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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
10/11/2017 |
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Data da última atualização: |
13/11/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GALVÃO, C. E.; FRAGOSO, S. P.; OLIVEIRA, C. E. de; FORNER, O.; PEREIRA, R. R. B.; SOARES, C. O.; ROSINHA, G. M. S. |
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Afiliação: |
CLEBER EDUARDO GALVÃO, Federal University of Mato Grosso do Sul (UFMS), Campo Grande, MS.; STENIO PERDIGÃO FRAGOSO, Carlos Chagas Institute, Oswaldo Cruz Foundation (ICC-FIOCRUZ), Curitiba, PR.; CARINA ELISEI DE OLIVEIRA, Dom Bosco Catholic University (UCDB), Campo Grande, MS.; ODINÉIA FORNER, Federal University of Paraná (UFPR), Curitiba, PR.; RENATA RIBEIRO BASTOS PEREIRA, Federal University of Mato Grosso do Sul (UFMS), Campo Grande, MS.; CLEBER OLIVEIRA SOARES, DE/TT; GRACIA MARIA SOARES ROSINHA, CNPGC. |
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Título: |
Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
BMC Microbiology, v. 17, n. 202, p. 1-8, sept., 2017. |
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DOI: |
10.1186/s12866-017-1110-7 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Caseous lymphadenitis (CLA) is a disease that affects sheep, goats and occasionally humans. The etiologic agent is the Corynebacterium pseudotuberculosis bacillus. The objective of this study was to build a gene expression library from C. pseudotuberculosis and use immunoscreening to identify genes that encode potential antigenic proteins for the development of DNA and subunit vaccines against CLA. Results: A wild strain of C. pseudotuberculosis was used for extraction and partial digestion of genomic DNA. Sequences between 1000 and 5000 base pairs (bp) were excised from the gel, purified, and the digested DNA fragments were joined to bacteriophage vector ZAP Express, packaged into phage and transfected into Escherichia coli . For immunoscreening a positive sheep sera pool and a negative sera pool for CLA were used. Four clones were identified that strongly reacted to sera. The clones were confirmed by polymerase chain reaction (PCR) followed by sequencing for genomic comparison of C. pseudotuberculosis in GenBank. The genes identified were dak2, fagA, fagB, NlpC/P60 protein family and LPxTG putative protein family. Conclusion: Proteins of this type can be antigenic which could aid in the development of subunit or DNA vaccines against CLA as well as in the development of serological tests for diagnosis. Immunoscreening of the gene expression library was shown to be a sensitive and efficient technique to identify probable immunodominant genes. |
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Palavras-Chave: |
DNA vaccines. |
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Thesagro: |
Corynebacterium Pseudotuberculosis. |
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Thesaurus Nal: |
Caseous lymphadenitis; Gene expression; Sheep. |
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Categoria do assunto: |
-- |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/1079448/1/IdentificationofnewCorynebacterium.pdf
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Marc: |
LEADER 02301naa a2200265 a 4500
001 2079448
005 2017-11-13
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1186/s12866-017-1110-7$2DOI
100 1 $aGALVÃO, C. E.
245 $aIdentification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library.$h[electronic resource]
260 $c2017
520 $aBackground: Caseous lymphadenitis (CLA) is a disease that affects sheep, goats and occasionally humans. The etiologic agent is the Corynebacterium pseudotuberculosis bacillus. The objective of this study was to build a gene expression library from C. pseudotuberculosis and use immunoscreening to identify genes that encode potential antigenic proteins for the development of DNA and subunit vaccines against CLA. Results: A wild strain of C. pseudotuberculosis was used for extraction and partial digestion of genomic DNA. Sequences between 1000 and 5000 base pairs (bp) were excised from the gel, purified, and the digested DNA fragments were joined to bacteriophage vector ZAP Express, packaged into phage and transfected into Escherichia coli . For immunoscreening a positive sheep sera pool and a negative sera pool for CLA were used. Four clones were identified that strongly reacted to sera. The clones were confirmed by polymerase chain reaction (PCR) followed by sequencing for genomic comparison of C. pseudotuberculosis in GenBank. The genes identified were dak2, fagA, fagB, NlpC/P60 protein family and LPxTG putative protein family. Conclusion: Proteins of this type can be antigenic which could aid in the development of subunit or DNA vaccines against CLA as well as in the development of serological tests for diagnosis. Immunoscreening of the gene expression library was shown to be a sensitive and efficient technique to identify probable immunodominant genes.
650 $aCaseous lymphadenitis
650 $aGene expression
650 $aSheep
650 $aCorynebacterium Pseudotuberculosis
653 $aDNA vaccines
700 1 $aFRAGOSO, S. P.
700 1 $aOLIVEIRA, C. E. de
700 1 $aFORNER, O.
700 1 $aPEREIRA, R. R. B.
700 1 $aSOARES, C. O.
700 1 $aROSINHA, G. M. S.
773 $tBMC Microbiology$gv. 17, n. 202, p. 1-8, sept., 2017.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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| Registros recuperados : 55 | |
| 9. |  | FERREIRA, R. R. M.; FERREIRA, V. M. Uso do modelo matemático SWAT para avaliar o impacto das práticas de manejo do solo. In: REUNIÃO BRASILEIRA DE MANEJO E CONSERVAÇÃO DO SOLO E DA ÁGUA, 20., 2016, Foz do Iguaçu. O Solo sob ameaça: conexões necessárias ao manejo e conservação do solo e água: anais. Curitiba: SBCS: IAPAR, 2016. p. 788-790. Editores: Arnaldo Colozzi Filho, João Henrique Caviglione, Graziela Moraes de Cesare Barbosa, Luciano Grillo Gil, Tiago Santos Telles.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Acre. |
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| 10. | | FERREIRA, R. R. M.; ANDRADE NETO, R. de C. Calagem e adubação. In: ANDRADE NETO, R. de C.; NOGUEIRA, S. R.; NASCIMENTO, G. C. do; NEGREIROS, J. R. da S.; GOMES, F. C. da R. Sistema de produção da cultura do abacaxi para o estado do Acre. Rio Branco, AC: Embrapa Acre, 2018. Publicação eletrônica. (Embrapa Acre. Sistema de produção, 9).| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Acre. |
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| 16. | | FERREIRA, R. R. M.; MIQUELONI, D. P.; SILVA, L. M. da; WADT, L. H. de O. Características químicas de solos florestais no Estado do Acre sob castanhal nativo. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 32.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 16.; Simpósio Brasileiro de Microbiologia do Solo, 14.; XI REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 11., 2016, Goiânia. Rumo aos novos desafios: anais. Goiânia: UFG: UFMT: Instituto Federal Goiano: Embrapa: UnB, 2016. 1 p. FertBio 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Acre; Embrapa Rondônia. |
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| Registros recuperados : 55 | |
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