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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
29/01/2016 |
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Data da última atualização: |
01/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVARES, C. C. S.; FONSECA, J. F. da; CAMARGO, L. S. de A.; FABJAN, J. M. G. de S.; RODRIGUES, A. L. R.; BRANDÃO, F. Z. |
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Afiliação: |
Universidade Federal Fluminense; JEFERSON FERREIRA DA FONSECA, CNPC; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; Universidade Federal Fluminense; Universidade Federal Fluminense; Universidade Federal Fluminense. |
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Título: |
Comparison of different methods of goat sperm selection and capacitation for optimization of assisted reproductive technologies. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Small Ruminant Research v. 127, p. 44-49, June, 2015. |
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DOI: |
doi:10.1016/j.smallrumres.2015.04.009 |
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Idioma: |
Português |
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Conteúdo: |
This study evaluated different methods of frozen-thawed sperm selection and capacita-tion in goats for further use in reproductive biotechnologies. In Experiment 1, semen wasprocessed by the following techniques: mini-Percoll, swim-up, or washing by centrifuga-tion. In Experiment 2, mini-Percoll selected-sperm was subjected to capacitation inductionby incubation with: 50 ug/mL heparin, 10 uM, 50 uM, or 100 uM of sodium nitroprusside(SNP). Motility, vigor, acrosome, and plasma membrane (PM) integrity were evaluated afterthawing and after each treatment of sperm selection or capacitation. In Experiment 1, wash-ing by centrifugation presented greater (43%; P < 0.05) spermatozoa recovery rate than theother treatments. The swim-up technique showed the lowest (P < 0.05) progressive motil-ity (41%). Spermatozoa presenting both intact PM (P = 0.0002) and acrosome (P = 0.0004)showed an interaction effect between the buck and swim-up technique. In Experiment2, the addition of 100 M SNP resulted in greater (P < 0.05) motility and vigor (38%; 4.7),respectively, than did heparin (28%; 4.3). An enhancement (P < 0.05) in vigor was obtainedafter all treatments in comparison with the evaluation after thawing (3.3). In conclusion,mini-Percoll was better than swim-up for preparing frozen?thawed goat sperm, whereaswashing by centrifugation technique presented similar rates to mini-Percoll and could alsobe used. The use of 100 M SNP resulted in better motility and vigor than heparin treatment. |
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Palavras-Chave: |
Capacitation; Sodium nitroprusside; Sperm preparation; Sperm selection. |
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Thesagro: |
Caprino; Espermatozóide; Reprodução animal; Seleção; Sêmen. |
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Thesaurus Nal: |
goats; reproduction; spermatozoa. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
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100 1 $aOLIVARES, C. C. S.
245 $aComparison of different methods of goat sperm selection and capacitation for optimization of assisted reproductive technologies.$h[electronic resource]
260 $c2015
520 $aThis study evaluated different methods of frozen-thawed sperm selection and capacita-tion in goats for further use in reproductive biotechnologies. In Experiment 1, semen wasprocessed by the following techniques: mini-Percoll, swim-up, or washing by centrifuga-tion. In Experiment 2, mini-Percoll selected-sperm was subjected to capacitation inductionby incubation with: 50 ug/mL heparin, 10 uM, 50 uM, or 100 uM of sodium nitroprusside(SNP). Motility, vigor, acrosome, and plasma membrane (PM) integrity were evaluated afterthawing and after each treatment of sperm selection or capacitation. In Experiment 1, wash-ing by centrifugation presented greater (43%; P < 0.05) spermatozoa recovery rate than theother treatments. The swim-up technique showed the lowest (P < 0.05) progressive motil-ity (41%). Spermatozoa presenting both intact PM (P = 0.0002) and acrosome (P = 0.0004)showed an interaction effect between the buck and swim-up technique. In Experiment2, the addition of 100 M SNP resulted in greater (P < 0.05) motility and vigor (38%; 4.7),respectively, than did heparin (28%; 4.3). An enhancement (P < 0.05) in vigor was obtainedafter all treatments in comparison with the evaluation after thawing (3.3). In conclusion,mini-Percoll was better than swim-up for preparing frozen?thawed goat sperm, whereaswashing by centrifugation technique presented similar rates to mini-Percoll and could alsobe used. The use of 100 M SNP resulted in better motility and vigor than heparin treatment.
650 $agoats
650 $areproduction
650 $aspermatozoa
650 $aCaprino
650 $aEspermatozóide
650 $aReprodução animal
650 $aSeleção
650 $aSêmen
653 $aCapacitation
653 $aSodium nitroprusside
653 $aSperm preparation
653 $aSperm selection
700 1 $aFONSECA, J. F. da
700 1 $aCAMARGO, L. S. de A.
700 1 $aFABJAN, J. M. G. de S.
700 1 $aRODRIGUES, A. L. R.
700 1 $aBRANDÃO, F. Z.
773 $tSmall Ruminant Research$gv. 127, p. 44-49, June, 2015.
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