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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Gado de Leite. |
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Data corrente: |
07/07/2017 |
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Data da última atualização: |
30/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
STAFUZZA, N. B.; ZERLOTINI NETO, A.; LOBO, F. P.; YAMAGISHI, M. E. B.; CHUD, T. C. S.; CAETANO, A. R.; MUNARI, D. P.; GARRICK, D. J.; MACHADO, M. A.; MARTINS, M. F.; CARVALHO, M. R.; COLE, J. B.; SILVA, M. V. G. B. |
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Afiliação: |
NEDENIA BONVINO STAFUZZA, FCAV/Unesp; ADHEMAR ZERLOTINI NETO, CNPTIA; FRANCISCO PEREIRA LOBO, CNPTIA; MICHEL EDUARDO BELEZA YAMAGISHI, CNPTIA; TATIANE CRISTINA SELEGUIM CHUD, FCAV/Unesp; ALEXANDRE RODRIGUES CAETANO, Cenargen; DANÍSIO PRADO MUNARI, FCAV/Unesp; DORIAN J. GARRICK, Iowa State University; MARCO ANTONIO MACHADO, CNPGL; MARTA FONSECA MARTINS, CNPGL; MARIA RAQUEL CARVALHO, UFMG; JOHN BRUCE COLE, Agricultural Research Service; MARCOS VINICIUS GUALBERTO B SILVA, CNPGL. |
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Título: |
Single nucleotide variants and InDels identified from whole-genome re-sequencing of Guzerat, Gyr, Girolando and Holstein cattle breeds. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Plos One, v. 12, n. 3, p. 1-15, 2017. |
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DOI: |
https://doi.org/10.1371/journal.pone.0173954 |
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Idioma: |
Inglês |
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Notas: |
Artigo e0173954. Na publicação: Adhemar Zerlotini, Marcos Vinicius Gualberto Barbosa da Silva. |
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Conteúdo: |
Whole-genome re-sequencing, alignment and annotation analyses were undertaken for 12 sires representing four important cattle breeds in Brazil: Guzerat (multi-purpose), Gyr, Girolando and Holstein (dairy production). A total of approximately 4.3 billion reads from an Illumina HiSeq 2000 sequencer generated for each animal 10.7 to 16.4-fold genome coverage. A total of 27,441,279 single nucleotide variations (SNVs) and 3,828,041 insertions/deletions (InDels) were detected in the samples, of which 2,557,670 SNVs and 883,219 InDels were novel. The submission of these genetic variants to the dbSNP database significantly increased the number of known variants, particularly for the indicine genome. The concordance rate between genotypes obtained using the Bovine HD BeadChip array and the same variants identified by sequencing was about 99.05%. The annotation of variants identified numerous non-synonymous SNVs and frameshift InDels which could affect phenotypic variation. Functional enrichment analysis was performed and revealed that variants in the olfactory transduction pathway was over represented in all four cattle breeds, while the ECM-receptor interaction pathway was over represented in Girolando and Guzerat breeds, the ABC transporters pathway was over represented only in Holstein breed, and the metabolic pathways was over represented only in Gyr breed. The genetic variants discovered here provide a rich resource to help identify potential genomic markers and their associated molecular mechanisms that impact economically important traits for Gyr, Girolando, Guzerat and Holstein breeding programs. MenosWhole-genome re-sequencing, alignment and annotation analyses were undertaken for 12 sires representing four important cattle breeds in Brazil: Guzerat (multi-purpose), Gyr, Girolando and Holstein (dairy production). A total of approximately 4.3 billion reads from an Illumina HiSeq 2000 sequencer generated for each animal 10.7 to 16.4-fold genome coverage. A total of 27,441,279 single nucleotide variations (SNVs) and 3,828,041 insertions/deletions (InDels) were detected in the samples, of which 2,557,670 SNVs and 883,219 InDels were novel. The submission of these genetic variants to the dbSNP database significantly increased the number of known variants, particularly for the indicine genome. The concordance rate between genotypes obtained using the Bovine HD BeadChip array and the same variants identified by sequencing was about 99.05%. The annotation of variants identified numerous non-synonymous SNVs and frameshift InDels which could affect phenotypic variation. Functional enrichment analysis was performed and revealed that variants in the olfactory transduction pathway was over represented in all four cattle breeds, while the ECM-receptor interaction pathway was over represented in Girolando and Guzerat breeds, the ABC transporters pathway was over represented only in Holstein breed, and the metabolic pathways was over represented only in Gyr breed. The genetic variants discovered here provide a rich resource to help identify potential genomic markers and their associated... Mostrar Tudo |
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Palavras-Chave: |
Genetic variants; Important traits; Polimorfismo de nucleotídeo único; Potential genomic markers; Single nucleotide variations. |
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Thesagro: |
Gado; Variação genética. |
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Thesaurus Nal: |
Cattle breeds; Genome; Single nucleotide polymorphism. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/171980/1/AP-Single-nucleotide-Stafuzza-etal.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/161515/1/Cnpgl-2017-PlosOne-Stafuzza-Single.pdf
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Marc: |
LEADER 02964naa a2200409 a 4500
001 2086824
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008 2017 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1371/journal.pone.0173954$2DOI
100 1 $aSTAFUZZA, N. B.
245 $aSingle nucleotide variants and InDels identified from whole-genome re-sequencing of Guzerat, Gyr, Girolando and Holstein cattle breeds.$h[electronic resource]
260 $c2017
500 $aArtigo e0173954. Na publicação: Adhemar Zerlotini, Marcos Vinicius Gualberto Barbosa da Silva.
520 $aWhole-genome re-sequencing, alignment and annotation analyses were undertaken for 12 sires representing four important cattle breeds in Brazil: Guzerat (multi-purpose), Gyr, Girolando and Holstein (dairy production). A total of approximately 4.3 billion reads from an Illumina HiSeq 2000 sequencer generated for each animal 10.7 to 16.4-fold genome coverage. A total of 27,441,279 single nucleotide variations (SNVs) and 3,828,041 insertions/deletions (InDels) were detected in the samples, of which 2,557,670 SNVs and 883,219 InDels were novel. The submission of these genetic variants to the dbSNP database significantly increased the number of known variants, particularly for the indicine genome. The concordance rate between genotypes obtained using the Bovine HD BeadChip array and the same variants identified by sequencing was about 99.05%. The annotation of variants identified numerous non-synonymous SNVs and frameshift InDels which could affect phenotypic variation. Functional enrichment analysis was performed and revealed that variants in the olfactory transduction pathway was over represented in all four cattle breeds, while the ECM-receptor interaction pathway was over represented in Girolando and Guzerat breeds, the ABC transporters pathway was over represented only in Holstein breed, and the metabolic pathways was over represented only in Gyr breed. The genetic variants discovered here provide a rich resource to help identify potential genomic markers and their associated molecular mechanisms that impact economically important traits for Gyr, Girolando, Guzerat and Holstein breeding programs.
650 $aCattle breeds
650 $aGenome
650 $aSingle nucleotide polymorphism
650 $aGado
650 $aVariação genética
653 $aGenetic variants
653 $aImportant traits
653 $aPolimorfismo de nucleotídeo único
653 $aPotential genomic markers
653 $aSingle nucleotide variations
700 1 $aZERLOTINI NETO, A.
700 1 $aLOBO, F. P.
700 1 $aYAMAGISHI, M. E. B.
700 1 $aCHUD, T. C. S.
700 1 $aCAETANO, A. R.
700 1 $aMUNARI, D. P.
700 1 $aGARRICK, D. J.
700 1 $aMACHADO, M. A.
700 1 $aMARTINS, M. F.
700 1 $aCARVALHO, M. R.
700 1 $aCOLE, J. B.
700 1 $aSILVA, M. V. G. B.
773 $tPlos One$gv. 12, n. 3, p. 1-15, 2017.
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Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
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| 1. |  | STAFUZZA, N. B.; ZERLOTINI NETO, A.; LOBO, F. P.; YAMAGISHI, M. E. B.; CHUD, T. C. S.; CAETANO, A. R.; MUNARI, D. P.; GARRICK, D. J.; MACHADO, M. A.; MARTINS, M. F.; CARVALHO, M. R.; COLE, J. B.; SILVA, M. V. G. B. Single nucleotide variants and InDels identified from whole-genome re-sequencing of Guzerat, Gyr, Girolando and Holstein cattle breeds. Plos One, v. 12, n. 3, p. 1-15, 2017. Artigo e0173954. Na publicação: Adhemar Zerlotini, Marcos Vinicius Gualberto Barbosa da Silva.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Gado de Leite. |
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| Registros recuperados : 1 | |
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