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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com rosangela.lacerda@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
10/01/2018 |
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Data da última atualização: |
27/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ASSIS, A. S. F.; OTENIO, M. H.; DRUMOND, B. P.; FUMIAN, T. M.; MIAGOSTOVICH, M. P.; SILVA, M. L. da R. e. |
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Afiliação: |
Andrêssa S.F.Assis, UFJF; MARCELO HENRIQUE OTENIO, CNPGL; Betânia P. Drumond, UFJF; Tulio M. Fumian, Instituto Oswaldo Cruz; Marize P Miagostovich, Instituto Oswaldo Cruz; Maria Luzia da Rosa e Silva, UFJF. |
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Título: |
Optimization of the skimmed-milk flocculation method for recovery of adenovirus from sludge. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Science of The Total Environment, v. 583, p. 163-168, 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract Return of treated sludge to the environment poses concerns and has stimulated the development of studies on viral monitoring in this matrix, in order to assess its potential risks for public health. Human adenovirus (HAdV) has been identified as a putative viral marker of faecal contamination due to its stability and resistance to the sewage treatment process. The aim of this study was to optimize the organic flocculation procedure in order to establish an appropriate methodology for HAdV recovery from sewage sludge samples. Four protocols (A-D) have been proposed, with changes in the initial sample dilution, in the stirring time and in the final concentration of skimmed-milk. A single sludge sample was obtained in Wastewater Treatment Plant (WWTP) and divided into aliquots. In each protocol, three aliquots were inoculated with HAdV and bacteriophage PP7 and a non-inoculated one was used as negative control. Viral load and recovery rate were determined by quantitative PCR. HAdV recovery rate varied between the protocols tested (p=0.016) and the best result was obtained through the protocol C. In order to confirm this result a field study with activated, thickened and digested sludge sampleswas carried out. Different types of sludgewere obtained in twoWWTPs and processed using protocol C. HAdVwas detected in all samples, with a similar or higher viral load than those obtainedwith other concentration techniques already applied to sludge. Protocol C proved to be really efficient, with the advantage of showing low cost and practicability in routine laboratories. MenosAbstract Return of treated sludge to the environment poses concerns and has stimulated the development of studies on viral monitoring in this matrix, in order to assess its potential risks for public health. Human adenovirus (HAdV) has been identified as a putative viral marker of faecal contamination due to its stability and resistance to the sewage treatment process. The aim of this study was to optimize the organic flocculation procedure in order to establish an appropriate methodology for HAdV recovery from sewage sludge samples. Four protocols (A-D) have been proposed, with changes in the initial sample dilution, in the stirring time and in the final concentration of skimmed-milk. A single sludge sample was obtained in Wastewater Treatment Plant (WWTP) and divided into aliquots. In each protocol, three aliquots were inoculated with HAdV and bacteriophage PP7 and a non-inoculated one was used as negative control. Viral load and recovery rate were determined by quantitative PCR. HAdV recovery rate varied between the protocols tested (p=0.016) and the best result was obtained through the protocol C. In order to confirm this result a field study with activated, thickened and digested sludge sampleswas carried out. Different types of sludgewere obtained in twoWWTPs and processed using protocol C. HAdVwas detected in all samples, with a similar or higher viral load than those obtainedwith other concentration techniques already applied to sludge. Protocol C proved to be really... Mostrar Tudo |
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Palavras-Chave: |
Human adenovirus; Internal control; Organic flocculation; Quantitative PCR; Wastewater treatment plant. |
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Thesaurus Nal: |
sewage sludge. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02366naa a2200253 a 4500
001 2084982
005 2023-01-27
008 2017 bl uuuu u00u1 u #d
100 1 $aASSIS, A. S. F.
245 $aOptimization of the skimmed-milk flocculation method for recovery of adenovirus from sludge.$h[electronic resource]
260 $c2017
520 $aAbstract Return of treated sludge to the environment poses concerns and has stimulated the development of studies on viral monitoring in this matrix, in order to assess its potential risks for public health. Human adenovirus (HAdV) has been identified as a putative viral marker of faecal contamination due to its stability and resistance to the sewage treatment process. The aim of this study was to optimize the organic flocculation procedure in order to establish an appropriate methodology for HAdV recovery from sewage sludge samples. Four protocols (A-D) have been proposed, with changes in the initial sample dilution, in the stirring time and in the final concentration of skimmed-milk. A single sludge sample was obtained in Wastewater Treatment Plant (WWTP) and divided into aliquots. In each protocol, three aliquots were inoculated with HAdV and bacteriophage PP7 and a non-inoculated one was used as negative control. Viral load and recovery rate were determined by quantitative PCR. HAdV recovery rate varied between the protocols tested (p=0.016) and the best result was obtained through the protocol C. In order to confirm this result a field study with activated, thickened and digested sludge sampleswas carried out. Different types of sludgewere obtained in twoWWTPs and processed using protocol C. HAdVwas detected in all samples, with a similar or higher viral load than those obtainedwith other concentration techniques already applied to sludge. Protocol C proved to be really efficient, with the advantage of showing low cost and practicability in routine laboratories.
650 $asewage sludge
653 $aHuman adenovirus
653 $aInternal control
653 $aOrganic flocculation
653 $aQuantitative PCR
653 $aWastewater treatment plant
700 1 $aOTENIO, M. H.
700 1 $aDRUMOND, B. P.
700 1 $aFUMIAN, T. M.
700 1 $aMIAGOSTOVICH, M. P.
700 1 $aSILVA, M. L. da R. e
773 $tScience of The Total Environment$gv. 583, p. 163-168, 2017.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 66 | |
| 2. |  | BASTIANI, M. O.; LAMEGO, F. P.; CARATTI, F, C.; ROCKENBACH, D.; BALBINOT, A.; SOUZA, G. M. Competition between the exotic grass Eragrostis plana and forage grass Paspalum nutatum under drought conditions. In: WEED SCIENCE SOCIETY OF AMERICA ANNUAL MEETING, 2018, Arlington. Proceedings... Arlington: WSSA, 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 8. | | FAUSTO, D. A.; REZENDE, M. A.; SOUZA, G. M.; FERRAZ, A. J.; DELGADO, E. F.; FEIJO, G. L. D. Efeito do ganho compensatório nas características de carcaça de fêmeas zebuínas adultas. In: JORNADA CIENTÍFICA DA EMBRAPA GADO DE CORTE, 6., 2010, Campo Grande, MS. Ética na pesquisa científica: [Anais da ...]. Campo Grande, MS: Embrapa Gado de Corte, 2010. 1 CD-ROM. Coordenadora: Vanessa Felipe de Souza 1 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 9. | | PEREIRA, H. L.; REZENDE, M. A.; SOUZA, G. M.; FAUSTO, D. A.; TEIXEIRA, F. M. C.; FEIJO, G. L. D. Efeito da maturação na qualidade da carne de vacas de descarte. In: JORNADA CIENTÍFICA DA EMBRAPA GADO DE CORTE, 6., 2010, Campo Grande, MS. Ética na pesquisa científica: [Anais da ...]. Campo Grande, MS: Embrapa Gado de Corte, 2010. 1 CD-ROM. Coordenadora: Vanessa Felipe de Souza 1 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 10. | | BASTIANI, M. O.; LAMEGO, F. P.; BENEDETTI, L.; CARATTI, F. C.; SOUZA, G. M.; ROMA-BURGOS, N. Elevated CO2 effect on tolerance of Eragrostis plana to glyphosate and drought stress. In: SOUTHERN WEED SCIENCE SOCIETY ANNUAL MEETING, 71., 2018, Atlanta. Proceedings... Atlanta: SWSS, 2018. p. 129.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 11. | | SURITA, L. M. A.; BONIN, M. N.; FERRAZ, A. L. J.; SOUZA, G. M.; FEIJO, G. L. D. Correlação entre medidas de qualidade de carne realizadas na altura da 5ª e 12ª costelas de bovinos cruzados. In: JORNADA CIENTÍFICA EMBRAPA GADO DE CORTE, 9., 2013, Campo Grande, MS. [Anais da..]. Campo Grande, MS: Embrapa Gado de Corte, 2013. (Embrapa Gado de Corte. Documentos, 204). Comissão organizadora: Denise Baptaglin Montagner, Grácia Maria Soares Rosinha, Rodrigo Carvalho Alva.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 12. | | CATUCHI, T. A.; GUIDORIZZI, F. V. C.; GUIDORIZI, K. A.; BARBOSA, A. de M. B.; SOUZA, G. M. Respostas fisiológicas de cultivares de soja à adubação potássica sob diferentes regimes hídricos. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 47, n. 4, p. 519-527, abr. 2012. Tíitulo em inglês: Physiological responses of soybean cultivars to potassium fertilization under different water regimes.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 13. | | TEIXEIRA, F. M. C.; FAUSTO, D. A.; REZENDE, M. A.; SOUZA, G. M.; PEREIRA, H. L.; FEIJO, G. L. D. Relação de medidas do músculo Longissimus dorsi com a maciez da carne de vacas de descarte. In: JORNADA CIENTÍFICA DA EMBRAPA GADO DE CORTE, 6., 2010, Campo Grande, MS. Ética na pesquisa científica: [Anais da ...]. Campo Grande, MS: Embrapa Gado de Corte, 2010. 1 CD-ROM. Coordenadora: Vanessa Felipe de Souza 1 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 14. | | FAUSTO, D. A.; REZENDE, M. A.; FERRAZ, A. L. J; SOUZA, G. M; DELGADO, E. F; FEIJO, G. L. D. Changes in the carcass characteristics of zebu cows under different feeding management systems. In: INTERNATIONAL CONGRESS OF MEAT SCIENCE AND TECHNOLOGY, 59., 2013, Izmir. Proceedings... Izmir: ICoMST, 2013. 3 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 16. | | MORDESTO, G. B. A.; MOREIRA, A. N.; OLIVEIRA, J. E. de M.; SOUZA, G. M. de; OLIVEIRA, A. C.; SOUZA, I. D. de. Influência da cor e altura de armadilhas adesivas na incidência de tripes na cultura da videira. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 23., 2010, Natal. Anais... Natal: Sociedade Brasileira de Entomologia, 2010. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 17. | | AMANDO, M. L. C. S.; OLIVEIRA, J. E. de M.; SOUZA, G. M. M. de; MOREIRA, A. N.; SILVA, R. R. S. da. Indicadores de racionalização de agrotóxicos em áreas produtoras de uva no Vale do São Francisco. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 21., 2010, Natal. Frutas: saúde, inovação e responsabilidade: anais. Natal: SBF, 2010. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 18. | | PFEIFFER, M. A.; WOUDSTRA, S.; TEIXEIRA, V. A.; KNEGT, L. V. de; SOUZA, G. M. de; TOMICH, T. R.; CAMPOS, M. M.; PEREIRA, L. G. R. Evaluating sensor-derived rumination and activity data for monitoring pre-weaning dairy calf: a correlation study with traditional metrics. In: ANNUAL MEETING OF THE EUROPEAN FEDERATION ON ANIMAL SCIENCE, 76., 2025, Innsbruck, Austria. Book of abstracts. Roma: European Federation of Animal Science, 2025. p. 547.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 19. | | RIBEIRO, R. V.; SANTOS, M. G. dos; SOUZA, G. M.; MACHADO, E. C.; OLIVEIRA, R. F. de; ANGELOCCI, L. R.; PIMENTEL, C. Environmental effects on photosynthetic capacity of bean genotypes. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 39, n. 7, p. 615-623, jul. 2004 Título em português: Efeitos ambientais na capacidade fotossintética de genótipos de feijoeiro.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 20. | | SOUZA, G. M. M. de; OLIVEIRA, J. E. de M.; MOREIRA, A. N.; AMANDO, M. L. C. S.; SANTOS, A. C.; SOUZA, I. D. de; OLIVEIRA, A. C. Influência de variedades tardias de uvas viníferas na dinâmica populacional da traça-dos-cachos a Cryptoblabes gnidiella (Millière) (Lepidoptera, Pyralidae). In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 23., 2010, Natal. Anais... Natal: Sociedade Brasileira de Entomologia, 2010. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| Registros recuperados : 66 | |
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