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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
10/01/2018 |
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Data da última atualização: |
27/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ASSIS, A. S. F.; OTENIO, M. H.; DRUMOND, B. P.; FUMIAN, T. M.; MIAGOSTOVICH, M. P.; SILVA, M. L. da R. e. |
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Afiliação: |
Andrêssa S.F.Assis, UFJF; MARCELO HENRIQUE OTENIO, CNPGL; Betânia P. Drumond, UFJF; Tulio M. Fumian, Instituto Oswaldo Cruz; Marize P Miagostovich, Instituto Oswaldo Cruz; Maria Luzia da Rosa e Silva, UFJF. |
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Título: |
Optimization of the skimmed-milk flocculation method for recovery of adenovirus from sludge. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Science of The Total Environment, v. 583, p. 163-168, 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract Return of treated sludge to the environment poses concerns and has stimulated the development of studies on viral monitoring in this matrix, in order to assess its potential risks for public health. Human adenovirus (HAdV) has been identified as a putative viral marker of faecal contamination due to its stability and resistance to the sewage treatment process. The aim of this study was to optimize the organic flocculation procedure in order to establish an appropriate methodology for HAdV recovery from sewage sludge samples. Four protocols (A-D) have been proposed, with changes in the initial sample dilution, in the stirring time and in the final concentration of skimmed-milk. A single sludge sample was obtained in Wastewater Treatment Plant (WWTP) and divided into aliquots. In each protocol, three aliquots were inoculated with HAdV and bacteriophage PP7 and a non-inoculated one was used as negative control. Viral load and recovery rate were determined by quantitative PCR. HAdV recovery rate varied between the protocols tested (p=0.016) and the best result was obtained through the protocol C. In order to confirm this result a field study with activated, thickened and digested sludge sampleswas carried out. Different types of sludgewere obtained in twoWWTPs and processed using protocol C. HAdVwas detected in all samples, with a similar or higher viral load than those obtainedwith other concentration techniques already applied to sludge. Protocol C proved to be really efficient, with the advantage of showing low cost and practicability in routine laboratories. MenosAbstract Return of treated sludge to the environment poses concerns and has stimulated the development of studies on viral monitoring in this matrix, in order to assess its potential risks for public health. Human adenovirus (HAdV) has been identified as a putative viral marker of faecal contamination due to its stability and resistance to the sewage treatment process. The aim of this study was to optimize the organic flocculation procedure in order to establish an appropriate methodology for HAdV recovery from sewage sludge samples. Four protocols (A-D) have been proposed, with changes in the initial sample dilution, in the stirring time and in the final concentration of skimmed-milk. A single sludge sample was obtained in Wastewater Treatment Plant (WWTP) and divided into aliquots. In each protocol, three aliquots were inoculated with HAdV and bacteriophage PP7 and a non-inoculated one was used as negative control. Viral load and recovery rate were determined by quantitative PCR. HAdV recovery rate varied between the protocols tested (p=0.016) and the best result was obtained through the protocol C. In order to confirm this result a field study with activated, thickened and digested sludge sampleswas carried out. Different types of sludgewere obtained in twoWWTPs and processed using protocol C. HAdVwas detected in all samples, with a similar or higher viral load than those obtainedwith other concentration techniques already applied to sludge. Protocol C proved to be really... Mostrar Tudo |
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Palavras-Chave: |
Human adenovirus; Internal control; Organic flocculation; Quantitative PCR; Wastewater treatment plant. |
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Thesaurus Nal: |
sewage sludge. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02366naa a2200253 a 4500
001 2084982
005 2023-01-27
008 2017 bl uuuu u00u1 u #d
100 1 $aASSIS, A. S. F.
245 $aOptimization of the skimmed-milk flocculation method for recovery of adenovirus from sludge.$h[electronic resource]
260 $c2017
520 $aAbstract Return of treated sludge to the environment poses concerns and has stimulated the development of studies on viral monitoring in this matrix, in order to assess its potential risks for public health. Human adenovirus (HAdV) has been identified as a putative viral marker of faecal contamination due to its stability and resistance to the sewage treatment process. The aim of this study was to optimize the organic flocculation procedure in order to establish an appropriate methodology for HAdV recovery from sewage sludge samples. Four protocols (A-D) have been proposed, with changes in the initial sample dilution, in the stirring time and in the final concentration of skimmed-milk. A single sludge sample was obtained in Wastewater Treatment Plant (WWTP) and divided into aliquots. In each protocol, three aliquots were inoculated with HAdV and bacteriophage PP7 and a non-inoculated one was used as negative control. Viral load and recovery rate were determined by quantitative PCR. HAdV recovery rate varied between the protocols tested (p=0.016) and the best result was obtained through the protocol C. In order to confirm this result a field study with activated, thickened and digested sludge sampleswas carried out. Different types of sludgewere obtained in twoWWTPs and processed using protocol C. HAdVwas detected in all samples, with a similar or higher viral load than those obtainedwith other concentration techniques already applied to sludge. Protocol C proved to be really efficient, with the advantage of showing low cost and practicability in routine laboratories.
650 $asewage sludge
653 $aHuman adenovirus
653 $aInternal control
653 $aOrganic flocculation
653 $aQuantitative PCR
653 $aWastewater treatment plant
700 1 $aOTENIO, M. H.
700 1 $aDRUMOND, B. P.
700 1 $aFUMIAN, T. M.
700 1 $aMIAGOSTOVICH, M. P.
700 1 $aSILVA, M. L. da R. e
773 $tScience of The Total Environment$gv. 583, p. 163-168, 2017.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 14 | |
| 3. |  | MELLO, E. B.; MATIAS, R.; JANK, L.; OLIVEIRA, N. Caracterização morfológica e diversidade genética de híbridos de Panicum maximum Jacq. In: JORNADA CIENTÍFICA DA EMBRAPA GADO DE CORTE, 6., 2010, Campo Grande, MS. Ética na pesquisa científica: [Anais da ...]. Campo Grande, MS: Embrapa Gado de Corte, 2010. 1 CD-ROM. Coordenadora: Vanessa Felipe de Souza 1 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 4. | | MATIAS, R.; SOLOM, S.; RESENDE, U. M.; GOMES, A.; KOLLER, W. V. Melia azedarach uso popular x estudos químicos e farmacológico. Ensaios e Ciência, Campo Grande, v. 6, n. 1, p. 91-121, abr. 2002. CNPGC.| Biblioteca(s): Embrapa Gado de Corte. |
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| 5. | | PISTORI, M. G. B.; ROEL, A. R.; VALERIO, J. R.; OLIVEIRA, M. C. M.; GRISOTO, E.; MATIAS. R. Effect of Anacardium humile St. Hill (Anacardiaceae) Aqueous Extract on Mahanarva fimbriolata (Stal, 1854) (Hemiptera: Cercopidae). Acta Scientiarum. Agronomy. Maringá, v. 35, n. 4, p. 413-417, Oct.-Dec., 2013| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 6. | | MATIAS, R.; SOLON, S.; GOMES, A.; MAGANHA, M.; PEREIRA, G. da S.; NOZU, P.; KOLLER, W. W. Estudo químico-farmacológico de Melia azedarach (meliaceae) sobre Boophilus microplus. Ensaios e Ciência, Campo Grande-MS, v.7, n.2, p.283-298, ago.2003.| Biblioteca(s): Embrapa Gado de Corte. |
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| 10. | | DANTAS, D. A.; MAGANHA, M.; BERETTA, T. E.; NOZU, P.; PEREIRA, G. da S.; MATIAS, R.; SOLON, S.; RESENDE, U.; KOLLER, W. W.; GOMES, A. Estudo fitoquímico dos frutos de Melia azedarach L. (Cinamomo, Meliaceae). In: ENCONTRO DE PESQUISA E INICIAÇÃO CIENTÍFICA DA UNIDERP, 2., 2000, Campo Grande. Anais... Campo Grande: Ed. UNIDERP, 2000. p. 119-120. CNPGC.| Biblioteca(s): Embrapa Gado de Corte. |
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| 11. | | PEDRINHO, D. R.; FERREIRA, A. D.; LAURA, V. A.; BONO, J. A. M.; PEREIRA, S. R.; MARQUES, S. P. de M.; MATIAS, R.; NASCIMENTO, L. S. do. Biometria de genótipos de eucalipto cultivados em solo arenoso na Região Central de Mato Grosso do Sul. Campo Grande, MS: Embrapa Gado de Corte, 2019. 24 p. Embrapa Gado de Corte. Documentos, 265).| Tipo: Autoria/Organização/Edição de Livros |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 12. | | ALVES, B. G.; MATIAS, R. R.; ALBUQUERQUE, J. M. da R.; RODRIGUES, A. de M.; ROCHA, F. A. da S.; SOUSA, T. F.; SILVA, G. F. da; KOOLEN, H. H. F. Estudo microbiológico e avaliação do potencial bioativo da família Hypocreaceae. In: CONGRESSO BRASILEIRO DE MICOLOGIA, 9., 2019, Manaus. Anais... Manaus: Inpa, 2019. p. 74.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 13. | | RODRIGUES, M. I.; SOUZA, A. N. de; FREITAS, L. J. M. de; SILVA, J. N. M.; PEREIRA, R. S.; BIALI, L. J.; INKOTTE, J.; ARAÚJO, J. B. C. N.; MATIAS, R. A. M. Case study on the financial viability of forest management on public lands in the Brazilian Amazon. Forests, v. 14, n. 12, 2309, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 14. | | PEREIRA, D. I. de M.; GURGEL, R. S.; SOUZA, A. T. F. de; MATIAS, R. R.; FALCÃO, L. de S.; CHAVES, F. C. M.; SILVA, G. F. da; MARTÍNEZ, J. G.; PROCÓPIO, R. E. de L.; FANTIN, C.; ALBUQUERQUE, P. M. Isolation and identification of pigment-producing endophytic fungi from the Amazonian species Fridericia chica. Journal of Fungi, v. 10, n. 1, art. 77, 2024.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| Registros recuperados : 14 | |
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