Data/hora: 05/05/2024 02:58:16

Biblioteca(s): | Embrapa Unidades Centrais.
Data corrente: | 18/06/2018
Data da última atualização: | 18/06/2018
Autoria: | ANDRADE, C. P.; BARBOSA NETO, J. D.; DRIEMEIER, D.
Afiliação: | Caroline P. Andrade; José D. Barbosa Neto; David Driemeier.
Título: | Identification of single nucleotide polymorphisms in the prion protein gene in Santa Ines and Dorset sheep.
Ano de publicação: | 2018
Fonte/Imprenta: | Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 4, p. 624-628, abril 2018
Idioma: | Inglês
Notas: | Título em português: Identificação de polimorfismos de nucleotídeos únicos em ovinos Santa Inês e Dorset através do gene da proteína priônica.
Conteúdo: | Scrapie is a transmissible spongiform encephalopathy (TSE) that affects sheep and goats and results from accumulation of the abnormal isoform of a prion protein in the central nervous system. Resistance or susceptibility to the disease is dependent on several factors, including the strain of infecting agent, the degree of exposure, and the presence of single nucleotide polymorphisms (SNPs) in the prion protein gene. The most important polymorphisms are present in codons 136, 154, and 171. SNPs have also been identified in other codons, such as 118, 127, 141, 142, and 143. The objective of this study was to investigate the genotypic profile of Santa Ines (n=94) and Dorset (n=69) sheep and identify polymorphisms in the prion protein gene using real-time PCR techniques and sequencing. We analyzed SNPs in 10 different codons (127, 136, 138, 140, 141, 142, 143, 154, 171, and 172) in Santa Ines sheep. Classification of the flock into risk groups associated with scrapie revealed that approximately 68% of the Santa Ines herd was considered at moderate risk (group 3), and the most frequent haplotype was ARQ/ARQ (47.8%). For Dorset sheep, 42% of the herd was considered at moderate risk (group 3), 40% at low risk (group 2), and 12% at very low risk (group 1). These findings improve our understanding of the genotype breed and further highlight the importance of genotyping and identification of polymorphisms in Brazilian herds to assess their effects on potential infections upon exposure to the sheep prion.
Palavras-Chave: | Genotipagem; Nulceotídeos únicos; Proteína priônica.
Thesagro: | Ovino; Polimorfismo.
Thesaurus Nal: | Genotyping; Sheep; Single nucleotide polymorphism.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/178696/1/Identification-of-single-nucleotide-polymorphisms.pdf
Registro original: | Embrapa Unidades Centrais (AI-SEDE)
Exemplares: | AI-SEDE - (62719-1) - UPE - AP - DD -  -  -  - DIS