Data/hora: 05/07/2025 23:55:55

Biblioteca(s): 	Embrapa Caprinos e Ovinos.	
Data corrente: 	10/10/2002	
Data da última atualização: 	10/02/2017	
Autoria: 	SINGH, S. V.; GUPTA, V. K.; SINGH, N.	
Título: 	Comparative evaluation of a field-based dot-ELISA kit with three other serological tests for the detection of Brucella antibodies in goats.	
Ano de publicação: 	2000	
Fonte/Imprenta: 	Tropical Animal Health and Production, Edinburgh, v. 32, n. 3, p. 155-163, 2000.	
Idioma: 	Inglês	
Conteúdo: 	Abstract: A dot-ELISA (d-ELISA) test was evaluated and compared with the serum agg1utination test (SAT), micro-complement fixation test (CFT) and a plate-ELISA (p-ELISA) for field use in screening herds of goats against brucellosis. During the standardization of the dot-ELISA kit on 1732 caprine serum samples, 1571 samples out of 1666 were found to be negative in d-ELISA, SAT and micro-CFT, while 59 were positive in different combinations. Of a further 66 serum samples, 34 were negative and 31 were positive in different combinations in d-ELISA, SAT, micro-CFT and p-ELISA. A total of 1584 goats belonging to different herds were then screened for brucellosis. Of the 694 serum samples screened in the first batch using d-ELISA, a positive reaction was observed in 26 cases. Further screening of these cases revealed 13 and 21 goats as positive reactors in SAT and CFT, respectively. In a second batch of 890 goats there were 109 positive reactors in d-ELISA. Among these 109 goats, 34, 40 and 80 goats were positive reactors in SAT, CFT and p-ELISA, respectively. The results of d-ELISA correlated well with those of p-ELISA. Dot-ELISA was found to be a more suitable and rapid test for screening large numbers of goats in the field.	
Palavras-Chave: 	Dot-ELISA; Sorologia.	
Thesagro: 	Anticorpo; Brucelose; Caprino; Diagnostico; Doença Animal; Imunologia.	
Thesaurus Nal: 	Brucella melitensis.	
Categoria do assunto: 	H Saúde e Patologia	
Registro original: 	Embrapa Caprinos e Ovinos (CNPC)	
Exemplares: 	CNPC - (14959-1) - ADD - AP - PP -  -  -  - DIS