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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
18/09/2015 |
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Data da última atualização: |
24/04/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MARTINS, C. F.; FELICIANO SILVA, A. E. D.; DODE, M. A. N.; RUMPF, R.; CUMPA, H. C. B.; SILVA, C. G.; PIVATO, I. |
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Afiliação: |
CARLOS FREDERICO MARTINS, CPAC; MARGOT ALVES NUNES DODE, CENARGEN; RODOLFO RUMPF, CENARGEN. |
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Título: |
Morphological characterization and conservation of bovine spermatogenic cells by refrigeration at 4°C and freezing using different cryoprotective molecules. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Cryobiology, v. 71, p. 47-53, 2015. |
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ISSN: |
0011-2240 |
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DOI: |
https://doi.org/10.1016/j.cryobiol.2015.06.003 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The objectives of this study were study a practical method to characterize bovine spermatogenic cells and test the efficiency cells conservation by refrigeration at 4 °C and cryopreservation in different solutions using two cooling curves. Cellular identification was performing by analysis of shape, size and morphology, associated with nucleus positioning and nuclear-cytoplasm ratio (NCR). Cellular samples were kept at 4 °C for a period of 96 h in refrigeration solution and every 24 h plasma membrane and DNA integrity were evaluated. Cryopreservation of cells was carried out using solutions containing 10% Dimethyl sulfoxide, 5% Dimethylformamide, 7% Glycerol and 7% Ethylene glycol, using a controlled and non-controlled cooling curve. Results of cellular characterization demonstrated that spermatocytes II presented a cylindrical shape, NCR of 1:1.5 and diameter ranging from 14.5 to 17.5 μm. Round spermatids presented diameter ranging from 7.6 to 13.4 μm, acrosomal cap and NCR of 1:2. Elongation and elongated spermatids showed to marked divergence in shape. There was a daily significant loss of viability of cooled cells until third day of storage, however they presented 72.77 ± 5.16% viability after 4 days of storage at 4 °C. There was no difference among the cryoprotectant solutions and cooling curves. In conclusion we demonstrated that association of microscopes and staining was a practical method to identify bovine spermatogenic cells. Furthermore, refrigeration at 4 °C is an important strategy to preserve over 70% of viable cells after 4 days and cryopreservation, regardless of cryoprotectant solution or cooling curve used, can maintain over 50% of cells viable. MenosAbstract: The objectives of this study were study a practical method to characterize bovine spermatogenic cells and test the efficiency cells conservation by refrigeration at 4 °C and cryopreservation in different solutions using two cooling curves. Cellular identification was performing by analysis of shape, size and morphology, associated with nucleus positioning and nuclear-cytoplasm ratio (NCR). Cellular samples were kept at 4 °C for a period of 96 h in refrigeration solution and every 24 h plasma membrane and DNA integrity were evaluated. Cryopreservation of cells was carried out using solutions containing 10% Dimethyl sulfoxide, 5% Dimethylformamide, 7% Glycerol and 7% Ethylene glycol, using a controlled and non-controlled cooling curve. Results of cellular characterization demonstrated that spermatocytes II presented a cylindrical shape, NCR of 1:1.5 and diameter ranging from 14.5 to 17.5 μm. Round spermatids presented diameter ranging from 7.6 to 13.4 μm, acrosomal cap and NCR of 1:2. Elongation and elongated spermatids showed to marked divergence in shape. There was a daily significant loss of viability of cooled cells until third day of storage, however they presented 72.77 ± 5.16% viability after 4 days of storage at 4 °C. There was no difference among the cryoprotectant solutions and cooling curves. In conclusion we demonstrated that association of microscopes and staining was a practical method to identify bovine spermatogenic cells. Furthermore, refrigeration a... Mostrar Tudo |
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Thesagro: |
Congelamento; Criopreservação; Reprodução animal. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02529naa a2200253 a 4500
001 2024351
005 2025-04-24
008 2015 bl uuuu u00u1 u #d
022 $a0011-2240
024 7 $ahttps://doi.org/10.1016/j.cryobiol.2015.06.003$2DOI
100 1 $aMARTINS, C. F.
245 $aMorphological characterization and conservation of bovine spermatogenic cells by refrigeration at 4°C and freezing using different cryoprotective molecules.$h[electronic resource]
260 $c2015
520 $aAbstract: The objectives of this study were study a practical method to characterize bovine spermatogenic cells and test the efficiency cells conservation by refrigeration at 4 °C and cryopreservation in different solutions using two cooling curves. Cellular identification was performing by analysis of shape, size and morphology, associated with nucleus positioning and nuclear-cytoplasm ratio (NCR). Cellular samples were kept at 4 °C for a period of 96 h in refrigeration solution and every 24 h plasma membrane and DNA integrity were evaluated. Cryopreservation of cells was carried out using solutions containing 10% Dimethyl sulfoxide, 5% Dimethylformamide, 7% Glycerol and 7% Ethylene glycol, using a controlled and non-controlled cooling curve. Results of cellular characterization demonstrated that spermatocytes II presented a cylindrical shape, NCR of 1:1.5 and diameter ranging from 14.5 to 17.5 μm. Round spermatids presented diameter ranging from 7.6 to 13.4 μm, acrosomal cap and NCR of 1:2. Elongation and elongated spermatids showed to marked divergence in shape. There was a daily significant loss of viability of cooled cells until third day of storage, however they presented 72.77 ± 5.16% viability after 4 days of storage at 4 °C. There was no difference among the cryoprotectant solutions and cooling curves. In conclusion we demonstrated that association of microscopes and staining was a practical method to identify bovine spermatogenic cells. Furthermore, refrigeration at 4 °C is an important strategy to preserve over 70% of viable cells after 4 days and cryopreservation, regardless of cryoprotectant solution or cooling curve used, can maintain over 50% of cells viable.
650 $aCongelamento
650 $aCriopreservação
650 $aReprodução animal
700 1 $aFELICIANO SILVA, A. E. D.
700 1 $aDODE, M. A. N.
700 1 $aRUMPF, R.
700 1 $aCUMPA, H. C. B.
700 1 $aSILVA, C. G.
700 1 $aPIVATO, I.
773 $tCryobiology$gv. 71, p. 47-53, 2015.
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