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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
10/02/2017 |
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Data da última atualização: |
30/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SALIMENA, A. P. S.; LANGE, C. C.; CAMUSSONE, C.; SIGNORINI, M.; CALVINHO, L. F.; BRITO, M. A. V. P. e; BORGES, C. A. V.; GUIMARAES, A. S.; RIBEIRO, J. B.; MENDONCA, L. C.; PICCOLI, R. H. |
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Afiliação: |
Alessandra P. S. Salimena, UFLA; CARLA CHRISTINE LANGE, CNPGL; Cecilia Camussone, INTA; Marcelo Signorini, INTA; Luis F. Calvinho, INTA; MARIA APARECIDA V PAIVA E BRITO, CNPGL; CRISTIANO AMANCIO VIEIRA BORGES, CNPGL; ALESSANDRO DE SA GUIMARAES, CNPGL; JOAO BATISTA RIBEIRO, CNPGL; LETICIA CALDAS MENDONCA, CNPGL; Roberta H. Piccoli, UFLA. |
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Título: |
Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Veterinary Research Communications, v. 40, n. 3, p. 97-106, 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract Staphylococcus aureus is a pathogen that frequently causes mastitis in bovine herds worldwide. This pathogen produces several virulence factors, including cell-associated adhesins, toxic and cytolytic exoproteins, and capsular polysaccharides. The aim of the present study was to test for the presence of genes involved in capsular polysaccharide production and biofilm formation in S. aureus isolated from bovine mastitis samples collected from 119 dairy herds located in three different Brazilian regions, as well as to assay the production of capsular polysaccharides and biofilm, in vitro. The detection of the cap, icaAD, and bap genes was performed using PCR. The detection and quantification of capsular polysaccharide production was performed using ELISA assays. The ability of the isolates to form a biofilm was examined using the polystyrene surface of microtiter plates. All 159 S. aureus isolates investigated harboured the cap gene: 80 % carried the cap5 gene and 20 % carried the cap8 gene. Sixty-nine percent of the isolates expressed capsular polysaccharide (CP) in vitro, 58 % expressed CP5 and 11 % expressed CP8. All of the isolates harboured the icaA and icaD genes, and 95.6 % of the isolates carried the bap gene. Of the 159 isolates analysed, 97.5 % were biofilm producers. A significant association between the capsular genotype and phenotype and the amount of biofilm formation was detected: cap5/CP5 isolates tended to form more biofilm and to produce a thinner CP layer than cap8/CP8 isolates. The results indicate a high potential for pathogenicity among S. aureus isolated from bovine milk collected from three different regions in Brazil. MenosAbstract Staphylococcus aureus is a pathogen that frequently causes mastitis in bovine herds worldwide. This pathogen produces several virulence factors, including cell-associated adhesins, toxic and cytolytic exoproteins, and capsular polysaccharides. The aim of the present study was to test for the presence of genes involved in capsular polysaccharide production and biofilm formation in S. aureus isolated from bovine mastitis samples collected from 119 dairy herds located in three different Brazilian regions, as well as to assay the production of capsular polysaccharides and biofilm, in vitro. The detection of the cap, icaAD, and bap genes was performed using PCR. The detection and quantification of capsular polysaccharide production was performed using ELISA assays. The ability of the isolates to form a biofilm was examined using the polystyrene surface of microtiter plates. All 159 S. aureus isolates investigated harboured the cap gene: 80 % carried the cap5 gene and 20 % carried the cap8 gene. Sixty-nine percent of the isolates expressed capsular polysaccharide (CP) in vitro, 58 % expressed CP5 and 11 % expressed CP8. All of the isolates harboured the icaA and icaD genes, and 95.6 % of the isolates carried the bap gene. Of the 159 isolates analysed, 97.5 % were biofilm producers. A significant association between the capsular genotype and phenotype and the amount of biofilm formation was detected: cap5/CP5 isolates tended to form more biofilm and to produce a thinner CP... Mostrar Tudo |
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Palavras-Chave: |
Adhesin; Bap; Cap; Capsule; IcaAD. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 3 | |
| 1. |  | AMORA, D. X.; BRESSO, E.; TOGAWA, R. C.; GRYNBERG, P.; MAIGRET, B.; MARTINS, N. F. Prediction of G protein coupled receptors from plant genomes. International Journal of Current Research in Biosciences and Plant Biology, v. 3, n. 11, p. 92-107, 2016. (Online).| Tipo: Artigo em Periódico Indexado | Circulação/Nível: C - 0 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 2. | | BRESSO, E.; FERNANDEZ, D.; AMORA, D. X.; NOEL, P.; PETITOT, A.-S.; SA, M. E. L. de; ALBUQUERQUE, E. V. S.; DANCHIN, E. G. J.; MAIGRET, B.; MARTINS, N. F. A chemosensory GPCR as a potential target to control the Root-Knot Nematode Meloidogyne incognita parasitism in plants. Molecules, v. 24, n. 20, article 3798, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 3. | | MENDES, R. A. G.; BASSO, M. F.; AMORA, D. X.; SILVA, A. P.; PAES-DE-MELO, B.; TOGAWA, R. C.; FREIRE, E. V. S. A.; LISEI-DE-SA, M. E.; MACEDO, L. L. P. de; LOURENCO, I. T.; SA, M. F. G. de. In planta RNAi approach targeting three M. incognita effector genes disturbed the process of infection and reduced plant susceptibility. Experimental Parasitology, v. 238, 2022, 108246. Na publicação: Erika Valéria Saliba Albuquerque; Leonardo Lima Pepino Macedo; Isabela Tristan Lourenço-Tessutti; Maria Fatima Grossi-de-Sa.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 3 | |
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