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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
10/11/2017 |
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Data da última atualização: |
13/11/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GALVÃO, C. E.; FRAGOSO, S. P.; OLIVEIRA, C. E. de; FORNER, O.; PEREIRA, R. R. B.; SOARES, C. O.; ROSINHA, G. M. S. |
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Afiliação: |
CLEBER EDUARDO GALVÃO, Federal University of Mato Grosso do Sul (UFMS), Campo Grande, MS.; STENIO PERDIGÃO FRAGOSO, Carlos Chagas Institute, Oswaldo Cruz Foundation (ICC-FIOCRUZ), Curitiba, PR.; CARINA ELISEI DE OLIVEIRA, Dom Bosco Catholic University (UCDB), Campo Grande, MS.; ODINÉIA FORNER, Federal University of Paraná (UFPR), Curitiba, PR.; RENATA RIBEIRO BASTOS PEREIRA, Federal University of Mato Grosso do Sul (UFMS), Campo Grande, MS.; CLEBER OLIVEIRA SOARES, DE/TT; GRACIA MARIA SOARES ROSINHA, CNPGC. |
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Título: |
Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
BMC Microbiology, v. 17, n. 202, p. 1-8, sept., 2017. |
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DOI: |
10.1186/s12866-017-1110-7 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Caseous lymphadenitis (CLA) is a disease that affects sheep, goats and occasionally humans. The etiologic agent is the Corynebacterium pseudotuberculosis bacillus. The objective of this study was to build a gene expression library from C. pseudotuberculosis and use immunoscreening to identify genes that encode potential antigenic proteins for the development of DNA and subunit vaccines against CLA. Results: A wild strain of C. pseudotuberculosis was used for extraction and partial digestion of genomic DNA. Sequences between 1000 and 5000 base pairs (bp) were excised from the gel, purified, and the digested DNA fragments were joined to bacteriophage vector ZAP Express, packaged into phage and transfected into Escherichia coli . For immunoscreening a positive sheep sera pool and a negative sera pool for CLA were used. Four clones were identified that strongly reacted to sera. The clones were confirmed by polymerase chain reaction (PCR) followed by sequencing for genomic comparison of C. pseudotuberculosis in GenBank. The genes identified were dak2, fagA, fagB, NlpC/P60 protein family and LPxTG putative protein family. Conclusion: Proteins of this type can be antigenic which could aid in the development of subunit or DNA vaccines against CLA as well as in the development of serological tests for diagnosis. Immunoscreening of the gene expression library was shown to be a sensitive and efficient technique to identify probable immunodominant genes. |
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Palavras-Chave: |
DNA vaccines. |
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Thesagro: |
Corynebacterium Pseudotuberculosis. |
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Thesaurus Nal: |
Caseous lymphadenitis; Gene expression; Sheep. |
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Categoria do assunto: |
-- |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/1079448/1/IdentificationofnewCorynebacterium.pdf
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Marc: |
LEADER 02301naa a2200265 a 4500
001 2079448
005 2017-11-13
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1186/s12866-017-1110-7$2DOI
100 1 $aGALVÃO, C. E.
245 $aIdentification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library.$h[electronic resource]
260 $c2017
520 $aBackground: Caseous lymphadenitis (CLA) is a disease that affects sheep, goats and occasionally humans. The etiologic agent is the Corynebacterium pseudotuberculosis bacillus. The objective of this study was to build a gene expression library from C. pseudotuberculosis and use immunoscreening to identify genes that encode potential antigenic proteins for the development of DNA and subunit vaccines against CLA. Results: A wild strain of C. pseudotuberculosis was used for extraction and partial digestion of genomic DNA. Sequences between 1000 and 5000 base pairs (bp) were excised from the gel, purified, and the digested DNA fragments were joined to bacteriophage vector ZAP Express, packaged into phage and transfected into Escherichia coli . For immunoscreening a positive sheep sera pool and a negative sera pool for CLA were used. Four clones were identified that strongly reacted to sera. The clones were confirmed by polymerase chain reaction (PCR) followed by sequencing for genomic comparison of C. pseudotuberculosis in GenBank. The genes identified were dak2, fagA, fagB, NlpC/P60 protein family and LPxTG putative protein family. Conclusion: Proteins of this type can be antigenic which could aid in the development of subunit or DNA vaccines against CLA as well as in the development of serological tests for diagnosis. Immunoscreening of the gene expression library was shown to be a sensitive and efficient technique to identify probable immunodominant genes.
650 $aCaseous lymphadenitis
650 $aGene expression
650 $aSheep
650 $aCorynebacterium Pseudotuberculosis
653 $aDNA vaccines
700 1 $aFRAGOSO, S. P.
700 1 $aOLIVEIRA, C. E. de
700 1 $aFORNER, O.
700 1 $aPEREIRA, R. R. B.
700 1 $aSOARES, C. O.
700 1 $aROSINHA, G. M. S.
773 $tBMC Microbiology$gv. 17, n. 202, p. 1-8, sept., 2017.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Pesca e Aquicultura. |
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Data corrente: |
30/10/2012 |
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Data da última atualização: |
09/11/2012 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
AQUINO, R. F.; SILVA, M. L. N.; FREITAS, D. A. F. de; CURI, N.; MELLO, C. R. de; AVANZI, J. C. |
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Afiliação: |
REGIMEIRE FREITAS AQUINO, INSTITUTO FEDERAL DE EDUCAÇÃO-São João Del Rei, MG; MARX LEANDRO NAVES SILVA, UNIVERSIDADE FEDERAL DE LAVRAS; DIEGO ANTONIO FRANÇA DE FREITAS, UNIVERSIDADE FEDERAL DE LAVRAS; NILTON CURI, UNIVERSIDADE FEDERAL DE LAVRAS; CARLOS ROGÉRIO DE MELLO, UNIVERSIDADE FEDERAL DE LAVRAS; JUNIOR CESAR AVANZI, CNPASA. |
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Título: |
Spatial variability of the rainfall erosivity in southern region of Minas Gerais State, Brazil. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Ciência e Agrotecnologia, Lavras, v. 36, n. 5, p. 533-542, set./out. 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
Rainfall erosivity and its spatial variability were studied for 54 pluviometric stations in Southern Minas Gerais State (48º00? - 44º00?W; 23º50? - 20º00?S), aiming to plan the land-use strategies. Therefore, erosivity factor was determined for the pluviometric stations, using long-term rainfall data sets obtained along with the Brazilian National Water Agency? ANA, which varied from 15 to 40 years. The monthly and annual erosivity indexes were generated using Fournier equation for Lavras, MG and the spatial distribution of rainfall erosivity was studied on the basis of geostatistical approaches considering only the distance which separates them, developing the isotropic experimental semivariogram. The semivariogram adjustment was done based on the Weighted Least Squares method and the spatial dependence degree. Once the structure and the semivariogram adjustment were defined, the ordinary kriging maps were created, providing erosivity spatial behavior in Southern Minas Gerais. It was observed that the Southern Minas Gerais presents high erosivity patterns, ranging from 5,145 to 7,776 MJ mm ha-1 h-1 year-1, in Ijaci (north of region) and Itajubá (southern region), respectively. Besides, it was verified that the erosivity indexes are intensely influenced by the topography, associated with climatic conditions. Higher erosivity is connected to areas with a higher altitude, such as along the Mantiqueira Range Mountain, and on high plateaus and mountain ranges in the North-Central part of the region. The geostatistical approach using longterm rainfall data in Southern region of Minas Gerais state, which is a relatively heterogeneous region in terms of altitude, soil depth and slope, showed to be adequate to the proposal of this study. MenosRainfall erosivity and its spatial variability were studied for 54 pluviometric stations in Southern Minas Gerais State (48º00? - 44º00?W; 23º50? - 20º00?S), aiming to plan the land-use strategies. Therefore, erosivity factor was determined for the pluviometric stations, using long-term rainfall data sets obtained along with the Brazilian National Water Agency? ANA, which varied from 15 to 40 years. The monthly and annual erosivity indexes were generated using Fournier equation for Lavras, MG and the spatial distribution of rainfall erosivity was studied on the basis of geostatistical approaches considering only the distance which separates them, developing the isotropic experimental semivariogram. The semivariogram adjustment was done based on the Weighted Least Squares method and the spatial dependence degree. Once the structure and the semivariogram adjustment were defined, the ordinary kriging maps were created, providing erosivity spatial behavior in Southern Minas Gerais. It was observed that the Southern Minas Gerais presents high erosivity patterns, ranging from 5,145 to 7,776 MJ mm ha-1 h-1 year-1, in Ijaci (north of region) and Itajubá (southern region), respectively. Besides, it was verified that the erosivity indexes are intensely influenced by the topography, associated with climatic conditions. Higher erosivity is connected to areas with a higher altitude, such as along the Mantiqueira Range Mountain, and on high plateaus and mountain ranges in the North-Centra... Mostrar Tudo |
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Palavras-Chave: |
Krigagem; Minas Gerais; Potencial erosivo; Sustentabilidade do solo. |
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Thesagro: |
Precipitação pluvial; Solo. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/938467/1/aquino.pdf
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Marc: |
LEADER 02503naa a2200253 a 4500
001 1938467
005 2012-11-09
008 2012 bl uuuu u00u1 u #d
100 1 $aAQUINO, R. F.
245 $aSpatial variability of the rainfall erosivity in southern region of Minas Gerais State, Brazil.
260 $c2012
520 $aRainfall erosivity and its spatial variability were studied for 54 pluviometric stations in Southern Minas Gerais State (48º00? - 44º00?W; 23º50? - 20º00?S), aiming to plan the land-use strategies. Therefore, erosivity factor was determined for the pluviometric stations, using long-term rainfall data sets obtained along with the Brazilian National Water Agency? ANA, which varied from 15 to 40 years. The monthly and annual erosivity indexes were generated using Fournier equation for Lavras, MG and the spatial distribution of rainfall erosivity was studied on the basis of geostatistical approaches considering only the distance which separates them, developing the isotropic experimental semivariogram. The semivariogram adjustment was done based on the Weighted Least Squares method and the spatial dependence degree. Once the structure and the semivariogram adjustment were defined, the ordinary kriging maps were created, providing erosivity spatial behavior in Southern Minas Gerais. It was observed that the Southern Minas Gerais presents high erosivity patterns, ranging from 5,145 to 7,776 MJ mm ha-1 h-1 year-1, in Ijaci (north of region) and Itajubá (southern region), respectively. Besides, it was verified that the erosivity indexes are intensely influenced by the topography, associated with climatic conditions. Higher erosivity is connected to areas with a higher altitude, such as along the Mantiqueira Range Mountain, and on high plateaus and mountain ranges in the North-Central part of the region. The geostatistical approach using longterm rainfall data in Southern region of Minas Gerais state, which is a relatively heterogeneous region in terms of altitude, soil depth and slope, showed to be adequate to the proposal of this study.
650 $aPrecipitação pluvial
650 $aSolo
653 $aKrigagem
653 $aMinas Gerais
653 $aPotencial erosivo
653 $aSustentabilidade do solo
700 1 $aSILVA, M. L. N.
700 1 $aFREITAS, D. A. F. de
700 1 $aCURI, N.
700 1 $aMELLO, C. R. de
700 1 $aAVANZI, J. C.
773 $tCiência e Agrotecnologia, Lavras$gv. 36, n. 5, p. 533-542, set./out. 2012.
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Embrapa Pesca e Aquicultura (CNPASA) |
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