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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
11/01/2018 |
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Data da última atualização: |
27/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ASCARI, I. J.; ALVES, N. G.; JASMIN, J.; LIMA, R. R.; QUINTAO, C. C. R.; OBERLENDER, G.; MORAES, E. A.; CAMARGO, L. S. de A. |
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Afiliação: |
I. J. ASCARI, UFLA; N. G. ALVES, UFLA; J. JASMIN, UFRJ; Renato Ribeiro Lima, UFLA; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; G. OBERLENDER, IEF Muzambinho, MG; E. A. MORAES, Universidade Federal do Vale do São Francisco; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
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Título: |
Addition of insulin-like growth factor I to the maturation medium of bovine oocytes subjected to heat shock: effects on the production of reactive oxygen species, mitochondrial activity and oocyte competence. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Domestic Animal Endocrinology, v. 60, p. 50-60, 2017. |
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DOI: |
10.1016/j.domaniend.2017.03.003 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract This study was performed to investigate the effects of insulin-like growth factor-I (IGF-I) addition to in vitro maturation (IVM) medium on apoptosis, mitochondrial membrane potential, ROS production, and developmental competence of bovine oocytes subjected to heat shock. Two temperatures (conventional: 24 h at 38.5C, or heat shock: 12 h at 41C followed by 12 h at 38.5C) and 3 IGF-I concentrations (0, 25, and 100 ng/mL) were tested during IVM. The oocytes were then fertilized in vitro, and the presumptive zygotes were cultured until reaching the blastocyst stage. There was no interaction between temperature and IGF-I concentration for any variable evaluated (P > 0.05). The addition of IGF-I did not alter the proportion of nuclear maturation, TUNEL-positive oocytes and caspase-3 activity, or blastocyst proportion on Days 7 and 8 post-fertilization. Furthermore, the total number of cells and the number of cells in the inner cell mass (ICM) in the blastocyst were not altered (P > 0.05). However, IGF-I increased (P < 0.05) the mitochondrial membrane potential and the production of ROS in oocytes and decreased (P < 0.05) the proportion of apoptotic cells in the ICM in blastocysts. Heat shock increased (P < 0.05) the proportion of TUNEL-positive oocytes and ROS production and reduced (P < 0.05) the mitochondrial membrane potential. Moreover, heat shock increased (P < 0.05) the apoptosis proportion in the ICM cells. In conclusion, supplementing IVM mediumwith IGF-I may increase the mitochondrial membrane potential and ROS production in oocytes and decrease apoptosis in the ICM in blastocysts. Heat shock for 12 h compromised oocyte developmental competence and increased apoptosis within the ICM cells of the blastocysts. MenosAbstract This study was performed to investigate the effects of insulin-like growth factor-I (IGF-I) addition to in vitro maturation (IVM) medium on apoptosis, mitochondrial membrane potential, ROS production, and developmental competence of bovine oocytes subjected to heat shock. Two temperatures (conventional: 24 h at 38.5C, or heat shock: 12 h at 41C followed by 12 h at 38.5C) and 3 IGF-I concentrations (0, 25, and 100 ng/mL) were tested during IVM. The oocytes were then fertilized in vitro, and the presumptive zygotes were cultured until reaching the blastocyst stage. There was no interaction between temperature and IGF-I concentration for any variable evaluated (P > 0.05). The addition of IGF-I did not alter the proportion of nuclear maturation, TUNEL-positive oocytes and caspase-3 activity, or blastocyst proportion on Days 7 and 8 post-fertilization. Furthermore, the total number of cells and the number of cells in the inner cell mass (ICM) in the blastocyst were not altered (P > 0.05). However, IGF-I increased (P < 0.05) the mitochondrial membrane potential and the production of ROS in oocytes and decreased (P < 0.05) the proportion of apoptotic cells in the ICM in blastocysts. Heat shock increased (P < 0.05) the proportion of TUNEL-positive oocytes and ROS production and reduced (P < 0.05) the mitochondrial membrane potential. Moreover, heat shock increased (P < 0.05) the apoptosis proportion in the ICM cells. In conclusion, supplementing IVM mediumwith IGF-I may inc... Mostrar Tudo |
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Palavras-Chave: |
Mitochondrial membrane potential; ROS production; Thermoprotection. |
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Thesaurus Nal: |
DNA fragmentation; heat stress. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02716naa a2200277 a 4500
001 2085044
005 2023-01-27
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1016/j.domaniend.2017.03.003$2DOI
100 1 $aASCARI, I. J.
245 $aAddition of insulin-like growth factor I to the maturation medium of bovine oocytes subjected to heat shock$beffects on the production of reactive oxygen species, mitochondrial activity and oocyte competence.$h[electronic resource]
260 $c2017
520 $aAbstract This study was performed to investigate the effects of insulin-like growth factor-I (IGF-I) addition to in vitro maturation (IVM) medium on apoptosis, mitochondrial membrane potential, ROS production, and developmental competence of bovine oocytes subjected to heat shock. Two temperatures (conventional: 24 h at 38.5C, or heat shock: 12 h at 41C followed by 12 h at 38.5C) and 3 IGF-I concentrations (0, 25, and 100 ng/mL) were tested during IVM. The oocytes were then fertilized in vitro, and the presumptive zygotes were cultured until reaching the blastocyst stage. There was no interaction between temperature and IGF-I concentration for any variable evaluated (P > 0.05). The addition of IGF-I did not alter the proportion of nuclear maturation, TUNEL-positive oocytes and caspase-3 activity, or blastocyst proportion on Days 7 and 8 post-fertilization. Furthermore, the total number of cells and the number of cells in the inner cell mass (ICM) in the blastocyst were not altered (P > 0.05). However, IGF-I increased (P < 0.05) the mitochondrial membrane potential and the production of ROS in oocytes and decreased (P < 0.05) the proportion of apoptotic cells in the ICM in blastocysts. Heat shock increased (P < 0.05) the proportion of TUNEL-positive oocytes and ROS production and reduced (P < 0.05) the mitochondrial membrane potential. Moreover, heat shock increased (P < 0.05) the apoptosis proportion in the ICM cells. In conclusion, supplementing IVM mediumwith IGF-I may increase the mitochondrial membrane potential and ROS production in oocytes and decrease apoptosis in the ICM in blastocysts. Heat shock for 12 h compromised oocyte developmental competence and increased apoptosis within the ICM cells of the blastocysts.
650 $aDNA fragmentation
650 $aheat stress
653 $aMitochondrial membrane potential
653 $aROS production
653 $aThermoprotection
700 1 $aALVES, N. G.
700 1 $aJASMIN, J.
700 1 $aLIMA, R. R.
700 1 $aQUINTAO, C. C. R.
700 1 $aOBERLENDER, G.
700 1 $aMORAES, E. A.
700 1 $aCAMARGO, L. S. de A.
773 $tDomestic Animal Endocrinology$gv. 60, p. 50-60, 2017.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 10 | |
| 1. |  | SENABIO, J. A.; PEREIRA, F. de C.; PIETRO-SOUZA, W.; SOUSA, T. F.; SILVA, G. F. da; SOARES, M. A. Enhanced mercury phytoremediation by Pseudomonodictys pantanalensis sp. nov. A73 and Westerdykella aquatica P71. Brazilian Journal of Microbiology, v. 54, n. 2, p. 949-964, jun. 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 2. | | MELLO, I. S.; PIETRO-SOUZA, W.; BARROS, B. M.; SILVA, G. F. da; CAMPOS, M. L.; SOARES, M. A. Endophytic bacteria mitigate mercury toxicity to host plants. Symbiosis, v. 79, n. 3, p 251-262, Nov. 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 3. | | SILVA, F. A.; LIOTTI, R. G.; PIETRO-SOUZA, W.; SILVA, G. F.; SOARES, M. A.; BICHARA, K. P. A. Endophytic fungi of Paullinia cupana and its antimicrobial potential. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., 2015, Florianópolis. Anais... São Paulo: Sociedade Brasileira de Microbiologia, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 4. | | MELLO, I. S.; PIETRO-SOUZA, W.; LIOTTI, R. G.; PEREIRA, J. M. C.; BICHARA, K. P. A.; SILVA, G. F.; ALMEIDA, E. G.; SOARES, M. A. Endophytic bacterium isolated of hosts present in contaminated areas by mercury in Pantanal mato-grossense. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., 2015, Florianópolis. Anais... São Paulo: Sociedade Brasileira de Microbiologia, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 5. | | PIETRO-SOUZA, W.; MELLO, I. S.; VENDRUSCULLO, S. J.; SILVA, G. F. da; CUNHA, C. N. da; WHITE, J. F.; SOARES, M. A. Endophytic fungal communities of Polygonum acuminatum and Aeschynomene fluminensis are influenced by soil mercury contamination. Plos One, v. 12, n. 7, p. e0182017, July 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 6. | | MELLO, I. S.; PIETRO-SOUZA, W.; LIOTTI, R. G.; BARROS, B. M.; BICHARA, K. P. A.; SILVA, G. F.; ALMEIDA, E. G.; SOARES, M. A. Resistante to mercury of endophytic bacteria obtained in contaminated sites. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., 2015, Florianópolis. Anais... São Paulo: Sociedade Brasileira de Microbiologia, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 7. | | LIOTTI, R. G.; MELLO, I. S.; PIETRO-SOUZA, W.; FIGUEIREDO, M. I. S.; SILVEIRA, B. A. V.; SILVA, F. A.; BICHARA, K. P. A.; ALMEIDA, E. G.; SILVA, G. F.; SOARES, M. A. Antibiosis of actinomycetes from Paullina cupana var. sorbilis (mart.) Ducke against fungi pathogenic. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., 2015, Florianópolis. Anais... São Paulo: Sociedade Brasileira de Microbiologia, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 8. | | PIETRO-SOUZA, W.; VENDRUSCULLO, S. J.; MELLO, I. S.; LIOTTI, R. G.; BICHARA, K. P. A.; SILVA, G. F.; ALMEIDA, E. G.; NUNES DA CUNHA, C.; SOARES, M. A. Capacity resistance of endophytic fungi the mercury. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., 2015, Florianópolis. Anais... São Paulo: Sociedade Brasileira de Microbiologia, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 9. | | PIETRO-SOUZA, W.; VENDRUSCULLO, S. J.; MELLO, I. S.; LIOTTI, R. G.; BICHARA, K. P. A.; SILVA, G. F.; ALMEIDA, E. G.; NUNES DA CUNHA, C.; SOARES, M. A. Isolation of endophytic fungi in environments with and without traces of mercury contamination, Pantanal of Mato Grosso. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., 2015, Florianópolis. Anais... São Paulo: Sociedade Brasileira de Microbiologia, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 10. | | LIOTTI, R. G.; MELLO, I. S.; PIETRO-SOUZA, W.; FIGUEIREDO, M. I. S.; SILVEIRA, B. A. V.; SILVA, F. A.; BICHARA, K. P. de A.; ALMEIDA, E. G.; SILVA, G. F. da; SOARES, M. A. Microbiol diversity in rhizosphere resistant and susceptible guarana collected in Maués and Manaus. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., 2015, Florianópolis. Anais... São Paulo: Sociedade Brasileira de Microbiologia, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| Registros recuperados : 10 | |
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