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| Registros recuperados : 31 | |
| 5. |  | RODRIGUES, C. I.; COSTA, D. M. da; SANTOS, A. C. V.; BATATINHA, M. J. M.; SOUZA, F. V. D.; SOUZA, E. H. de; BOTURA, M. B.; ALVES, C. Q.; SOARES, T. L.; BRANDÃO, H. N. Assessment of in vitro anthelmintic activity and bio-guided chemical analysis of BRS Boyrá pineapple leaf extracts. Veterinary Parasitology, v.285, 2020.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 8. | | PEREIRA, A. da S.; COSTA, D. M. da; VENDRUSCOLO, J. L. da S.; DANIELS, J.; CHOER, E.; AUGUSTIN, E.; FORTES, G. R. de L.; BERTONCINI, O.; HIRANO, E.; GOMES, C. B. BRS Perola: cultivar de batata adaptada a sistema de producao de pequenos produtores, para processamento de batata palha. Agropecuaria Clima Temperado, Pelotas, v.3, n.2, p.287-290, dez. 2000.| Biblioteca(s): Embrapa Hortaliças. |
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| 10. | | TEODORO, A. V.; COSTA, D. M. da; BRITO, A. S.; SANTOS, S. S.; DOMPIERI, M. H. G.; SENA FILHO, J. G. de; BATISTA, M. C. Conservação da biodiversidade de abelhas e vespas solitárias em vegetação de restinga da Reserva Particular do Patrimônio Natural (RPPN) do Caju, Sergipe. Aracaju: Embrapa Tabuleiros Costeiros, 2019. Embrapa Tabuleiros Costeiros. Documentos, 222).| Biblioteca(s): Embrapa Tabuleiros Costeiros; Embrapa Territorial. |
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| 11. | | TEODORO, A. V.; BRITO, A. S. de; COSTA, D. M. da; DOMPIERI, M. H. G.; SENA FILHO, J. G. de; FARIAS, A. P.; JUMBO, L. O. V.; COELHO, C. R. Conservação da biodiversidade de besouros escarabeíneos (coleoptera: scarabaeidae) na Reserva Particular do Patrimônio Natural (RPPN) do Caju, Sergipe. Aracaju: Embrapa Tabuleiros Costeiros, 2019. (Embrapa Tabuleiros Costeiros. Documentos, 225).| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 13. | | SILVA, A. C. F. da; SOUZA, Z. da S.; MULLER, J. V.; VIZZOTTO, V. J.; REBELO, J. A.; ZANINI NETO, J. A.; COSTA, D. M. da; BERTONCINI, O. EPAGRI 361 - Catucha: nova cultivar de batata, especial para fritar. Horticultura Brasileira, Brasilia, v.14, n.1, p.61-62, maio 1996.| Biblioteca(s): Embrapa Hortaliças. |
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| 14. | | SILVA, A. C. F. da; MULLER, J. J. V.; VIZZOTTO, V. J.; REBELO, J. A.; SOUZA, Z. da S.; COSTA, D. M. da. EPAGRI 361-Catucha: nova cultivar de batata para Santa Catarina. Horticultura Brasileira, Brasilia, v.13, n.1, p.113, maio 1995. Resumo.| Biblioteca(s): Embrapa Hortaliças. |
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| 15. | | SILVA, A. C. F.; SOUZA, Z. da S.; MULLER, J. J. V.; VIZZOTO, V. J.; REBELO, J. A.; ZANINI NETO, J. A.; COSTA, D. M. da; BERTONCINI, O. EPAGRI 361-Catucha: nova cultivar de batata para Santa Catarina. Agropecuaria Catarinense, Florianopolis, v.8, n.3, p.22-25, set. 1995.| Biblioteca(s): Embrapa Hortaliças. |
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| 19. | | GALLI, J.; COSTA, D. M. da; LIBERAL, M. T.; LUZZARDI, G. C.; TYLER, K. B.; CASTRO, F. S. de. Experimentos de controle do Phytophthora infestans nas condições de Pelotas e São Lourenço do Sul, no Rio Grande do Sul. Pesquisa Agropecuária Brasileira, Rio de Janeiro, v. 5, p. 321-325, 1970. (Agronomia, 3). Título em inglês: Experiments in the control of Phytophthora infestans under the conditions of Pelotas and São Lourenço do Sul no Rio Grande do Sul, Brazil. A partir do v. 3(1968) é constituída de duas partes: Série Agronomia e Série...| Biblioteca(s): Embrapa Hortaliças; Embrapa Unidades Centrais. |
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| 20. | | GALLI, J.; COSTA, D. M. DA; LIBERAL, M. T.; LUZZARDI, G. C.; TYLER, K. B.; CASTRO, F. S. DE. Experimentos de controle do Phytophthora infestans nas condicoes de Pelotas e Sao Lourenco do Sul, no Rio Grande do Sul. Pesq. Agropec. Bras., v.5, p.321-325, 1970.| Biblioteca(s): Embrapa Agrobiologia. |
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| Registros recuperados : 31 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
05/02/2018 |
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Data da última atualização: |
06/12/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SILVA, A. S.; SEYFRIED, M.; CAMPESTRINI, L. H.; BAGGIO, S. F. Z.; MINHO, A. P.; MOLENTO, M. B.; MAURER, J. B. B. |
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Afiliação: |
Andressa Soldera Silva, UFPR; Melina Seyfried, UFPR; Luciano Henrique Campestrini, UFPR; Selma Faria Zawadzki Baggio, UFPR; ALESSANDRO PELEGRINE MINHO, CPPSE; Marcelo Beltrão Molento, UFPR; Juliana Bello Baron Maurer, UFPR. |
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Título: |
Assessment of anthelmintic activity and bio-guided chemical analysis of Persea americana seed extracts. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Veterinary Parasitology, v. 251, p. 34-43, feb. 2018. |
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DOI: |
10.1016/j.vetpar.2017.12.019 |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to characterize the extracts and fractions of Persea americana Mill. (Avocado) seeds and to determine the composition and the in vitro anthelmintic activity against third-stage larvae (L3) of Haemonchus contortus. The fresh (F) and dried (H) avocado seeds (PA) were subjected to extraction with hot water (W-PAF, W-PAH), ethanol (E-PAF, E-PAH) or methanol 70% (v/v), and partition with solvents of increasing polarity [n-hexane (H-PAF, H-PAH), chloroform (C-PAF, C-PAH), ethyl acetate (Ea-PAF, Ea-PAH), and n-butanol (B-PAF, B-PAH)], yielding a total of 14 extracts/fractions. After considering the yield, water solubility, and the preliminary results of the larval migration test (LMT), the E-PAF, E-PAH, H-PAF, and H-PAH were selected for further experiments. E-PAH presented an efficiency concentration of 50% (EC50) of 36 μg/mL on the LMT. E-PAH showed the greatest efficiency when its EC50 was compared to the other fractions (E-PAF = 147 μg/mL; H-PAF = 801 μg/mL; H-PAH = 77 μg/mL). After that, the E-PAH was chemically characterized, considering its quantitative polyphenolic and flavonoid contents by colorimetric and chromatographic techniques. E-PAH presented 50, 38, and 24 mg/g of dry matter of total phenol, condensed tannins (CT), and flavonoid contents, respectively. Using high performance liquid chromatography (HPLC) analysis, E-PAH had shown to have epicatechin (4.7 μg/mL), rutin (2.8 μg/mL), and chlorogenic acid (1.4 μg/mL) as its main constituents besides quercetin. These isolated compounds were evaluated using the LMT in order to relate the composition to the anthelmintic activity observed for E-PAH. Quercetin (EC50 = 7.8 μg/mL) and epicatechin (EC50 = 10 μg/mL) presented a higher efficiency than rutin (EC50 = 30 μg/mL). Chlorogenic acid was also tested with the LMT but did not present a significant efficiency. According to the results, the phenolic composition of E-PAH and the EC50 values obtained for the isolated phenols, it can be suggested that, besides the CT content, the presence of epicatechin and rutin contributed to the larvicidal activity of E-PAH. In conclusion, avocado seeds may be used as a source of polyphenols with promising anthelmintic applications. MenosThe aim of this study was to characterize the extracts and fractions of Persea americana Mill. (Avocado) seeds and to determine the composition and the in vitro anthelmintic activity against third-stage larvae (L3) of Haemonchus contortus. The fresh (F) and dried (H) avocado seeds (PA) were subjected to extraction with hot water (W-PAF, W-PAH), ethanol (E-PAF, E-PAH) or methanol 70% (v/v), and partition with solvents of increasing polarity [n-hexane (H-PAF, H-PAH), chloroform (C-PAF, C-PAH), ethyl acetate (Ea-PAF, Ea-PAH), and n-butanol (B-PAF, B-PAH)], yielding a total of 14 extracts/fractions. After considering the yield, water solubility, and the preliminary results of the larval migration test (LMT), the E-PAF, E-PAH, H-PAF, and H-PAH were selected for further experiments. E-PAH presented an efficiency concentration of 50% (EC50) of 36 μg/mL on the LMT. E-PAH showed the greatest efficiency when its EC50 was compared to the other fractions (E-PAF = 147 μg/mL; H-PAF = 801 μg/mL; H-PAH = 77 μg/mL). After that, the E-PAH was chemically characterized, considering its quantitative polyphenolic and flavonoid contents by colorimetric and chromatographic techniques. E-PAH presented 50, 38, and 24 mg/g of dry matter of total phenol, condensed tannins (CT), and flavonoid contents, respectively. Using high performance liquid chromatography (HPLC) analysis, E-PAH had shown to have epicatechin (4.7&#... Mostrar Tudo |
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Palavras-Chave: |
Avocado. |
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Thesagro: |
Haemonchus Contortus. |
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Thesaurus NAL: |
epicatechin. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03149naa a2200241 a 4500
001 2087019
005 2018-12-06
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1016/j.vetpar.2017.12.019$2DOI
100 1 $aSILVA, A. S.
245 $aAssessment of anthelmintic activity and bio-guided chemical analysis of Persea americana seed extracts.$h[electronic resource]
260 $c2018
520 $aThe aim of this study was to characterize the extracts and fractions of Persea americana Mill. (Avocado) seeds and to determine the composition and the in vitro anthelmintic activity against third-stage larvae (L3) of Haemonchus contortus. The fresh (F) and dried (H) avocado seeds (PA) were subjected to extraction with hot water (W-PAF, W-PAH), ethanol (E-PAF, E-PAH) or methanol 70% (v/v), and partition with solvents of increasing polarity [n-hexane (H-PAF, H-PAH), chloroform (C-PAF, C-PAH), ethyl acetate (Ea-PAF, Ea-PAH), and n-butanol (B-PAF, B-PAH)], yielding a total of 14 extracts/fractions. After considering the yield, water solubility, and the preliminary results of the larval migration test (LMT), the E-PAF, E-PAH, H-PAF, and H-PAH were selected for further experiments. E-PAH presented an efficiency concentration of 50% (EC50) of 36 μg/mL on the LMT. E-PAH showed the greatest efficiency when its EC50 was compared to the other fractions (E-PAF = 147 μg/mL; H-PAF = 801 μg/mL; H-PAH = 77 μg/mL). After that, the E-PAH was chemically characterized, considering its quantitative polyphenolic and flavonoid contents by colorimetric and chromatographic techniques. E-PAH presented 50, 38, and 24 mg/g of dry matter of total phenol, condensed tannins (CT), and flavonoid contents, respectively. Using high performance liquid chromatography (HPLC) analysis, E-PAH had shown to have epicatechin (4.7 μg/mL), rutin (2.8 μg/mL), and chlorogenic acid (1.4 μg/mL) as its main constituents besides quercetin. These isolated compounds were evaluated using the LMT in order to relate the composition to the anthelmintic activity observed for E-PAH. Quercetin (EC50 = 7.8 μg/mL) and epicatechin (EC50 = 10 μg/mL) presented a higher efficiency than rutin (EC50 = 30 μg/mL). Chlorogenic acid was also tested with the LMT but did not present a significant efficiency. According to the results, the phenolic composition of E-PAH and the EC50 values obtained for the isolated phenols, it can be suggested that, besides the CT content, the presence of epicatechin and rutin contributed to the larvicidal activity of E-PAH. In conclusion, avocado seeds may be used as a source of polyphenols with promising anthelmintic applications.
650 $aepicatechin
650 $aHaemonchus Contortus
653 $aAvocado
700 1 $aSEYFRIED, M.
700 1 $aCAMPESTRINI, L. H.
700 1 $aBAGGIO, S. F. Z.
700 1 $aMINHO, A. P.
700 1 $aMOLENTO, M. B.
700 1 $aMAURER, J. B. B.
773 $tVeterinary Parasitology$gv. 251, p. 34-43, feb. 2018.
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