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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
25/05/2015 |
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Data da última atualização: |
10/01/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVARES, C. C. S.; FONSECA, J. F. da; CAMARGO, L. S. de A.; SOUZA FABJAN, J. M. G. de; RODRIGUES, A. L. R.; BRANDÃO, F. Z. |
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Afiliação: |
CAROLINA CERQUEIRA SARMENTO OLIVARES, FLUMINENSE FEDERAL UNIVERSITY; JEFERSON FERREIRA DA FONSECA, CNPC; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; JOANNA MARIA GONÇALVES DE SOUZA-FABJAN, FLUMINENSE FEDERAL UNIVERSITY; ANDRÉ LUÍS RIOS RODRIGUES, FLUMINENSE FEDERAL UNIVERSITY; FELIPE ZANDONADI BRANDÃO, FLUMINENSE FEDERAL UNIVERSITY. |
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Título: |
Comparison of different methods of goat sperm selection and capacitation for optimization of assisted reproductive technologies. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Small Ruminant Research, v. 127, p. 44-49, June, 2015. |
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DOI: |
doi:10.1016/j.smallrumres.2015.04.009 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: tThis study evaluated different methods of frozen-thawed sperm selection and capacita-tion in goats for further use in reproductive biotechnologies. In Experiment 1, semen wasprocessed by the following techniques: mini-Percoll, swim-up, or washing by centrifuga-tion. In Experiment 2, mini-Percoll selected-sperm was subjected to capacitation inductionby incubation with: 50 ug/mL heparin, 10 uM, 50 uM, or 100 uM of sodium nitroprusside(SNP). Motility, vigor, acrosome, and plasma membrane (PM) integrity were evaluated afterthawing and after each treatment of sperm selection or capacitation. In Experiment 1, wash-ing by centrifugation presented greater (43%; P < 0.05) spermatozoa recovery rate than theother treatments. The swim-up technique showed the lowest (P < 0.05) progressive motil-ity (41%). Spermatozoa presenting both intact PM (P = 0.0002) and acrosome (P = 0.0004)showed an interaction effect between the buck and swim-up technique. In Experiment2, the addition of 100 M SNP resulted in greater (P < 0.05) motility and vigor (38%; 4.7),respectively, than did heparin (28%; 4.3). An enhancement (P < 0.05) in vigor was obtainedafter all treatments in comparison with the evaluation after thawing (3.3). In conclusion,mini-Percoll was better than swim-up for preparing frozen?thawed goat sperm, whereaswashing by centrifugation technique presented similar rates to mini-Percoll and could alsobe used. The use of 100 M SNP resulted in better motility and vigor than heparin treatment MenosAbstract: tThis study evaluated different methods of frozen-thawed sperm selection and capacita-tion in goats for further use in reproductive biotechnologies. In Experiment 1, semen wasprocessed by the following techniques: mini-Percoll, swim-up, or washing by centrifuga-tion. In Experiment 2, mini-Percoll selected-sperm was subjected to capacitation inductionby incubation with: 50 ug/mL heparin, 10 uM, 50 uM, or 100 uM of sodium nitroprusside(SNP). Motility, vigor, acrosome, and plasma membrane (PM) integrity were evaluated afterthawing and after each treatment of sperm selection or capacitation. In Experiment 1, wash-ing by centrifugation presented greater (43%; P < 0.05) spermatozoa recovery rate than theother treatments. The swim-up technique showed the lowest (P < 0.05) progressive motil-ity (41%). Spermatozoa presenting both intact PM (P = 0.0002) and acrosome (P = 0.0004)showed an interaction effect between the buck and swim-up technique. In Experiment2, the addition of 100 M SNP resulted in greater (P < 0.05) motility and vigor (38%; 4.7),respectively, than did heparin (28%; 4.3). An enhancement (P < 0.05) in vigor was obtainedafter all treatments in comparison with the evaluation after thawing (3.3). In conclusion,mini-Percoll was better than swim-up for preparing frozen?thawed goat sperm, whereaswashing by centrifugation technique presented similar rates to mini-Percoll and could alsobe used. The use of 100 M SNP resulted in better motility and vigor than heparin t... Mostrar Tudo |
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Palavras-Chave: |
Capacitation; Sodium nitroprusside; Sperm preparation; Sperm selection. |
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Thesagro: |
Caprino; Espermatozóide; Reprodução animal; Seleção; Sêmen. |
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Thesaurus Nal: |
Goats; Reproduction; Spermatozoa. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02541naa a2200337 a 4500
001 2016097
005 2025-01-10
008 2015 bl uuuu u00u1 u #d
024 7 $adoi:10.1016/j.smallrumres.2015.04.009$2DOI
100 1 $aOLIVARES, C. C. S.
245 $aComparison of different methods of goat sperm selection and capacitation for optimization of assisted reproductive technologies.$h[electronic resource]
260 $c2015
520 $aAbstract: tThis study evaluated different methods of frozen-thawed sperm selection and capacita-tion in goats for further use in reproductive biotechnologies. In Experiment 1, semen wasprocessed by the following techniques: mini-Percoll, swim-up, or washing by centrifuga-tion. In Experiment 2, mini-Percoll selected-sperm was subjected to capacitation inductionby incubation with: 50 ug/mL heparin, 10 uM, 50 uM, or 100 uM of sodium nitroprusside(SNP). Motility, vigor, acrosome, and plasma membrane (PM) integrity were evaluated afterthawing and after each treatment of sperm selection or capacitation. In Experiment 1, wash-ing by centrifugation presented greater (43%; P < 0.05) spermatozoa recovery rate than theother treatments. The swim-up technique showed the lowest (P < 0.05) progressive motil-ity (41%). Spermatozoa presenting both intact PM (P = 0.0002) and acrosome (P = 0.0004)showed an interaction effect between the buck and swim-up technique. In Experiment2, the addition of 100 M SNP resulted in greater (P < 0.05) motility and vigor (38%; 4.7),respectively, than did heparin (28%; 4.3). An enhancement (P < 0.05) in vigor was obtainedafter all treatments in comparison with the evaluation after thawing (3.3). In conclusion,mini-Percoll was better than swim-up for preparing frozen?thawed goat sperm, whereaswashing by centrifugation technique presented similar rates to mini-Percoll and could alsobe used. The use of 100 M SNP resulted in better motility and vigor than heparin treatment
650 $aGoats
650 $aReproduction
650 $aSpermatozoa
650 $aCaprino
650 $aEspermatozóide
650 $aReprodução animal
650 $aSeleção
650 $aSêmen
653 $aCapacitation
653 $aSodium nitroprusside
653 $aSperm preparation
653 $aSperm selection
700 1 $aFONSECA, J. F. da
700 1 $aCAMARGO, L. S. de A.
700 1 $aSOUZA FABJAN, J. M. G. de
700 1 $aRODRIGUES, A. L. R.
700 1 $aBRANDÃO, F. Z.
773 $tSmall Ruminant Research$gv. 127, p. 44-49, June, 2015.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| 5. |  | BALIGAR, V.; SICHER, R.; HE, Z.; FAGERIA, N.; SOUZA, J. O. de; AHNERT, D. Growth and micro nutrient nutrition of cacao influenced by source of iron. In: ASA-CSSA-SSSA INTERNATIONAL ANNUAL MEETINGS, 2011, San Antonio. Fundamental for life: soil, crop, and environmental sciences: abstracts. Madison: ASA: CSSA: SSSA, 2011.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 7. | | MACEDO, M. A.; MALIANIO, M. R.; ROJAS, M. R.; SOUZA, J. O.; INOUE-NAGATA, A. K.; GILBERTSON, R. L. Urther evidence of local evolution of weed-infecting begomoviruses in Brazil: molecular characterization and production of infectious clones of a new species infecting sida sp. from the state of Piauí. Virus Reviews and Research, Belo Horizonte, v. 20, p. 211-212, Oct. 2015. Supplement 1, ref. PIV 404. Edição dos resumos do XXVI Brazilian Congress of Virology, X Mercosur Meeting of Virology, 2015, Florianópolis.| Tipo: Resumo em Anais de Congresso |
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| 8. | | SILVA, B. S. da; CABREIRA, T. M.; De SOUZA, J. O.; MATIAS. N. R.; MACHADO, R. A. O.; JORGE, L. A. de C.; FERREIRA JR., P. R. Framework for Biological Control with Unmanned Aerial Vehicles. In: LATIN AMERICAN ROBOTIC SYMPOSIUM, 2022, BRAZILIAN SYMPOSIUM ON ROBOTICS (SBR), WORSHOP ON ROBOTICS IN EDUCATION (WRE), 2022, São Bernardo do Campo, Brazil. 25 - 30| Tipo: Artigo em Anais de Congresso |
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| 9. | | OLIVEIRA, V. R.; COSTA, N. D.; COSTA, C. A.; LEITE, D. L.; SANTOS, C. A. F.; THOMAZELLI, L. F.; VIDIGAL, S. M.; BREDA JUNIOR, J. M.; Z. FILHO, A.; LOPES, I. L.; SOUZA, J. O. Interação genótipo x ambiente na produção de cebola. Horticultura Brasileira, Brasília, DF, v. 26, n. 2, p. S5400-S5405, 2008. Suplemento. CD-ROM. Trabalho apresentado no 48. Congresso Brasileiro de OIericultura, Maringá, 2008.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Hortaliças. |
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| 10. | | OLIVEIRA, V. R.; COSTA, N. D.; COSTA, C. A. da; LEITE, D. L.; SANTOS, C. A. F.; THOMAZELLI, L. F.; VIDIGAL, S. M.; BREDA JÚNIOR, J. M.; Z. FILHO, A.; LOPES, I. L.; SOUZA, J. O. de. Interação genótipo x ambiente na produção de cebola. Horticultura Brasileira, Brasília, DF, v. 26, n. 2, p. S5400-5405, 2008. 1 CD-ROM. Suplemento. Edição dos Resumos do 48. Congresso Brasileiro de Olericultura, Maringá, jul. 2008.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Semiárido. |
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| 11. | | MAEDA, M. H. K.; KOYAMA, L. H. H.; CAMPOS, R. N. S.; KAUFFMANN, C. M.; SOUZA, J. O. de; GILBERTSON, R.; INOUE-NAGATA, A. K.; FREITAS, D. M. S.; NOGUEIRA, D. R. S.; MELO, F. L.; NAGATA, T. First report of watermelon crinkle leaf-associated virus 1 and 2 infecting watermelon (Citrullus lanatus) plants in Brazil. Plant Disease, v. 106, n. 2, p. 773, Feb. 2022.| Tipo: Nota Técnica/Nota Científica |
| Biblioteca(s): Embrapa Hortaliças; Embrapa Semiárido. |
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| 12. | | NAKASU, E. Y. T.; MELO, F. L.; NAGATA, T.; MICHEREFF FILHO, M.; SOUZA, J. O.; RIBEIRO, B. M.; RIBEIRO, S. G.; LACORTE, C.; PEREIRA, J. L.; NAGATA, A. K. I. Diversity of begomoviruses in the whitefly (Bemisia tabaci). Virus Reviews and Research, Belo Horizonte, v. 20, p. 199, Oct. 2015. Supplement 1, ref. PIV 247. Edição dos resumos do XXVI Brazilian Congress of Virology, X Mercosur Meeting of Virology, 2015, Florianópolis.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
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| 13. | | NAKASU, E. Y. T.; MELO, F. L.; NAGATA, T.; MICHEREFF FILHO, M.; SOUZA, J. O.; RIBEIRO, B. M.; RIBEIRO, S. G.; LACORTE, C.; PEREIRA, J. L.; INOUE-NAGATA, A. K. Discovery of potential entomopathogenic rna viruses in the whitefly (Bemisia Tabaci) using next generation sequencing. Virus Reviews and Research, Belo Horizonte, v. 20, p. 25-26, Oct. 2015. Supplement 1. Ref. PIV 246. Edição dos Resumos do XXVI Brazilian Congress of Virology, X Mercour Meeting of Virology, 2015, Florianópolis.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
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| 14. | | ROJAS, M. R.; MACEDO, M. A.; MALIANO, M. R.; SOTO-AGUILAR, M.; SOUZA, J. O.; BRIDDON, R. W.; KENYON, L.; BUSTAMANTE, R. F. R.; MURILO ZERBINI, F.; ADKINS, S.; LEGG, J. P.; KVARNHEDEN, A.; WINTERMANTEL, W. M.; SUDARSHANA, M. R.; PETERSCHMITT, M.; LAPIDOT, M.; MARTIN, D. P.; MORIONES, E.; INOUE-NAGATA, A. K.; GILBERTSON, R. L. World management of geminiviruses. Annual Review of Phytopathology, v. 56, p. 637-677, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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