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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
17/01/2017 |
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Data da última atualização: |
02/03/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BAPTISTA, R. C.; FERNANDES, M. A. M.; GILAVERTE, S.; QUEIROZ, S. C. do N. de; ASSALIN, M. R.; FERRACINI, V. L.; MONTEIRO, A. L. G.; REYES, F. G. R. |
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Afiliação: |
RAFAELA DE CARVALHO BAPTISTA, FEA-UNICAMP; MARIA ANGELA MACHADO FERNANDES, LAPOC-UFPR; SUSANA GILAVERTE, LAPOC-UFPR; SONIA CLAUDIA DO N DE QUEIROZ, CNPMA; MARCIA REGINA ASSALIN, CNPMA; VERA LUCIA FERRACINI, CNPMA; ALDA LUCIA GOMES MONTEIRO, LAPOC-UFPR; FELIX GUILLERMO REYES REYES, FEA-UNICAMP. |
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Título: |
Determination of moxidectin in serum by liquid chromatography-tandem mass spectrometry and its application in pharmacokinetic study in lambs. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Journal of the Brazilian Chemical Society, Campinas, v. 28, n. 2, p. 250-256, 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The widespread use of moxidectin (MOX), a parasiticide used in the sheep breeding, has induced the parasite resistance in Brazilian farms. As a consequence, the farmers often increase the dose and frequency of drug utilization, and disregards safety of meat or milk. In order to establish adequate therapeutic treatment it is necessary to know the pharmacokinetics of the drug in the animal's body. Thus, high performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS) method was developed for the determination of MOX in serum lamb. Serum samples were treated with acetonitrile to precipitate proteins. A clean up by dispersive extraction in solid phase (SPE-d), using primary/secondary amine (PSA) and C18 sorbents, followed by freezing was performed. Method validation presented precision (coefficient of variation) and accuracy (recovery%) between 1.7-6.7 and 80.0-107.3%, respectively. The limit of quantification (LOQ) of the method was 2.0 ng mL-1 and a linear response was obtained over a range of 2.0 to 100 ng mL-1. This method was successfully applied to the determination of MOX in serum from suffolk lamb to evaluate the pharmacokinetic profile. |
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Palavras-Chave: |
LC-MS/MS; Serum lamb. |
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Thesagro: |
Análise de laboratório; Anti-helmíntico; Cordeiro; Cromatografia; Espectrometria; Soro Sangüíneo. |
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Thesaurus Nal: |
Anthelmintics; Blood serum; Lambs; Liquid chromatography; Moxidectin; Pharmacokinetics; Tandem mass spectrometry; Veterinary drugs. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/153417/1/2016AP13.pdf
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Marc: |
LEADER 02416naa a2200397 a 4500
001 2060930
005 2017-03-02
008 2017 bl uuuu u00u1 u #d
100 1 $aBAPTISTA, R. C.
245 $aDetermination of moxidectin in serum by liquid chromatography-tandem mass spectrometry and its application in pharmacokinetic study in lambs.$h[electronic resource]
260 $c2017
520 $aAbstract: The widespread use of moxidectin (MOX), a parasiticide used in the sheep breeding, has induced the parasite resistance in Brazilian farms. As a consequence, the farmers often increase the dose and frequency of drug utilization, and disregards safety of meat or milk. In order to establish adequate therapeutic treatment it is necessary to know the pharmacokinetics of the drug in the animal's body. Thus, high performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS) method was developed for the determination of MOX in serum lamb. Serum samples were treated with acetonitrile to precipitate proteins. A clean up by dispersive extraction in solid phase (SPE-d), using primary/secondary amine (PSA) and C18 sorbents, followed by freezing was performed. Method validation presented precision (coefficient of variation) and accuracy (recovery%) between 1.7-6.7 and 80.0-107.3%, respectively. The limit of quantification (LOQ) of the method was 2.0 ng mL-1 and a linear response was obtained over a range of 2.0 to 100 ng mL-1. This method was successfully applied to the determination of MOX in serum from suffolk lamb to evaluate the pharmacokinetic profile.
650 $aAnthelmintics
650 $aBlood serum
650 $aLambs
650 $aLiquid chromatography
650 $aMoxidectin
650 $aPharmacokinetics
650 $aTandem mass spectrometry
650 $aVeterinary drugs
650 $aAnálise de laboratório
650 $aAnti-helmíntico
650 $aCordeiro
650 $aCromatografia
650 $aEspectrometria
650 $aSoro Sangüíneo
653 $aLC-MS/MS
653 $aSerum lamb
700 1 $aFERNANDES, M. A. M.
700 1 $aGILAVERTE, S.
700 1 $aQUEIROZ, S. C. do N. de
700 1 $aASSALIN, M. R.
700 1 $aFERRACINI, V. L.
700 1 $aMONTEIRO, A. L. G.
700 1 $aREYES, F. G. R.
773 $tJournal of the Brazilian Chemical Society, Campinas$gv. 28, n. 2, p. 250-256, 2017.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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| Registros recuperados : 2 | |
| 1. |  | BAPTISTA, R. C.; FERNANDES, M. A. M.; GILAVERTE, S.; QUEIROZ, S. C. do N. de; ASSALIN, M. R.; FERRACINI, V. L.; MONTEIRO, A. L. G.; REYES, F. G. R. Determination of moxidectin in serum by liquid chromatography-tandem mass spectrometry and its application in pharmacokinetic study in lambs. Journal of the Brazilian Chemical Society, Campinas, v. 28, n. 2, p. 250-256, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 2. | | BAPTISTA, R. C.; FERNANDES, M. A. M.; GILAVERTE, S.; QUEIROZ, S. C. do N. de; FERRACINI, V. L.; ASSALIN, M. R.; MONTEIRO, A. L. G.; REYES, F. G. R. Pharmacokinetic parameters of moxidectin residue in the serum of lambs. In: ANNUAL MEETING BRAZILIAN SOCIETY OF ANIMAL SCIENCE, 50., 2013, Campinas. The integration of knowledge in animal production: abstracts. Campinas: SBZ, 2013. 1 CD ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| Registros recuperados : 2 | |
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