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1. |  | ALENCAR, N. L. M.; ALVAREZ PIZARRO, J. C.; PRISCO, J. T.; BEZERRA, M. A.; GOMES FILHO, E. Alteração no potencial osmótico e nas concentrações de solutos orgânicos e inorgânicos em plântulas de cajueiro anão-precoce (anacardium occidentale L.) em resposta ao estresse salino. In: CONGRESSO BRASILEIRO DE FISIOLOGIA VEGETAL, 11, 2007, Gramado, RS. Resumos...Gramado: SBFV, 2007. Biblioteca(s): Embrapa Agroindústria Tropical. |
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2. |  | ALENCAR, N. L. M.; ALVAREZ PIZARRO, J. C.; PRISCO, J. T.; BEZERRA, M. A.; GOMES FILHO, E. Peroxidação de lipídios e atividade de enzimas antioxidativas em plântulas de cajueiro anão precoce (Anacardium occidentale L.) sob condições de estresse salino. In: CONGRESSO BRASILEIRO DE FISIOLOGIA VEGETAL, 11, 2007, Gramado, RS. Resumos...Gramado: SBFV, 2007. Biblioteca(s): Embrapa Agroindústria Tropical. |
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4. |  | ALVAREZ-PIZARRO, J. C.; GOMES FILHO, E.; PRISCO, J. T.; GROSSI DE SA, M. F.; OLIVEIRA NETO, O. B. de; FRAGOSO, R. da R. Plasma membrane H+-ATPase in sorghum roots as affected by potassium deficiency and nitrogen sources. Biologia Plantarum, v. 58, n. 3, p. 507-514, 2014. Biblioteca(s): Embrapa Cerrados. |
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5. |  | ALVAREZ-PIZARRO, J. C.; GOMES FILHO, E.; PRISCO, J. T.; GROSSI DE SA, M. F.; OLIVEIRA NETO, O. B. de; ROCHA FRAGOSO, R. da. Plasma membrane H+-ATPase in sorghum roots as affected by potassium deficiency and nitrogen sources. Biologia Plantarum, v. 58, n. 3, p. 507-514, 2014. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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6. |  | AMORIM, A. V.; GOMES FILHO, E.; PRISCO, J. T.; BEZERRA, M. A.; LACERDA, C. F. de; ALVAREZ PIZARRO, J. C.; NOGUEIRA, A. R. C. Produção e fisiologia de plantas de cajueiro anão precoce sob condições de sequeiro e irrigação. In: CONGRESSO BRASILEIRO DE FISIOLOGIA VEGETAL, 11, 2007, Gramado, RS. Resumos...Gramado: SBFV, 2007. Biblioteca(s): Embrapa Agroindústria Tropical. |
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Registros recuperados : 7 | |
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Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
15/08/2006 |
Data da última atualização: |
28/10/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
KASCHUK, G.; HUNGRIA, M.; ANDRADE, D. S.; CAMPO, R. J. |
Afiliação: |
MARIANGELA HUNGRIA DA CUNHA, CNPSO; RUBENS JOSE CAMPO, CNPSO. |
Título: |
Genetic diversity of rhizobia associated with common bean (Phaseolus vulgaris L.) grown under no-tillage and conventional systems in Southern Brazil. |
Ano de publicação: |
2006 |
Fonte/Imprenta: |
Applied Soil Ecology, v. 32, n. 2, p. 210-220, Jun. 2006. |
DOI: |
10.1016/j.apsoil.2005.06.008 |
Idioma: |
Inglês |
Conteúdo: |
Brazil is the largest producer and consumer of the common bean (Phaseolus vulgaris L.), but yields are often low and may be improved by a higher N supply through symbiosis with rhizobia. One main limitation to the N2-fixation process is the susceptibility of the symbiosis to environmental stresses frequent in the tropics, such as high soil temperatures and low soil moisture contents. Among other benefits, the no-tillage (NT) system reduces those stresses resulting in higher N2 fixation rates and yields; however, the effects of NT on rhizobial diversity are poorly understood. This study evaluated the diversity of rhizobia compatible with common bean in cropping areas under the NT or the conventional tillage (CT) systems in Ponta Grossa, State of Paraná, Southern Brazil. Genetic diversity was assessed by DNA analyses using the methodologies of BOX-PCR and RFLP-PCR of the 16S rDNA region. A high level of diversity was observed among the strains and the DNA profiles from the CT system were quite different from those from the NT system. Twenty-three RFLP-PCR profiles were obtained, indicating that many tropical rhizobial species remain to be described. Strain differentiation was achieved in the BOX-PCR analysis; diversity was slightly higher under the NT when compared with the CT system. Surprisingly, the rhizobial grouping based on cluster analysis of the RFLP-PCR of the 16S rDNA region indicated a higher diversity of species under the CT. It could be that the environmental stability offered by the NT system has led to a decrease in the number of species, with the predominance of the most successful ones, although genetic diversity within each species has increased. The results obtained in this study show that we still understand poorly the relation between microbial diversity and soil sustainability and that the complexity of the ecosystems require the evaluation of several parameters to define and monitor soil quality. MenosBrazil is the largest producer and consumer of the common bean (Phaseolus vulgaris L.), but yields are often low and may be improved by a higher N supply through symbiosis with rhizobia. One main limitation to the N2-fixation process is the susceptibility of the symbiosis to environmental stresses frequent in the tropics, such as high soil temperatures and low soil moisture contents. Among other benefits, the no-tillage (NT) system reduces those stresses resulting in higher N2 fixation rates and yields; however, the effects of NT on rhizobial diversity are poorly understood. This study evaluated the diversity of rhizobia compatible with common bean in cropping areas under the NT or the conventional tillage (CT) systems in Ponta Grossa, State of Paraná, Southern Brazil. Genetic diversity was assessed by DNA analyses using the methodologies of BOX-PCR and RFLP-PCR of the 16S rDNA region. A high level of diversity was observed among the strains and the DNA profiles from the CT system were quite different from those from the NT system. Twenty-three RFLP-PCR profiles were obtained, indicating that many tropical rhizobial species remain to be described. Strain differentiation was achieved in the BOX-PCR analysis; diversity was slightly higher under the NT when compared with the CT system. Surprisingly, the rhizobial grouping based on cluster analysis of the RFLP-PCR of the 16S rDNA region indicated a higher diversity of species under the CT. It could be that the environmental stab... Mostrar Tudo |
Palavras-Chave: |
Rizóbio. |
Thesagro: |
Feijão; Fixação de Nitrogênio. |
Thesaurus NAL: |
Beans; Nitrogen fixation. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 02686naa a2200229 a 4500 001 1469534 005 2024-10-28 008 2006 bl uuuu u00u1 u #d 024 7 $a10.1016/j.apsoil.2005.06.008$2DOI 100 1 $aKASCHUK, G. 245 $aGenetic diversity of rhizobia associated with common bean (Phaseolus vulgaris L.) grown under no-tillage and conventional systems in Southern Brazil.$h[electronic resource] 260 $c2006 520 $aBrazil is the largest producer and consumer of the common bean (Phaseolus vulgaris L.), but yields are often low and may be improved by a higher N supply through symbiosis with rhizobia. One main limitation to the N2-fixation process is the susceptibility of the symbiosis to environmental stresses frequent in the tropics, such as high soil temperatures and low soil moisture contents. Among other benefits, the no-tillage (NT) system reduces those stresses resulting in higher N2 fixation rates and yields; however, the effects of NT on rhizobial diversity are poorly understood. This study evaluated the diversity of rhizobia compatible with common bean in cropping areas under the NT or the conventional tillage (CT) systems in Ponta Grossa, State of Paraná, Southern Brazil. Genetic diversity was assessed by DNA analyses using the methodologies of BOX-PCR and RFLP-PCR of the 16S rDNA region. A high level of diversity was observed among the strains and the DNA profiles from the CT system were quite different from those from the NT system. Twenty-three RFLP-PCR profiles were obtained, indicating that many tropical rhizobial species remain to be described. Strain differentiation was achieved in the BOX-PCR analysis; diversity was slightly higher under the NT when compared with the CT system. Surprisingly, the rhizobial grouping based on cluster analysis of the RFLP-PCR of the 16S rDNA region indicated a higher diversity of species under the CT. It could be that the environmental stability offered by the NT system has led to a decrease in the number of species, with the predominance of the most successful ones, although genetic diversity within each species has increased. The results obtained in this study show that we still understand poorly the relation between microbial diversity and soil sustainability and that the complexity of the ecosystems require the evaluation of several parameters to define and monitor soil quality. 650 $aBeans 650 $aNitrogen fixation 650 $aFeijão 650 $aFixação de Nitrogênio 653 $aRizóbio 700 1 $aHUNGRIA, M. 700 1 $aANDRADE, D. S. 700 1 $aCAMPO, R. J. 773 $tApplied Soil Ecology$gv. 32, n. 2, p. 210-220, Jun. 2006.
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