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Registro Completo |
Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
05/12/2016 |
Data da última atualização: |
05/12/2016 |
Autoria: |
FLÓREZ, L. M. M.; BALLESTERO, H. F.; DUZANSKI, A. P.; BERSANO, P. R. O.; LIMA, J. F.; CRUZ, F. L.; MOTA, L. S.; ROCHA, N. S. |
Afiliação: |
LUIS M. M. FLÓREZ, UNESP/Universidade de Caldas; HALINE F. BALLESTERO, UNESP; ANDERSON P. DUZANSKI, UNESP; PAULO R. O. BERSANO, UECE; JOÃO F. LIMA, CEVAP/UNESP; FERNANDA L. CRUZ, UNESP; LÍGIA S. MOTA, UNESP; NOEME S. ROCHA, UNESP. |
Título: |
Immunocytochemical characterization of primary cell culture in canine transmissible venereal tumor. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Brasília, DF, v. 39, n. 9, p. 844-850, set. 2016. |
Idioma: |
Inglês |
Conteúdo: |
Immunochemistry with anti-vimentin, anti-lysozyme, anti-alpha 1 antitrypsin, anti-CD3 and anti-CD79α antibodies has been used for characterization of primary cell culture in the transmissible venereal tumor (TVT). Samples for primary cell culture and immunohistochemistry assays were taken from eight dogs with cytological and clinical diagnosis of TVT. To validate the immunochemical results in the primary cell culture of TVT, a chromosome count was performed. For the statistical analysis, the Mann-Whitney test with p<0.05 was used. TVT tissues and culture cells showed intense anti-vimentin immunoreactivity, lightly to moderate immunoreactivity for anti-lysozyme, and mild for anti-alpha-antitrypsin. No marking was achieved for CD3 and CD79α. All culture cells showed chromosomes variable number of 56 to 68. This is the first report on the use of immunocytochemical characterization in cell culture of TVT. Significant statistic difference between immunochemistry in tissue and culture cell was not established, what suggests that the use of this technique may provide greater certainty for the confirmation of tumors in the primary culture. This fact is particularly important because in vitro culture of tumor tissues has been increasingly used to provide quick access to drug efficacy and presents relevant information to identify potential response to anticancer medicine; so it is possible to understand the behavior of the tumor. |
Palavras-Chave: |
Alfa 1 antitripsina; Alpha 1 antitrypsin; Canine transmissible venereal tumo; Isozima; Tumor venéreo transmissível canino; Vimentina. |
Thesagro: |
Citogenética. |
Thesaurus Nal: |
Cytogenetics; Lysozyme; Vimentin. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/151255/1/Immunocytochemical-characterization.pdf
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Marc: |
LEADER 02414naa a2200325 a 4500 001 2057977 005 2016-12-05 008 2016 bl uuuu u00u1 u #d 100 1 $aFLÓREZ, L. M. M. 245 $aImmunocytochemical characterization of primary cell culture in canine transmissible venereal tumor. 260 $c2016 520 $aImmunochemistry with anti-vimentin, anti-lysozyme, anti-alpha 1 antitrypsin, anti-CD3 and anti-CD79α antibodies has been used for characterization of primary cell culture in the transmissible venereal tumor (TVT). Samples for primary cell culture and immunohistochemistry assays were taken from eight dogs with cytological and clinical diagnosis of TVT. To validate the immunochemical results in the primary cell culture of TVT, a chromosome count was performed. For the statistical analysis, the Mann-Whitney test with p<0.05 was used. TVT tissues and culture cells showed intense anti-vimentin immunoreactivity, lightly to moderate immunoreactivity for anti-lysozyme, and mild for anti-alpha-antitrypsin. No marking was achieved for CD3 and CD79α. All culture cells showed chromosomes variable number of 56 to 68. This is the first report on the use of immunocytochemical characterization in cell culture of TVT. Significant statistic difference between immunochemistry in tissue and culture cell was not established, what suggests that the use of this technique may provide greater certainty for the confirmation of tumors in the primary culture. This fact is particularly important because in vitro culture of tumor tissues has been increasingly used to provide quick access to drug efficacy and presents relevant information to identify potential response to anticancer medicine; so it is possible to understand the behavior of the tumor. 650 $aCytogenetics 650 $aLysozyme 650 $aVimentin 650 $aCitogenética 653 $aAlfa 1 antitripsina 653 $aAlpha 1 antitrypsin 653 $aCanine transmissible venereal tumo 653 $aIsozima 653 $aTumor venéreo transmissível canino 653 $aVimentina 700 1 $aBALLESTERO, H. F. 700 1 $aDUZANSKI, A. P. 700 1 $aBERSANO, P. R. O. 700 1 $aLIMA, J. F. 700 1 $aCRUZ, F. L. 700 1 $aMOTA, L. S. 700 1 $aROCHA, N. S. 773 $tPesquisa Veterinária Brasileira, Brasília, DF$gv. 39, n. 9, p. 844-850, set. 2016.
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Embrapa Unidades Centrais (AI-SEDE) |
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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Suínos e Aves. |
Data corrente: |
25/11/2009 |
Data da última atualização: |
18/10/2010 |
Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
Autoria: |
LUDTKE, C. B.; DALLA COSTA, O. A.; ROÇA, R. O.; SILVEIRA, E. T. F.; ATHAYDE, N. B.; ARAÚJO, A. P.; MELLO JÚNIOR, A. |
Afiliação: |
OSMAR ANTONIO DALLA COSTA, CNPSA. |
Título: |
Bem-estar animal no transporte e a influência na qualidade da carne suína. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DE CARNES, 5., 2009, Garoa, SP. Anais... Garoa: ITAL/CTC, 2009. |
Idioma: |
Português |
Notas: |
Projeto/Plano de Ação: 02.06.10.100-03 |
Palavras-Chave: |
Carne suína. |
Thesagro: |
Qualidade; Transporte. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 00730naa a2200229 a 4500 001 1576132 005 2010-10-18 008 2009 bl --- 0-- u #d 100 1 $aLUDTKE, C. B. 245 $aBem-estar animal no transporte e a influência na qualidade da carne suína. 260 $c2009 500 $aProjeto/Plano de Ação: 02.06.10.100-03 650 $aQualidade 650 $aTransporte 653 $aCarne suína 700 1 $aDALLA COSTA, O. A. 700 1 $aROÇA, R. O. 700 1 $aSILVEIRA, E. T. F. 700 1 $aATHAYDE, N. B. 700 1 $aARAÚJO, A. P. 700 1 $aMELLO JÚNIOR, A. 773 $tIn: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DE CARNES, 5., 2009, Garoa, SP. Anais... Garoa: ITAL/CTC, 2009.
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