Data/hora: 25/04/2024 06:01:47

Biblioteca(s): 	Embrapa Semiárido.	
Data corrente: 	23/04/2003	
Data da última atualização: 	18/07/2019	
Autoria: 	HAWKER, J. S.	
Título: 	Insoluble invertase from grapes: an artifact of extraction.	
Ano de publicação: 	1969	
Fonte/Imprenta: 	Phytochemistry, v.8, p.337-344, 1969.	
Idioma: 	Inglês	
Conteúdo: 	Both soluble invertase (supematant from 10,000 g centrifugation) and insoluble invertase (precipitated with cell-wall fraction) were obtained from sultana grape berries homogenized under a variety of conditions. Treatment of the insoluble fraction with borate buffer, pH 8,5, polyethylene glycol (Carbowax4000) or non-ionic detergents solubilized most of the invertase. Homogenates of grapeberries prepared in the presence of Carbowax 4000, non-ionic detergents or protein (bovine serum albumin) contained up to 100 per cent of the total invertase in the soluble formo It is suggested that insoluble invertase from grapes is an artifact of extraction caused by the formation of a tannin-protein complex and/or a protein-tannin-cellwall complex. Invertase in grapes is probably located in the cytoplasm or vacuoles of cells. In contrast to grapes, insoluble invertase from aged aseptic carrot disks or from com coleoptiles could not be solubilized.	
Palavras-Chave: 	Artificial; Videira.	
Thesagro: 	Extrato; Uva.	
Categoria do assunto: 	F Plantas e Produtos de Origem Vegetal	
Registro original: 	Embrapa Semiárido (CPATSA)	
Exemplares: 	CPATSA - (26139-1) - ADD - SP - DD -  -  -  - DIS