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Registro Completo
Biblioteca(s):  Embrapa Caprinos e Ovinos.
Data corrente:  16/06/2017
Data da última atualização:  16/06/2017
Autoria:  TSONIS, C. G.; McNEILLY, A. S.; BAIRD, D. T.
Título:  Measurement of exogenous and endogenous inhibin in sheep serum using a new and extremely sensitive bioassay for inhibin based on inhibition of ovine pituitary FSH secretion in vitro.
Ano de publicação:  1986
Fonte/Imprenta:  Journal of Endocrinology, v. 110, n. 2, p. 341-352, Aug. 1986.
DOI:  10.1677/joe.0.1100341
Idioma:  Inglês
Conteúdo:  Abstract: An extremely sensitive and reliable bioassay for inhibin based on inhibition of ovine pituitary FSH secretion in vitro was developed and used to measure exogenous and endogenous inhibin activity in the ewe. The sheep inhibin bioassay is 30- to 40-fold more sensitive than conventional rat inhibin bioassays. The minimum sensitivity of each bioassay in the measurement of inhibin activity in 1 ml of sheep serum is 220 mu. and 4080 mu. in the sheep and rat bioassays respectively. This sensitive inhibin bioassay has permitted, for the first time, the measurement of endogenous inhibin in the peripheral and ovarian vein blood of the sheep, as well as exogenously administered inhibin. The half-life of exogenously administered ovine inhibin (in follicular fluid) in the sheep was calculated as two components (18-24 and 50-60 min) from the inhibin profiles of six ewes. Inhibin contained in the ovine follicular fluid, given as a bolus i.v. injection, increased to maximum levels after 5 min and then remained increased for 10-32 min depending upon the dose administered, before exponentially decaying. The time for inhibin to exert its effect ranged from 3 to 6 h after injection and appeared to be dose-related. The bolus injection of inhibin, apart from causing suppression of FSH, evoked a large rebound increase of FSH up to 400% of preinjection levels. The development of the sheep bioassay will allow the measurement of biologically active inhibin in the peripheral circulation and ... Mostrar Tudo
Palavras-Chave:  Biological Assay; Blood supply; Drug effects; FSH; Half-Life; Inhibins; Ovaries.
Thesaurus NAL:  Pharmacology; pituitary gland; Rats; Sheep.
Categoria do assunto:  L Ciência Animal e Produtos de Origem Animal
Marc:  Mostrar Marc Completo
Registro original:  Embrapa Caprinos e Ovinos (CNPC)
Biblioteca ID Origem Tipo/Formato Classificação Cutter Registro Volume Status
CNPC33838 - 1ADDAP - PP

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