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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
13/05/2016 |
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Data da última atualização: |
11/10/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SCHWAB, S.; PESSOA, C. A.; BERGAMI, A. A. de L.; FIGUEIREDO, N. L. A.; TEIXEIRA, K. R. dos S.; BALDANI, J. I. |
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Afiliação: |
STEFAN SCHWAB, CNPAB; CRISTINA ALVES PESSOA, UFRJ; AMANDA APARECIDADE DE LIMA BERGAMI, UFRRJ; NATHÁLIA LIMA AZEVEDO FIGUEIREDO, UFRRJ; KATIA REGINA DOS SANTOS TEIXEIRA, CNPAB; JOSE IVO BALDANI, CNPAB. |
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Título: |
Isolation and characterization of active promoters from Gluconacetobacter diazotrophicus strain PAL5 using a promoter-trapping plasmid |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Archives of Microbiology, v. 198, n. 5, p. 445?458, 2016. |
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ISSN: |
1432-072X |
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DOI: |
10.1007/s00203-016-1203-y |
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Idioma: |
Inglês |
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Conteúdo: |
Gluconacetobacter diazotrophicus is a nitrogen-fixing, endophytic bacterium that has the potential to promote plant growth and increase yield. Genetically modified strains might get more benefits to host plants, including through expression of useful proteins, such as Cry toxins from B. thuringiensis, or enzymes involved in phytohormone production, proteins with antagonistic activity for phytopathogens, or that improve nutrient utilization by the plant. For that, expression systems for G. diazotrophicus are needed, which requires active promoters fused to foreign (or innate) genes. This article describes the construction of a G. diazotrophicus PAL5 promoter library using a promoter-less lacZ-bearing vector, and the identification of six active promoters through ?-galactosidase activity assays, sequencing and localization in the bacterial genome. The characterized promoters, which are located on distinct regions of the bacterial genome and encoding either sense or antisense transcripts, present variable expression strengths and might be used in the future for expressing useful proteins. |
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Palavras-Chave: |
Endophytic diazotroph; Plant growth promotion; Promoter trap; Protein expression system. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
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008 2016 bl uuuu u00u1 u #d
022 $a1432-072X
024 7 $a10.1007/s00203-016-1203-y$2DOI
100 1 $aSCHWAB, S.
245 $aIsolation and characterization of active promoters from Gluconacetobacter diazotrophicus strain PAL5 using a promoter-trapping plasmid
260 $c2016
520 $aGluconacetobacter diazotrophicus is a nitrogen-fixing, endophytic bacterium that has the potential to promote plant growth and increase yield. Genetically modified strains might get more benefits to host plants, including through expression of useful proteins, such as Cry toxins from B. thuringiensis, or enzymes involved in phytohormone production, proteins with antagonistic activity for phytopathogens, or that improve nutrient utilization by the plant. For that, expression systems for G. diazotrophicus are needed, which requires active promoters fused to foreign (or innate) genes. This article describes the construction of a G. diazotrophicus PAL5 promoter library using a promoter-less lacZ-bearing vector, and the identification of six active promoters through ?-galactosidase activity assays, sequencing and localization in the bacterial genome. The characterized promoters, which are located on distinct regions of the bacterial genome and encoding either sense or antisense transcripts, present variable expression strengths and might be used in the future for expressing useful proteins.
653 $aEndophytic diazotroph
653 $aPlant growth promotion
653 $aPromoter trap
653 $aProtein expression system
700 1 $aPESSOA, C. A.
700 1 $aBERGAMI, A. A. de L.
700 1 $aFIGUEIREDO, N. L. A.
700 1 $aTEIXEIRA, K. R. dos S.
700 1 $aBALDANI, J. I.
773 $tArchives of Microbiology$gv. 198, n. 5, p. 445?458, 2016.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
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Data corrente: |
13/12/2018 |
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Data da última atualização: |
06/06/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ANACLETO, P.; BARBOSA, V.; ALVES, R.; MAULVAULT, A. L.; FOGACA, F. H. dos S.; LANGERHOLC, T.; MARQUES, A. |
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Afiliação: |
Patrícia Anacleto; Vera Barbosa, Portuguese Institute for the Sea and Atmosphere (IPMA, I.P.); Ricardo Alves, Portuguese Institute for the Sea and Atmosphere (IPMA, I.P); Ana Luísa Maulvault, Portuguese Institute for the Sea and Atmosphere (IPMA, I.P.); FABIOLA HELENA DOS SANTOS FOGACA, CTAA; Tomaz Langerholc, Faculty of Agriculture and Life Sciences, University of Maribor; António Marques, Portuguese Institute for the Sea and Atmosphere (IPMA, I.P.). |
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Título: |
Use of green tea to reduce methylmercury bioaccessibility in raw and cooked fish. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
In: CONFERENCE OF THE WEST EUROPEAN FISH TECHNOLOGISTS'ASSOCIATION, 48., 2018, Lisbon. Agronegócio do pescado: desafios e oportunidades.Anais... São Paulo: Instituto da Pesca, 2018. |
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Idioma: |
Inglês |
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Notas: |
WEFTA. 15 a 18 out. 2018. |
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Thesagro: |
Tecnologia de Alimento. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/188345/1/Use-of-green-tea-to-reduce-methylmercury-bioaccessibility-Abstract-1.pdf
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Marc: |
LEADER 00735nam a2200193 a 4500
001 2101432
005 2023-06-06
008 2018 bl uuuu u00u1 u #d
100 1 $aANACLETO, P.
245 $aUse of green tea to reduce methylmercury bioaccessibility in raw and cooked fish.$h[electronic resource]
260 $aIn: CONFERENCE OF THE WEST EUROPEAN FISH TECHNOLOGISTS'ASSOCIATION, 48., 2018, Lisbon. Agronegócio do pescado: desafios e oportunidades.Anais... São Paulo: Instituto da Pesca$c2018
500 $aWEFTA. 15 a 18 out. 2018.
650 $aTecnologia de Alimento
700 1 $aBARBOSA, V.
700 1 $aALVES, R.
700 1 $aMAULVAULT, A. L.
700 1 $aFOGACA, F. H. dos S.
700 1 $aLANGERHOLC, T.
700 1 $aMARQUES, A.
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Embrapa Agroindústria de Alimentos (CTAA) |
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