Data/hora: 23/09/2024 11:32:37

Biblioteca(s): 	Embrapa Caprinos e Ovinos.	
Data corrente: 	13/04/2018	
Data da última atualização: 	29/01/2024	
Autoria: 	JAVIER MORENO, F.; RECIO, I.; OLANO, A.; LÓPEZ-FANDIÑO, R.	
Título: 	Chromatographic characterization of ovine kappa-casein macropeptide.	
Ano de publicação: 	2000	
Fonte/Imprenta: 	Journal of Dairy Research, v. 6, n. 3, p. 349-359, Aug. 2000.	
Idioma: 	Inglês	
Conteúdo: 	Abstract: Ovine casein macropeptide (CMP) was characterized by anion-exchange FPLC and reversed-phase (RP) HPLC. To study heterogeneity (the degree of glycosylation and phosphorylation), CMP was desialylated with neuraminidase and dephosphorylated with acid phosphatase. Following RP-HPLC, the main CMP components were identified using either on-line or off-line mass spectrometry. The most abundant ovine CMP component was a diphosphorylated carbohydrate-free form, followed by one or two monophosphorylated and a non-phosphorylated asialo-aglyco species. Aglyco non-phosphorylated, monophosphorylated and diphosphorylated forms were in the ratio 3[ratio ]20[ratio ]77. Only ? 30% of ovine CMP was glycosylated. Assuming that the monosaccharide fraction of ovine CMP is composed of N-acetylgalactosamine, galactose and N-glycolylneuraminic acid, molecular masses consistent with the presence of CMP containing tetra-, tri-, di- and monosaccharide were identified.	
Palavras-Chave: 	CMP; Milk protein; Neuraminidase; Ovine casein macropeptide.	
Thesaurus Nal: 	Acid phosphatase; Casein; Cattle; Glycosylation; Mass spectrometry; Peptides; Sheep.	
Categoria do assunto: 	L Ciência Animal e Produtos de Origem Animal	
Registro original: 	Embrapa Caprinos e Ovinos (CNPC)	
Exemplares: 	CNPC - (36907-1) - ADD - AP - DD -  -  -  - DIS