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| 1. |  | SOUSA, W. J. B. de; ALMEIDA, K. V. de; RIBEIRO FILHO, N. M.; SILVA, F. L. H. da; FIRMINO, P. de T.; ROCHA, A. S. Produção de aguardente de laranja. In: CONGRESSO NORTE-NORDESTE DE QUÍMICA, 3.; ENCONTRO NORTE-NORDESTE DE ENSINO DE QUÍMICA, 1., 2009, São Luís.São Luís, 2009. 1 CD-ROM| Biblioteca(s): Embrapa Algodão. |
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| Registros recuperados : 1 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
22/05/2014 |
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Data da última atualização: |
02/04/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
NICKEL, O.; FAJARDO, T. V. M. |
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Afiliação: |
OSMAR NICKEL, CNPUV; THOR VINICIUS MARTINS FAJARDO, CNPUV. |
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Título: |
Detection of Viruses in Apples and Pears by Real Time RT-PCR Using 5'-Hydrolysis Probes |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Journal of Plant Pathology, v. 96, n. 1, p. 207-213, 2014. |
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Idioma: |
Inglês |
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Conteúdo: |
Apple stem pitting virus (ASPV), Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV) and Apple mosaic virus (ApMV) are common in apples and pears and the main targets of virus elimination from propagation material. The objective of this work was to design primers and probes for a real time RT-PCR protocol for detection of the four above viruses. FAM/TAMRA-labeled probes and primers were designed by searching for highly conserved nucleotide regions in the coat protein gene of the four viruses. Infection levels in analyzed apple samples were 92.6, 96.4, 100 and 88% for ASGV, ASPV, ACLSV and ApMV, respectively. In pears, all pre-existing ASPV infections were detected. Viral infections were confirmed in a selection of commercial cultivars of apples and pear scions, and quince rootstocks, demonstrating the sensitivity and reliability of the designed primers and probes. Real time RT-PCR using 5'-labeled probes is suitable for checking sanitary quality as a routine test in certification programs. |
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Palavras-Chave: |
RT-PCR. |
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Thesagro: |
DNA; Maça; Pera; Virus. |
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Thesaurus NAL: |
Apple chlorotic leaf spot virus; Apple mosaic virus; Apple stem grooving virus; Apple stem pitting virus; Fruit quality; Reverse transcriptase polymerase chain reaction. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/102459/1/JPP-2014-RT-PCR-Virus-macieira-Nickel-Thor.pdf
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Marc: |
LEADER 01817naa a2200265 a 4500
001 1986801
005 2019-04-02
008 2014 bl uuuu u00u1 u #d
100 1 $aNICKEL, O.
245 $aDetection of Viruses in Apples and Pears by Real Time RT-PCR Using 5'-Hydrolysis Probes$h[electronic resource]
260 $c2014
520 $aApple stem pitting virus (ASPV), Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV) and Apple mosaic virus (ApMV) are common in apples and pears and the main targets of virus elimination from propagation material. The objective of this work was to design primers and probes for a real time RT-PCR protocol for detection of the four above viruses. FAM/TAMRA-labeled probes and primers were designed by searching for highly conserved nucleotide regions in the coat protein gene of the four viruses. Infection levels in analyzed apple samples were 92.6, 96.4, 100 and 88% for ASGV, ASPV, ACLSV and ApMV, respectively. In pears, all pre-existing ASPV infections were detected. Viral infections were confirmed in a selection of commercial cultivars of apples and pear scions, and quince rootstocks, demonstrating the sensitivity and reliability of the designed primers and probes. Real time RT-PCR using 5'-labeled probes is suitable for checking sanitary quality as a routine test in certification programs.
650 $aApple chlorotic leaf spot virus
650 $aApple mosaic virus
650 $aApple stem grooving virus
650 $aApple stem pitting virus
650 $aFruit quality
650 $aReverse transcriptase polymerase chain reaction
650 $aDNA
650 $aMaça
650 $aPera
650 $aVirus
653 $aRT-PCR
700 1 $aFAJARDO, T. V. M.
773 $tJournal of Plant Pathology$gv. 96, n. 1, p. 207-213, 2014.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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