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| Registros recuperados : 15 | |
| 1. |  | TIEPO, A. N.; COUTINHO, I. D.; MACHADO, G. O.; CALZAVARA, A. K.; HERTEL, M. F.; PIMENTA, J. A.; OLIVEIRA, A. L. M. de; COLNAGO, L. A.; MERTZ-HENNING, L. M.; OLIVEIRA, H. C.; STOLF-MOREIRA, R. Infuence of plant growth‑promoting bacteria on leaf carbon and nitrogen metabolism of two drought‑stressed neotropical tree species: a metabolomic approach. Planta, v. 260, 31, 2024. 13 p.| Biblioteca(s): Embrapa Instrumentação; Embrapa Soja. |
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| 2. | | COUTINHO, I. D.; FACCHINATO, W. M.; MERTZ-HENNING, L. M.; VIANA, A. J. C.; MARIN, S. R. R.; SANTAGNELI, S. H.; NEPOMUCENO, A. L.; COLNAGO, L. A. NMR Fingerprinting of Conventional and Genetically Modified Soybean Plants with AtAREB1 Transcription Factors. ACS Omega, v. 9, 2024. 32651−32661| Biblioteca(s): Embrapa Instrumentação; Embrapa Soja. |
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| 3. | | CAMARGO, M. S.; COUTINHO, I. D.; LOURENÇO, S. A.; SOARES, M. K. M.; COLNAGO, L. A.; APPEZZATO-DA-GLÓRIA, B.; CAVALHEIRO, A. J.; AMORIM, L. Potential prophylactic role of silicon against brown rust (Puccinia melanocephala) in sugarcane. European Journal of Plant Pathology, v. 157, n. 1, 2020. 77 - 88| Biblioteca(s): Embrapa Instrumentação. |
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| 4. | | ANDREATA, E. C.; COUTINHO, I. D.; MARIN, S. R. R.; BELINATO, C. F.; MOLINARI, M. D. C.; BARBOSA, D. A.; NEPOMUCENO, A. L.; COLNAGO, L. A.; MERTZ-HENNING, L. M. ACETIC ACID APPLICATION TO ENHANCE DROUGHT TOLERANCE IN SOYBEAN In: Congresso Brasileiro de Genética, 65., 2019, Lindóia. [S. l.: s. n.], 2019. Ebook. p. 59.| Biblioteca(s): Embrapa Soja. |
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| 5. | | ANDREATA, E. C.; COUTINHO, I. D.; MARIN, S. R. R.; BELINATO, C. F.; MOLINARI, M. D. C.; BARBOSA, D. A.; NEPOMUCENO, A. L.; COLNAGO, L. A.; MERTZ-HENNING, L. M. Acetic acid application to enhance drought tolerance in soybean. In: CONGRESSO BRASILEIRO DE GENÉTICA, 65, Águas de Lindóia, SP, 2019. 59| Biblioteca(s): Embrapa Instrumentação. |
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| 6. | | MARINHO, J. P.; COUTINHO, I. D.; LAMEIRO, R. da F.; MARIN, S. R. R.; COLNAGO, L. A.; NAKASHIMA, K.; YAMAGUCHI-SHINOZAQUI, K.; NEPOMUCENO, A. L.; MERTZ-HENNING, L. M. Metabolic alterations in conventional and genetically modified soybean plants with GmDREB2A;2 FL and GmDREB2A;2 CA transcription factors during water deficit. Plant Physiology and Biochemistry, v. 140, p. 122-135, 2019.| Biblioteca(s): Embrapa Soja. |
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| 7. | | MARINHO, J. P.; COUTINHO, I. D.; LAMEIRO, R. F.; MARIN, S. R. R.; COLNAGO, L. A.; NAKASHIMA, K.; YAMAGUCH-SHINOZAKI, K.; NEPOMUCENO, A. L.; MERTZ-HENNING, L. M. Metabolic alterations in conventional and genetically modified soybean plants with GmDREB2A;2 FL and GmDREB2A;2 CA transcription factors during water deficit. Plant Physiology and Biochemistry v. 140, 2019. 122-135| Biblioteca(s): Embrapa Instrumentação; Embrapa Soja. |
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| 8. | | COUTINHO, I. D.; MERTZ-HENNING, L. M.; DOPP, S. A.; NEPOMUCENO, A. L.; MORAES, L. A. C.; MARCOLINO-GOMES, J.; RICHTER, C.; SCHWALBE, H.; COLNAGO, L. A. Identification of primary and secondary metabolites and transcriptome profile of soybean tissues during different stages of hypoxia. Data in Brief, v. 21, p. 1089-1100, 2018.| Biblioteca(s): Embrapa Instrumentação. |
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| 9. | | COUTINHO, I. D.; MERTZ-HENNING, L. M.; DÖPP, S. A.; NEPOMUCENO, A.; MORAES, L. A. C.; MARCOLINO-GOMES, J.; RICHTER, C.; SCHWALBE, H.; COLNAGO, L. A. Identification of primary and secondary metabolites and transcriptome profile of soybean tissues during different stages of hypoxia. Data in Brief, v. 21, p. 1089-100, 2018.| Biblioteca(s): Embrapa Soja. |
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| 11. | | COUTINHO, I. D.; MERTZ-HENNING, L. M.; DÖPP, S. A.; NEPOMUCENO, A.; MORAES, L. A. C.; MARCOLINO-GOMES, J.; RICHTER, C.; SCHWALBE, H.; COLNAGO, L. A. Flooded soybean metabolomic analysis reveals important primary and secondary metabolites involved in the hypoxia stress response and tolerance. Environmental and Experimental Botany, v. 153, p. 176-187, 2018.| Biblioteca(s): Embrapa Soja. |
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| 12. | | COUTINHO, I. D.; MERTZ-HENNING, L. M.; DOPP, S. A.; NEPOMUCENO, A.; MORAES, L. A. C.; MARCOLINO-GOMES, J.; RICHTER, C.; SCHWALBE, H.; COLNAGO, L. A. Flooded soybean metabolomic analysis reveals important primary and secondary metabolites involved involved in the hypoxia stress response and tolerance. Environmental and Experimental Botany, v. 153, p. 176-187, 2018.| Biblioteca(s): Embrapa Instrumentação. |
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| 13. | | COUTINHO, I. D.; HENNING, L. M. M.; NEPOMUCENO, A.; SANTAGNELID, S.; COLNAGO, L. A. High resolution magic angle spinning and solid-state NMR spectroscopy methods to explore the metabolome of soybean genotypes upon abiotic stress. In: II Latin-American Conference on Plant Phenotyping and Phenomics for Plant Breeding, 2, 2017, São Carlos, SP. Proceedings... São Carlos: Embrapa Instrumentação, p. 67, 2017.| Biblioteca(s): Embrapa Instrumentação. |
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| 14. | | CASTELLANE, T. C. L.; CAMPANHARO, J. C.; COLNAGO, L. A.; COUTINHO, I. D.; LOPES, E. M.; LEMOS, M. V. F.; LEMOS, E. G. M. Characterization of new exopolysaccharide production by Rhizobium tropici during growth on hidrocarbon substrate. In: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, n. 96, p. 361-369, 2017.| Biblioteca(s): Embrapa Instrumentação. |
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| 15. | | COUTINHO, I. D.; MORAES, T. B.; MERTZ-HENNING, L. M.; NEPOMUCENO, A. L.; GIORDANI, W.; MARCOLINO-GOMES, J.; SANTAGNELI, S.; COLNAGO, L. A. Integrating high-resolution and solid-state magic angle spinning NMR spectroscopy and a transcriptomic analysis of soybean tissues in response to water deficiency. In: Phytochemical Analysis, v. 28, p. 529-540, 2017.| Biblioteca(s): Embrapa Instrumentação; Embrapa Soja. |
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| Registros recuperados : 15 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
23/04/2003 |
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Data da última atualização: |
23/10/2019 |
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Autoria: |
ELOY, A. M. X. |
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Afiliação: |
ANGELA MARIA XAVIER ELOY, CNPC. |
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Título: |
Stress, opioid peptides and luteinizing hormone secretion in ewes. |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
1995. |
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Páginas: |
172 f. |
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Idioma: |
Inglês |
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Notas: |
Thesis (Doctor of Phylosophy) - University of Leeds, Leeds. |
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Conteúdo: |
The studies performed in this thesis aim to clarify the effects of physiologicaI and psychologicaI stresses on the plasma concentrations of cortisol and B-endorphin and the consequent effects on gonadotropin (LH) release in long-term ovariectomized ewes. AIso, a study was made of the. endogenous opioid involvement during application of these two kinds of stressors through the use of an opioid antagonist (naloxone). In this work not only was the peripheraI effect of naloxone evaluated but also its central action on the hypothalamo-pituitary adrenal axis activity and gonadotropin (LH) release. Plasma concentrations of B-endorphin and cortisol were elevated during acute (4 hours) insulin-induced hypoglycemia (2mU/kg/min) stress while plasma LH concentrations were inhibited. Infusion of the opioid antagonist naIoxone hydrochloride (5.5ug/kg/min) blocked the inhibitory effect of B-endorphin on LH release. It was concluded that endogenous opioids are involved in the control of LH release during hypoglycemia in ovariectomized ewes. In addition, this experiment detected no effect of acute hypoglycemia on episodic LH in ovariectomized animais. However, it was possible to observe a stimulatory effect of naIoxone on the LH pulse frequency in the first two hours after the beginning of the infusion, suggesting that this antagonist has short half life. During chronic (12 hours) insulin-induced hypoglycemia (2mU/kg/min), plasma ,B-endorphin levels retumed to basal levels while plasma cortisol concentrations showed a rise, suggesting incomplete feedback of corticosteroids on ACTH. LH levels remained low even during control treatment and naloxone (5.5Jlg/kg/min) infusion was not ~ble to stimulate the release of this hormone. In this case the long absence of ovarian steroids could be responsible for the lack of naloxone response and siso for the low levels of LH during saline infusion. The isolation stress that was used in this experiment allowed no visual contact but vocal communication was possible between the animaIs and lasted four hours. This caused a rise in the plasma {3-endorphin levels soon after the beginning of the stress procedure and a depression of the plasma LH leyels. Intravenous injection of naloxone (1 mg/kg) was capable of disinhibiting the LH response, suggesting the involvement of endogenous opioids in the control of LH during isolation stress. The isolation procedure did not significantly affect LH pulsatility. When the two stressors (hypoglycemia and isolation) were imposed together, plasma {3-endorphin concentrations Tose and plasma LH values were reduced. The LH pulse amplitude was affected suggesting a synergistic action of these two stressors on the GnRH pulse generator. When naIoxone (1 and 2 mg) was administered into the third ventricle to hypoglycemic animaIs made using insulin (1 mg/kg/i. v.) a blockade of the inhibitory effect of this stress on plasma LH concentrations was seen. However, naloxone had no effect on plasma LH when injected in animaIs under basal conditions. Plasma {3-endorphin and cortisol concentrations were depressed by i.c. v. injection of naloxone (2 mg) in hypoglycemic animais, but these hormones were not atfected in non-stressed animais. The intracerebroventricular injection of naloxone affected the LH pulse amplitude in hypoglycemic ewes. It is suggested that naloxone acts at the centraI nervous system, to regulate gonadotropin releasing hormone release in stressed animals. MenosThe studies performed in this thesis aim to clarify the effects of physiologicaI and psychologicaI stresses on the plasma concentrations of cortisol and B-endorphin and the consequent effects on gonadotropin (LH) release in long-term ovariectomized ewes. AIso, a study was made of the. endogenous opioid involvement during application of these two kinds of stressors through the use of an opioid antagonist (naloxone). In this work not only was the peripheraI effect of naloxone evaluated but also its central action on the hypothalamo-pituitary adrenal axis activity and gonadotropin (LH) release. Plasma concentrations of B-endorphin and cortisol were elevated during acute (4 hours) insulin-induced hypoglycemia (2mU/kg/min) stress while plasma LH concentrations were inhibited. Infusion of the opioid antagonist naIoxone hydrochloride (5.5ug/kg/min) blocked the inhibitory effect of B-endorphin on LH release. It was concluded that endogenous opioids are involved in the control of LH release during hypoglycemia in ovariectomized ewes. In addition, this experiment detected no effect of acute hypoglycemia on episodic LH in ovariectomized animais. However, it was possible to observe a stimulatory effect of naIoxone on the LH pulse frequency in the first two hours after the beginning of the infusion, suggesting that this antagonist has short half life. During chronic (12 hours) insulin-induced hypoglycemia (2mU/kg/min), plasma ,B-endorphin levels retumed to basal levels while plasma corti... Mostrar Tudo |
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Palavras-Chave: |
Hormônio luteinizante; LH; Secreção de hormônio. |
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Thesagro: |
Endocrinologia; Ovelha; Ovino; Reprodução Animal. |
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Thesaurus NAL: |
Animal nutrition; Animal physiology; Ewes; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 04171nam a2200265 a 4500
001 1529254
005 2019-10-23
008 1995 bl uuuu m 00u1 u #d
100 1 $aELOY, A. M. X.
245 $aStress, opioid peptides and luteinizing hormone secretion in ewes.
260 $a1995.$c1995
300 $a172 f.
500 $aThesis (Doctor of Phylosophy) - University of Leeds, Leeds.
520 $aThe studies performed in this thesis aim to clarify the effects of physiologicaI and psychologicaI stresses on the plasma concentrations of cortisol and B-endorphin and the consequent effects on gonadotropin (LH) release in long-term ovariectomized ewes. AIso, a study was made of the. endogenous opioid involvement during application of these two kinds of stressors through the use of an opioid antagonist (naloxone). In this work not only was the peripheraI effect of naloxone evaluated but also its central action on the hypothalamo-pituitary adrenal axis activity and gonadotropin (LH) release. Plasma concentrations of B-endorphin and cortisol were elevated during acute (4 hours) insulin-induced hypoglycemia (2mU/kg/min) stress while plasma LH concentrations were inhibited. Infusion of the opioid antagonist naIoxone hydrochloride (5.5ug/kg/min) blocked the inhibitory effect of B-endorphin on LH release. It was concluded that endogenous opioids are involved in the control of LH release during hypoglycemia in ovariectomized ewes. In addition, this experiment detected no effect of acute hypoglycemia on episodic LH in ovariectomized animais. However, it was possible to observe a stimulatory effect of naIoxone on the LH pulse frequency in the first two hours after the beginning of the infusion, suggesting that this antagonist has short half life. During chronic (12 hours) insulin-induced hypoglycemia (2mU/kg/min), plasma ,B-endorphin levels retumed to basal levels while plasma cortisol concentrations showed a rise, suggesting incomplete feedback of corticosteroids on ACTH. LH levels remained low even during control treatment and naloxone (5.5Jlg/kg/min) infusion was not ~ble to stimulate the release of this hormone. In this case the long absence of ovarian steroids could be responsible for the lack of naloxone response and siso for the low levels of LH during saline infusion. The isolation stress that was used in this experiment allowed no visual contact but vocal communication was possible between the animaIs and lasted four hours. This caused a rise in the plasma {3-endorphin levels soon after the beginning of the stress procedure and a depression of the plasma LH leyels. Intravenous injection of naloxone (1 mg/kg) was capable of disinhibiting the LH response, suggesting the involvement of endogenous opioids in the control of LH during isolation stress. The isolation procedure did not significantly affect LH pulsatility. When the two stressors (hypoglycemia and isolation) were imposed together, plasma {3-endorphin concentrations Tose and plasma LH values were reduced. The LH pulse amplitude was affected suggesting a synergistic action of these two stressors on the GnRH pulse generator. When naIoxone (1 and 2 mg) was administered into the third ventricle to hypoglycemic animaIs made using insulin (1 mg/kg/i. v.) a blockade of the inhibitory effect of this stress on plasma LH concentrations was seen. However, naloxone had no effect on plasma LH when injected in animaIs under basal conditions. Plasma {3-endorphin and cortisol concentrations were depressed by i.c. v. injection of naloxone (2 mg) in hypoglycemic animais, but these hormones were not atfected in non-stressed animais. The intracerebroventricular injection of naloxone affected the LH pulse amplitude in hypoglycemic ewes. It is suggested that naloxone acts at the centraI nervous system, to regulate gonadotropin releasing hormone release in stressed animals.
650 $aAnimal nutrition
650 $aAnimal physiology
650 $aEwes
650 $aSheep
650 $aEndocrinologia
650 $aOvelha
650 $aOvino
650 $aReprodução Animal
653 $aHormônio luteinizante
653 $aLH
653 $aSecreção de hormônio
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