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Biblioteca(s):  Embrapa Cerrados.
Data corrente:  17/12/2014
Data da última atualização:  12/02/2015
Tipo da produção científica:  Artigo em Periódico Indexado
Autoria:  BEZERRA, C. A.; MACEDO, L. L. P.; AMORIM, T. M. L.; SANTOS, V. O.; FRAGOSO, R. da R.; LUCENA, W. A.; MENEGUIM, A. M.; VALENCIA-JIMENEZ, A.; ENGLER, G.; SILVA, M. C. M. da; SA, M. F. G. de; FREIRE, E. V. S. A.
Afiliação:  FURGS; FURGS; RODRIGO DA ROCHA FRAGOSO, CPAC; WAGNER ALEXANDRE LUCENA, CNPA; IAPAR; UNIVERSITY OF CALDAS, COLOMBIA; INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE, FRANCE; MARIA CRISTINA MATTAR DA SILVA, CENARGEN; MARIA FATIMA GROSSI DE SA, CENARGEN; ERIKA VALERIA SALIBA ALBUQUERQUE FR, CENARGEN.
Título:  Molecular cloning and characterization of an a-amylase cDNA highly expressed in major feeding stages of the coffee berry borer, Hypothenemus hampei.
Ano de publicação:  2014
Fonte/Imprenta:  Gene, v. 553, n. 1, p. 7-16, Dec. 2014.
DOI:  10.1016/j.gene.2014.09.050
Idioma:  Inglês
Conteúdo:  Abstract: a-Amylases are common enzymes responsible for hydrolyzing starch. Insect-pests, whose larvae develop in seeds, rely obligatorily on a-amylase activity to digest starch, as their major food source. Considering the relevance of insect a-amylases and the natural a-amylase inhibitors present in seeds to protect from insect damage, we report here the molecular cloning and nucleotide sequence of the full-length AmyHha cDNA of the coffee berry borer, Hypothenemus hampei, a major insect-pest of coffee crops. The AmyHha sequence has 1879 bp, containing a 1458 bp open reading frame, which encodes a predicted protein with 485 amino acid residues, with a predicted molecular mass of 51.2 kDa. The deduced protein showed 55?79% identity to other insect a-amylases, including Anthonomus grandis, Ips typographus and Sitophilus oryzae a-amylases. In depth analysis revealed that the highly conserved three amino acid residues (Asp184, Glu220, and Asp285), which compose the catalytic site are also presented in AmyHha amylase. The AmyHha gene seems to be a single copy in the haploid genome and AmyHha transcription levels were found higher in L2 larvae and adult insects, both corresponding to major feeding phases. Modeling of the AmyHha predicted protein uncovered striking structural similarities to the Tenebrio molitor a-amylase also displaying the same amino acid residues involved in enzyme catalysis (Asp184, Glu220 and Asp285). Since AmyHha gene was mostly transcribed in the intestinal... Mostrar Tudo
Palavras-Chave:  Expressão gênica.
Thesagro:  Amido; Curculionideo; Hidrolise; Praga de planta.
Thesaurus Nal:  Curculionidae; Enzymatic hydrolysis; Gene expression; Insect pests; Starch.
Categoria do assunto:  O Insetos e Entomologia
URL:  https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/1003023/1/34279.pdf
Marc:  Mostrar Marc Completo
Registro original:  Embrapa Cerrados (CPAC)
Biblioteca ID Origem Tipo/Formato Classificação Cutter Registro Volume Status URL
CPAC34279 - 1UPCAP - PPS1773S1773
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Biblioteca(s):  Embrapa Unidades Centrais.
Data corrente:  30/08/2018
Data da última atualização:  30/08/2018
Autoria:  SANTOS, A. C.; LEITE, F. P. L.; VIANNA, A. M.; WEEGE, G. B.; FINGER, I. S.; MULLER, V.; CURCIO, B. R.; NOGUEIRA, C. E. W.
Afiliação:  Alice C. Santos, Faculdade de Veterinária/Universidade Federal de Pelotas - UFPel; Fábio P. L. Leite, Centro de Desenvolvimento Tecnológico - UFPel; Ana M. Vianna, Centro de Desenvolvimento Tecnológico - UFPel; Guilherme B. Weege, Laboratório de Parasitologia Molecular e Imunologia - UFPel; Ilusca S. Finger, Centro de Desenvolvimento Tecnológico - UFPel; Vitória Müller, Faculdade de Veterinária/Universidade Federal de Pelotas - UFPel; Bruna R. Curcio, Faculdade de Veterinária/Universidade Federal de Pelotas - UFPel; Carlos E. W. Nogueira, Faculdade de Veterinária/Universidade Federal de Pelotas - UFPel.
Título:  Dynamics of humoral immune response in pregnant mares and foals vaccinated with Theileria equi recombinant EMA-2.
Ano de publicação:  2018
Fonte/Imprenta:  Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 6, p. 1105-1109, junho 2018
Idioma:  Inglês
Notas:  Título em português: Dinâmica da resposta imune humoral em éguas gestantes e potros vacinados com EMA-2 recombinante de Theileria equi.
Conteúdo:  Theileria equi is an infectious hemoprotozoan agent of equine piroplasmosis, a disease that has severe economic and sanitary impact internationally. In addition to its common clinical features, piroplasmosis can cause gestational losses and neonatal damage, which makes neonates susceptible to this disease. The aim of this study was to evaluate the dynamics of humoral immune response to recombinant EMA-2 of T. equi in pregnant mares and foals, as well as the transfer of vaccine antibodies through the colostrum ingested by sucking foals. For vaccine production, the EMA-2 expression gene was cloned and expressed in the yeast species, Pichia pastoris. Thirty-six horses were used, of which 18 were pregnant mares and 18 were foals. The mares were divided into control and vaccinated groups, and the vaccinated group received three doses of rEMA-2 every 21 days starting at 300 days of gestation. Foals from vaccinated and control groups were evaluated until the sixth month of life. The production of antibodies by foals on the rEMA-2 vaccination schedule was also evaluated from the second month of life. Foals in the vaccinated group had received three doses of the vaccine every 21 days. The method used to evaluate serum and colostrum samples was indirect ELISA, and plates were sensitized with the rEMA-2 protein. At the end of the vaccination schedule, vaccinated mares showed a 2.3-fold increase in antibody levels when compared to baseline values. The colostrum of vaccinated mares prese... Mostrar Tudo
Palavras-Chave:  Hemoprotozoan; Hemoprotozoário; Imunidade humoral; Piroplasmose equina; REMA-2.
Thesagro:  Eqüino.
Thesaurus NAL:  Equine piroplasmosis; Horses; Humoral immunity; Parasitoses; Theileria equi.
Categoria do assunto:  --
URL:  https://ainfo.cnptia.embrapa.br/digital/bitstream/item/182208/1/Dynamics-of-humoral-immune-response-in-pregnant-mares.pdf
Marc:  Mostrar Marc Completo
Registro original:  Embrapa Unidades Centrais (AI-SEDE)
Biblioteca ID Origem Tipo/Formato Classificação Cutter Registro Volume Status
AI-SEDE62991 - 1UPEAP - DD
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