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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
20/08/2018 |
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Data da última atualização: |
20/08/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CHITARRA, C. S.; OLIVEIRA FILHO, J. X. de; MORES, N.; SILVA, M. I. V. da; CÂNDIDO, S. L.; CEZARINO, P. G.; NAKAZATO, L.; DUTRA, V. |
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Afiliação: |
CRISTIANE SILVA CHITARRA; JOÃO XAVIER DE OLIVEIRA FILHO; NELSON MORES, CNPSA; MAYARA INÁCIO VINCENZI DA SILVA; STHEFANO LUIS CÂNDIDO; PAULA GABRIELA CEZARINO; LUCIANO NAKAZATO; VALERIA DUTRA. |
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Título: |
Identification of Pasteurella multocida transcribed genes in porcine lungs through RNAseq. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Microbial Pathogenisis, v. 122, p. 180-183, 2018. |
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DOI: |
10.1016/j.micpath.2018.06.014. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Pasteurella multocida is one of the most important pathogen that causes pneumonia in swine. Although several virulence factors are known, the pathogenesis of this bacterium is not well-studied. Therefore, to study the pathogenesis of P. multocida infection in porcine lung, next-generation RNA sequencing was used to compare the transcriptomes of P. multocida grown in vivo and in vitro, respectively. After P. multocida infection a total of 704 genes were expressed in vitro, 1422 genes were expressed in vivo, and 237 genes were differentially expressed based on statistical analyses, padj of ≤0.1. Genes encoding ribosomal proteins or other products that function in the regulation of transcription and translation were downregulated, whereas genes whose products affected cellular processes (protein transport and RNA degradation) and metabolic pathways, such as those of amino acid metabolism and nucleotide metabolism, were upregulated in vitro compared with in vivo. This study shows that differentially expressed genes in P. multocida regulate pathways that operate during stress, iron capture, heat shock, and nitrogen regulation. However, extensive investigation of the pathogenic mechanism of P. multocida is still required. |
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Thesagro: |
Pneumonia; Sanidade Animal; Suíno. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01993naa a2200253 a 4500
001 2094234
005 2018-08-20
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1016/j.micpath.2018.06.014.$2DOI
100 1 $aCHITARRA, C. S.
245 $aIdentification of Pasteurella multocida transcribed genes in porcine lungs through RNAseq.$h[electronic resource]
260 $c2018
520 $aAbstract: Pasteurella multocida is one of the most important pathogen that causes pneumonia in swine. Although several virulence factors are known, the pathogenesis of this bacterium is not well-studied. Therefore, to study the pathogenesis of P. multocida infection in porcine lung, next-generation RNA sequencing was used to compare the transcriptomes of P. multocida grown in vivo and in vitro, respectively. After P. multocida infection a total of 704 genes were expressed in vitro, 1422 genes were expressed in vivo, and 237 genes were differentially expressed based on statistical analyses, padj of ≤0.1. Genes encoding ribosomal proteins or other products that function in the regulation of transcription and translation were downregulated, whereas genes whose products affected cellular processes (protein transport and RNA degradation) and metabolic pathways, such as those of amino acid metabolism and nucleotide metabolism, were upregulated in vitro compared with in vivo. This study shows that differentially expressed genes in P. multocida regulate pathways that operate during stress, iron capture, heat shock, and nitrogen regulation. However, extensive investigation of the pathogenic mechanism of P. multocida is still required.
650 $aPneumonia
650 $aSanidade Animal
650 $aSuíno
700 1 $aOLIVEIRA FILHO, J. X. de
700 1 $aMORES, N.
700 1 $aSILVA, M. I. V. da
700 1 $aCÂNDIDO, S. L.
700 1 $aCEZARINO, P. G.
700 1 $aNAKAZATO, L.
700 1 $aDUTRA, V.
773 $tMicrobial Pathogenisis$gv. 122, p. 180-183, 2018.
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