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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
08/03/2016 |
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Data da última atualização: |
10/03/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
IVAMOTO, S. T.; DOMINGUES, D. S.; VIEIRA, L. G. E.; PEREIRA, L. F. P. |
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Afiliação: |
IAPAR; IAPAR; Universidade do Oeste Paulista; LUIZ FILIPE PROTASIO PEREIRA, SAPC. |
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Título: |
Identification of the transcriptionally active cytochrome P450 repertoire in Coffea arabica. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 14, n. 1, p. 2399-2412, 2015 |
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Idioma: |
Inglês |
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Conteúdo: |
Cytochrome P450s (P450s) comprise a gene superfamily encoding enzymes that are involved in diverse plant metabolic pathways that produce primary and secondary metabolites such as phenylpropanoids, terpenoids, nitrogen-containing compounds, and plant hormones. They comprise one of the most diverse gene families in plant evolution. Although there are many studies that aim to characterize P450s in plants, there is no report on the characterization of this superfamily in Coffea arabica, where they might be related to plant tolerance to biotic and abiotic stresses, as well as aroma-related compounds. In this study, we report the characterization and annotation of 87 putative P450s from C. arabica obtained from the Brazilian Coffee Genome Project and describe their transcriptional pattern in different tissues and coffee organs. To validate our approach, we measured the transcriptional profile of the CaCYP81D8_1 geneby quantitative polymerase chain reaction in leaves, flowers, and fruits.This study is the first effort to present and analyze the P450 superfamily in C. arabica, which may assist in understanding the chemical diversity of coffee secondary metabolites. |
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Palavras-Chave: |
Candidate genes; Expressed sequence tags (ESTs). |
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Thesagro: |
Coffea arábica. |
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Thesaurus Nal: |
Cytochrome P-450; Transcriptome. |
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Categoria do assunto: |
-- |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/1039979/1/Identificationofthetranscriptionally.pdf
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Marc: |
LEADER 01853naa a2200217 a 4500
001 2039979
005 2016-03-10
008 2015 bl uuuu u00u1 u #d
100 1 $aIVAMOTO, S. T.
245 $aIdentification of the transcriptionally active cytochrome P450 repertoire in Coffea arabica.$h[electronic resource]
260 $c2015
520 $aCytochrome P450s (P450s) comprise a gene superfamily encoding enzymes that are involved in diverse plant metabolic pathways that produce primary and secondary metabolites such as phenylpropanoids, terpenoids, nitrogen-containing compounds, and plant hormones. They comprise one of the most diverse gene families in plant evolution. Although there are many studies that aim to characterize P450s in plants, there is no report on the characterization of this superfamily in Coffea arabica, where they might be related to plant tolerance to biotic and abiotic stresses, as well as aroma-related compounds. In this study, we report the characterization and annotation of 87 putative P450s from C. arabica obtained from the Brazilian Coffee Genome Project and describe their transcriptional pattern in different tissues and coffee organs. To validate our approach, we measured the transcriptional profile of the CaCYP81D8_1 geneby quantitative polymerase chain reaction in leaves, flowers, and fruits.This study is the first effort to present and analyze the P450 superfamily in C. arabica, which may assist in understanding the chemical diversity of coffee secondary metabolites.
650 $aCytochrome P-450
650 $aTranscriptome
650 $aCoffea arábica
653 $aCandidate genes
653 $aExpressed sequence tags (ESTs)
700 1 $aDOMINGUES, D. S.
700 1 $aVIEIRA, L. G. E.
700 1 $aPEREIRA, L. F. P.
773 $tGenetics and Molecular Research$gv. 14, n. 1, p. 2399-2412, 2015
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Embrapa Café (CNPCa) |
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| 1. |  | SILVEIRA, S. V. da; BERLATTO, J. T.; PEREZ, E. A. P.; GUERRA, C. C.; OLIVEIRA, C. G. R. de; BEN, R. L.; PRETO, G. A. S. (ed.). Manual de boas práticas de fabricação/elaboração em vinícola compatível com a produção integrada de uva para processamento. Bento Gonçalves, RS: Embrapa Uva e Vinho, 2017. 52 il. color. (Embrapa Uva e Vinho. Documentos, 105).| Biblioteca(s): Embrapa Uva e Vinho. |
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