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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
11/01/2018 |
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Data da última atualização: |
27/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, C. S.; QUINTAO, C. C. R.; FREITAS, C. de; CAMARGO, A. J. R.; SERAPIAO, R. V.; CAMARGO, L. S. de A. |
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Afiliação: |
CLARA SLADE OLIVEIRA, CNPGL; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; CELIO DE FREITAS, CNPGL; Agostinho Jorge dos Reis Camargo, PESAGRO RIO; Raquel Varella Serapião, PESAGRO RIO; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
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Título: |
Post implantation development reveals that biopsy procedure can segregate "healthy" from "unhealthy" bovine embryos and prevent miscarriages. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Animal Reproduction Science, v. 184, p. 51-58, 2017. |
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DOI: |
10.1016/j.anireprosci.2017.06.016 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract Embryo biopsy has been performed in bovine in vivo produced embryos for the last twenty years, but little could be done with few embryonic cells in the past. Recently, advances in single cell analysis enabled a wide range of applications using embryo biopsy, from morphology to genetics analysis and different omics-techniques, which are promising for in vitro-fertilized (IVF) embryos. The aim of this study was to address if biopsy procedure would affect post implantation development of IVF blastocyts. Here we show that blastocyst stage do not affect re-expansion of biopsied embryos (regular blastocyst: 73.7%; expanded blastocyst: 73.1%), but affects (p<0.05) implantation (regular blastocyst: 37.8%, expanded blastocyst: 61.0%), so ideally biopsy should be performed in expanded blastocysts. No detrimental effect of biopsy procedure was detected for post-implantation development (calving rates, Biopsy: 47.1%, Control: 41.9%), and normal calves were born (Birth weight, Biopsy: 32.10±7.20kg; Control: 30.95±5.43kg). Surprisingly, we found interesting results suggesting embryo survival can be increased with aggressive procedures (such as embryo biopsy), and this is highly associated with early pregnancy loss (Biopsy: 0%, Control: 17.4%). This finding also suggests morphological classification of day 7 blastocysts is far from ideal, and supposedly, unhealthy embryos can implant but are bound to miscarriage during the first trimester (non-biopsied embryos). Our results show biopsy procedure is safe for bovine IVF embryos, and shed new light into the importance of conceptus in early pregnancy loss in cattle. MenosAbstract Embryo biopsy has been performed in bovine in vivo produced embryos for the last twenty years, but little could be done with few embryonic cells in the past. Recently, advances in single cell analysis enabled a wide range of applications using embryo biopsy, from morphology to genetics analysis and different omics-techniques, which are promising for in vitro-fertilized (IVF) embryos. The aim of this study was to address if biopsy procedure would affect post implantation development of IVF blastocyts. Here we show that blastocyst stage do not affect re-expansion of biopsied embryos (regular blastocyst: 73.7%; expanded blastocyst: 73.1%), but affects (p<0.05) implantation (regular blastocyst: 37.8%, expanded blastocyst: 61.0%), so ideally biopsy should be performed in expanded blastocysts. No detrimental effect of biopsy procedure was detected for post-implantation development (calving rates, Biopsy: 47.1%, Control: 41.9%), and normal calves were born (Birth weight, Biopsy: 32.10±7.20kg; Control: 30.95±5.43kg). Surprisingly, we found interesting results suggesting embryo survival can be increased with aggressive procedures (such as embryo biopsy), and this is highly associated with early pregnancy loss (Biopsy: 0%, Control: 17.4%). This finding also suggests morphological classification of day 7 blastocysts is far from ideal, and supposedly, unhealthy embryos can implant but are bound to miscarriage during the first trimester (non-biopsied embryos). Our results show b... Mostrar Tudo |
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Palavras-Chave: |
Bovine; Early pregnancy loss; Embryo biopsy; Genomic selection; Post-implantation development. |
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Thesaurus Nal: |
in vitro fertilization. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02511naa a2200265 a 4500
001 2085096
005 2023-01-27
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1016/j.anireprosci.2017.06.016$2DOI
100 1 $aOLIVEIRA, C. S.
245 $aPost implantation development reveals that biopsy procedure can segregate "healthy" from "unhealthy" bovine embryos and prevent miscarriages.$h[electronic resource]
260 $c2017
520 $aAbstract Embryo biopsy has been performed in bovine in vivo produced embryos for the last twenty years, but little could be done with few embryonic cells in the past. Recently, advances in single cell analysis enabled a wide range of applications using embryo biopsy, from morphology to genetics analysis and different omics-techniques, which are promising for in vitro-fertilized (IVF) embryos. The aim of this study was to address if biopsy procedure would affect post implantation development of IVF blastocyts. Here we show that blastocyst stage do not affect re-expansion of biopsied embryos (regular blastocyst: 73.7%; expanded blastocyst: 73.1%), but affects (p<0.05) implantation (regular blastocyst: 37.8%, expanded blastocyst: 61.0%), so ideally biopsy should be performed in expanded blastocysts. No detrimental effect of biopsy procedure was detected for post-implantation development (calving rates, Biopsy: 47.1%, Control: 41.9%), and normal calves were born (Birth weight, Biopsy: 32.10±7.20kg; Control: 30.95±5.43kg). Surprisingly, we found interesting results suggesting embryo survival can be increased with aggressive procedures (such as embryo biopsy), and this is highly associated with early pregnancy loss (Biopsy: 0%, Control: 17.4%). This finding also suggests morphological classification of day 7 blastocysts is far from ideal, and supposedly, unhealthy embryos can implant but are bound to miscarriage during the first trimester (non-biopsied embryos). Our results show biopsy procedure is safe for bovine IVF embryos, and shed new light into the importance of conceptus in early pregnancy loss in cattle.
650 $ain vitro fertilization
653 $aBovine
653 $aEarly pregnancy loss
653 $aEmbryo biopsy
653 $aGenomic selection
653 $aPost-implantation development
700 1 $aQUINTAO, C. C. R.
700 1 $aFREITAS, C. de
700 1 $aCAMARGO, A. J. R.
700 1 $aSERAPIAO, R. V.
700 1 $aCAMARGO, L. S. de A.
773 $tAnimal Reproduction Science$gv. 184, p. 51-58, 2017.
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Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 9 | |
| 3. |  | COSTA, A. T.; NUNES, W. M. de C.; ZANUTTO, C. A.; MÜLLER, G. W. Stability of Citrus tristeza virus protective isolates in field conditions. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 45, n. 7, p. 693-700, jul. 2010 Título em português: Estabilidade de isolados protetores contra Citrus tristeza virus em condições de campo.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 4. | | STUCHI, E. S.; GIRARDI, E. A.; SILVA, S. R.; PAROLIN, L. G.; MÜLLER, G. W.; DONADIO, L. C. Performance of field-selected clones of Pera sweet orange in Northern São Paulo state, Brazil. Citrus Res. Technol., 41, e1061, 2020.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 5. | | COLETTA-FILHO, H. D.; TARGON, M. L. P. N.; TAKITA, M. A.; DE NEGRI, J. D.; POMPEU JUNIOR, J.; MACHADO, M. A.; AMARAL, A. M.; MULLER, G. W. First report of the causal agent of Huanglongbing ("Candidatus Liberibacter asiaticus") in Brazil. Plant Disease, v. 88, n. 12, p. 1382, 2004.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 6. | | SIVIERO, A.; RONCATTO, G.; LESSA, L. S.; OLIVEIRA, T. K. de; CUNHA SOBRINHO, A. P. D.; PASSOS, O. S.; SOARES FILHO, W. dos S.; MULLER, G. W. Porta enxertos de citros para a Amazônia Ocidental. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 20.; ANNUAL MEETING OF THE INTERAMERICAN SOCIETY FOR TROPICAL HORTICULTURE, 54., 2008, Vitória. Frutas para todos : estratégias, tecnologias e visão sustentável. Vitória: INCAPER: Sociedade Brasileira de Fruticultura, 2008. 5 p. 1 DVD.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Acre. |
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| 7. | | SIVIERO, A.; RONCATTO, G.; LESSA, L. S.; OLIVEIRA, T. K. de; CUNHA SOBRINHO, A. P. da; PASSOS, O. S.; SOARES FILHO, W. dos S.; MULLER, G. W. Porta enxertos de citros para a Amazônia ocidental. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 20.; ANNUAL MEETING OF THE INTERAMERICAN SOCIETY FOR TROPICAL HORTICULTURE, 54., 2008, Vitória. Frutas para todos: estratégias, tecnologias e visão sustentável: anais. Vitória: INCAPER: Sociedade Brasileira de Fruticultura, 2008.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 8. | | NOVELLI, V. M.; FREITAS-ASTÚA, J.; RODRIGUES, V.; FERREIRA, D. N. M.; SILVA, L. G.; MÜLLER, G. W.; HILF, M. E.; GOTTWALD, T. R.; MACHADO, M. A. Prevalência de endossimbiontes Cardinium em populações de ácaros Brevipalpus phoenicis de diferentes regiões brasileiras. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 21., 2006, Recife, PE. Entomologia: da academia à transferência de tecnologia: resumos. Recife: SEB: UFRPE, 2006. Não paginado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 9. | | STUCHI, E. S.; GIRARDI, E. A.; SILVA, S. R.; SEMPIONATO, O. R.; PAROLIN, L. G.; MÜLLER, G. W.; DONADIO, L. C. Desempenho de clones pré-imunizados de laranjeiras Valência e Natal no Norte do Estado de São Paulo. Citrus Research. Technology, 41, e1057, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 9 | |
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