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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
15/02/2016 |
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Data da última atualização: |
26/09/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FANÉ, R.; TRAORÉ, D.; KASSOGUÉ, A.; SAMALÉ, F.; DICKO, A. H.; HAMADOUN, A.; VALICENTE, F. H.; BABANA, A. H. |
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Afiliação: |
FERNANDO HERCOS VALICENTE, CNPMS. |
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Título: |
Genetic diversity of native Bacillus thuringiensis strains isolated from soil of different localities in Mali and their cry gene profile. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
British Biotechnology Journal, v. 12, n. 1, p. 1-8, 2016. |
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DOI: |
10.9734/BBJ/2016/20432 |
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Idioma: |
Inglês |
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Conteúdo: |
Aims: To determine the genetic diversity of Bacillus thuringiensis strains isolated from soils of different locality in Mali, and select strains with cry1F, cry1B and cry1C genes to control caterpillars and strains with cry2 gene against African rice gall midge. Study Design: Strains of Bacillus thuringiensis (Bt) used in this study belong to collection of Laboratory of Research in Microbiology and Microbial Biotechnology (Laborem-Biotech) and that isolated from different ecological environment of Mali. Bt strains used as positive controls were kindly provided by Dr. Fernando Hercos Valicente from Embrapa Milho e Sorgo (Brazil). Methodology: The total DNA of the native B. thuringiensis and reference strains were extracted from overnight grown culture. Gene identification was performed by amplification (PCR) of DNA of Bt strains using specific primers. The gel revelation was performed using ethidium bromide and the gel photography was performed using an E-Box VX2 system, Version 15.06. Results: Native B. thuringiensis strains studied, showed high genetic diversity. 48,3% of the studied B. thuringiensis strains of the collection carry cry1 gene, 49,06% of them harbor both cry1 and cry2 gene, 5.7% of the native Bt strains didn?t react with any cry1 and cry 2 specific primers and 94.3% of the strains produce different PCR products. The analysis of cry1 positive B. thuringiensis showed sub-group frequencies of 7.6% for cry1F and 3.8% for cry1B and cry1C. In native Bt strains of our collection, the cry2 gene was always present with one or two cry1 sub-group(s). Conclusion: In this study, high genetic diversity in the native Bt strains from the bacterial collection in Mali was observed. Most of the native Bt strains studied harbor the cry1 gene only. In the cry1 gene profile, the cry1F sub-group was found to be the most frequently detected. None of studied Bt strains harbored the cry2 gene only. MenosAims: To determine the genetic diversity of Bacillus thuringiensis strains isolated from soils of different locality in Mali, and select strains with cry1F, cry1B and cry1C genes to control caterpillars and strains with cry2 gene against African rice gall midge. Study Design: Strains of Bacillus thuringiensis (Bt) used in this study belong to collection of Laboratory of Research in Microbiology and Microbial Biotechnology (Laborem-Biotech) and that isolated from different ecological environment of Mali. Bt strains used as positive controls were kindly provided by Dr. Fernando Hercos Valicente from Embrapa Milho e Sorgo (Brazil). Methodology: The total DNA of the native B. thuringiensis and reference strains were extracted from overnight grown culture. Gene identification was performed by amplification (PCR) of DNA of Bt strains using specific primers. The gel revelation was performed using ethidium bromide and the gel photography was performed using an E-Box VX2 system, Version 15.06. Results: Native B. thuringiensis strains studied, showed high genetic diversity. 48,3% of the studied B. thuringiensis strains of the collection carry cry1 gene, 49,06% of them harbor both cry1 and cry2 gene, 5.7% of the native Bt strains didn?t react with any cry1 and cry 2 specific primers and 94.3% of the strains produce different PCR products. The analysis of cry1 positive B. thuringiensis showed sub-group frequencies of 7.6% for cry1F and 3.8% for cry1B and cry1C. In native Bt strains of o... Mostrar Tudo |
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Palavras-Chave: |
Helicoverpa armegira. |
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Thesagro: |
Bacillus thuringiensis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02685naa a2200241 a 4500
001 2036996
005 2017-09-26
008 2016 bl uuuu u00u1 u #d
024 7 $a10.9734/BBJ/2016/20432$2DOI
100 1 $aFANÉ, R.
245 $aGenetic diversity of native Bacillus thuringiensis strains isolated from soil of different localities in Mali and their cry gene profile.$h[electronic resource]
260 $c2016
520 $aAims: To determine the genetic diversity of Bacillus thuringiensis strains isolated from soils of different locality in Mali, and select strains with cry1F, cry1B and cry1C genes to control caterpillars and strains with cry2 gene against African rice gall midge. Study Design: Strains of Bacillus thuringiensis (Bt) used in this study belong to collection of Laboratory of Research in Microbiology and Microbial Biotechnology (Laborem-Biotech) and that isolated from different ecological environment of Mali. Bt strains used as positive controls were kindly provided by Dr. Fernando Hercos Valicente from Embrapa Milho e Sorgo (Brazil). Methodology: The total DNA of the native B. thuringiensis and reference strains were extracted from overnight grown culture. Gene identification was performed by amplification (PCR) of DNA of Bt strains using specific primers. The gel revelation was performed using ethidium bromide and the gel photography was performed using an E-Box VX2 system, Version 15.06. Results: Native B. thuringiensis strains studied, showed high genetic diversity. 48,3% of the studied B. thuringiensis strains of the collection carry cry1 gene, 49,06% of them harbor both cry1 and cry2 gene, 5.7% of the native Bt strains didn?t react with any cry1 and cry 2 specific primers and 94.3% of the strains produce different PCR products. The analysis of cry1 positive B. thuringiensis showed sub-group frequencies of 7.6% for cry1F and 3.8% for cry1B and cry1C. In native Bt strains of our collection, the cry2 gene was always present with one or two cry1 sub-group(s). Conclusion: In this study, high genetic diversity in the native Bt strains from the bacterial collection in Mali was observed. Most of the native Bt strains studied harbor the cry1 gene only. In the cry1 gene profile, the cry1F sub-group was found to be the most frequently detected. None of studied Bt strains harbored the cry2 gene only.
650 $aBacillus thuringiensis
653 $aHelicoverpa armegira
700 1 $aTRAORÉ, D.
700 1 $aKASSOGUÉ, A.
700 1 $aSAMALÉ, F.
700 1 $aDICKO, A. H.
700 1 $aHAMADOUN, A.
700 1 $aVALICENTE, F. H.
700 1 $aBABANA, A. H.
773 $tBritish Biotechnology Journal$gv. 12, n. 1, p. 1-8, 2016.
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| 61. |  | FRANÇA, P. R. C. de; SILVA, O. R. R. F.; SOFIATTI, V.; ALVES, L. dos S. Desenvolvimento e avaliação de um equipamento para semeadura de SESAMUM INDICUM L. CONGRESSO BRASILEIRO DE MAMONA, 5.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 2.; FÓRUM CAPIXABA DE PINHÃO-MANSO, 1., 2012, Guarapari. Desafios e Oportunidades: anais. Campina Grande: Embrapa Algodão, 2012. p. 342| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Algodão. |
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| 62. | | MEDEIROS, J. da C.; SILVA, O. R. R. F. da; CARVALHO, O. S. Edafologia In: BELTRÃO, N. E. de M. O Agronegócio do algodão no Brasil. Campina Grande: Embrapa Algodão; Brasília: Embrapa Comunicação para Transferência de Tecnologia, 1999. v. 1 p. 119-171| Tipo: Capítulo em Livro Técnico-Científico | Circulação/Nível: -- - -- |
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| 64. | | CARTAXO, W. V.; SILVA, O. R. R. F.; SOFIATTI, V.; GONDIM, T. M. de S. Transferência de tecnologia e organização da produção. In: SANTOS, R. C. dos; FREIRE, R. M. M.; SUASSUNA, T. de M. F. (Ed.). Amendoim: o produtor pergunta, a Embrapa responde. Brasília, DF: Embrapa Informação Tecnológica: Campina Grande: Embrapa Algodão, 2009. p. 135-146| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Algodão. |
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| 70. | | FRANÇA. P. R. C. DE; SILVA, O. R. R. F.; ALMEIDA, F. DE A. C.; SOFIATTI, V.; ZONTA, J. H. Avaliação de uma câmara flambadora de algodão no deslintamento de sementes. In: CONGRESSO BRASILEIRO DE MAMONA, 6.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 3., 2014, Fortaleza. Energia e segurança alimentar na agricultura familiar: anais. Campina Grande, PB: Embrapa Algodão, 2014. p. 178| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Algodão. |
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| 75. | | JERÔNIMO, J. F.; FRANÇA, P. R. C. de; SILVA, O. R. R. F.; ALMEIDA, F. de A. C.; SOFIATTI, V. Comparação dos descaroçadores de 25 e 50 serras na qualidade tecnológica da fibra de algodão. CONGRESSO BRASILEIRO DE MAMONA, 5.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 2.; FÓRUM CAPIXABA DE PINHÃO-MANSO, 1., 2012, Guarapari. Desafios e Oportunidades: anais. Campina Grande: Embrapa Algodão, 2012. p. 338| Tipo: Resumo em Anais de Congresso |
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| 77. | | FRANÇA, P. R. C. DE; ALMEIDA, F. DE A. C.; SILVA, O. R. R. F.; FERREIRA, D. DA S. Germinação e vigor de sementes de gossypium hirsutum l. deslintadas por processo térmico. In: CONGRESSO BRASILEIRO DE MAMONA, 6.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 3., 2014, Fortaleza. Energia e segurança alimentar na agricultura familiar: anais. Campina Grande, PB: Embrapa Algodão, 2014. p. 236| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Algodão. |
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| 78. | | SILVA, O. R. R. F.; SOFIATTI, V.; SANTANA, J. C. F. de; WANDERLEY, M. J. R.; SANTOS, J. W. dos. Impacto do beneficiamento sobre o número de neps e quantidade de impurezas da fibra do algodão. Revista Brasileira de Engenharia Agrícola e Ambiental, v.14, n.1, p. 107-112, jan., 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
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| Registros recuperados : 224 | |
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