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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
21/09/2017 |
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Data da última atualização: |
21/09/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BEARSON, B. L.; BEARSON, S. M. D.; BRUNELLE, B. W.; BAYLES, D. O.; LEE, I. S.; KICH, J. D. |
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Afiliação: |
BRADLEY L. BEARSON, USDA/ARS/National Laboratory for Agriculture and the Environment; SHAWN M. D. BEARSON, USDA/ARS/National Animal Disease Center; BRIAN W. BRUNELLE, USDA/ARS/National Animal Disease Center; DARRELL O. BAYLES, USDA/ARS/National Animal Disease Center; IN SOO LEE, Hannam University; JALUSA DEON KICH, CNPSA. |
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Título: |
Salmonella DIVA vaccine reduces disease, colonization and shedding due to virulent S. Typhimurium infection in swine. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Journal of Medical Microbiology, v. 66, p. 651-661, 2017. |
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DOI: |
10.1099/jmm.0.000482 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract Purpose. Non-host-adapted Salmonella serovars, including the common human food-borne pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium), are opportunistic pathogens that can colonize food-producing animals without causing overt disease. Interventions against Salmonella are needed to enhance food safety, protect animal health and allow the differentiation of infected from vaccinated animals (DIVA). Methodology. An attenuated S. Typhimurium DIVA vaccine (BBS 866) was characterized for the protection of pigs following challenge with virulent S. Typhimurium. The porcine transcriptional response to BBS 866 vaccination was evaluated. RNASeq analysis was used to compare gene expression between BBS 866 and its parent; phenotypic assays were performed to confirm transcriptional differences observed between the strains. Results. Vaccination significantly reduced fever and interferon-gamma (IFNg) levels in swine challenged with virulent S. Typhimurium compared to mock-vaccinated pigs. Salmonella faecal shedding and gastrointestinal tissue colonization were significantly lower in vaccinated swine. RNA-Seq analysis comparing BBS 866 to its parental S. Typhimurium strain demonstrated reduced expression of the genes involved in cellular invasion and bacterial motility; decreased invasion of porcinederived IPEC-J2 cells and swimming motility for the vaccine strain was consistent with the RNA-Seq analysis. Numerous membrane proteins were differentially expressed, which was an anticipated gene expression pattern due to the targeted deletion of several regulatory genes in the vaccine strain. RNA-Seq analysis indicated that genes involved in the porcine immune and inflammatory response were differentially regulated at 2 days post-vaccination compared to pre-vaccination. Conclusion. Evaluation of the S. Typhimurium DIVA vaccine indicates that vaccination will provide both swine health and food safety benefits. MenosAbstract Purpose. Non-host-adapted Salmonella serovars, including the common human food-borne pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium), are opportunistic pathogens that can colonize food-producing animals without causing overt disease. Interventions against Salmonella are needed to enhance food safety, protect animal health and allow the differentiation of infected from vaccinated animals (DIVA). Methodology. An attenuated S. Typhimurium DIVA vaccine (BBS 866) was characterized for the protection of pigs following challenge with virulent S. Typhimurium. The porcine transcriptional response to BBS 866 vaccination was evaluated. RNASeq analysis was used to compare gene expression between BBS 866 and its parent; phenotypic assays were performed to confirm transcriptional differences observed between the strains. Results. Vaccination significantly reduced fever and interferon-gamma (IFNg) levels in swine challenged with virulent S. Typhimurium compared to mock-vaccinated pigs. Salmonella faecal shedding and gastrointestinal tissue colonization were significantly lower in vaccinated swine. RNA-Seq analysis comparing BBS 866 to its parental S. Typhimurium strain demonstrated reduced expression of the genes involved in cellular invasion and bacterial motility; decreased invasion of porcinederived IPEC-J2 cells and swimming motility for the vaccine strain was consistent with the RNA-Seq analysis. Numerous membrane proteins were differentially expressed, whic... Mostrar Tudo |
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Thesagro: |
Salmonella Typhimurium; Suinocultura; Vacina; Virologia. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02678naa a2200241 a 4500
001 2076069
005 2017-09-21
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1099/jmm.0.000482$2DOI
100 1 $aBEARSON, B. L.
245 $aSalmonella DIVA vaccine reduces disease, colonization and shedding due to virulent S. Typhimurium infection in swine.$h[electronic resource]
260 $c2017
520 $aAbstract Purpose. Non-host-adapted Salmonella serovars, including the common human food-borne pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium), are opportunistic pathogens that can colonize food-producing animals without causing overt disease. Interventions against Salmonella are needed to enhance food safety, protect animal health and allow the differentiation of infected from vaccinated animals (DIVA). Methodology. An attenuated S. Typhimurium DIVA vaccine (BBS 866) was characterized for the protection of pigs following challenge with virulent S. Typhimurium. The porcine transcriptional response to BBS 866 vaccination was evaluated. RNASeq analysis was used to compare gene expression between BBS 866 and its parent; phenotypic assays were performed to confirm transcriptional differences observed between the strains. Results. Vaccination significantly reduced fever and interferon-gamma (IFNg) levels in swine challenged with virulent S. Typhimurium compared to mock-vaccinated pigs. Salmonella faecal shedding and gastrointestinal tissue colonization were significantly lower in vaccinated swine. RNA-Seq analysis comparing BBS 866 to its parental S. Typhimurium strain demonstrated reduced expression of the genes involved in cellular invasion and bacterial motility; decreased invasion of porcinederived IPEC-J2 cells and swimming motility for the vaccine strain was consistent with the RNA-Seq analysis. Numerous membrane proteins were differentially expressed, which was an anticipated gene expression pattern due to the targeted deletion of several regulatory genes in the vaccine strain. RNA-Seq analysis indicated that genes involved in the porcine immune and inflammatory response were differentially regulated at 2 days post-vaccination compared to pre-vaccination. Conclusion. Evaluation of the S. Typhimurium DIVA vaccine indicates that vaccination will provide both swine health and food safety benefits.
650 $aSalmonella Typhimurium
650 $aSuinocultura
650 $aVacina
650 $aVirologia
700 1 $aBEARSON, S. M. D.
700 1 $aBRUNELLE, B. W.
700 1 $aBAYLES, D. O.
700 1 $aLEE, I. S.
700 1 $aKICH, J. D.
773 $tJournal of Medical Microbiology$gv. 66, p. 651-661, 2017.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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| Registros recuperados : 34 | |
| 5. |  | PEREIRA, M. C.; JABLONSKI, A.; RIOS, A. de O.; VIZZOTTO, M.; FLÔRES, S. H. Perfil de carotenoides e teor de vitamina c em frutos Brasileiros. In: SIMPÓSIO INTERNACIONAL DE PLANTAS MEDICINAIS E NUTRACÊUTICOS, 3.; CONFERÊNCIA DO INSTITUTO NACIONAL DE CIÊNCIA E TECNOLOGIA DE FRUTOS TROPICAIS, 3., 2012, Aracajú. Anais... Aracajú: UFS, 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 7. | | CARVALHO, A. V.; RIOS, A. de O.; MACIEL, R. A.; MORESCO, K. S.; BECKMAN, J. C. Determinação de carotenoides e atividade antioxidante de pimentas provenientes da região amazônica. Magistra, v. 25, n. esp., 2013. Anais do 3º Congresso Brasileiro de Processamento de Frutas e Hortaliças, 2013, Ilhéus.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 8. | | PEREIRA, M. C.; OLIVEIRA, A.; RIOS, A. de O.; VIZZOTTO, M.; FLORES, S. H. Teor de compostos fenólicos totais e potencial antioxidante de geléias e schimier de pequenas frutas da região de clima temperado. In: SIMPÓSIO NACIONAL DO MORANGO, 5.; ENCONTRO SOBRE PEQUENAS FRUTAS E FRUTAS NATIVAS DO MERCOSUL, 4., 2010, Pelotas. Palestras e resumos... Pelotas: Embrapa Clima Temperado, 2010. p. 201-202| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 11. | | PEREIRA, M. C.; STEFFENS, R. S.; RIOS, A. de O.; HERTZ, P. F.; VIZZOTTO, M.; FLORES, S. H. Caracterização físico-química e capacidade antioxidante dos frutos do araçazeiro coletados no estado do Rio Grande do Sul. In: SIMPÓSIO DE SEGURANÇA ALIMENTAR, 3., 2010, Florianópolis. Rompendo Barreiras: anais... Florianópolis: sbCTA-RS, 2010.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 12. | | PEREIRA, M. C.; STEFFENS, R. S.; RIOS, A. de O.; HERTZ, P. F.; VIZZOTTO, M.; FLORES, S. H. Caracterização físico-química e capacidade antioxidante dos frutos da guabirobeira coletados no estado do Rio Grande do Sul. In: SIMPÓSIO DE SEGURANÇA ALIMENTAR, 3., 2010, Florianópolis. Rompendo Barreiras: anais... Florianópolis: sbCTA-RS, 2010.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 13. | | CARVALHO, A. V.; MATTIETTO, R. de A.; RIOS, A. de O.; FIGUEIREDO NETO, G. A.; BASSINELLO, P. Z. Análise sensorial de snack extrudado a partir de farinha de arroz e feijão. In: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 22., 2010, Salvador. Ciência e tecnologia de alimentos: potencialidades, desafios e inovações. [S.l.]: SBCTA, 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Arroz e Feijão. |
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| 15. | | BASSINELLO, P. Z.; CARVALHO, A. V.; RIOS, A. de O.; MACIEL, R. de A.; BERRIOS, J. de J. Expanded gluten-free extrudates made from rice grits and bandinha (bean) flour mixes: main quality properties. Journal of Food Processing and Preservation, Westport, v. 39, n. 6, p. 2267-2275, Dec. 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Arroz e Feijão. |
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| 16. | | PEREIRA, M. C.; STEFFENS, R. S.; JABLONSKI, A.; HERTZ, P. F.; RIOS, A. de O.; VIZZOTTO, M.; FLORES, S. H. Characterization and Antioxidant Potential of Brazilian Fruits from the Myrtaceae Family. Journal of Agricultural and Food Chemistry, v. 60, p. 3061-3067, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 17. | | PEREIRA, M. C.; STEFFENS, R. S.; JABLONSKI, A.; HERTZ, P. F.; RIOS, A. de O.; VIZZOTTO, M.; FLORES, S. H. Characterization, bioactive compounds and antioxidant potential of three Brazilian fruits. Journal of Food Composition and Analysis, V. 29, P. 19?24, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 18. | | CARVALHO, A. V.; BASSINELLO, P. Z.; RIOS, A. de O.; FERREIRA, T. F.; CARVALHO, R. N.; KOAKUZU, S. N. Characterization of pre-gelatinized rice and bean flour. Food Science and Technology, v. 33, n. 2, p. 245-250, Apr./June, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Arroz e Feijão. |
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| 19. | | CARVALHO, A. V.; BASSINELLO, P. Z.; MATTIETTO, R. de A.; CARVALHO, R. N.; RIOS, A. de O.; SECCADIO, L. L. Processamento e caracterização de snack extrudado a partir de farinhas de quirera de arroz e de bandinha de feijão. Brazilian Journal of Food Technology, Campinas, v. 15, n. 1, p. 72-83, jan./mar. 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Arroz e Feijão. |
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| 20. | | TORMA, P. C. M. R.; TUPUNA, S. D.; LIMA, P.; CARVALHO, A. V.; FLÔRES, S. H.; AUGUSTI, P. R.; RIOS, A. de O. Identificação e quantificação de carotenoides em novos acessos de açaí. In: CONGRESSO SUL BRASILEIRO DE ENGENHARIA DE ALIMENTOS, 2., 2015, Blumenau. Anais. Blumenau: FURB, 2015. Resumo e pôster.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| Registros recuperados : 34 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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