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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
10/11/2017 |
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Data da última atualização: |
13/11/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GALVÃO, C. E.; FRAGOSO, S. P.; OLIVEIRA, C. E. de; FORNER, O.; PEREIRA, R. R. B.; SOARES, C. O.; ROSINHA, G. M. S. |
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Afiliação: |
CLEBER EDUARDO GALVÃO, Federal University of Mato Grosso do Sul (UFMS), Campo Grande, MS.; STENIO PERDIGÃO FRAGOSO, Carlos Chagas Institute, Oswaldo Cruz Foundation (ICC-FIOCRUZ), Curitiba, PR.; CARINA ELISEI DE OLIVEIRA, Dom Bosco Catholic University (UCDB), Campo Grande, MS.; ODINÉIA FORNER, Federal University of Paraná (UFPR), Curitiba, PR.; RENATA RIBEIRO BASTOS PEREIRA, Federal University of Mato Grosso do Sul (UFMS), Campo Grande, MS.; CLEBER OLIVEIRA SOARES, DE/TT; GRACIA MARIA SOARES ROSINHA, CNPGC. |
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Título: |
Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
BMC Microbiology, v. 17, n. 202, p. 1-8, sept., 2017. |
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DOI: |
10.1186/s12866-017-1110-7 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Caseous lymphadenitis (CLA) is a disease that affects sheep, goats and occasionally humans. The etiologic agent is the Corynebacterium pseudotuberculosis bacillus. The objective of this study was to build a gene expression library from C. pseudotuberculosis and use immunoscreening to identify genes that encode potential antigenic proteins for the development of DNA and subunit vaccines against CLA. Results: A wild strain of C. pseudotuberculosis was used for extraction and partial digestion of genomic DNA. Sequences between 1000 and 5000 base pairs (bp) were excised from the gel, purified, and the digested DNA fragments were joined to bacteriophage vector ZAP Express, packaged into phage and transfected into Escherichia coli . For immunoscreening a positive sheep sera pool and a negative sera pool for CLA were used. Four clones were identified that strongly reacted to sera. The clones were confirmed by polymerase chain reaction (PCR) followed by sequencing for genomic comparison of C. pseudotuberculosis in GenBank. The genes identified were dak2, fagA, fagB, NlpC/P60 protein family and LPxTG putative protein family. Conclusion: Proteins of this type can be antigenic which could aid in the development of subunit or DNA vaccines against CLA as well as in the development of serological tests for diagnosis. Immunoscreening of the gene expression library was shown to be a sensitive and efficient technique to identify probable immunodominant genes. |
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Palavras-Chave: |
DNA vaccines. |
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Thesagro: |
Corynebacterium Pseudotuberculosis. |
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Thesaurus Nal: |
Caseous lymphadenitis; Gene expression; Sheep. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/166571/1/Identification-of-new-Corynebacterium.pdf
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Marc: |
LEADER 02301naa a2200265 a 4500
001 2079448
005 2017-11-13
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1186/s12866-017-1110-7$2DOI
100 1 $aGALVÃO, C. E.
245 $aIdentification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library.$h[electronic resource]
260 $c2017
520 $aBackground: Caseous lymphadenitis (CLA) is a disease that affects sheep, goats and occasionally humans. The etiologic agent is the Corynebacterium pseudotuberculosis bacillus. The objective of this study was to build a gene expression library from C. pseudotuberculosis and use immunoscreening to identify genes that encode potential antigenic proteins for the development of DNA and subunit vaccines against CLA. Results: A wild strain of C. pseudotuberculosis was used for extraction and partial digestion of genomic DNA. Sequences between 1000 and 5000 base pairs (bp) were excised from the gel, purified, and the digested DNA fragments were joined to bacteriophage vector ZAP Express, packaged into phage and transfected into Escherichia coli . For immunoscreening a positive sheep sera pool and a negative sera pool for CLA were used. Four clones were identified that strongly reacted to sera. The clones were confirmed by polymerase chain reaction (PCR) followed by sequencing for genomic comparison of C. pseudotuberculosis in GenBank. The genes identified were dak2, fagA, fagB, NlpC/P60 protein family and LPxTG putative protein family. Conclusion: Proteins of this type can be antigenic which could aid in the development of subunit or DNA vaccines against CLA as well as in the development of serological tests for diagnosis. Immunoscreening of the gene expression library was shown to be a sensitive and efficient technique to identify probable immunodominant genes.
650 $aCaseous lymphadenitis
650 $aGene expression
650 $aSheep
650 $aCorynebacterium Pseudotuberculosis
653 $aDNA vaccines
700 1 $aFRAGOSO, S. P.
700 1 $aOLIVEIRA, C. E. de
700 1 $aFORNER, O.
700 1 $aPEREIRA, R. R. B.
700 1 $aSOARES, C. O.
700 1 $aROSINHA, G. M. S.
773 $tBMC Microbiology$gv. 17, n. 202, p. 1-8, sept., 2017.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
25/11/2021 |
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Data da última atualização: |
25/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
PAIXÃO, J. A. da; ARAÚJO NETO, J. F. de; NASCIMENTO, B. O. do; COSTA, D. M. da; BRANDÃO, H. N.; SOUZA, F. V. D.; SOUZA, E. H. de; ALVES, Q. L.; ERLING, S. B. L.; DAVID, J. P. de L. |
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Afiliação: |
JULIANA AZEVEDO DA PAIXÃO, UFBA; JOSÉ FERNANDO DE ARAÚJO NETO, UFBA; BRUNA OLIVEIRA DO NASCIMENTO, UFBA; DIEGO MOTA DA COSTA, UEFS; HUGO NEVES BRANDÃO, UEFS; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; EVERTON HILO DE SOUZA, UFRB; QUIARA LOVATTI ALVES, FIOCRUZ; SAMILE BARRETO LIMA ERLING, UNIJORGE; JUCENI PEREIRA DE LIMA DAVID, UFBA. |
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Título: |
Phharmacological actions of ananas comosus l. merril: revision of the works published from 1966 to 2020. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Pharmacognosy Reviews, v. 15, Issue 29, Jan./Jun., 2021. |
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ISSN: |
0973-7847 |
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Idioma: |
Inglês |
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Conteúdo: |
Bromeliaceae family is very common in Brazilian territory, with approximately 56 genera registered. Ananas comosus (L.) Merr, known as pineapple, one of the most consumed tropical fruits in the world, is the most studied species among them. Additionally some of the plants in this family are used to treat several pathologies in traditional medicine, so this study synthesize the main pharmacological or biological actions of A. comosus and its varieties. A literature review was conducted from the main indexed databases (PubMed, SciELO, Science Direct, Lilacs) about the biological activities of the species A. comosus and its varieties. The Ananas comosus var. comosus was the most cited in the research, we found different secondary metabolites, mainly flavonoids, phenolic compounds and also the complex of enzymes bromelain which were mainly responsible for the fifteen pharmacological activities found, such as abortive, antioxidant activity, hypolipidemic and hypoglycemic. The results obtained in this work show that the species presents several pharmacological applications that can be exploited as a source for new chemical and biological studies that lead to the isolation of promising substances |
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Thesagro: |
Abacaxi. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01970naa a2200253 a 4500
001 2136551
005 2021-11-25
008 2021 bl uuuu u00u1 u #d
022 $a0973-7847
100 1 $aPAIXÃO, J. A. da
245 $aPhharmacological actions of ananas comosus l. merril$brevision of the works published from 1966 to 2020.$h[electronic resource]
260 $c2021
520 $aBromeliaceae family is very common in Brazilian territory, with approximately 56 genera registered. Ananas comosus (L.) Merr, known as pineapple, one of the most consumed tropical fruits in the world, is the most studied species among them. Additionally some of the plants in this family are used to treat several pathologies in traditional medicine, so this study synthesize the main pharmacological or biological actions of A. comosus and its varieties. A literature review was conducted from the main indexed databases (PubMed, SciELO, Science Direct, Lilacs) about the biological activities of the species A. comosus and its varieties. The Ananas comosus var. comosus was the most cited in the research, we found different secondary metabolites, mainly flavonoids, phenolic compounds and also the complex of enzymes bromelain which were mainly responsible for the fifteen pharmacological activities found, such as abortive, antioxidant activity, hypolipidemic and hypoglycemic. The results obtained in this work show that the species presents several pharmacological applications that can be exploited as a source for new chemical and biological studies that lead to the isolation of promising substances
650 $aAbacaxi
700 1 $aARAÚJO NETO, J. F. de
700 1 $aNASCIMENTO, B. O. do
700 1 $aCOSTA, D. M. da
700 1 $aBRANDÃO, H. N.
700 1 $aSOUZA, F. V. D.
700 1 $aSOUZA, E. H. de
700 1 $aALVES, Q. L.
700 1 $aERLING, S. B. L.
700 1 $aDAVID, J. P. de L.
773 $tPharmacognosy Reviews$gv. 15, Issue 29, Jan./Jun., 2021.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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