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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
14/02/2017 |
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Data da última atualização: |
30/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
NANI, T. F.; PEREIRA, D. L.; SOUZA SOBRINHO, F. de; TECHIO, V. H. |
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Afiliação: |
Thaís Furtado Nani, UFLA; Daniele Laís Pereira, UFLA; FAUSTO DE SOUZA SOBRINHO, CNPGL; Vânia Helena Techio, UFLA. |
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Título: |
Physical map of repetitive DNA sites in Brachiaria spp.: intravarietal and interspecific polymorphisms. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Crop Science, v. 56, n. 4, p. 1769-1783, 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract Brachiaria (Trin.) Griseb. is a genus belonging to the Poaceae Barnh. family that aggregates species of forage interest, which have shown different levels of ploidy, intraspecific variability for chromosomal morphology and for number of rDNA sites. This study aimed at characterizing the karyotype of Brachiaria brizantha (Hochst. ex A. Rich) Stapf, Brachiaria decumbens Stapf, and Brachiaria ruziziensis R. Germ. & C.M. Evrard by the location of 5S rDNA, 45S rDNA, centromeric retrotransposons, chromomycin A3 (CMA) and 4¢, 6-diamidino-2-phenylindole dihydrochloride (DAPI) chromosome bands, and transcriptional activity at nucleolar organizing regions (NORs). Brachiaria ruziziensis has 2n = 2x = 18 chromosomes, karyotypic formula 9M, one 45S rDNA site, and two 5S rDNA sites. Brachiaria brizantha has 2n = 4x = 36 chromosomes, karyotypic formula 18M, four to six 5S rDNA signals, and two to four 45S rDNA sites. One of the chromosomes carrying 45S rDNA is heteromorphic. Brachiaria decumbens has 2n = 4x = 36 chromosomes, karyotypic formula 17M+1M/SM, and seven 5S rDNA signals including one hemizygous 5S rDNA locus. One of the two chromosome pairs carrying 45S rDNA signals was heteromorphic with one of its chromosomes possessing a tertiary constriction. Hybridization signals with a centromeric retrotransposon probe, CRM1, were observed in Brachiaria chromosomes with distinct clusters on all B. ruziziensis chromosomes. Chromosomal CMA bands were located along with 45S rDNA signals in B. decumbens and B. ruziziensis whereas in B. brizantha only two out of four signals coincided. The karyotype of Brachiaria species is symmetrical. The transcriptional activity of NORs is variable with two or three heteromorphic nucleoli. MenosAbstract Brachiaria (Trin.) Griseb. is a genus belonging to the Poaceae Barnh. family that aggregates species of forage interest, which have shown different levels of ploidy, intraspecific variability for chromosomal morphology and for number of rDNA sites. This study aimed at characterizing the karyotype of Brachiaria brizantha (Hochst. ex A. Rich) Stapf, Brachiaria decumbens Stapf, and Brachiaria ruziziensis R. Germ. & C.M. Evrard by the location of 5S rDNA, 45S rDNA, centromeric retrotransposons, chromomycin A3 (CMA) and 4¢, 6-diamidino-2-phenylindole dihydrochloride (DAPI) chromosome bands, and transcriptional activity at nucleolar organizing regions (NORs). Brachiaria ruziziensis has 2n = 2x = 18 chromosomes, karyotypic formula 9M, one 45S rDNA site, and two 5S rDNA sites. Brachiaria brizantha has 2n = 4x = 36 chromosomes, karyotypic formula 18M, four to six 5S rDNA signals, and two to four 45S rDNA sites. One of the chromosomes carrying 45S rDNA is heteromorphic. Brachiaria decumbens has 2n = 4x = 36 chromosomes, karyotypic formula 17M+1M/SM, and seven 5S rDNA signals including one hemizygous 5S rDNA locus. One of the two chromosome pairs carrying 45S rDNA signals was heteromorphic with one of its chromosomes possessing a tertiary constriction. Hybridization signals with a centromeric retrotransposon probe, CRM1, were observed in Brachiaria chromosomes with distinct clusters on all B. ruziziensis chromosomes. Chromosomal CMA bands were located along with 45S rDNA signa... Mostrar Tudo |
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Palavras-Chave: |
CRM1; Cytogenetic analyses; Intravarietal variations; Karyotypes of Brachiaria spp. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02381naa a2200205 a 4500
001 2063953
005 2023-01-30
008 2016 bl uuuu u00u1 u #d
100 1 $aNANI, T. F.
245 $aPhysical map of repetitive DNA sites in Brachiaria spp.$bintravarietal and interspecific polymorphisms.$h[electronic resource]
260 $c2016
520 $aAbstract Brachiaria (Trin.) Griseb. is a genus belonging to the Poaceae Barnh. family that aggregates species of forage interest, which have shown different levels of ploidy, intraspecific variability for chromosomal morphology and for number of rDNA sites. This study aimed at characterizing the karyotype of Brachiaria brizantha (Hochst. ex A. Rich) Stapf, Brachiaria decumbens Stapf, and Brachiaria ruziziensis R. Germ. & C.M. Evrard by the location of 5S rDNA, 45S rDNA, centromeric retrotransposons, chromomycin A3 (CMA) and 4¢, 6-diamidino-2-phenylindole dihydrochloride (DAPI) chromosome bands, and transcriptional activity at nucleolar organizing regions (NORs). Brachiaria ruziziensis has 2n = 2x = 18 chromosomes, karyotypic formula 9M, one 45S rDNA site, and two 5S rDNA sites. Brachiaria brizantha has 2n = 4x = 36 chromosomes, karyotypic formula 18M, four to six 5S rDNA signals, and two to four 45S rDNA sites. One of the chromosomes carrying 45S rDNA is heteromorphic. Brachiaria decumbens has 2n = 4x = 36 chromosomes, karyotypic formula 17M+1M/SM, and seven 5S rDNA signals including one hemizygous 5S rDNA locus. One of the two chromosome pairs carrying 45S rDNA signals was heteromorphic with one of its chromosomes possessing a tertiary constriction. Hybridization signals with a centromeric retrotransposon probe, CRM1, were observed in Brachiaria chromosomes with distinct clusters on all B. ruziziensis chromosomes. Chromosomal CMA bands were located along with 45S rDNA signals in B. decumbens and B. ruziziensis whereas in B. brizantha only two out of four signals coincided. The karyotype of Brachiaria species is symmetrical. The transcriptional activity of NORs is variable with two or three heteromorphic nucleoli.
653 $aCRM1
653 $aCytogenetic analyses
653 $aIntravarietal variations
653 $aKaryotypes of Brachiaria spp
700 1 $aPEREIRA, D. L.
700 1 $aSOUZA SOBRINHO, F. de
700 1 $aTECHIO, V. H.
773 $tCrop Science$gv. 56, n. 4, p. 1769-1783, 2016.
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