi recombinant EMA-2. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 6, p. 1105-1109, junho 2018 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Dinâmica da resposta imune humoral em éguas gestantes e potros vacinados com EMA-2 recombinante de Theileria equi. |
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Conteúdo: |
Theileria equi is an infectious hemoprotozoan agent of equine piroplasmosis, a disease that has severe economic and sanitary impact internationally. In addition to its common clinical features, piroplasmosis can cause gestational losses and neonatal damage, which makes neonates susceptible to this disease. The aim of this study was to evaluate the dynamics of humoral immune response to recombinant EMA-2 of T. equi in pregnant mares and foals, as well as the transfer of vaccine antibodies through the colostrum ingested by sucking foals. For vaccine production, the EMA-2 expression gene was cloned and expressed in the yeast species, Pichia pastoris. Thirty-six horses were used, of which 18 were pregnant mares and 18 were foals. The mares were divided into control and vaccinated groups, and the vaccinated group received three doses of rEMA-2 every 21 days starting at 300 days of gestation. Foals from vaccinated and control groups were evaluated until the sixth month of life. The production of antibodies by foals on the rEMA-2 vaccination schedule was also evaluated from the second month of life. Foals in the vaccinated group had received three doses of the vaccine every 21 days. The method used to evaluate serum and colostrum samples was indirect ELISA, and plates were sensitized with the rEMA-2 protein. At the end of the vaccination schedule, vaccinated mares showed a 2.3-fold increase in antibody levels when compared to baseline values. The colostrum of vaccinated mares presented antibody levels of 1.0432±0.33. Foals delivered by vaccinated mares presented levels of antibodies greater than those of foals delivered by control mares after their first time sucking (at about twelve hours after birth). Foals vaccinated in the second month of life showed an 8.3-fold increase in antibody levels when compared to baseline values. The vaccination schedule with rEMA-2 was able to stimulate humoral immunity in pregnant mares. Vaccine immunoglobins were concentrated in the colostrum of vaccinated mares and foals delivered by these mares showed an increase in serum levels of vaccine antibodies after the first-time sucking. MenosTheileria equi is an infectious hemoprotozoan agent of equine piroplasmosis, a disease that has severe economic and sanitary impact internationally. In addition to its common clinical features, piroplasmosis can cause gestational losses and neonatal damage, which makes neonates susceptible to this disease. The aim of this study was to evaluate the dynamics of humoral immune response to recombinant EMA-2 of T. equi in pregnant mares and foals, as well as the transfer of vaccine antibodies through the colostrum ingested by sucking foals. For vaccine production, the EMA-2 expression gene was cloned and expressed in the yeast species, Pichia pastoris. Thirty-six horses were used, of which 18 were pregnant mares and 18 were foals. The mares were divided into control and vaccinated groups, and the vaccinated group received three doses of rEMA-2 every 21 days starting at 300 days of gestation. Foals from vaccinated and control groups were evaluated until the sixth month of life. The production of antibodies by foals on the rEMA-2 vaccination schedule was also evaluated from the second month of life. Foals in the vaccinated group had received three doses of the vaccine every 21 days. The method used to evaluate serum and colostrum samples was indirect ELISA, and plates were sensitized with the rEMA-2 protein. At the end of the vaccination schedule, vaccinated mares showed a 2.3-fold increase in antibody levels when compared to baseline values. The colostrum of vaccinated mares prese... Mostrar Tudo |
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Palavras-Chave: |
Hemoprotozoan; Hemoprotozoário; Imunidade humoral; Piroplasmose equina; REMA-2. |
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Thesagro: |
Eqüino. |
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Thesaurus Nal: |
Equine piroplasmosis; Horses; Humoral immunity; Parasitoses; Theileria equi. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/182208/1/Dynamics-of-humoral-immune-response-in-pregnant-mares.pdf
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Marc: |
LEADER 03294naa a2200349 a 4500
001 2094866
005 2018-08-30
008 2018 bl uuuu u00u1 u #d
100 1 $aSANTOS, A. C.
245 $aDynamics of humoral immune response in pregnant mares and foals vaccinated with Theileria equi recombinant EMA-2.$h[electronic resource]
260 $c2018
500 $aTítulo em português: Dinâmica da resposta imune humoral em éguas gestantes e potros vacinados com EMA-2 recombinante de Theileria equi.
520 $aTheileria equi is an infectious hemoprotozoan agent of equine piroplasmosis, a disease that has severe economic and sanitary impact internationally. In addition to its common clinical features, piroplasmosis can cause gestational losses and neonatal damage, which makes neonates susceptible to this disease. The aim of this study was to evaluate the dynamics of humoral immune response to recombinant EMA-2 of T. equi in pregnant mares and foals, as well as the transfer of vaccine antibodies through the colostrum ingested by sucking foals. For vaccine production, the EMA-2 expression gene was cloned and expressed in the yeast species, Pichia pastoris. Thirty-six horses were used, of which 18 were pregnant mares and 18 were foals. The mares were divided into control and vaccinated groups, and the vaccinated group received three doses of rEMA-2 every 21 days starting at 300 days of gestation. Foals from vaccinated and control groups were evaluated until the sixth month of life. The production of antibodies by foals on the rEMA-2 vaccination schedule was also evaluated from the second month of life. Foals in the vaccinated group had received three doses of the vaccine every 21 days. The method used to evaluate serum and colostrum samples was indirect ELISA, and plates were sensitized with the rEMA-2 protein. At the end of the vaccination schedule, vaccinated mares showed a 2.3-fold increase in antibody levels when compared to baseline values. The colostrum of vaccinated mares presented antibody levels of 1.0432±0.33. Foals delivered by vaccinated mares presented levels of antibodies greater than those of foals delivered by control mares after their first time sucking (at about twelve hours after birth). Foals vaccinated in the second month of life showed an 8.3-fold increase in antibody levels when compared to baseline values. The vaccination schedule with rEMA-2 was able to stimulate humoral immunity in pregnant mares. Vaccine immunoglobins were concentrated in the colostrum of vaccinated mares and foals delivered by these mares showed an increase in serum levels of vaccine antibodies after the first-time sucking.
650 $aEquine piroplasmosis
650 $aHorses
650 $aHumoral immunity
650 $aParasitoses
650 $aTheileria equi
650 $aEqüino
653 $aHemoprotozoan
653 $aHemoprotozoário
653 $aImunidade humoral
653 $aPiroplasmose equina
653 $aREMA-2
700 1 $aLEITE, F. P. L.
700 1 $aVIANNA, A. M.
700 1 $aWEEGE, G. B.
700 1 $aFINGER, I. S.
700 1 $aMULLER, V.
700 1 $aCURCIO, B. R.
700 1 $aNOGUEIRA, C. E. W.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 38, n. 6, p. 1105-1109, junho 2018
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Embrapa Unidades Centrais (AI-SEDE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
20/09/1996 |
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Data da última atualização: |
08/08/2018 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
LIMA, J. A. S.; OLIVEIRA, S. M. A.; TAVARES, S. C. C. de H. |
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Afiliação: |
SELMA CAVALCANTI CRUZ DE H TAVARES, CPATSA. |
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Título: |
Identificacao de fontes de resistencia em frutos de mangueiras (Mangifera indica) ao Botryodiplodia theobromae. |
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Ano de publicação: |
1996 |
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Fonte/Imprenta: |
Fitopatologia Brasileira, Brasília, DF, v. 21, p. 367, ago. 1996. |
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Idioma: |
Português |
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Notas: |
Suplemento. Edição dos Resumos do 29 Congresso Brasileiro de Fitopatoligia, Campo Grande, MS, ago. 1996. |
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Conteúdo: |
Botryodiplodia theobromae tem ocorrido nas areas irrigadas no Nordeste de forma significativa em cultura de importancia economica, taix como: videira, abacateiro, goiabeira, citros, coqueiro, bananeira e mangueira, mostrando-se bastante agressivo e infectando varias partes da planta. Visando identificar possiveis fontes de resistencia em frutos de seis variedades de mangueira (haden, Keitt, van dick, manguito, espada e rosa), procedeu-se a inoculacao dos frutos, previamente desinfectados com hipoclorito de sodio a 2% e feridos com um estilete flambado, com tres isolados de B. theobromae, procedentes da UFRPE (UFRPE/95), Petrolina, PE (44/94) e Teresina, PI (163/92). Os tres isolados mostraram patogenicos aos frutos das variedades testadas exibindo sintomas 24 horas apos a inoculacao. As variedades rosa, espada, van dick e manguito foram resistentes ao isolado 163/92, enquanto que a haden e keitt foram medianamente resistentes ao mesmo. Com relacao ao isolado 44/94, observou-se que as variedades espada e manguito foram resistentes a este isolado, rosa e hadem medianamente resistentes, keitt suscetivel e van dick altamente suscetivel. Para o isolado UFRPE/95, as variedades espada, manguido e haden, mostraram-se resistentes, rosa e keitt, medianemente resistentes, enquanto que a variedade van dick mostrou-se suscetivel a este isolado de B. theobromae. |
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Palavras-Chave: |
Botrydiplodia theobromae; Disease; Mango; Resistance. |
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Thesagro: |
Doença; Manga; Mangifera Indica; Resistência. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/132347/1/FitopatologiaBrasileirav.21p.3671996.pdf
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Marc: |
LEADER 02177nam a2200241 a 4500
001 1132347
005 2018-08-08
008 1996 bl uuuu u00u1 u #d
100 1 $aLIMA, J. A. S.
245 $aIdentificacao de fontes de resistencia em frutos de mangueiras (Mangifera indica) ao Botryodiplodia theobromae.
260 $aFitopatologia Brasileira, Brasília, DF, v. 21, p. 367, ago. 1996.$c1996
500 $aSuplemento. Edição dos Resumos do 29 Congresso Brasileiro de Fitopatoligia, Campo Grande, MS, ago. 1996.
520 $aBotryodiplodia theobromae tem ocorrido nas areas irrigadas no Nordeste de forma significativa em cultura de importancia economica, taix como: videira, abacateiro, goiabeira, citros, coqueiro, bananeira e mangueira, mostrando-se bastante agressivo e infectando varias partes da planta. Visando identificar possiveis fontes de resistencia em frutos de seis variedades de mangueira (haden, Keitt, van dick, manguito, espada e rosa), procedeu-se a inoculacao dos frutos, previamente desinfectados com hipoclorito de sodio a 2% e feridos com um estilete flambado, com tres isolados de B. theobromae, procedentes da UFRPE (UFRPE/95), Petrolina, PE (44/94) e Teresina, PI (163/92). Os tres isolados mostraram patogenicos aos frutos das variedades testadas exibindo sintomas 24 horas apos a inoculacao. As variedades rosa, espada, van dick e manguito foram resistentes ao isolado 163/92, enquanto que a haden e keitt foram medianamente resistentes ao mesmo. Com relacao ao isolado 44/94, observou-se que as variedades espada e manguito foram resistentes a este isolado, rosa e hadem medianamente resistentes, keitt suscetivel e van dick altamente suscetivel. Para o isolado UFRPE/95, as variedades espada, manguido e haden, mostraram-se resistentes, rosa e keitt, medianemente resistentes, enquanto que a variedade van dick mostrou-se suscetivel a este isolado de B. theobromae.
650 $aDoença
650 $aManga
650 $aMangifera Indica
650 $aResistência
653 $aBotrydiplodia theobromae
653 $aDisease
653 $aMango
653 $aResistance
700 1 $aOLIVEIRA, S. M. A.
700 1 $aTAVARES, S. C. C. de H.
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