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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
17/12/2014 |
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Data da última atualização: |
12/02/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BEZERRA, C. A.; MACEDO, L. L. P.; AMORIM, T. M. L.; SANTOS, V. O.; FRAGOSO, R. da R.; LUCENA, W. A.; MENEGUIM, A. M.; VALENCIA-JIMENEZ, A.; ENGLER, G.; SILVA, M. C. M. da; SA, M. F. G. de; FREIRE, E. V. S. A. |
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Afiliação: |
FURGS; FURGS; RODRIGO DA ROCHA FRAGOSO, CPAC; WAGNER ALEXANDRE LUCENA, CNPA; IAPAR; UNIVERSITY OF CALDAS, COLOMBIA; INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE, FRANCE; MARIA CRISTINA MATTAR DA SILVA, CENARGEN; MARIA FATIMA GROSSI DE SA, CENARGEN; ERIKA VALERIA SALIBA ALBUQUERQUE FR, CENARGEN. |
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Título: |
Molecular cloning and characterization of an a-amylase cDNA highly expressed in major feeding stages of the coffee berry borer, Hypothenemus hampei. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Gene, v. 553, n. 1, p. 7-16, Dec. 2014. |
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DOI: |
10.1016/j.gene.2014.09.050 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: a-Amylases are common enzymes responsible for hydrolyzing starch. Insect-pests, whose larvae develop in seeds, rely obligatorily on a-amylase activity to digest starch, as their major food source. Considering the relevance of insect a-amylases and the natural a-amylase inhibitors present in seeds to protect from insect damage, we report here the molecular cloning and nucleotide sequence of the full-length AmyHha cDNA of the coffee berry borer, Hypothenemus hampei, a major insect-pest of coffee crops. The AmyHha sequence has 1879 bp, containing a 1458 bp open reading frame, which encodes a predicted protein with 485 amino acid residues, with a predicted molecular mass of 51.2 kDa. The deduced protein showed 55?79% identity to other insect a-amylases, including Anthonomus grandis, Ips typographus and Sitophilus oryzae a-amylases. In depth analysis revealed that the highly conserved three amino acid residues (Asp184, Glu220, and Asp285), which compose the catalytic site are also presented in AmyHha amylase. The AmyHha gene seems to be a single copy in the haploid genome and AmyHha transcription levels were found higher in L2 larvae and adult insects, both corresponding to major feeding phases. Modeling of the AmyHha predicted protein uncovered striking structural similarities to the Tenebrio molitor a-amylase also displaying the same amino acid residues involved in enzyme catalysis (Asp184, Glu220 and Asp285). Since AmyHha gene was mostly transcribed in the intestinal tract of H. hampei larvae, the cognate a-amylase could be considered a high valuable target to coffee bean insect control by biotechnological strategies. MenosAbstract: a-Amylases are common enzymes responsible for hydrolyzing starch. Insect-pests, whose larvae develop in seeds, rely obligatorily on a-amylase activity to digest starch, as their major food source. Considering the relevance of insect a-amylases and the natural a-amylase inhibitors present in seeds to protect from insect damage, we report here the molecular cloning and nucleotide sequence of the full-length AmyHha cDNA of the coffee berry borer, Hypothenemus hampei, a major insect-pest of coffee crops. The AmyHha sequence has 1879 bp, containing a 1458 bp open reading frame, which encodes a predicted protein with 485 amino acid residues, with a predicted molecular mass of 51.2 kDa. The deduced protein showed 55?79% identity to other insect a-amylases, including Anthonomus grandis, Ips typographus and Sitophilus oryzae a-amylases. In depth analysis revealed that the highly conserved three amino acid residues (Asp184, Glu220, and Asp285), which compose the catalytic site are also presented in AmyHha amylase. The AmyHha gene seems to be a single copy in the haploid genome and AmyHha transcription levels were found higher in L2 larvae and adult insects, both corresponding to major feeding phases. Modeling of the AmyHha predicted protein uncovered striking structural similarities to the Tenebrio molitor a-amylase also displaying the same amino acid residues involved in enzyme catalysis (Asp184, Glu220 and Asp285). Since AmyHha gene was mostly transcribed in the intestinal... Mostrar Tudo |
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Palavras-Chave: |
Expressão gênica. |
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Thesagro: |
Amido; Curculionideo; Hidrolise; Praga de planta. |
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Thesaurus Nal: |
Curculionidae; Enzymatic hydrolysis; Gene expression; Insect pests; Starch. |
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Categoria do assunto: |
O Insetos e Entomologia |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/1003023/1/34279.pdf
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Marc: |
LEADER 02768naa a2200385 a 4500
001 2003023
005 2015-02-12
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1016/j.gene.2014.09.050$2DOI
100 1 $aBEZERRA, C. A.
245 $aMolecular cloning and characterization of an a-amylase cDNA highly expressed in major feeding stages of the coffee berry borer, Hypothenemus hampei.
260 $c2014
520 $aAbstract: a-Amylases are common enzymes responsible for hydrolyzing starch. Insect-pests, whose larvae develop in seeds, rely obligatorily on a-amylase activity to digest starch, as their major food source. Considering the relevance of insect a-amylases and the natural a-amylase inhibitors present in seeds to protect from insect damage, we report here the molecular cloning and nucleotide sequence of the full-length AmyHha cDNA of the coffee berry borer, Hypothenemus hampei, a major insect-pest of coffee crops. The AmyHha sequence has 1879 bp, containing a 1458 bp open reading frame, which encodes a predicted protein with 485 amino acid residues, with a predicted molecular mass of 51.2 kDa. The deduced protein showed 55?79% identity to other insect a-amylases, including Anthonomus grandis, Ips typographus and Sitophilus oryzae a-amylases. In depth analysis revealed that the highly conserved three amino acid residues (Asp184, Glu220, and Asp285), which compose the catalytic site are also presented in AmyHha amylase. The AmyHha gene seems to be a single copy in the haploid genome and AmyHha transcription levels were found higher in L2 larvae and adult insects, both corresponding to major feeding phases. Modeling of the AmyHha predicted protein uncovered striking structural similarities to the Tenebrio molitor a-amylase also displaying the same amino acid residues involved in enzyme catalysis (Asp184, Glu220 and Asp285). Since AmyHha gene was mostly transcribed in the intestinal tract of H. hampei larvae, the cognate a-amylase could be considered a high valuable target to coffee bean insect control by biotechnological strategies.
650 $aCurculionidae
650 $aEnzymatic hydrolysis
650 $aGene expression
650 $aInsect pests
650 $aStarch
650 $aAmido
650 $aCurculionideo
650 $aHidrolise
650 $aPraga de planta
653 $aExpressão gênica
700 1 $aMACEDO, L. L. P.
700 1 $aAMORIM, T. M. L.
700 1 $aSANTOS, V. O.
700 1 $aFRAGOSO, R. da R.
700 1 $aLUCENA, W. A.
700 1 $aMENEGUIM, A. M.
700 1 $aVALENCIA-JIMENEZ, A.
700 1 $aENGLER, G.
700 1 $aSILVA, M. C. M. da
700 1 $aSA, M. F. G. de
700 1 $aFREIRE, E. V. S. A.
773 $tGene$gv. 553, n. 1, p. 7-16, Dec. 2014.
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Registro original: |
Embrapa Cerrados (CPAC) |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
30/08/2018 |
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Data da última atualização: |
30/08/2018 |
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Autoria: |
SANTOS, A. C.; LEITE, F. P. L.; VIANNA, A. M.; WEEGE, G. B.; FINGER, I. S.; MULLER, V.; CURCIO, B. R.; NOGUEIRA, C. E. W. |
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Afiliação: |
Alice C. Santos, Faculdade de Veterinária/Universidade Federal de Pelotas - UFPel; Fábio P. L. Leite, Centro de Desenvolvimento Tecnológico - UFPel; Ana M. Vianna, Centro de Desenvolvimento Tecnológico - UFPel; Guilherme B. Weege, Laboratório de Parasitologia Molecular e Imunologia - UFPel; Ilusca S. Finger, Centro de Desenvolvimento Tecnológico - UFPel; Vitória Müller, Faculdade de Veterinária/Universidade Federal de Pelotas - UFPel; Bruna R. Curcio, Faculdade de Veterinária/Universidade Federal de Pelotas - UFPel; Carlos E. W. Nogueira, Faculdade de Veterinária/Universidade Federal de Pelotas - UFPel. |
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Título: |
Dynamics of humoral immune response in pregnant mares and foals vaccinated with Theileria equi recombinant EMA-2. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 6, p. 1105-1109, junho 2018 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Dinâmica da resposta imune humoral em éguas gestantes e potros vacinados com EMA-2 recombinante de Theileria equi. |
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Conteúdo: |
Theileria equi is an infectious hemoprotozoan agent of equine piroplasmosis, a disease that has severe economic and sanitary impact internationally. In addition to its common clinical features, piroplasmosis can cause gestational losses and neonatal damage, which makes neonates susceptible to this disease. The aim of this study was to evaluate the dynamics of humoral immune response to recombinant EMA-2 of T. equi in pregnant mares and foals, as well as the transfer of vaccine antibodies through the colostrum ingested by sucking foals. For vaccine production, the EMA-2 expression gene was cloned and expressed in the yeast species, Pichia pastoris. Thirty-six horses were used, of which 18 were pregnant mares and 18 were foals. The mares were divided into control and vaccinated groups, and the vaccinated group received three doses of rEMA-2 every 21 days starting at 300 days of gestation. Foals from vaccinated and control groups were evaluated until the sixth month of life. The production of antibodies by foals on the rEMA-2 vaccination schedule was also evaluated from the second month of life. Foals in the vaccinated group had received three doses of the vaccine every 21 days. The method used to evaluate serum and colostrum samples was indirect ELISA, and plates were sensitized with the rEMA-2 protein. At the end of the vaccination schedule, vaccinated mares showed a 2.3-fold increase in antibody levels when compared to baseline values. The colostrum of vaccinated mares presented antibody levels of 1.0432±0.33. Foals delivered by vaccinated mares presented levels of antibodies greater than those of foals delivered by control mares after their first time sucking (at about twelve hours after birth). Foals vaccinated in the second month of life showed an 8.3-fold increase in antibody levels when compared to baseline values. The vaccination schedule with rEMA-2 was able to stimulate humoral immunity in pregnant mares. Vaccine immunoglobins were concentrated in the colostrum of vaccinated mares and foals delivered by these mares showed an increase in serum levels of vaccine antibodies after the first-time sucking. MenosTheileria equi is an infectious hemoprotozoan agent of equine piroplasmosis, a disease that has severe economic and sanitary impact internationally. In addition to its common clinical features, piroplasmosis can cause gestational losses and neonatal damage, which makes neonates susceptible to this disease. The aim of this study was to evaluate the dynamics of humoral immune response to recombinant EMA-2 of T. equi in pregnant mares and foals, as well as the transfer of vaccine antibodies through the colostrum ingested by sucking foals. For vaccine production, the EMA-2 expression gene was cloned and expressed in the yeast species, Pichia pastoris. Thirty-six horses were used, of which 18 were pregnant mares and 18 were foals. The mares were divided into control and vaccinated groups, and the vaccinated group received three doses of rEMA-2 every 21 days starting at 300 days of gestation. Foals from vaccinated and control groups were evaluated until the sixth month of life. The production of antibodies by foals on the rEMA-2 vaccination schedule was also evaluated from the second month of life. Foals in the vaccinated group had received three doses of the vaccine every 21 days. The method used to evaluate serum and colostrum samples was indirect ELISA, and plates were sensitized with the rEMA-2 protein. At the end of the vaccination schedule, vaccinated mares showed a 2.3-fold increase in antibody levels when compared to baseline values. The colostrum of vaccinated mares prese... Mostrar Tudo |
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Palavras-Chave: |
Hemoprotozoan; Hemoprotozoário; Imunidade humoral; Piroplasmose equina; REMA-2. |
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Thesagro: |
Eqüino. |
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Thesaurus NAL: |
Equine piroplasmosis; Horses; Humoral immunity; Parasitoses; Theileria equi. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/182208/1/Dynamics-of-humoral-immune-response-in-pregnant-mares.pdf
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Marc: |
LEADER 03294naa a2200349 a 4500
001 2094866
005 2018-08-30
008 2018 bl uuuu u00u1 u #d
100 1 $aSANTOS, A. C.
245 $aDynamics of humoral immune response in pregnant mares and foals vaccinated with Theileria equi recombinant EMA-2.$h[electronic resource]
260 $c2018
500 $aTítulo em português: Dinâmica da resposta imune humoral em éguas gestantes e potros vacinados com EMA-2 recombinante de Theileria equi.
520 $aTheileria equi is an infectious hemoprotozoan agent of equine piroplasmosis, a disease that has severe economic and sanitary impact internationally. In addition to its common clinical features, piroplasmosis can cause gestational losses and neonatal damage, which makes neonates susceptible to this disease. The aim of this study was to evaluate the dynamics of humoral immune response to recombinant EMA-2 of T. equi in pregnant mares and foals, as well as the transfer of vaccine antibodies through the colostrum ingested by sucking foals. For vaccine production, the EMA-2 expression gene was cloned and expressed in the yeast species, Pichia pastoris. Thirty-six horses were used, of which 18 were pregnant mares and 18 were foals. The mares were divided into control and vaccinated groups, and the vaccinated group received three doses of rEMA-2 every 21 days starting at 300 days of gestation. Foals from vaccinated and control groups were evaluated until the sixth month of life. The production of antibodies by foals on the rEMA-2 vaccination schedule was also evaluated from the second month of life. Foals in the vaccinated group had received three doses of the vaccine every 21 days. The method used to evaluate serum and colostrum samples was indirect ELISA, and plates were sensitized with the rEMA-2 protein. At the end of the vaccination schedule, vaccinated mares showed a 2.3-fold increase in antibody levels when compared to baseline values. The colostrum of vaccinated mares presented antibody levels of 1.0432±0.33. Foals delivered by vaccinated mares presented levels of antibodies greater than those of foals delivered by control mares after their first time sucking (at about twelve hours after birth). Foals vaccinated in the second month of life showed an 8.3-fold increase in antibody levels when compared to baseline values. The vaccination schedule with rEMA-2 was able to stimulate humoral immunity in pregnant mares. Vaccine immunoglobins were concentrated in the colostrum of vaccinated mares and foals delivered by these mares showed an increase in serum levels of vaccine antibodies after the first-time sucking.
650 $aEquine piroplasmosis
650 $aHorses
650 $aHumoral immunity
650 $aParasitoses
650 $aTheileria equi
650 $aEqüino
653 $aHemoprotozoan
653 $aHemoprotozoário
653 $aImunidade humoral
653 $aPiroplasmose equina
653 $aREMA-2
700 1 $aLEITE, F. P. L.
700 1 $aVIANNA, A. M.
700 1 $aWEEGE, G. B.
700 1 $aFINGER, I. S.
700 1 $aMULLER, V.
700 1 $aCURCIO, B. R.
700 1 $aNOGUEIRA, C. E. W.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 38, n. 6, p. 1105-1109, junho 2018
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